Annals of 3D printed medicine最新文献_第8页

Computational modeling of probiotic recovery from 3D-bioprinted scaffolds for localized vaginal application 局部阴道应用的3d生物打印支架中益生菌恢复的计算模型

Annals of 3D printed medicine Pub Date : 2023-08-01 DOI: 10.1016/j.stlm.2023.100120

Veeresh Rai , Anthony J. Kyser , Dylan A. Goodin , Mohamed Y. Mahmoud , Jill M. Steinbach-Rankins , Hermann B. Frieboes

{"title":"Computational modeling of probiotic recovery from 3D-bioprinted scaffolds for localized vaginal application","authors":"Veeresh Rai , Anthony J. Kyser , Dylan A. Goodin , Mohamed Y. Mahmoud , Jill M. Steinbach-Rankins , Hermann B. Frieboes","doi":"10.1016/j.stlm.2023.100120","DOIUrl":"10.1016/j.stlm.2023.100120","url":null,"abstract":"<div><p>Lactobacilli, play a beneficial role in the female reproductive tract (FRT), regulating pH via lactic acid metabolism to help maintain a healthy environment. Bacterial vaginosis (BV) is characterized by a dysregulated flora in which anaerobes such as <em>Gardnerella vaginalis</em> (<em>Gardnerella</em>) create a less acidic environment. Current treatment focuses on antibiotic administration, including metronidazole, clindamycin, or tinidazole; however, lack of patient compliance as wel as antibiotic resistance may contribute to 50% recurrence within a year. Recently, locally administered probiotic such as <em>Lactobacillus crispatus</em> (<em>L. crispatus</em>) have been evaluated as a prophylactic against recurrence. To mitigate the lack of patient compliance, sustained probiotic delivery has been proposed via 3D-bioprinted delivery vehicles. Successful delivery depends on a variety of vehicle fabrication parameters influencing timing and rate of probiotic recovery; detailed evaluation of these parameters would benefit from computational modeling complementary to experimental evaluation. This study implements a novel simulation platform to evaluate sustained delivery of probiotics from 3D-bioprinted scaffolds, taking into consideration bacterial lactic acid production and associated pH changes. The results show that the timing and rate of probiotic recovery can be realistically simulated based on fabrication parameters that affect scaffold degradation and probiotic survival. Longer term, the proposed approach could help personalize localized probiotic delivery to the FRT to advance women's health.</p></div>","PeriodicalId":72210,"journal":{"name":"Annals of 3D printed medicine","volume":"11 ","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10424195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10075812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accuracy of 3D printed spine models for pre-surgical planning of complex adolescent idiopathic scoliosis (AIS) in spinal surgeries: a case series 3D打印脊柱模型在脊柱手术中用于复杂青少年特发性脊柱侧凸(AIS)术前规划的准确性:一个病例系列

Annals of 3D printed medicine Pub Date : 2023-08-01 DOI: 10.1016/j.stlm.2023.100117

Abir Dutta , Menaka Singh , Kathryn Kumar , Aida Ribera Navarro , Rodney Santiago , Ruchi Pathak Kaul , Sanganagouda Patil , Deepak M Kalaskar

{"title":"Accuracy of 3D printed spine models for pre-surgical planning of complex adolescent idiopathic scoliosis (AIS) in spinal surgeries: a case series","authors":"Abir Dutta , Menaka Singh , Kathryn Kumar , Aida Ribera Navarro , Rodney Santiago , Ruchi Pathak Kaul , Sanganagouda Patil , Deepak M Kalaskar","doi":"10.1016/j.stlm.2023.100117","DOIUrl":"10.1016/j.stlm.2023.100117","url":null,"abstract":"<div><p>Adolescent idiopathic scoliosis (AIS) is a noticeable spinal deformity in both adult and adolescent population. In majority of the cases, the gold standard of treatment is surgical intervention. Technological advancements in medical imaging and 3D printing have revolutionised the surgical planning and intraoperative decision making for surgeons in spinal surgery. However, its applicability for planning complex spinal surgeries is poorly documented with human subjects. The objective of this study is to evaluate the accuracy of 3D printed models for complex spinal deformities based on Cobb angles between 40° to 95°.This is a retrospective cohort study where, five CT scans of the patients with AIS were segmented and 3D printed for evaluating the accuracy. Consideration was given to the Inter-patient and acquisition apparatus variability of the CT-scan dataset to understand the effect on trueness and accuracy of the developed CAD models. The developed anatomical models were re-scanned for analysing quantitative surface deviation to assess the accuracy of 3D printed spinal models. Results show that the average of the root mean square error (RMSE) between the 3DP models and virtual models developed using CT scan of mean surface deviations for the five 3d printed models was found to be 0.5±0.07 mm. Based on the RMSE, it can be concluded that 3D printing based workflow is accurate enough to be used for presurgical planning for complex adolescent spinal deformities. Image acquisition and post processing parameters, type of 3D printing technology plays key role in acquiring required accuracy for surgical applications.</p></div>","PeriodicalId":72210,"journal":{"name":"Annals of 3D printed medicine","volume":"11 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10427719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10047342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Precision partial scapular bone sarcoma resections and functional reconstructions with 3D printed Patient-Specific implants: Cases report 肩胛骨部分肉瘤的精确切除和3D打印患者专用植入物的功能重建：病例报告

Annals of 3D printed medicine Pub Date : 2023-05-01 DOI: 10.1016/j.stlm.2023.100105

Kwok Chuen Wong, Wang Kei Chiu, Man Lung Moses Li, Hiu Woo Jacky Lau, Shekhar Madhukar Kumta

引用次数: 0

Development of a topologically optimized patient-specific mandibular reconstruction implant for a Brown class II defect 针对Brown II级缺损的拓扑优化患者专用下颌重建植入物的研制

Annals of 3D printed medicine Pub Date : 2023-05-01 DOI: 10.1016/j.stlm.2023.100107

Olivier Schottey , Stijn E.F. Huys , G. Harry van Lenthe , Maurice Y. Mommaerts , Jos Vander Sloten

{"title":"Development of a topologically optimized patient-specific mandibular reconstruction implant for a Brown class II defect","authors":"Olivier Schottey , Stijn E.F. Huys , G. Harry van Lenthe , Maurice Y. Mommaerts , Jos Vander Sloten","doi":"10.1016/j.stlm.2023.100107","DOIUrl":"10.1016/j.stlm.2023.100107","url":null,"abstract":"<div><p>Segmental defects affecting the continuity of the mandible have a profound impact on a patient's quality of life. Adequate reconstruction of such continuity defects is essential to restore aesthetics and function. While reconstruction using an autologous bone transplant supported by a stock reconstruction plate is the gold standard treatment, it has various drawbacks that can be attested to the plates’ off-the-shelf nature.</p><p>To mitigate these drawbacks, this study develops a patient-specific implant for the reconstruction of Brown class II defects with a high ramal osteotomy. The implant is intended to be additively manufactured in Ti6Al4V grade 23 ELI and features porous scaffold zones at the symphyseal and condylar sides which can induce bone ingrowth.</p><p>Finite element (FE) analyses were used to assess the implants’ performance in terms of failure, stability and stress shielding by simulating four clenching tasks. In addition, the implant was topologically optimized and re-evaluated.</p><p>The results showed that the implant experienced stress below its yield strength and fatigue limit. Relative micromotions between the implant and the bone indicated adequate stability to allow bone ingrowth to occur. Strains in the bone indicated limited stress shielding should occur between screw connections and around the osteotomy planes.</p><p>Finally, topological optimization reduced implant volume by 49% compared to the initial design, while FE analyses showed similar performance to the original design. The resulting implant is a promising first prototype that is numerically evaluated and can be optimized further in terms of fixation, surgical approach and dental restoration by in situ testing.</p></div>","PeriodicalId":72210,"journal":{"name":"Annals of 3D printed medicine","volume":"10 ","pages":"Article 100107"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44938359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

3D printed biosensor for continuous glucose measurement in cell cultures 用于细胞培养中连续葡萄糖测量的3D打印生物传感器

Annals of 3D printed medicine Pub Date : 2023-05-01 DOI: 10.1016/j.stlm.2023.100111

Nenad Krstić , Jens Jüttner , Lars Giegerich , Margot Mayer , Monika Knuth , Achim Müller , Christiane Thielemann

{"title":"3D printed biosensor for continuous glucose measurement in cell cultures","authors":"Nenad Krstić , Jens Jüttner , Lars Giegerich , Margot Mayer , Monika Knuth , Achim Müller , Christiane Thielemann","doi":"10.1016/j.stlm.2023.100111","DOIUrl":"10.1016/j.stlm.2023.100111","url":null,"abstract":"<div><p>A novel 3D-printed glucose sensor is presented for cell culture application. Glucose sensing was performed using a fluorescence resonance energy transfer (FRET)-based assay principle based on ConA and dextran. Both molecules are encapsulated in alginate microspheres and embedded in the UV-curable, stable hydrogel polyvinyl alcohol (PVA). The rheology of the formulation was adapted to obtain good properties for an extrusion-based printing process. The printed sensor structures were tested for their ability to detect glucose <em>in vitro</em>. A proportional increase in fluorescence intensity was observed in a concentration range of 0 - 2 g/L glucose. Tests with HEK cell cultures also showed good cell compatibility and excellent adhesion properties on plasma-treated Petri dishes. The printed sensors were able to detect the glucose decay associated with the metabolic activities of the fast-growing HEK cells in the cell culture medium over ten days. The proof-of-principle study shows that metabolic processes in cell cultures can be monitored with the new printed sensor using a standard fluorescence wide-field microscope.</p></div>","PeriodicalId":72210,"journal":{"name":"Annals of 3D printed medicine","volume":"10 ","pages":"Article 100111"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49665423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High-fidelity simulation training for nasal bridle placement with a 3D printed model 高保真仿真训练与3D打印模型的鼻笼头安置

Annals of 3D printed medicine Pub Date : 2023-05-01 DOI: 10.1016/j.stlm.2023.100108

Yu-Hui Huang MD, MS , David Nascene MD , Benjamin Spilseth MD, MBA , Jeffrey A. Chuy MD

{"title":"High-fidelity simulation training for nasal bridle placement with a 3D printed model","authors":"Yu-Hui Huang MD, MS , David Nascene MD , Benjamin Spilseth MD, MBA , Jeffrey A. Chuy MD","doi":"10.1016/j.stlm.2023.100108","DOIUrl":"10.1016/j.stlm.2023.100108","url":null,"abstract":"<div><h3>Background</h3><p>Nasal bridles help prevent nasoenteric feeding tube dislodgement. If placed incorrectly, nasal bridles can cause injury, epistaxis, skin ulceration, or failure to prevent dislodgment. Training is frequently performed on patients, which can lead to discomfort or complications. To improve training prior to placing nasal bridles in patients, we utilized an anatomically accurate 3D printed simulator for nasal bridle placement training.</p></div><div><h3>Methods</h3><p>The model was modified from a nasopharyngeal swab simulator by BONE 3D, which was developed from imaging data through segmentation, computer-aided design, and 3D printing. Eighteen radiology residents and 3 medical students received a pre-test covering the anatomical knowledge and technique relevant to nasal bridle placement followed by a training session using the model. After training, participants provided feedback on the impact of training with the model on anatomical knowledge, hands-on skills, and confidence <em>via</em> a post-test using a 5-point Likert scale [from 1 (not beneficial/confident) to 5 (extremely beneficial/ confident)].</p></div><div><h3>Results</h3><p>Twenty participants completed both pre- and post-tests. The group performed significantly better on the post-test (4.8 ± 0.52) than the pre-test (2.6 ± 1.64), and the intervention demonstrated a large effect on knowledge (<em>p</em> < 0.0001; <em>d</em> = 1.82) and confidence level (<em>p</em> < 0.0001, <em>d</em> = 2.45) with mean magnitude of improvement of 2.3 out of 5 points. All respondents requested the 3D printed model be offered in formal training.</p></div><div><h3>Conclusions</h3><p>An anatomically accurate 3D printed model is a feasible and acceptable training aid with the potential to facilitate novice knowledge, proficiency, and confidence for nasal bridle placement.</p></div>","PeriodicalId":72210,"journal":{"name":"Annals of 3D printed medicine","volume":"10 ","pages":"Article 100108"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48894226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-house virtual surgical planning for mandibular reconstruction with fibula free flap: Case series and literature review 腓骨游离皮瓣重建下颌骨的室内虚拟手术计划：病例系列和文献综述

Annals of 3D printed medicine Pub Date : 2023-05-01 DOI: 10.1016/j.stlm.2023.100109

Steve Maisi , Mauro Dominguez , Peta Charmaine Gilong , Chung Tze Kiong , Syarfa Hajam , Ahmad Fadhli Ahmad Badruddin , Han Fong Siew , Saravanan Gopalan , Kok Tuck Choon

{"title":"In-house virtual surgical planning for mandibular reconstruction with fibula free flap: Case series and literature review","authors":"Steve Maisi , Mauro Dominguez , Peta Charmaine Gilong , Chung Tze Kiong , Syarfa Hajam , Ahmad Fadhli Ahmad Badruddin , Han Fong Siew , Saravanan Gopalan , Kok Tuck Choon","doi":"10.1016/j.stlm.2023.100109","DOIUrl":"10.1016/j.stlm.2023.100109","url":null,"abstract":"<div><p>The fibula free flap (FFF) surgery has long been used for complicated mandibular reconstruction. Virtual surgical planning (VSP) has been incorporated into the reconstruction planning by surgeons and has been found to reduce operating time and surgeon stress intraoperatively. When compared to traditional reconstruction, VSP enhances accuracy, surgical efficiency, and clinical outcomes. However, VSP on the other hand, need advanced technology such as software and 3D printing equipment, which are not always accessible in all centres. We describe our workflow on VSP reconstruction of the mandible with FFF by using open-source software.</p></div><div><h3>Methods</h3><p>Three patients underwent mandible reconstruction with FFF. VSP was used for all reconstruction planning.</p></div><div><h3>Results</h3><p>The mean operative time was min 765 minutes (range: 615 – 960 minutes), the mean ischemic time was 260 minutes (range: 120 – 355 minutes) and the mean length of stay was 10.7 days (range: 10 – 12 days). There were no flap failures. There were no major complications.</p></div><div><h3>Conclusion</h3><p>VSP is a very viable method that saves time and cost, making surgery more efficient.</p></div>","PeriodicalId":72210,"journal":{"name":"Annals of 3D printed medicine","volume":"10 ","pages":"Article 100109"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48151387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of additive manufacturing in the biomedical field- A review 增材制造技术在生物医学领域的应用综述

Annals of 3D printed medicine Pub Date : 2023-05-01 DOI: 10.1016/j.stlm.2023.100110

A. Harding , A. Pramanik , A.K. Basak , C. Prakash , S. Shankar

引用次数: 2

Novel technique of post mortem cranial bone reconstruction using 3D printed skull clips: Technical note 使用3D打印颅骨夹进行死后颅骨重建的新技术：技术说明

Annals of 3D printed medicine Pub Date : 2023-05-01 DOI: 10.1016/j.stlm.2023.100104

L Bilton , S B Harvey , B I'Ons , H Green

引用次数: 0

Are 3D printed resin surgical splints safe to use in the mouth? A comparative in vitro study 3D打印树脂外科夹板在口腔中使用安全吗?一项体外比较研究

Annals of 3D printed medicine Pub Date : 2023-02-01 DOI: 10.1016/j.stlm.2022.100097

Yang Sha , Bernadette Quah , Timothy Jie Han Sng , Chee Weng Yong , Intekhab Islam , Raymond Chung Wen Wong

{"title":"Are 3D printed resin surgical splints safe to use in the mouth? A comparative in vitro study","authors":"Yang Sha , Bernadette Quah , Timothy Jie Han Sng , Chee Weng Yong , Intekhab Islam , Raymond Chung Wen Wong","doi":"10.1016/j.stlm.2022.100097","DOIUrl":"https://doi.org/10.1016/j.stlm.2022.100097","url":null,"abstract":"<div><h3>Introduction</h3><p>In orthognathic surgery, surgical splints made of polymethymethacrylate or its variants are used to position the jaws before osteofixation, and occasionally left in-situ for a short duration to guide the occlusion postoperatively. However, allergic reactions can be caused by unreacted dental resin (monomers). Computer aided design/Computer Aided Manufacturing (CADCAM) surgical splints are now more commonly used than the conventionally “handmade” splints. It is unclear if these splints contain uncured residual monomers in any detectable quantity.</p></div><div><h3>Materials and methods</h3><p>CADCAM surgical splints were made from 2 commonly used resins and compared with splints made manually with autopolymerising resin. The splints were immersed in saline at 37°C for 2 weeks to simulate use in the mouth, and the contents were analysed for uncured resin leaching with High Power Liquid Chromatography (HPLC) at different time intervals.</p></div><div><h3>Results</h3><p>Uncured resin content was higher in the conventionally made splint (15,246µg/L on Day 1, 13,539 µg/L on Day 7, and 6,203µg/L on Day 14). One of the CADCAM resins had undetectable monomer quantities, while the other CADCAM resin demonstrated significantly less free monomer compared to the autopolymerising resin (382 µg/L on Day 1, 179 µg/L on Day 7 and 65.2 µg/L on Day 14).</p></div><div><h3>Conclusion</h3><p>CADCAM surgical splints have much lower free monomer content compared to the conventionally made splint, and are safe for clinical use.</p></div>","PeriodicalId":72210,"journal":{"name":"Annals of 3D printed medicine","volume":"9 ","pages":"Article 100097"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49714335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0