L Bisanti, F Bonetti, F Caramaschi, G Del Corno, C Favaretti, S E Giambelluca, E Marni, E Montesarchio, V Puccinelli, G Remotti, C Volpato, E Zambrelli, G M Fara
{"title":"Experiences from the accident of Seveso.","authors":"L Bisanti, F Bonetti, F Caramaschi, G Del Corno, C Favaretti, S E Giambelluca, E Marni, E Montesarchio, V Puccinelli, G Remotti, C Volpato, E Zambrelli, G M Fara","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Provisional data on selected sanitary events which took place at Seveso after July 10 1976 are reported. 187 cases of chloracne, mostly in children, were detected, 50 just after the accident, the others within a year. Most polluted area (zone A) provided almost all \"early\" and most severe cases, but the territorial distribution of chloracne prevalence rates showed some inconsistencies with the soil TCDD pollution map; interpretations for such findings are discussed. Thirty-eight birth defects were detected in 1977 (none in zones A and B), more than in previous years, but still less than expected in a well controlled \"normal\" population: no clustering around a given type was observed. Spontaneous abortions, evaluated both as abortion rates and as pregnancy loss rates, showed scattered and statistically non-significant variations, inconsistent with the pollution map. No differences in birth and death rates compared to surrounding areas were observed. Data on ad hoc cytogenetic, neurological and immunological surveys are commented. Limitations of the presently available data are discussed and further research lines are anticipated.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 1-2","pages":"139-57"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17509508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Distribution and fine structure of the interstitial cells of Cajal.","authors":"J Vajda, E Fehér","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Distribution and fine structure of the interstitial cells of Cajal were studied in the cat's small intestine. They were only found in the circular muscle layer. They had a slender structure with numerous mitochondria, rough endoplasmic reticulum and granular or secretory vesicles 100 to 300 nm in diameter. The closest distance between the membrane of smooth muscle cells and interstitial cells was 40 to 60 nm. Some nerve bundles were found in close relation to their processes, but neither pre- nor postsynaptic differentiation could be observed. After nialamide treatment the quantity of secretory vesicles increased and therefore it is suggested that these cells have a secretory function producing some kind of peptide and so they may act directly on the smooth muscle cells.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 3","pages":"251-8"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18456917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro methods for the study of the effects of teratogens on preimplantation embryos.","authors":"H Spielmann, H G Eibs, U Jacob-Müller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In vitro culture methods are described for mouse embryos during the preimplantation period (Whitten's medium) and for mouse and rat blastocysts during the time of implantation (NCTC-109 medium). Examples are given for the influence of the genetic background (strain differences) on in vitro development and for the detection of a DNA repair mechanism in cultured preimplantation embryos. S-shaped dose effect curves were obtained for the influence of UV-irradiation and cyclophosphamide (CPA) and its metabolites on development of pre implantation mouse embryos treated during in vitro culture. Similar curves were obtained for mouse blastocysts cultured at implantation after treatment of the mother with either CPA or the steroid sex hormones cyproterone acetate (CA) or medroxyprogesterone acetate (MPA). The embryo transplantation technique is described as a teratological method to test the viability of preimplantation mouse embryos which have incorporated the stable isotope 13C during in vitro culture. The importance of the in vitro culture techniques and of the transplantation method in experimental teratology during the earliest period of pregnancy is discussed.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 1-2","pages":"105-15"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18454081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of gonadotropins and thyrotropin on the testes and ovaries of the newly hatched chicken.","authors":"M A Shahin, G Csaba, O Dobozy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In newly hatched male and female chickens thyrotropin (TSH) and gonadotropins (FSH, LH) were found to increase the number of spermatogonia or Sertoli cells in the seminiferous cord, and the number of granulosa cells in the ovarian follicle. The effect of gonadotropins was more pronounced than that of TSH. Regarding the overlapping effect of thyrotropin and gonadotropins, in both male and female neonatal gonads the hormones similar in structure seem to be able to substitute each other. They do not however, change each other's effect on the parenchymatous tissue (number of spermatogonia and Sertoli cells in the male, and number of follicles in the female) if they are administered one after the other.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 4","pages":"317-35"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17833543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An air-drying method for preparing metaphase from spermatogonial cells of Chinese hamster (cricetulus griseus).","authors":"S Péter","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 3","pages":"309-11"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18052806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rental projections in the adult Xenopus laevis: a study with cobalt filling technique.","authors":"P Tóth, G Lázár, T Görcs","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cobaltic-lysine complex was used to show retinal projections in adult Xenopus laevis. Retinal fibres partially decussate in the chiasma, and terminate on both sides in the nucleus ovoidalis, lateral geniculate complex, pretectal area, optic tectum, and in the basal optic nucleus. Cobalt filled fibres were found in the preoptic area, dorsal hypothalamic area, and in the periventricular region close to the wall of the third ventricle.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 4","pages":"365-74"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18057124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Promoting effect of feeder cells in maintenance of adult rat hepatocytes.","authors":"A Tompa, R Langenbach","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A procedure is described for maintaining primary cultures of adult rat hepatocytes for prolonged periods of time on layer of irradiated mouse fibroblast cell line (C3H/1OT1/2) and on a secondary lung fibroblasts obtained from Sprague Dawley rats. Morphologically and ultrastructurally the cocultivated hepatocytes retained many characteristics of hepatocytes in vivo. Within 24 hours after seeding, the individual cells were attached on the feeder cell layer and the in vivo polarity of the liver cells reappeared. Electron microscope studies demonstrated the appearance of newly developed bile ducts and junctions between hepatocytes as well as between hepatocytes and feeder cells. Histochemically, these cells were positive for glucose-6-phosphatase and for glycogen. After 14 days in culture the hepatocytes could be reseeded onto fresh C3H1OT1/2 cells. In contrast, hepatocytes maintained on plastic substrate lost their glycogen content and the epithelial character of the liver cells after 5 days in culture, and by day 10 this culture became predominantly fibroblastic. It is suggested that hepatocytes maintained on an irradiated fibroblast feeder layer provide a valuable approach for studying the morphogenesis, cytotoxicity, or the metabolism of different chemicals in vitro.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 4","pages":"393-405"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17321073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Histological analysis of the overlapping effect of hypophyseal hormones on the cockerel's testes.","authors":"O Dobozy, G Csaba, M A Shahin, G Lazáry","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Histological examination of cockerel testes revealed the overlapping effects of FSH and TSH. Within the dose-range studied (20-160 microgram FSH and 27.5-220 microgram TSH), both hormones increased the cell number in the seminiferous cords including the number of spermatogonia, primary spermatocytes and pre-Sertoli cells. They also enhanced the mitotic activity of spermatogonia and accelerated spermatogenesis. Peak effects were observed after treatment with 160 microgram FSH and 55 microgram TSH dose. Liquefaction of cords due to hormone treatment was indicative of an acceleration of testicular ontogeny. Cross-effects of the two hormones were explained by receptor immaturity i.e. in the early stage of ontogenesis the receptors can bind both hormones due to the similarities in their structure. The maximum effects of the hormones were different, that of FSH being more marked.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 1-2","pages":"11-20"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17830784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Possible role of myofibroblasts in the pathogenesis of Dupuytren's contracture.","authors":"A Salamon, J Hámori","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the contracting palmar aponeurosis of patients suffering from Dupuytren's disease myofibroblasts are revealed by electron microscopical examination. These cells display abnormal foldings of the nucleus, microfilaments resembling the myofilaments in smooth muscle cells, rough endoplasmic reticulum occupying circumscribed portions of the cytoplasm, free ribosomes, well-developed Golgi apparatus, numerous mitochondria, occasionally lipid droplets, cilia-like formations, hemidesmosomes and maculae adherens (desmosomes) between the cells and formation of basement membrane. According to literary data, the myofibroblasts contain actin and have contractile properties. These myofibroblasts are supposed to contribute to the development of Dupuytren's contracture. The process is finally stabilized by the disappearance of myofibroblasts and by the simultaneous development of compact collagenous fibre bundles secernated by classical fibroblasts.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 1-2","pages":"71-82"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18206668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diurnal rhythm of hypophyseal cells in mice. Part III. Pars nervosa.","authors":"H Lach, S Krawczyk, K Dziubek, W Szaroma","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Karyometric analysis revealed a distinct diurnal rhythmicity of the nuclear volume of pars nervosa pituicytes in mice maintained on a 12 h light:dark schedule. Maximum nuclear volumes of pituicytes in both males and females were observed at 1800 o'clock while the minimum ones at 1200 o'clock. In addition, a distinct rhythm was observed in the amount of lipid vacuoles in the pituicytes of both sexes; they showed two peaks, a larger one at 1800 and a smaller one at 600 o'clock, as well as two minima at 1200 and 2400 o'clock.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"28 3","pages":"243-50"},"PeriodicalIF":0.0,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18206671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}