Advances in cancer biology - metastasis最新文献

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An insight of various vesicular systems, erythrosomes, and exosomes to control metastasis and cancer 深入了解各种囊泡系统、红细胞体和外泌体,以控制转移和癌症
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2023.100103
Mrunali Patil , Afzal Hussain , Mohammad A. Altamimi , Sumel Ashique , Nazima Haider , Abdul Faruk , Tahir Khuroo , Abdulla Sherikar , Mohd Usman Mohd Siddique , Azim Ansari , Tabassum Khair Barbhuiya
{"title":"An insight of various vesicular systems, erythrosomes, and exosomes to control metastasis and cancer","authors":"Mrunali Patil ,&nbsp;Afzal Hussain ,&nbsp;Mohammad A. Altamimi ,&nbsp;Sumel Ashique ,&nbsp;Nazima Haider ,&nbsp;Abdul Faruk ,&nbsp;Tahir Khuroo ,&nbsp;Abdulla Sherikar ,&nbsp;Mohd Usman Mohd Siddique ,&nbsp;Azim Ansari ,&nbsp;Tabassum Khair Barbhuiya","doi":"10.1016/j.adcanc.2023.100103","DOIUrl":"10.1016/j.adcanc.2023.100103","url":null,"abstract":"<div><p>Cancer is the most challenging global health issue despite advancement in new drug development and biological understanding of oncology. Metastasis is the hallmark of cancer development resulting in huge death and it remained poorly understood. Various research articles published to control cancer and metastasis using lipidic nanocarriers so far. However, safety and high patient compliance are the prime concern. Medication is always of major concern for a clinician before prescribing any dosage form or drug. The high toxicity profile of anticancer drugs led to increased financial burden of treatment, mortality and complex consequences. Therefore, conventional dosage forms failed to culminate various issues related to therapeutic efficacy and drug related toxicity. Then, many researchers tried the novel drug delivery systems for effective and safe targeting of anticancer drugs. Exosomes, vesicular systems, and erythrocytes have been explored to treat cancer. It was imperative to compile major findings from these researches carried out so far. In the review, we highlighted application of exosomes, liposomes, and erythrocytes to control metastasis and cancer. Moreover, we have addressed various critical attributes of liposomes while formulation design for improved therapeutic efficacy and mechanistic perspective for drug targeting using the described vesicular carriers. Finally, we compiled major findings of clinical data published in clinical research plate-form. This review highlighted the major findings associated with nanovesicles based tumor targeting in which anticancer drugs are encapsulated in suitable vesicular systems and reach to site specific delivery of drugs.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100103"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48811351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Benzophenone-3 exposure alters composition of tumor infiltrating immune cells and increases lung seeding of 4T1 breast cancer cells 二苯甲酮-3暴露改变肿瘤浸润免疫细胞的组成,增加4T1乳腺癌细胞的肺播种
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2022.100080
Stephanie M. Morin , Kelly J. Gregory , Brenda Medeiros , Tigist Terefe , Reyhane Hoshyar , Ahmed Alhusseiny , Shiuan Chen , Richard C. Schwartz , D. Joseph Jerry , Laura N. Vandenberg , Sallie S. Schneider
{"title":"Benzophenone-3 exposure alters composition of tumor infiltrating immune cells and increases lung seeding of 4T1 breast cancer cells","authors":"Stephanie M. Morin ,&nbsp;Kelly J. Gregory ,&nbsp;Brenda Medeiros ,&nbsp;Tigist Terefe ,&nbsp;Reyhane Hoshyar ,&nbsp;Ahmed Alhusseiny ,&nbsp;Shiuan Chen ,&nbsp;Richard C. Schwartz ,&nbsp;D. Joseph Jerry ,&nbsp;Laura N. Vandenberg ,&nbsp;Sallie S. Schneider","doi":"10.1016/j.adcanc.2022.100080","DOIUrl":"10.1016/j.adcanc.2022.100080","url":null,"abstract":"<div><p>Environmental chemicals are a persistent and pervasive part of everyday life. A subset of environmental chemicals are xenoestrogens, compounds that bind to the estrogen receptor (ER) and drive estrogen-related processes. One such chemical, benzophenone-3 (BP3), is a common chemical in sunscreen. It is a potent UV protectant but also is quickly absorbed through the skin. While it has been approved by the FDA, there is a renewed interest in the safety of BP3, particularly in relation to breast cancer. The focus of this study was to examine the impact that BP3 has on triple negative breast cancer (TNBC) through alterations to cells in the immune microenvironment. In this study, we exposed female mice to one of two doses of BP3 before injecting them with a TNBC cell line. Several immune endpoints were examined both in the primary tissues and from <em>in vitro</em> studies of T cell behavior. Our studies revealed that in the lung tumor microenvironment, exposure to BP3 not only increased the number of metastases, but also the total area of tumor coverage. We also found that BP3 caused alterations in immune populations in a tissue-dependent manner, particularly in T cells. Taken together, our data suggest that while BP3 may not directly affect the proliferation of TNBC, growth and metastasis of TNBC-derived tumors can be altered by BP3 exposures via the alterations in the immune populations of the tumor microenvironment.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100080"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7e/59/nihms-1912439.PMC10434833.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nanovesicles based drug targeting to control tumor growth and metastasis 基于纳米囊泡的药物靶向控制肿瘤生长和转移
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2022.100083
Azim Ansari , Afzal Hussain , Raju Wadekar , Mohammad A. Altamimi , Abdul Malik , Md Ali Mujtaba , Mohammad Yousuf Ansari , Mohd Usman Mohd Siddique , Sameer N. Goyal
{"title":"Nanovesicles based drug targeting to control tumor growth and metastasis","authors":"Azim Ansari ,&nbsp;Afzal Hussain ,&nbsp;Raju Wadekar ,&nbsp;Mohammad A. Altamimi ,&nbsp;Abdul Malik ,&nbsp;Md Ali Mujtaba ,&nbsp;Mohammad Yousuf Ansari ,&nbsp;Mohd Usman Mohd Siddique ,&nbsp;Sameer N. Goyal","doi":"10.1016/j.adcanc.2022.100083","DOIUrl":"10.1016/j.adcanc.2022.100083","url":null,"abstract":"<div><p>Cancer is still a global challenge for healthcare professional and scientists due to complicated pathological pathways, inefficient early diagnosis, and limited safe delivery system at economic treatment cost. Despite these, other factors (life style, environmental problem, socio-economic issues, patient related complications, expensive therapy, and genetic history of oncogene) played significant role to spread and complicate treatment. However, various novel carriers have been explored and reported for effective and efficient drug delivery using polymers and lipid. Among them, vesicular systems are considered as the most biocompatible and safe for delivery of hydrophilic and lipophilic drug candidates. Therefore, the present review addressed various forms of nanovesicular systems with their benefits, progressive development stages, and mechanistic insights for drug targeting (active and passive), specific cancer wise nanovesicles, exosomes, and commercial products with potential clinical applications. The review primarily highlighted the major findings of nanovesicles employed to control solid tumor when a chemotherapeutic drug was used in specific vesicles based nanocarriers. Notably, miscellaneous exosomes, blood cells-based drug delivery (neutrophils and leukocytes), pH-responsive nanovesicles improved drug therapy by targeting tumor tissues and high drug access in the site of action. Finally, co-administration of chemotherapeutic drugs (combination therapy) further revealed convincing therapeutic outcomes as compared to standalone.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44203858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Immunomodulatory effects of β-defensin 2 on tumor associated macrophages induced antitumor function in breast cancer β-防御素2对肿瘤相关巨噬细胞诱导的乳腺癌抗肿瘤功能的免疫调节作用
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2023.100102
Sonam Agarwal, Anita Chauhan, Pramod Kumar Gautam
{"title":"Immunomodulatory effects of β-defensin 2 on tumor associated macrophages induced antitumor function in breast cancer","authors":"Sonam Agarwal,&nbsp;Anita Chauhan,&nbsp;Pramod Kumar Gautam","doi":"10.1016/j.adcanc.2023.100102","DOIUrl":"10.1016/j.adcanc.2023.100102","url":null,"abstract":"<div><h3>Background</h3><p>Tumor-associated macrophages (TAMs) express cytokines and chemokines that can suppress antitumor immunity and promote tumor progression. The immunomodulatory and antitumor function of β-defensin 2 is still unclear, despite the evidence of infection response. We previously reported that β-defensin 2 modulates immunomodulatory and their antitumor function of macrophages in breast cancer. We investigate the association between β-defensin 2 and TAMs and determined the role in tumor-promoting attributes of TAMs reversal of phenotype in tumor regression.</p></div><div><h3>Methods</h3><p>Swiss albino mice and C127i breast cancer cell line was used in this study. C127i conditioned media was prepared and generated macrophage-derived TAMs to study antitumor function. Flow cytometry was performed for phenotype identification of macrophages and TAMs. MTT assay was performed to estimate cytotoxicity and dose optimization of β-defensin 2. Oxidative stress was analyzed by H<sub>2</sub>O<sub>2</sub> and NO estimation, and qPCR was performed for iNOS, cytokines and chemokines expression.</p></div><div><h3>Results</h3><p>PEC harvested macrophages were characterized by flow-cytometry using F4/80, CD11c antibodies with 98% pure population of macrophages and cultured in C127i conditioned media for 7 days. TAMs markers were estimated, and it was found that 98% expression of F4/80, CD-206, and CD-115 expression compared to macrophages. Purified 100 ng/ml of β-defensin 2 was used to stimulate the TAMs population was viable, which was confirmed by cell viability assay. ROS levels decreased in TAMs treated with β-defensin 2 compared to control group. Interleukins (ILs)-6, 10, and 3, tumor necrosis factor (TNF)-α, and transforming growth factor (TGF)-β and chemokine ligand (CXCL)-1, 5 and 15, chemokine ligand (CCL)-24 and 5 decreased drastically compared to control.</p></div><div><h3>Conclusion</h3><p>This is the first report of β-defensin 2 on TAMs to elucidate the immunomodulatory and anti-tumor function. It was found that the cytokines, chemokines, and reactive oxygen species (ROS) expression pliably changed which facilitates tumor regression. β-defensin 2 must be targets as adjuvant for future cancer immunotherapeutic agent.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100102"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46546307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High expression of mesothelin in plasma and tissue is associated with poor prognosis and promotes invasion and metastasis in gastric cancer 血浆和组织中间皮素的高表达与胃癌预后不良有关,并促进胃癌的侵袭和转移
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2023.100098
Suryendu Saha , Chitranjan Mukherjee , Dipjit Basak , Prasun Panja , Pronoy Kanti Mondal , Ranajoy Ghosh , Aniket Halder , Abhijit Chowdhury , Gopal Krishna Dhali , Bitan Kumar Chattopadhyay , Saurabh Ghosh , Somsubhra Nath , Shalini Datta
{"title":"High expression of mesothelin in plasma and tissue is associated with poor prognosis and promotes invasion and metastasis in gastric cancer","authors":"Suryendu Saha ,&nbsp;Chitranjan Mukherjee ,&nbsp;Dipjit Basak ,&nbsp;Prasun Panja ,&nbsp;Pronoy Kanti Mondal ,&nbsp;Ranajoy Ghosh ,&nbsp;Aniket Halder ,&nbsp;Abhijit Chowdhury ,&nbsp;Gopal Krishna Dhali ,&nbsp;Bitan Kumar Chattopadhyay ,&nbsp;Saurabh Ghosh ,&nbsp;Somsubhra Nath ,&nbsp;Shalini Datta","doi":"10.1016/j.adcanc.2023.100098","DOIUrl":"10.1016/j.adcanc.2023.100098","url":null,"abstract":"<div><p>Mesothelin (MSLN), a tumor-associated antigen, is upregulated in various malignancies, including gastric cancer (GC). In addition, MSLN is found in the blood-stream of affected individuals, where it is referred to as soluble MSLN-related protein (SMRP). This study aims to investigate the role of MSLN in GC and evaluate its potential as a plasma biomarker for diagnosis and prognosis. Toward that end, GC tissues were obtained, upon signed consent, from affected individuals undergoing surgery or endoscopy (n = 82). Quantitative RT-PCR and immunohistochemistry were performed to determine MSLN expression. Simultaneously, The Cancer Genome Atlas (TCGA) database was mined to evaluate global status of MSLN gene expression in gastric cancer. Next, in vitro cell-culture studies were conducted to evaluate MSLN-driven proliferation properties. Using ELISA, sera from 55 GC-affected individuals were tested for MSLN level. Additionally, plasma mesothelin levels were compared in 6 cases before and after surgery. Upregulated MSLN expression was found in GC tissues, compared to adjacent normal tissues (p &lt; 0.001). Cell culture studies with a MSLN-overexpressing stable GC line showed increased cell proliferation and invasion with ectopic MSLN. Additionally, gene-set-enrichment-analysis (GSEA) revealed an association of MSLN with the genes involved in the epithelial-mesenchymal transition and G2/M checkpoint. GC-affected cases showed higher serum MSLN levels, compared to healthy controls, with rapid decrease post-surgery. We found that MSLN upregulation correlates with poor clinical outcome and promotes growth advantage to GC cells in vitro. With further experimental evidences, we propose that MSLN could potentially be used as a plasma biomarker for diagnosis of GC.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100098"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47880110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
miR-551a and miR-551b-3p target GLIPR2 and promote tumor growth in high-risk head and neck cancer by modulating autophagy miR-551a和miR-551b-3p在高危头颈癌中靶向GLIPR2,通过调节自噬促进肿瘤生长
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2022.100085
Narasimha Kumar Karanam , Lianghao Ding , Dat T. Vo , Uma Giri , John S. Yordy , Michael D. Story
{"title":"miR-551a and miR-551b-3p target GLIPR2 and promote tumor growth in high-risk head and neck cancer by modulating autophagy","authors":"Narasimha Kumar Karanam ,&nbsp;Lianghao Ding ,&nbsp;Dat T. Vo ,&nbsp;Uma Giri ,&nbsp;John S. Yordy ,&nbsp;Michael D. Story","doi":"10.1016/j.adcanc.2022.100085","DOIUrl":"https://doi.org/10.1016/j.adcanc.2022.100085","url":null,"abstract":"<div><p>The potential role for microRNA (miRNA) in the metastatic process that occurs in head and neck squamous cell carcinoma (HNSCC) was examined. miRNA was extracted from surgically excised tumor samples from 41 HNSCC cancer patients diagnosed with distant metastasis (DM) and from 53 patients who displayed no evidence of disease (NED) for a minimum of two years a minimum of two years after treatment with post-operative radiotherapy (PORT). A comparative two-way ANOVA of miRNA expression between DM and NED specimens identified 28 differentially expressed miRNAs with a false discovery rate (FDR) &lt; 0.2 and fold change &gt; 1.5. Two miRNA, miR-551a and miR-551b-3p, which share the same seed sequence, were associated with the DM group and with poor survival. Cell proliferation, migration, and invasion assays using the HN5 and UMSCC-17B HNSCC cell lines were performed after transfecting mimics or inhibitors of these miRNA uncovered an oncogenic role for miR-551a and miR-551b-3p. Furthermore, it was determined that miR-551a and miR-551b-3p directly target <em>GLIPR2</em> mRNA, a negative regulator of autophagy. Overexpression of GLIPR2 reduced proliferation, migration and invasion of HNSCC cells. In addition, overexpression of miR-551a and miR-551b-3p increased radioresistance while GLIPR2 overexpression increased the radiosensitivity of HNSCC cell lines. These results propose that the miR-551a, miR-551b-3p and GLIPR2 axis plays an important role in tumor growth, invasion and metastasis, at least in part by modulating autophagy and that the proliferative and pro-survival roles of miR-551a and miR-551b-3p may represent potential therapeutic targets by inhibiting autophagy through the regulation of GLIPR2 expression in HNSCC.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100085"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49864017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent advancement in breast cancer treatment using CAR T cell therapy:- A review 应用CAR T细胞疗法治疗癌症的最新进展-综述
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2023.100090
Anuvab Dey, Subhrojyoti Ghosh, Shreya Jha, Subhranil Hazra, Nainika Srivastava, Urmimala Chakraborty, Anupriya Guha Roy
{"title":"Recent advancement in breast cancer treatment using CAR T cell therapy:- A review","authors":"Anuvab Dey,&nbsp;Subhrojyoti Ghosh,&nbsp;Shreya Jha,&nbsp;Subhranil Hazra,&nbsp;Nainika Srivastava,&nbsp;Urmimala Chakraborty,&nbsp;Anupriya Guha Roy","doi":"10.1016/j.adcanc.2023.100090","DOIUrl":"10.1016/j.adcanc.2023.100090","url":null,"abstract":"<div><p>Breast Cancer arises to be the most diagnosed cancer type in recent decades. It ranks to be the most vulnerable among women in terms of incidence and mortality. In 2020, 2.3 million women were diagnosed with breast cancer, and 6.85 lacs of death were reported globally. Here, we will focus mainly on TNBC, the most complicated breast cancer subtype. Therefore, novel treatment modalities are urgently required. Treatments like chemotherapy and radiotherapy limit the efficacy of therapeutic outcomes. Thus, new specific ideas are coming up to find a way out. For triple-negative breast cancer (TNBC), which is currently the most complex and challenging breast cancer subtype to treat, chemotherapy is still the standard of care. There has been a lot of study into novel treatments for people with TNBC because of its poor prognosis and the high chance of clinical recurrence. Chimeric antigen receptor (CAR) T cell-based immunotherapy directs the patient's immune system to recognize and eradicate tumor cells that express tumor-associated antigens (TAAs). It opens up a new area of research. Chimeric Antigen Receptor (CAR-T) cell therapy is an immunotherapy type derived from adoptive T cell relocation. CAR-T cells are well equipped with specific antibodies to identify antigens in self-tumor cells, thus bringing out cytotoxic outcomes. CARs are the modified receptors with improved specificity and responsiveness to intensify the recognition of cancer cells. The therapeutic effects of CAR-T cell treatment, including breast cancer, have not lived up to expectations in solid tumors despite recent triumphs in treating hematologic malignancies. In this review, we will discuss some recent developments in the field of breast cancer-specific immunotherapy using CAR-T.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48666444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Modulation of gene expression associated with copy number variation identifies key regulatory programs in high-grade serous ovarian carcinoma 与拷贝数变异相关的基因表达调节确定了高级别浆液性卵巢癌的关键调控程序
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2023.100088
Martina Vescio , Lara Paracchini , Luca Beltrame , Maurizio D’Incalci , Sergio Marchini , Linda Pattini
{"title":"Modulation of gene expression associated with copy number variation identifies key regulatory programs in high-grade serous ovarian carcinoma","authors":"Martina Vescio ,&nbsp;Lara Paracchini ,&nbsp;Luca Beltrame ,&nbsp;Maurizio D’Incalci ,&nbsp;Sergio Marchini ,&nbsp;Linda Pattini","doi":"10.1016/j.adcanc.2023.100088","DOIUrl":"10.1016/j.adcanc.2023.100088","url":null,"abstract":"<div><p>High grade serous ovarian carcinoma (HGSOC) is a systemic malignancy characterized by metastatic lesions that spread within the peritoneal cavity. Despite initial sensibility to platinum-based chemotherapy, more than 80% of patients experience a relapse and acquire chemoresistance. From a genomic point of view, HGSOC shows a high level of inter- and intra-tumor heterogeneity. A better understanding of molecular mechanisms of this disease and the identification of driving genetic alterations could provide relevant indications for diagnostic and prognostic evaluation. Here, we accomplished a double-tier omic-analysis by integrating copy number variation data with matched gene expression profiling of multiple lesions in a cohort of 7 patients. We identified potential driver genes contained in amplified regions whose behavior seem to impact on gene expression program. They represent a distinctive signature that can segregate biopsies of different patients. Moreover, a further analysis highlighted <em>ZNF696</em>, <em>ASPSCR1</em> and <em>RHPN1</em> as key drivers, whose regulatory program is confirmed in TCGA cohort. In conclusion, exploration of gene expression program in HSGOC by integrating copy number and transcriptomic data from spatially separated samples obtained from seven patients led to the identification of genes whose amplification is significantly correlated to specific gene expression modules and are related to survival.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47403256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PFL-lectin regulates the expression of apoptosis-related proteins to antecedent apoptosis in A549 and HT29 cells PFL凝集素调节A549和HT29细胞凋亡相关蛋白的表达 单元格
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2023.100099
Arul Kumar Murugesan , Malairaj Sathuvan , Anand Javee
{"title":"PFL-lectin regulates the expression of apoptosis-related proteins to antecedent apoptosis in A549 and HT29 cells","authors":"Arul Kumar Murugesan ,&nbsp;Malairaj Sathuvan ,&nbsp;Anand Javee","doi":"10.1016/j.adcanc.2023.100099","DOIUrl":"10.1016/j.adcanc.2023.100099","url":null,"abstract":"<div><p>A potential treatment approach to treat this terminal illness is the mushroom-based lectin carrier system. It has been hypothesized that lectin-induced apoptotic nature causes necrosis, which leads to cell death, in cancer cells. The antibacterial and free radical scavenging capabilities of lectins were examined, according to the findings of our earlier lectin purification research. The goal of the current investigation is to determine whether <em>Pleurotus flabellatus</em> lectin (PFL-L) has any anti-cancer activity against colorectal cancer (HT29) and lung cancer (A549) cell lines. According to the findings of an in vitro cell line investigation, pre-treatment of the HT29 and A549 cell lines with PFL-L (10–100 μg/ml) significantly reduced the induction of apoptosis with an IC<sub>50</sub> range of PFL-L (67 &amp; 60 μg/ml). PFL-L protects cells against cancer cells, according to a confocal microscope viability examination of A549 and HT29 cells, and a comet test was used to track induced apoptosis. Our findings imply that PFL-L has promising anti-cancer activity and targets several apoptotic-related processes present in the A549 and HT29 cells. Additionally, when compared to the control, PFL-L increased DNA damage and the potential loss of cancer cells. A549 and HT29 cells also showed signs of the increased apoptosis-related proteins Bcl-Xl, Bcl-2, Procaspase-3, Procaspase-9, MMP-3, MMP-9, B6, N-Cadherin, and E-Cadherin. Western blot examination revealed decreased expression of apoptosis-related proteins. The results of this study demonstrate that PFL-L has anti-cancer properties against induced apoptosis in an in vitro model of A549 and HT29 cells.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"7 ","pages":"Article 100099"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47907291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment 转移性脑肿瘤免疫和细胞外基质微环境的空间分析
Advances in cancer biology - metastasis Pub Date : 2023-07-01 DOI: 10.1016/j.adcanc.2023.100096
Samuel S. Widodo , Marija Dinevska , Lucero Cuzcano , Michael Papanicolaou , Thomas R. Cox , Stanley S. Stylli , Theo Mantamadiotis
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