Advanced Biomedical Research最新文献_第9页

Analysis of a four-component competing endogenous RNA network reveals potential biomarkers in gastric cancer: An integrated systems biology and experimental investigation 四组分竞争内源性RNA网络的分析揭示了胃癌的潜在生物标志物:综合系统生物学和实验研究

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_185_23

Parvaneh Nikpour, Sadra Salehi-Mazandarani

{"title":"Analysis of a four-component competing endogenous RNA network reveals potential biomarkers in gastric cancer: An integrated systems biology and experimental investigation","authors":"Parvaneh Nikpour, Sadra Salehi-Mazandarani","doi":"10.4103/abr.abr_185_23","DOIUrl":"https://doi.org/10.4103/abr.abr_185_23","url":null,"abstract":"Background: Gastric cancer (GC) is a common and deadly cancer worldwide. Molecular changes underlying the development of GC are not thoroughly understood. Therefore, we constructed and analyzed a novel four-component competing endogenous RNA (ceRNA) network to introduce plausible diagnostic and prognostic biomarkers in GC. Materials and Methods: Transcriptomics and circular RNA (circRNA) data were retrieved from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases, respectively. After batch effect correction, differential expression analysis, and interaction prediction, a ceRNA network including long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs) was established. Enrichment analyses were performed, and a protein–protein interaction (PPI) network was constructed. Furthermore, a subnetwork was extracted, and using the quantitative real-time polymerase chain reaction (qRT-PCR) method, the expression changes of two hub ceRNAs were examined. Finally, survival analysis was performed to identify potential prognostic RNAs. Results: A four-component ceRNA network containing 822 nodes and 1365 edges was constructed. Enrichment analyses unveiled important signaling pathways and gene ontologies such as neuroactive ligand–receptor interaction and axonogenesis. The PPI network showed the interactions among mRNAs of the ceRNA network. qRT-PCR indicated downregulation of EPHA5 and SNAP91 mRNAs in GC compared to control tissues. Survival analyses revealed eight mRNAs and one lncRNA as potential prognostic biomarkers in GC. Conclusion: The established four-component network of ceRNAs in GC reveals a comprehensive view of the molecular and cellular characteristics of GC progression, which can be considered as a basis to examine and validate potential diagnostic and prognostic biomarkers as well as therapeutic targets.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135317891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular and antigen detection, phylogenetics, and immunoinformatics study of the zoonotic coronavirus in Iranian diarrheic calves 伊朗腹泻小牛人畜共患冠状病毒的分子和抗原检测、系统发育和免疫信息学研究

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_422_22

MohammadM Ranjbar, Soodeh Enayati, Soodeh Hooshmandi, Shahrzad Ahangarzadeh, Shima Aboutalebian

{"title":"Molecular and antigen detection, phylogenetics, and immunoinformatics study of the zoonotic coronavirus in Iranian diarrheic calves","authors":"MohammadM Ranjbar, Soodeh Enayati, Soodeh Hooshmandi, Shahrzad Ahangarzadeh, Shima Aboutalebian","doi":"10.4103/abr.abr_422_22","DOIUrl":"https://doi.org/10.4103/abr.abr_422_22","url":null,"abstract":"Background: Bovine coronaviruses (BCoVs) are zoonotic diseases that result in substantial economic losses due to mortality, impaired growth, and increased medication expenses in large animals. These viruses pose a risk to children who live beside infected animal, as they can cause diarrhea. This study was dedicated to molecular and antigen detections and phylogenetic and immunoinformatics analysis of zoonotic coronavirus (CoV) in Iran.Materials and Methods: A total of 77 diarrheic samples were collected from Holstein dairy herds in selected provinces of Iran. Samples were tested by capture antibody enzyme-linked immunosorbent assay (ELISA) to detect CoV and reverse transcriptase–polymerase chain reaction (RT–PCR) for verification of detection and also genotyping of spike glycoprotein in CoV-positive samples. After statistical analysis, nucleotide sequence alignment, and nucleotide and protein phylogenetic tree construction, the centralized sequence for vaccine strains was obtained using computationally optimized broadly reactive antigen (COBRA)'s center-of-the-tree (COT) method.Results: Twenty-two (28.5%) and eight (10.3%) of 77 samples were positive according to RT–PCR and ELISA, respectively. (Basic Local Alignment Search Tool) BLAST and phylogenetic analysis revealed that most similar sequences to the Iranian CoV sequence were for European countries. Furthermore, there were strong correlations to other CoVs in humans and wild and domesticated animals. As CoV has variable COT, the most recent strains and COBRA vaccine strains were obtained.Conclusion: Based on the high prevalence of this viral disease in calves and its economic impact on the breeding industry, as well as the potential transmission to humans and correlation with World Health Organization (WHO) One Health approach guidelines, the study emphasizes the importance of implementing preventive strategies such as animal vaccination.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135799394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the effectiveness of conventional prostheses against dual-mobility prostheses in the treatment of femoral neck fractures in two separate groups of patients: A clinical trial study 评估两组患者股骨颈骨折常规假体与双活动假体的疗效:一项临床试验研究

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_108_23

Mehdi Motififard, Mohammad Parhamfar, Alireza Hosseini, Mostafa Khashei, Amirmohammad Taravati, Somayeh Shirazinejad, Ali Afsharirad, Amirhossein Sadeghian, AliSherafati Chaleshtori

{"title":"Evaluating the effectiveness of conventional prostheses against dual-mobility prostheses in the treatment of femoral neck fractures in two separate groups of patients: A clinical trial study","authors":"Mehdi Motififard, Mohammad Parhamfar, Alireza Hosseini, Mostafa Khashei, Amirmohammad Taravati, Somayeh Shirazinejad, Ali Afsharirad, Amirhossein Sadeghian, AliSherafati Chaleshtori","doi":"10.4103/abr.abr_108_23","DOIUrl":"https://doi.org/10.4103/abr.abr_108_23","url":null,"abstract":"Background: Femoral neck fracture is a common posttraumatic injury in the elderly. Currently, orthopedic surgeons conducting total hip arthroplasty (THA) for hip fractures have various prosthetic alternatives. Dual-mobility cup THA is recommended for hip fracture patients to reduce prosthesis dislocation. Therefore, the goal of this research is to evaluate the effectiveness of conventional prostheses against dual-mobility prostheses in treating femoral neck fractures in two groups of patients.Materials and Methods: The current clinical investigation involved patients with femoral neck fractures. Among approved 84 patients, 44 and 40 were undergoing conventional THA (group A) and dual-mobility THA (group B), respectively. Patients were observed regularly after surgeries, and the same measurements were performed. The data were assembled and entered into SPSS software (version 25, IBM Corporation, Armonk, NY, USA) and analyzed. In all analyses, a P value of less than 0.05 was considered significant.Results: Eighty-four cases with an average age of 63.97 years were evaluated. In postoperative follow-up, there were no statistically significant differences between the groups in terms of Harris hip score, SF-36, and infection (P > 0.05). However, patients who received conventional implants had more dislocation postoperatively (P = 0.045). Regarding the rehabilitation costs, there were no statistically significant differences between the groups, so the group using the conventional implants incurred a higher cost (P = 0.041).Conclusion: According to the results, both dual mobility and conventional implants had optimal functional outcomes in patients with femoral neck fractures.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135799838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Vitamin D Supplement on the Relapsing Incidence of Rhinosinusitis with Nasal Polyposis after Fuctional Endoscpoic Sinus Surgery. 补充维生素D对鼻窦炎合并鼻息肉术后复发率的影响。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_237_21

Hossein Ghazavi, Sayed Mostafa Hashemi, Samaneh Jafari

{"title":"The Effect of Vitamin D Supplement on the Relapsing Incidence of Rhinosinusitis with Nasal Polyposis after Fuctional Endoscpoic Sinus Surgery.","authors":"Hossein Ghazavi, Sayed Mostafa Hashemi, Samaneh Jafari","doi":"10.4103/abr.abr_237_21","DOIUrl":"https://doi.org/10.4103/abr.abr_237_21","url":null,"abstract":"Background: Nasal polyp (NP) is the most common benign tumor that can cause nasal obstruction and more annoying problems in patients. Recently, investigators have been focusing on complementary therapies alone or in conjunction with endoscopic nasal and sinus surgery. However, given the association of Vitamin D (VD) deficiency and chronic rhinosinusitis with nasal polyposis (CRSwNP) in previous studies, it may be possible to prevent the recurrence of NP and the development of rhinosinusitis by controlling serum levels of VD and maintaining it at a normal level. The current study aimed to investigate the efficacy of VD supplementation in preventing CRSwNP recurrence after endoscopic surgery.Materials and methods: This clinical trial composed of vitamin D deficient patients with CRSwNP who were candidates for endoscopic sinus surgery in two groups of cases and controls. After endoscopic sinus surgery for all patients, we administered VD supplementation (50,000 IU) once a week for 8 weeks for cases and no further intervention for controls. The severity of symptoms was assessed using Sino-nasal outcome test (SNOT-22) and NP recurrence and recorded pre- and postintervention.Results: The findings indicated a higher mean change of SNOT-22 in the case group compared to that of the control group (36.03 ± 10.71 vs. 29.90 ± 11.99; P = 0.041). Moreover, the percentage of NP recurrence in cases was lower than controls; so that receiving VD supplementation has significantly reduced the chance of NP recurrence (odd ratio [95% confidence interval]: 0.298 [0.099-0.900]; P = 0.032).Conclusion: According to the result of the study, the administration of VD supplementation after endoscopic sinus surgery can reduce the severity of CRSwNP symptoms and NP recurrence significantly.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"29"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/aa/72/ABR-12-29.PMC10086644.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9360026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary and Sleep Quality Association in Hemodialysis Patients. 血液透析患者饮食与睡眠质量的关系。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_244_22

Mojgan Mortazavi, Mohammad Hossein Rouhani, Shiva Seirafian, Reyhane Motamedifard, Mohsen Hosseini

{"title":"Dietary and Sleep Quality Association in Hemodialysis Patients.","authors":"Mojgan Mortazavi, Mohammad Hossein Rouhani, Shiva Seirafian, Reyhane Motamedifard, Mohsen Hosseini","doi":"10.4103/abr.abr_244_22","DOIUrl":"https://doi.org/10.4103/abr.abr_244_22","url":null,"abstract":"Background: Poor sleep quality is a common issue among patients with end-stage renal disease (ESRD) who undergo dialysis. Nutritional habits are associated with sleep hygiene in patients undergoing dialysis. The objective of this study was to examine the potential correlation between nutritional status and sleep quality in individuals receiving hemodialysis treatment.Materials and methods: This cross-sectional study included 160 hemodialysis patients. A food frequency questionnaire (FFQ) was used to measure food intake in participants. The Persian-validated version of the Pittsburgh Sleep Quality Index (PSQI) was used to assess sleep quality. Patients were classified as poor or good sleepers with a PSQI score of <5 and >5, respectively.Results: Eighty-four percent of hemodialysis patients had bad sleep hygiene. There was a significant association between sleep quality and educational status and age (P < 0.001). Poor sleepers were older (61.65 years versus 51.12) and less educated (31.1% versus 4%). However, there was no significant difference in the intake of micro- and macronutrients between poor and good sleepers (P > 0.05).Conclusion: The results of this study suggest that sleep quality has no significant relationship with nutrient intake in hemodialysis patients. Demographic factors, such as age and educational status, have played a more effective role than nutritional factors in patients' sleep quality.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"189"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3f/b4/ABR-12-189.PMC10492622.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10589167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Review of Identification Methods for Pathogenic Yeasts: Challenges and Approaches. 病原菌鉴定方法综述:挑战与途径。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_375_22

Hamid Morovati, Mohammad Kord, Kazem Ahmadikia, Saba Eslami, Masoumeh Hemmatzadeh, Kian M Kurdestani, Mohammadreza Khademi, Sima Darabian

{"title":"A Comprehensive Review of Identification Methods for Pathogenic Yeasts: Challenges and Approaches.","authors":"Hamid Morovati, Mohammad Kord, Kazem Ahmadikia, Saba Eslami, Masoumeh Hemmatzadeh, Kian M Kurdestani, Mohammadreza Khademi, Sima Darabian","doi":"10.4103/abr.abr_375_22","DOIUrl":"https://doi.org/10.4103/abr.abr_375_22","url":null,"abstract":"Given the increasing incidence of yeast infections and the presence of drug-resistant isolates, accurate identification of the pathogenic yeasts is essential for the management of yeast infections. In this review, we tried to introduce the routine and novel techniques applied for yeast identification. Laboratory identification methods of pathogenic yeast are classified into three categories; I. conventional methods, including microscopical and culture-base methods II. biochemical/physiological-processes methods III. molecular methods. While conventional and biochemical methods require more precautions and are not specific in some cases, molecular diagnostic methods are the optimum tools for diagnosing pathogenic yeasts in a short time with high accuracy and specificity, and having various methods that cover different purposes, and affordable costs for researchers. Nucleotide sequencing is a reference or gold standard for identifying pathogenic yeasts. Since it is an expensive method, it is not widely used in developing countries. However, novel identification techniques are constantly updated, and we recommend further studies in this field. The results of this study will guide researchers in finding more accurate diagnostic method(s) for their studies in a short period of time.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"187"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c9/55/ABR-12-187.PMC10492613.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10589170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Utilization of Zinc Supplementation Concurrent with Ozone Therapy Ameliorates Diabetic Foot Ulcer and Attenuates Serum Level of C-reactive Protein- A Case Report Study. 治疗利用锌补充与臭氧治疗改善糖尿病足溃疡和降低血清c反应蛋白水平-一个病例报告研究。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_11_22

Reza Dadfar, Layasadat Khorsandi, Reza Goujani, Seyyed Farid Mousavi, Zahra Aslani

引用次数: 1

Examination of the Topical Effect of the Combination of Plantago ovata and Vinegar on the Improvement of Rural Cutaneous Leishmaniasis Lesions. 车前草加醋联合局部治疗农村皮肤利什曼病的疗效观察。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_376_21

Mohammad Ebrahimzadeh Ardakani, Majid Emtiazy, Mohammad Hassan Lotfi, Mehrdad Mousavi

{"title":"Examination of the Topical Effect of the Combination of Plantago ovata and Vinegar on the Improvement of Rural Cutaneous Leishmaniasis Lesions.","authors":"Mohammad Ebrahimzadeh Ardakani, Majid Emtiazy, Mohammad Hassan Lotfi, Mehrdad Mousavi","doi":"10.4103/abr.abr_376_21","DOIUrl":"https://doi.org/10.4103/abr.abr_376_21","url":null,"abstract":"Background: The present study aimed at investigating the topical effect of the combination of Plantago ovata and vinegar on the improvement of rural cutaneous leishmaniasis lesions.Materials and methods: The present randomized double-blind controlled clinical trial was performed on 42 patients with rural skin leishmaniasis. In the case group, in addition to injecting glucantime into the lesion according to the latest national instructions, a combination of P. ovata and vinegar was applied topically twice a day for 8 weeks. In the control group, only glucantime injection into the lesion was performed for 8 weeks according to the latest national guidelines. At the end of the 1st, 2nd, 3rd, 4th, 8th, and 12th weeks after the intervention, the lesion area and improvement were evaluated and recorded.Results: The results of the present study indicated the lesion area in the case group with the mean of 0.35 ± 0.39 cm and 0.18 ± 0.27 cm in the 8th and 12th weeks, respectively was significantly less than that of the control group with the mean of 0.64 ± 0.78 cm and 0.56 ± 0.44, respectively (P < 0.05). Twelve weeks after the intervention, 84.1% of the lesions in the case group and 65.9% of the lesions in the control group were completely improved (P < 0.05).Conclusion: According to the results of the present study, the improvement of leishmaniasis lesion with the topical application of the combination of P. ovata and vinegar was significantly more than that of the control group in the 8th and 12th weeks after the intervention.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"32"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/54/34/ABR-12-32.PMC10086648.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9305335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacterial Superinfection and Antibiotic Management in Patients with COVID-19 Admitted to Intensive Care Medicine in Central Iran: A Follow-Up Study. 伊朗中部重症监护患者的细菌重复感染和抗生素管理:一项随访研究

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_82_22

Zary Nokhodian, Soodabeh Rostami, Parisa Zeraatei, Marzieh Rahimkhorasani, Saeed Abbasi, Somayeh Sadeghi

{"title":"Bacterial Superinfection and Antibiotic Management in Patients with COVID-19 Admitted to Intensive Care Medicine in Central Iran: A Follow-Up Study.","authors":"Zary Nokhodian, Soodabeh Rostami, Parisa Zeraatei, Marzieh Rahimkhorasani, Saeed Abbasi, Somayeh Sadeghi","doi":"10.4103/abr.abr_82_22","DOIUrl":"https://doi.org/10.4103/abr.abr_82_22","url":null,"abstract":"Background: Bacterial superinfections are one of the crucial challenges in patients with coronavirus disease 2019 (COVID-19) that are associated with a high mortality rate. The current study was designed to assess bacterial superinfections and antibiotic management in COVID-19 patients admitted to intensive care unit (ICU).Material and methods: Seventy-three adult intubated patients with COVID-19 were included in a cross-sectional study. The lung aspirate samples were collected in two stages and assessed for bacterial growth by standard methods. Antimicrobial susceptibility testing was performed using the Kirby-Bauer method as recommended by the Clinical Laboratory Standard Institute guideline (2021 edition). Also, demographic and clinical data were collected. The statistical analysis was done by chisquare test and Student's t-test, and a P value <0.05 was considered significant.Results: Forty men and thirty-three women with a mean age of 64.78 ± 13.90 have included in our study. The mean length of hospitalization and stay in ICU were 18.77 ± 12.94 and 13.51 ± 9.83 days, respectively; 84.9% of cases died. Thirty-three patients had a bacterial superinfection mainly caused by Klebsiella spp and Acinetobacter spp; 21.2% of piperacillin/tazobactam consumers' patients survived that; the differences were significant (p = 0.034). A significant relationship was seen between superinfection and length of hospital stay until intubation (p = 0.033).Conclusion: Bacterial superinfection and mortality rates were relatively high in COVID-19 patients admitted to ICU. According to the results, using beta-lactam/beta-lactamase inhibitors antibiotics in hospitalized patients in ICU can effectively control superinfection.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"43"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3f/a1/ABR-12-43.PMC10086667.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9660733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Nasturtium Extract on Oral Cancer. 旱金莲提取物对口腔癌的影响。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_305_21

Amid B Nilash, Jahanfar Jahanbani, Maryam Jolehar

{"title":"Effect of Nasturtium Extract on Oral Cancer.","authors":"Amid B Nilash, Jahanfar Jahanbani, Maryam Jolehar","doi":"10.4103/abr.abr_305_21","DOIUrl":"https://doi.org/10.4103/abr.abr_305_21","url":null,"abstract":"Background: Considering the global prevalence of cancers and the complications of common cancer treatments, there is growing interest in using medicinal herbs to complement cancer treatments and reduce treatment's side effects. Therefore, we investigate the effect of the extract of Nasturtium on the viability of oral cancer cells.Materials and methods: In this experimental study, we prepared aqueous extract from Nasturtium leaves and human oral cancer cells (OCC-24) and normal fibroblast cells (HF2FF cell line) from a cell bank. Then the toxic effect of different concentrations of the extract on cell viability after 24-48 hours of exposure was investigated with the methylthiazol tetrazolium assay. Ultimately, the optical density was measured at 570 nm by an Elisa Reader. Analysis of inhibitory concentration 50 (IC50) was also performed. The data were analyzed by paired Student's t-test and one-way analysis of variance.Results: Data showed that the extract had statistically significant anticancer effects in concentrations above 0.125 mg/ml for 24-hour exposure and in concentrations above 0.5 mg/ml for 48-hour exposure (p-value <0.05). Also, this extract had an adverse effect on the viability of normal cells; however, this effect occurred in high doses of the extract (p-value <0.05). Analysis of IC50 criteria indicates that with increasing time, a higher concentration of the extract is required to inhibit the viability of cancer cells.Conclusion: Because of the results, this aqueous extract can be suggested as a potential therapeutic agent in oral cancer. The best concentration of the extract was found to be 1 mg/ml.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"53"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4e/70/ABR-12-53.PMC10086664.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9660735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0