Advances in cancer biology - metastasis最新文献

{"title":"The effect of pDNA-Buforin II on the expression changes of lncRNAs PCA3, PCAT1, PRNCR1, GAS5 in prostate cancer","authors":"Fatemeh Dehkhodaei ,&nbsp;Abbas Doosti","doi":"10.1016/j.adcanc.2024.100130","DOIUrl":"10.1016/j.adcanc.2024.100130","url":null,"abstract":"<div><h3>Background</h3><div>This work aims to analyze the alterations in the levels of <em>PCA3</em>, <em>PCAT1</em>, <em>PRNCR1</em>, and <em>GAS5</em> long non-coding RNAs (lncRNAs) after the activation of pDNA-buforin II in PC3 cancer cells.</div></div><div><h3>Materials and methods</h3><div>The synthetic nucleic acid sequence of buforin II was included in the pcDNA3.1(+) Mammalian Expression Plasmid. The accuracy of cloning was assessed by using PCR and enzyme digestion techniques. The vectors were transfected into cells utilizing LipofectamineTM2000. The PC3 cancer cells were evaluated using flow cytometry and wound healing analysis. The expression levels of lncRNAs and apoptotic genes were assessed utilizing real-time PCR, with a significance threshold of P &lt; 0.05.</div></div><div><h3>Results</h3><div>The recombinant plasmid containing the pDNA-buforin II vector was successfully generated, and the gene sequence demonstrated complete uniformity (100 % similarity) with the buforin II gene. The transfection efficiency of PC3 cells was 79 %. The results are quantified utilizing the growth inhibition 50 % (GI50) parameter, representing the concentration of pDNA-buforin II required to halt 50 % of cell growth. The percentages of early apoptosis, late apoptosis, necrosis, and viable PC3 cells in the pDNA-buforin II group were 23.30 %, 12.70 %, 3.9 %, and 60.10 %, respectively. The RT-PCR study demonstrated that the presence of pDNA-buforin II in PC3 cells decreased the transcription of <em>PCA3</em>, <em>PCAT1</em>, and <em>PRNCR1</em> lncRNAs compared to the control group treated with PBS. Furthermore, it enhanced the transcription of <em>GAS5</em> lncRNA. The findings demonstrated a significant upregulation of transcription factors in programmed cell death after treatment with pDNA-buforin II (∗∗P &lt; 0.01).</div></div><div><h3>Conclusions</h3><div>According to the results of this study, it can be inferred that pDNA-buforin II can modify the transcription of genes in PC3 cancer cells, specifically about lncRNAs involved in cell apoptotic pathways. The pDNA-buforin II molecule has promising anticancer capabilities and can trigger apoptosis in cells.</div></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"12 ","pages":"Article 100130"},"PeriodicalIF":2.0,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZNF775 inhibits MCF-7 breast cancer cell migration by downregulating Wnt5a","authors":"Wei Gong ,&nbsp;Xin Zhu ,&nbsp;Wenwu Zhang ,&nbsp;Xiaoyu Song ,&nbsp;Junjie Hu ,&nbsp;Weihua Xu ,&nbsp;Zhichao Ma ,&nbsp;Bin Xiao ,&nbsp;Linhai Li ,&nbsp;Xinping Chen","doi":"10.1016/j.adcanc.2024.100129","DOIUrl":"10.1016/j.adcanc.2024.100129","url":null,"abstract":"<div><div>C2H2 zinc finger protein is widely involved in the occurrence and development of cancer. However, the function and mechanism of most C2H2 zinc finger proteins in breast caner (BC) remains unclear. Here, we reported the expression prognosis of C2H2 type zinc finger protein ZNF775 in BC patients and its possible biological mechanism. First, multiple public databases showed that ZNF775 was significantly overexpressed in BC tissues. Interestingly, high expression of ZNF775 was significantly associated with a better prognosis. Immunohistochemistry were used for verification, and the expression of ZNF775 was consistent with the databases. Considering the large heterogeneity of different breast cancer cells, we temporarily selected MCF-7 cell line for verification. In vitro overexpression experiments showed that overexpression of ZNF775 significantly inhibited the proliferation and migration of MCF-7 BC cell. We further combined RNA-sequencing (RNA-seq) and CUT &amp; Tag, and found that overexpression of ZNF775 can down-regulate the expression of most genes in the Wnt signaling pathway. The cBioportal database showed that ZNF775 was negatively correlated with the expression of Wnt5a, suggesting that its downstream target was likely Wnt5a. Finally, we discovered that Wnt5a could partially reverse the inhibitory effect of ZNF775 on MCF-7 BC cell migration through transwell migration experiments. In conclusion, our findings will provide new ideas for the diagnosis, treatment and prognosis assessment of BC in the future.</div></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"12 ","pages":"Article 100129"},"PeriodicalIF":2.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Niosomes containing enciprazine hydrochloride have been shown to efficiently inhibit the proliferation and induce apoptosis in colorectal cancer cells","authors":"Hosain Nasirian ,&nbsp;Saeedeh TarvijEslami ,&nbsp;Hedieh Ghourchian ,&nbsp;Marjaneh Ebrahimi ,&nbsp;Tohid Piri-Gharaghie ,&nbsp;Ghazal Ghajari","doi":"10.1016/j.adcanc.2024.100128","DOIUrl":"10.1016/j.adcanc.2024.100128","url":null,"abstract":"<div><p>Colorectal cancer (CRC), currently the second most widespread cancer globally, exhibits a higher incidence in young individuals. Advancements have been made in developing anti-colorectal cancer drugs, including cytotoxic chemicals, in the past few decades. There is a need for new and innovative medications to overcome the current challenges in cancer treatment. Recent research examined the efficacy of innovative formulations in the prevention of colorectal cancer. In this study, we evaluated the efficacy of a niosome formulation loaded with Enciprazine hydrochloride (Nio-USAN). We assessed the anti-colorectal cancer characteristics of Nio-USAN by employing several techniques including CCK-8, invasion test, MTT test, flow cytometry, and cell cycle assessment. Quantitative real-time PCR was utilized to assess the transcription of genes linked to apoptosis. The F1-Nio-USAN and F2-Nio-USAN have average sizes of 200 and 500 nm, respectively. The entrapment effectiveness (EE%) of the F1-Nio-USAN and F2-Nio-USAN was measured to be 85.32 ± 0.27 % and 87.12 ± 0.35 %, respectively. The F1-Nio-USAN group exhibited the following percentages of HT-29 cell states: 43 % early apoptosis, 21 % late apoptosis, 7 % necrotic, and 29 % viable. The levels of transcription for cas8, Bid, BAX, cas9, and cas3 were significantly elevated in the treatment groups as compared to the PBS control group (P &lt; 0.001). In addition, the treatment group exhibited significantly reduced levels of BCL2 gene transcription compared to the PBS control group (P &lt; 0.01). These results suggest that it may be possible to improve the efficacy of USAN formulations in combating cancer by utilizing noisome encapsulation.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"12 ","pages":"Article 100128"},"PeriodicalIF":2.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000157/pdfft?md5=06136d00907cdb5f01bc941b96159f7d&pid=1-s2.0-S2667394024000157-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating the interplay between BCL-2 family proteins, apoptosis, and autophagy in colorectal cancer","authors":"Amanda Shen-Yee Kong ,&nbsp;Sathiya Maran ,&nbsp;Hwei-San Loh","doi":"10.1016/j.adcanc.2024.100126","DOIUrl":"10.1016/j.adcanc.2024.100126","url":null,"abstract":"<div><p>Colorectal cancer (CRC) remains a significant global health challenge, with an alarming upward trend in Asia. Early detection is crucial for improving outcomes, but there is no consensus on the optimal screening approach. Despite advances in diagnosis and therapy, CRC mortality rates remain substantial. Apoptosis and autophagy, key processes in cancer cell death, exhibit complex molecular crosstalk, particularly involving BH-3-only proteins, which present potential therapeutic targets. Recent studies suggest that manipulating these pathways could enhance cancer treatment by exploiting their regulatory networks. The B-cell lymphoma 2 (BCL-2) family proteins, central to apoptosis regulation, are implicated in CRC initiation, progression, and therapy resistance. BH3-only proteins like BIM and PUMA are linked to caspase-independent cell death, suggesting alternative pathways for CRC treatment and highlighting the potential for targeted therapies. This review provides an overview of CRC management, including the current landscape and challenges of screening programs and delves into the interplay between apoptosis and autophagy in CRC cell death. It emphasizes the critical role of BCL-2 family proteins in CRC pathogenesis and calls for future research to focus on developing non-invasive, cost-effective diagnostic biomarkers, establishing prognostic biomarker panels, and defining predictive biomarkers for existing treatments. These advancements are essential for improving screening strategies, therapeutic interventions, and ultimately, patient outcomes and quality of life.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"11 ","pages":"Article 100126"},"PeriodicalIF":2.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000133/pdfft?md5=73acb2fe944f476babf22b8d43839a30&pid=1-s2.0-S2667394024000133-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141847129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PD-L1 and PD-1 in immune regulation and their implications in blood cancers","authors":"Parisa Shiri Aghbash ,&nbsp;Faezeh Mehdizadeh ,&nbsp;Ghazal Pourbeiragh ,&nbsp;Yalda Yazdani ,&nbsp;Hossein Bannazadeh Baghi ,&nbsp;Abolfazl Jafari Sales ,&nbsp;Mehrdad Pashazadeh ,&nbsp;Parisa Kangari","doi":"10.1016/j.adcanc.2024.100125","DOIUrl":"10.1016/j.adcanc.2024.100125","url":null,"abstract":"<div><p>Because of emerging opportunities for cancer immunotherapy, the capacity to suppress the immune system in order to cure and eradicate cancer is currently a topic of intense study. When the bone marrow microenvironment is exposed to immune suppression, leukemia cells result in the immune system's inability to eliminate malignant cells. To get a better understanding of the immunological possibilities associated with leukemia, clinical trials have explored immunotherapy techniques such as T cell activators, checkpoint inhibitors, antibody medicinal molecules, and cell treatments. One of the most important immune pathways is the programmed cell death 1 (PD1) protein. PD1 is expressed on the surface of T-cells and controls immune reactions. CD274, B7–H1, or PD-L1 are expressed by cells of the myeloid lineage, including macrophages, dendritic cells, effector CD8⁺ T cells, tumor cells, and tumor-associated suppressor cells. Expression of PD-L1 molecule in cancer has been associated to worse prognosis and resistance to anti-cancer therapies in several malignancies. In this review, we update on the expression of PD-1 molecule in malignant hematological tumor cells and describe these molecules which inhibit the immune response to cancer cells. We provide an overview of the current scientific advancements, the significance of immunotherapy strategies and highlighting the potential for further development in targeting this specific molecule. Additionally, ascertaining if PD-1/PD-L1 can be a reliable prognostic for blood cancer diagnosis.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"11 ","pages":"Article 100125"},"PeriodicalIF":2.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000121/pdfft?md5=00e0c675ef6dc26d06e116a1c581384a&pid=1-s2.0-S2667394024000121-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141851364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of mesenchymal stem cells and its exosomes in colorectal cancer: Paving way from preclinical towards clinical road","authors":"Zunaira Ali Baig ,&nbsp;Farzana Shafqat ,&nbsp;Iffat Mushtaq ,&nbsp;Ummara Aslam ,&nbsp;Asma Faryal ,&nbsp;Ayesha Maryam","doi":"10.1016/j.adcanc.2024.100123","DOIUrl":"https://doi.org/10.1016/j.adcanc.2024.100123","url":null,"abstract":"<div><p>Colorectal cancer is one of the most prevalent cancers worldwide. An increasing number of cases around the globe are raising concerns for life quality and survival. Various factors including genetic drivers have been extensively studied regarding the disease risk, progression, and metastasis. However, the signaling mechanisms haven't been studied extensively yet. Various therapeutic methods have been established in combating the disease, and mesenchymal stem cells have come up as a crucial cell-based therapeutic strategy. Mesenchymal stem cells have been regarded as potential targets in various cancer types due to their immune-modulatory functions. They can be isolated from many body tissues including bone marrow, peripheral blood, umbilical cord, and adipose tissue. Exosomes derived from mesenchymal stem cells have been reported to affect the expression of certain proteins associated with colorectal cancer. The current review highlights the potential of mesenchymal stem cells and their derived exosomes in treating cancer by causing cytotoxicity and apoptosis. Further, T-cell mediated modulation of exosomes helps reduce the cellular proliferation in cancer cells.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"11 ","pages":"Article 100123"},"PeriodicalIF":2.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000108/pdfft?md5=3a3361d8001405a8048789c9b7095c04&pid=1-s2.0-S2667394024000108-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141596396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The varied clonal trajectory of liver and lung metastases of colorectal cancer","authors":"Ofer N. Gofrit ,&nbsp;Ben Gofrit ,&nbsp;S. Nahum Goldberg ,&nbsp;Aron Popovtzer ,&nbsp;Jacob Sosna ,&nbsp;Ayala Hubert","doi":"10.1016/j.adcanc.2024.100122","DOIUrl":"https://doi.org/10.1016/j.adcanc.2024.100122","url":null,"abstract":"<div><h3>Background</h3><p>The liver and lungs are the most common sites of colorectal cancer (CRC) metastases. Their involvement can take five different clinical scenarios: lung metastases only, liver metastases only, lung metastases before liver metastases, liver metastases before lung metastases and simultaneous lung and liver metastases. Using clinical and morphological data we studied the clonal trajectory of these scenarios.</p></div><div><h3>Materials and methods</h3><p>A total of 465 (CRC) patients with 7952 liver and 6406 lung metastases were evaluated. Metastases clinical route was deciphered from metastases number, timing, and linear/parallel ratio (LPR)- a computerized parameter used for deducing clonal trajectories. LPR of +1 suggest pure linear dissemination and −1 pure parallel.</p></div><div><h3>Results</h3><p>Lung-only metastases: high percentage of metachronous disease with a long lead time and a low LPR suggest parallel dissemination. Liver-only metastases: Rare metachronous disease with a short lead time, and a high LPR suggest linear spread. Lung-before-liver metastases: rare solitary metastasis, a median gap of 21 months between the organs, high lung and low liver LPRs suggest linear progression to the lungs and parallel dissemination to the liver. Liver-before-lung metastases: low liver and high lung LPRs and a median gap of 16.5 months between the organs suggest parallel dissemination to the liver and linear spread from the liver to the lungs. Simultaneous liver and lung metastases: rare solitary metastasis and similar and high LPRs suggest simultaneous linear progression to both organs.</p></div><div><h3>Conclusions</h3><p>CRC metastases have different dissemination trajectories in different clinical scenarios. This information can potentially impact on clinical management.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"11 ","pages":"Article 100122"},"PeriodicalIF":2.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000091/pdfft?md5=6643c17257215aca6ddde3347b246b1c&pid=1-s2.0-S2667394024000091-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141596397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling cancer metastasis for more efficient therapeutic approaches","authors":"","doi":"10.1016/j.adcanc.2024.100120","DOIUrl":"10.1016/j.adcanc.2024.100120","url":null,"abstract":"","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"11 ","pages":"Article 100120"},"PeriodicalIF":2.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000078/pdfft?md5=f5378a0c97be77ce52e311880f8f2943&pid=1-s2.0-S2667394024000078-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microarray analysis of differentially expressed miRNA in triple negative breast cancer: A study of western India","authors":"Hemangini Vora ,&nbsp;Nikita Bhatt ,&nbsp;Dharvi Shah ,&nbsp;Prabhudas Patel ,&nbsp;Sonia Parikh ,&nbsp;Priti Trivedi ,&nbsp;Shashank Pandya","doi":"10.1016/j.adcanc.2024.100119","DOIUrl":"10.1016/j.adcanc.2024.100119","url":null,"abstract":"<div><h3>Background</h3><p>Triple-negative breast cancer (TNBC) is a genetically and morphologically heterogeneous group with aggressive biological behaviour and specific response to therapy. It accounts for 15–20% of all breast cancers and has two subtypes: basal like and non-basal like. MicroRNAs, which regulate gene expression, play a role in TNBC, potentially acting as oncogenes or tumor suppressors.</p></div><div><h3>Objective</h3><p>Identification of differentially expressed miRNA of potential clinical relevance in TNBC patients.</p></div><div><h3>Methods</h3><p>In this study, miRNA profiling by microarray was performed in tumor tissues of 86 patients with TNBC and 12 healthy individual. Further, the clinical relevance of differentially expressed miRNA was evaluated.</p></div><div><h3>Results</h3><p>In TNBC, 2410 differentially expressed miRNAs were identified and of them 98% were down-regulated, while only 2% were up-regulated. Up regulation of 55 miRNA was observed which target 16 genes. Top 5 genes identified were CDNK1A, p53, TGFB1, APC and HRAS. Of 7 ranking methods, 5 ranking method identified TGFB1 as most significant hub gene. Up regulated miRNA expression then compared between patients who undergone remission and patients who developed disease relapse and only miR-4532 was found upregulated in patients with disease relapse. Further, up regulation of miR-4532 showed a trend of reduced disease-free and overall survival. The down-regulated miRNAs target 238 genes involved in TNBC pathogenesis and progression. The top five hub genes were CDH1, PTEN, MYC, STAT3, and VEGFA. Of 7 ranking methods, 5 ranking method identified STAT3 as most significant hub gene. This study identified 32 novel miRNAs playing a tumor suppressive role and found down-regulated in TNBC. Among these two novel miRNAs, miR-1273g-3p and miR-4459 were found expressed in all TNBC patients. Patients with down-regulation of these miRNAs showed significantly reduced disease-free and overall survival. The ROC curve analysis indicated that miR-4532 and miR-4459 were successful in distinguishing TNBC patients from healthy controls.</p></div><div><h3>Conclusion</h3><p>Our data identified that up regulation of miR-4532 and down regulation of miR-4459 might have the potential to be used as both diagnostic and prognostic biomarker in TNBC.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"10 ","pages":"Article 100119"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000066/pdfft?md5=7edc26c17cfadb9b2aaa5bc22b8f50d5&pid=1-s2.0-S2667394024000066-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140275687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA expression profiling of leptin and adiponectin and its receptors in colorectal carcinoma – Biomarker development","authors":"Priyanka Parmesh ,&nbsp;Dinesh Udupi Shastri ,&nbsp;Mallikarjun Goni ,&nbsp;Anil Bapu Bargale ,&nbsp;Ajay Sathyanarayanrao Khandagale","doi":"10.1016/j.adcanc.2024.100118","DOIUrl":"https://doi.org/10.1016/j.adcanc.2024.100118","url":null,"abstract":"<div><h3>Background</h3><p>Metastatic colorectal carcinoma (CRC) is one of the leading causes of mortality of colorectal CRC. Very few prognostic markers and factors affecting progression are studied between metastatic CRC and early CRC. Adipokines are speculated to be associated with this area of interest. In the current study leptin, leptin receptor (Ob-R), adiponectin, adiponectin R1, and adiponectin R2 expression were measured at gene levels to determine a possible association between adipokines and early/metastatic cancer to ultimately identifying a definitive diagnostic marker.</p></div><div><h3>Materials and methods</h3><p>Tissue samples were obtained from 62 patients with radical specimens of CRC. Genes expression was determined by Quantitative Reverse-Transcription Polymerase Chain Reaction (qRT-PCR). The association between the clinicopathological parameters and gene expression levels were analyzed by statistical analysis.</p></div><div><h3>Results</h3><p>Overexpression of leptin mRNA is observed in small tumors (size&lt; 5 cms) <em>(P&lt;0.05).</em>b. Leptin receptor were overexpressed in advanced tumors (1.013 ± 0.152) than early tumors (0.453 ± 0.131). c.Adiponectin expression was higher in early tumors (1.535 ± 0.406) than in advanced tumors (0.48 ± 0.148). d. Distant organ metastasis shows under-expression of adipoR1. e.Overexpression of adipoR2 is seen in small (size&lt;5 cms<em>) (P&lt;0.05</em>) tumors.</p></div><div><h3>Conclusion</h3><p>LEPR is better indicator of advancement of tumor than Leptin. ADIPOR1 is a better indicator in advanced CRC than ADIPOR2 and ADIPOQ.</p></div>","PeriodicalId":72083,"journal":{"name":"Advances in cancer biology - metastasis","volume":"10 ","pages":"Article 100118"},"PeriodicalIF":0.0,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667394024000054/pdfft?md5=4db6465437748b306c5be46674351fde&pid=1-s2.0-S2667394024000054-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140123279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}