Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe最新文献

{"title":"Evaluation of leucomethylene blue as a probe for assessing antioxidant activity reveals a potential application in the assessment of male fertility","authors":"Robert J. Aitken ,&nbsp;Alex Wilkins ,&nbsp;Natasha Harrison ,&nbsp;Aleona Swegen ,&nbsp;Sarah Lambourne","doi":"10.1016/j.arres.2025.100121","DOIUrl":"10.1016/j.arres.2025.100121","url":null,"abstract":"<div><div>The oxidation of leucomethylene blue (LMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) are colorigenic reactions, generating methylene blue (<em>M</em>⁺) and the ABTS^•+ cation radical, respectively. In this study, we have analyzed the conditions under which these probes become oxidized and explored their application in the assessment of antioxidant activity. Using hematin as a catalyst, LMB responded to hydrogen peroxide and cumene hydroperoxide, whereas ABTS could only respond to the latter. However, in the presence of horse radish peroxidase, both probes responded specifically and dose-dependently to hydrogen peroxide. ABTS and LMB could also be oxidized in the anodic chamber of an electrochemical cell, permitting both pre-activation assays of free radical formation and post-activation assessments of free radical scavenging activity. In the pre-activation mode, both ABTS and LMB successfully revealed DMSO's capacity to inhibit free radical formation, in contrast to the ‘green’ solvent Cyrene™, which was virtually devoid of such activity. In the post-activation mode, the LMB oxidation product, methylene blue, was shown to be particularly sensitive to the 2-electron reducing properties of vitamin C. In contrast, ABTS responded more sensitively to compounds, like resveratrol, that used hydrogen atom transfer and one electron reduction to achieve their antioxidant action. Only LMB-based antioxidant assessments correlated with sperm motility (<em>P</em> &lt; 0.001), suggesting this probe's sensitivity to 2-electron reduction may find particular application in diagnostic andrology. Evidently, not all redox sensors are created equal, so in future assessments of antioxidant activity, it will be important to match the chemistry of the probe with its analytical application.</div></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"14 ","pages":"Article 100121"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox Metabolomics of Menthol in Children's Plasma with Second-Hand Cigarette and Electronic Cigarette Exposures","authors":"Matthew Ryan Smith ,&nbsp;Zachery R. Jarrell ,&nbsp;Ken H Liu ,&nbsp;Choon-Myung Lee ,&nbsp;Edward T Morgan ,&nbsp;Young-Mi Go ,&nbsp;Dean P. Jones","doi":"10.1016/j.arres.2025.100122","DOIUrl":"10.1016/j.arres.2025.100122","url":null,"abstract":"<div><h3>Background</h3><div>Cigarettes and electronic cigarettes generate many redox-active materials which could impact children's health through second-hand exposures. High-resolution metabolomics methods enable use of non-targeted mass spectrometry of plasma to test for redox consequences of second-hand exposures.</div></div><div><h3>Objectives</h3><div>Our objectives were to test for oxidative stress metabolites and altered metabolic pathways associated with second-hand exposure to redox-active flavorants and flavorant metabolites in plasma of infants and children.</div></div><div><h3>Methods</h3><div>Untargeted plasma metabolomics data for infants and children in a population known to include individuals with second-hand exposures to cigarettes and electronic cigarettes were analyzed for cotinine and metabolites of flavorants. A metabolome-wide association study (MWAS) was performed separately for cotinine and menthol glucuronide, derived from the redox-active flavorant, menthol. Pathway enrichment analysis was used to identify metabolic pathways, and xMWAS was used to detect metabolic communities associated with flavorant metabolites.</div></div><div><h3>Results</h3><div>Menthol glucuronide was one of several flavorant metabolites positively correlated with cotinine. MWAS and pathway enrichment analysis revealed that some pathways associated with both menthol glucuronide and cotinine, while others only associated with menthol glucuronide, including sphingolipid, glycerophospholipid, antioxidant, N-glycan and mitochondrial energy metabolism. 4-hydroxynonenal and other oxidized lipids positively correlated with menthol glucuronide.</div></div><div><h3>Discussion</h3><div>The results show that flavorants from second-hand electronic cigarette and cigarette exposures in infants and children are associated with changes in redox metabolism which are known to associate with human lung diseases.</div></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"14 ","pages":"Article 100122"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NRF2 protects lung epithelial cells from wood smoke particle toxicity","authors":"Sarah E. Lacher ,&nbsp;Tessa Schumann ,&nbsp;Ryan Peters ,&nbsp;Christopher Migliaccio ,&nbsp;Andrij Holian ,&nbsp;Matthew Slattery","doi":"10.1016/j.arres.2024.100115","DOIUrl":"10.1016/j.arres.2024.100115","url":null,"abstract":"<div><div>Wildfire smoke is a potential source of oxidative stress in lung epithelial tissue. The response to oxidative stress is controlled by the transcription factor NRF2, which is the central regulator of antioxidant gene expression. If wood smoke particle (WSP) exposure induces reactive oxygen species (ROS) in epithelial cells, then NRF2 may protect against pathological conditions resulting from increased oxidative stress through changes in gene expression. We used two lung epithelial cell lines to test this hypothesis <em>in vitro</em>: A549, which harbor a mutation resulting in constitutive activation of NRF2, and BEAS-2B, which show limited NRF2 activity under basal conditions, but high inducibility during oxidative stress. In BEAS-2B cells, WSP exposure leads to increased cellular ROS, activation of NRF2, and upregulation of the NRF2 target genes <em>NQO1, GCLM,</em> and <em>SRXN1</em>. WSP exposure also increased ROS in A549 cells, although NRF2 activation and antioxidant gene upregulation were less robust as both were basally high in this cell line. Overall, the degree of ROS induction by WSP across cell lines is dependent upon NRF2 activity, and a similar pattern was observed for WSP cytotoxicity. WSP also sensitized both cell lines to the ferroptosis inducer erastin in a manner that is correlated with NRF2 activity. Knockout of <em>NRF2</em> in A549 resulted in higher WSP-induced ROS generation, cytotoxicity, and erastin sensitivity. Taken together, these results suggest that NRF2 serves as a protective factor against wood smoke induced ROS and oxidative stress in lung epithelial cells.</div></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"13 ","pages":"Article 100115"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piceatannol reduces radiation-induced DNA double-strand breaks by suppressing superoxide production and enhancing ATM-dependent repair efficiency","authors":"Tomoya Suzuki ,&nbsp;Ryoya Tetsuka ,&nbsp;Atsuya Iwasaki ,&nbsp;Tsutomu Shimura ,&nbsp;Ryoichi Hirayama ,&nbsp;Asako J Nakamura","doi":"10.1016/j.arres.2024.100114","DOIUrl":"10.1016/j.arres.2024.100114","url":null,"abstract":"<div><p>In contemporary society, humans are susceptible to various radiation-borne hazards, including exposure to therapeutic modalities using low-linear energy transfer (low-LET) radiations (X-rays and γ-rays), natural high-LET radiation sourced from cosmic rays, as well as nuclear accidents such as the Fukushima Daiichi Nuclear Power Plant incident. Therefore, this threat incites an imminent necessity to develop novel radioprotective agents against a wide range of LET radiation and elucidate the underlying molecular mechanisms. This study aimed at assessing the radioprotectivity of Piceatannol (PIC), a potent antioxidant polyphenol present in abundance in passion fruit, by investigating its effects on radiation-induced reactive oxygen species (ROS) production and the consequent DNA double-strand break (DSB) capacity and cellular senescence. Specifically, total ROS was evaluated by DCFDA staining, mitochondrial superoxide by MitoSOX staining, DSB by γ-H2AX immunostaining, and cellular senescence by Senescence-associated-β-galactosidase staining.</p><p>The results demonstrated that PIC administration prior to exposure to both X-ray and high-LET radiation impeded radiation-induced DSB by suppressing ROS production. Interestingly, post-irradiation PIC treatment did not alter ROS levels but enhanced the efficiency of Ataxia Telangiectasia Mutated (ATM)-mediated DSB repair. Additionally, post-irradiation PIC treatment diminished senescence-associated beta-galactosidase levels, indicating that it hinders cellular senescence.</p><p>Conclusively, PIC exerts radioprotective effects against a wide range of LET radiation. The study findings validate the potential application of PIC not only as a radical scavenger but also as a novel DSB repair-activating radioprotective agent.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"13 ","pages":"Article 100114"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137924000213/pdfft?md5=b11373d91805a1cf814a18ae661dec23&pid=1-s2.0-S2667137924000213-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring oxysterols and protein carbonylation in cervicovaginal secretions as biomarkers for cervical cancer development","authors":"Busra Kose ,&nbsp;Serkan Erkanlı ,&nbsp;Alper Koçak ,&nbsp;Coskun Guzel ,&nbsp;Theo Luider ,&nbsp;Irundika H.K. Dias ,&nbsp;Ahmet Tarik Baykal","doi":"10.1016/j.arres.2024.100111","DOIUrl":"10.1016/j.arres.2024.100111","url":null,"abstract":"<div><p>Cervical cancer, a major global health issue and the fourth most common cancer among women, is strongly linked to Human Papillomavirus (HPV) infection. Emerging evidence indicates that oxidative stress plays a critical role in the carcinogenesis of cervical tissue. This study investigates the relationship between oxidative stress markers—specifically oxysterols, lipid oxidation, and protein carbonylation—and the progression of cervical neoplasia.</p><p>Oxysterols, which are elevated in various inflammatory diseases and cancers, were measured in cervicovaginal fluid samples using LC-MS/MS. The targeted oxysterols included 27-hydroxycholesterol (27-OHC), 7β-hydroxycholesterol (7β-OHC), 7-ketocholesterol (7-KC), and 7α,27-dihydroxycholesterol (7α,27-diOHC). Among these, 7α,27-dihydroxycholesterol was significantly increased in correlation with the severity of neoplastic stages. In parallel, protein carbonylation, an indicator of cellular oxidative stress, was assessed. Results revealed higher levels of protein carbonylation in neoplastic samples compared to non-neoplastic controls. These modifications were further analysed through redox proteomics to identify the specific proteins affected.</p><p>The study demonstrates that elevated lipid oxidation and protein carbonylation in cervicovaginal secretions are linked to the development and progression of cervical cancer. Identifying these biomarkers may improve screening strategies, enabling the identification of individuals at increased risk for cervical neoplasia and guiding timely interventions.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"13 ","pages":"Article 100111"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137924000183/pdfft?md5=9aa608a17eb8ee499fd581b8b30174ce&pid=1-s2.0-S2667137924000183-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absence of mitochondrial CX9C-CX10C protein Cox12 generates oxidative and nitrosative stress in Saccharomyces cerevisiae: Implication on cellular redox homeostasis","authors":"Soumyajit Mukherjee ,&nbsp;Shubhojit Das ,&nbsp;Sourav Kumar Patra ,&nbsp;Mayukh Das ,&nbsp;Sanjay Ghosh ,&nbsp;Alok Ghosh","doi":"10.1016/j.arres.2024.100112","DOIUrl":"10.1016/j.arres.2024.100112","url":null,"abstract":"<div><p>Mitochondrial intermembrane space (IMS) harbors a series of small, evolutionarily conserved redox-active cysteine-rich proteins. These proteins are essential for the functioning of cytochrome c oxidase, but their role in maintaining cellular redox processes is unknown. Here, we find out that in the absence of two such cysteine-rich Cx₉C-Cx₁₀C proteins, cytochrome c oxidase subunit 12 (Cox12) or cytochrome c oxidase assembly factor 6 (Coa6), <em>Saccharomyces cerevisiae</em> cells become sensitive under the oxidative and nitrosative stress. Interestingly, knockout of <em>COX12</em> generates a significant amount of endogenous reactive oxygen species (ROS) and reactive nitrogen species (RNS) as evidenced by FACS analysis. Moreover, cellular redox status, redox-active enzymes glutathione reductase, catalase, S-nitroso glutathione reductase, and protein nitration were significantly affected in Cox12 null cells. Further, we found that an overexpression of <em>COX12</em> partially protects mitochondrial respiratory subunit Sdh2 under oxidative and nitrosative stress. Taken together, we provide proof of evidence that cysteine-rich proteins in the IMS dynamically control the cellular redox milieu and actively prevent reactive nitrogen and oxygen species generation.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"13 ","pages":"Article 100112"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137924000195/pdfft?md5=f795c7f807f8d09ef7d402872b728a55&pid=1-s2.0-S2667137924000195-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical-mediated modulation of signaling pathways: A promising avenue for drug discovery","authors":"Jibon Kumar Paul ,&nbsp;Mahir Azmal ,&nbsp;ANM Shah Newaz Been Haque ,&nbsp;Omar Faruk Talukder ,&nbsp;Meghla Meem ,&nbsp;Ajit Ghosh","doi":"10.1016/j.arres.2024.100113","DOIUrl":"10.1016/j.arres.2024.100113","url":null,"abstract":"<div><p>Phytochemicals are bioactive compounds derived from plants, renowned for their therapeutic potential. This study explores their pivotal roles in therapy and drug development, focusing on their interactions with crucial Signaling pathways and biological processes. Phytochemicals such as curcumin from turmeric, resveratrol from grapes, and epigallocatechin gallate (EGCG) from green tea, exert their effects primarily by modulating key pathways like PI3K/AKT, MAPK-ERK, Wnt, and Hedgehog, which are integral to cellular functions and disease progression. Phytochemicals like curcumin demonstrate potent anticancer activities by inhibiting proteins involved in cell proliferation, inducing apoptosis, and suppressing angiogenesis. Phytochemicals such as resveratrol also exhibit anti-inflammatory properties by targeting cytokine production and oxidative stress pathways, thereby alleviating chronic inflammatory conditions implicated in various diseases. Their (eg. EGCG's) antioxidant capabilities help neutralize free radicals and reduce oxidative damage, contributing to cellular health and longevity. Moreover, phytochemicals play a pivotal role in metabolic disorders such as diabetes and obesity by regulating glucose metabolism, lipid profiles, and insulin sensitivity. They also show promise in neurodegenerative diseases by protecting neuronal cells, enhancing cognitive functions, and potentially mitigating neuroinflammation and oxidative stress. For instance, berberine, an alkaloid found in several plants, shows promise in regulating glucose levels and improving insulin sensitivity. Additionally, ginsenosides from ginseng are known to protect neuronal cells, enhance cognitive functions, and potentially mitigate neuroinflammation and oxidative stress in neurodegenerative diseases. Despite their promising therapeutic benefits, challenges remain, particularly in ensuring bioavailability and standardization of phytochemical extracts for clinical use. Advances in screening techniques and formulation strategies aim to enhance their efficacy and integration into modern therapeutic approaches. Their multifaceted roles in modulating cellular processes underscore their importance as potential alternatives or complements to traditional pharmacotherapies, driving innovation in drug development and personalized medicine.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"13 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137924000201/pdfft?md5=73e609cfc573df68e79c3b632927c8d7&pid=1-s2.0-S2667137924000201-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142149240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin protects against chromium induced oxidative stress-mediated changes in energy metabolism of rat hepatic, cardiac and renal tissues","authors":"Priyanka Ghosh ,&nbsp;Madhuri Datta ,&nbsp;Romit Majumder ,&nbsp;Aindrila Chattopadhyay ,&nbsp;Debasish Bandyopadhyay","doi":"10.1016/j.arres.2024.100110","DOIUrl":"10.1016/j.arres.2024.100110","url":null,"abstract":"<div><p>Chromium (Cr) is one of the most prevalent and potentially hazardous heavy metal found in the environment that can cause carcinogenic, genotoxic, and organ-specific irreversible complications. The most severe adverse outcome of Cr on humans involves oxidative stress. Melatonin was evidenced to alleviate such stress with various mechanisms including antioxidative potential and metal chelation. Male Wistar rats were divided into 4 groups and treated for 14 days. The first group (control) was treated with vehicle; the second group was orally administered with melatonin (20 mg/kg b.w./day); the third group was injected with sodium dichromate dihydrate (5 mg/kg bw, s.c. every alternate day); and the fourth group was administered with melatonin, 30 min before Cr administration. The treatment of rats with Cr (VI) was found to affect the metabolic pathways by altering the activities of enzymes possibly through uncompetitively binding with them. The current study also demonstrated that melatonin efficiently preserved the glucose levels and blood lipid profile. Moreover, melatonin was further found to protect the activities of glycolytic, Krebs’ cycle, and ETC enzymes. Further, melatonin pre-treatment reduced the production of <span><math><msubsup><mi>O</mi><mn>2</mn><mrow><mo>.</mo><mo>−</mo></mrow></msubsup></math></span> anion free radical and Ca²⁺ overload to protect the mitochondria at the ultrastructural level and reduced DNA damage to some extent. Therefore, this research strongly recommends melatonin as a therapeutic molecule against Cr-induced oxidative stress-mediated liver, heart, and kidney disorders.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"12 ","pages":"Article 100110"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137924000171/pdfft?md5=725dea399f998c3d9db75e3817f84396&pid=1-s2.0-S2667137924000171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141844627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum oxidative stress levels and antioxidant capacity in prediabetes","authors":"Buse Balci ,&nbsp;Buket Kin Tekce ,&nbsp;Gulali Aktas","doi":"10.1016/j.arres.2024.100106","DOIUrl":"10.1016/j.arres.2024.100106","url":null,"abstract":"<div><p>Prediabetes is a metabolic disorder marked by blood sugar levels that are elevated than usual but not yet high enough to be classified as type 2 diabetes. It is known that raised oxidative stress and insufficient antioxidant status play a role in the pathogenesis of type 1 and type 2 diabetes. In this study, we aimed to measure total oxidative stress and antioxidant status in prediabetic patients and compare them with healthy volunteers. Subjects with prediabetes according to their HbA1c and blood sugar levels in their routine tests were included in the study. The control group consisted of healthy volunteers who visited our clinics for routine health screening and had no health problems<strong><em>.</em></strong> TAS and TOS levels of the groups were compared. Mean TAS and median TOS values ​​were significantly different among study and control groups (<em>p</em> &lt; 0.001 for both). Blood TOS level was a reliable risk factor of prediabetes, taking into account TAS, weight, triglycerides, and GFR. Higher oxidative stress and lower antioxidant levels were found in prediabetic patients compared to healthy ones. Diabetes development and related complications can be prevented by interventions for these markers in serum.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"12 ","pages":"Article 100106"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137924000134/pdfft?md5=ee49f38d658cbb481ec81689cdf33f65&pid=1-s2.0-S2667137924000134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141623062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondria-targeted antioxidant, mitoQ protects hepatic tissue from N-nitrosodiethylamine-induced damage by modulating mitochondrial function and redox status","authors":"H.S. Qsee ,&nbsp;Sachin Shetty ,&nbsp;Shounak De ,&nbsp;Sanjay Bharati","doi":"10.1016/j.arres.2024.100108","DOIUrl":"10.1016/j.arres.2024.100108","url":null,"abstract":"<div><h3>Background</h3><p>Targeting mitochondrial oxidative stress can be a promising strategy for the prevention of hepatocellular carcinoma (HCC). In the current study, we investigated the modulatory effect of mitochondria-targeted antioxidant, mitoQ against N-<em>nitrosodiethylamine</em> (NDEA)-induced hepatic damage in mouse.</p></div><div><h3>Methods</h3><p>BALB/c mice were administered NDEA (10 mg/kg b. w., single dose, intraperitoneally) and the hepatoprotective effect of mitoQ was studied by administering mitoQ (0.125 mg/kg b. w., orally once a week) to the animals. The administration of mitoQ started two weeks prior the NDEA administration. The animals were sacrificed 24 h following NDEA administration after which the blood samples and hepatic tissues were collected. Serum was used for the estimation of liver injury markers and hepatic tissues were analyzed for histopathological changes, antioxidant defense status, mitochondrial functional status, level of mitochondrial reactive oxygen species (mtROS) and mitochondrial lipid peroxidation (mtLPO).</p></div><div><h3>Results</h3><p>MitoQ treatment to the NDEA-challenged group normalized liver injury markers, level of mtROS and mtLPO. MitoQ treatment also improved the status of mitochondrial antioxidant defense system, mitochondrial complex enzymes.</p></div><div><h3>Conclusion</h3><p>Our findings indicate that mito-Q significantly protected against NDEA-induced hepatic damage by modulating mitochondrial function and redox status which may be one of the causes of its purported chemopreventive effect.</p></div>","PeriodicalId":72106,"journal":{"name":"Advances in redox research : an official journal of the Society for Redox Biology and Medicine and the Society for Free Radical Research-Europe","volume":"12 ","pages":"Article 100108"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667137924000158/pdfft?md5=33de7d64c50e6f17273a128f14564a1b&pid=1-s2.0-S2667137924000158-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141959703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}