{"title":"An argyrophil III method for the demonstration of micro- and oligodendroglia.","authors":"F Gallyas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Micro- and oligodendroglia, plasma and nucleoli of nerve cells, capillary wall and nuclei of astrocytes become visible when sections of formol fixed human brain are immersed, without any previous treatment, into a physical developer of pH 10.5. The staining is inhibited by the catalytic activity of the tissue elements involved. By means of pretreatments with 1% performic acid and 30% sodium rhodanide dissolved in 0.4% sodium hydroxide, the catalytic activity in the unwanted tissue elements is suppressed, and this results in an elective demonstration of micro- and oligodendroglia. Reducing groups of the tissue or any kind of performed nuclei play no role in this silver staining.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 2-3","pages":"177-83"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17237357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multilamellar bodies in rat cerebellar Purkinje cells.","authors":"L Szegedy, I Balogh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Multilamellar bodies appearing in the rat's cerebellar Purkinje cells were studied ultrastructurally in methionine sulphoximine convulsions, hypovolaemic shock and carbon monoxide poisoning. It is concluded that multilamellar bodies are pathological formations due to toxic hypoxic factors.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"75-86"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18316852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Keratotic lesions of the oral epithelium.","authors":"I Rodriguez, M Martinez, J Miranda, J Bánóczy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Glycogen in the epithelium is interpreted as a sign of cell activity; its accumulation seems to take place gradually during cell migration. The oxido-reductase and hydrolytic enzyme-groups represent a sensitive indicator of changes in cell metabolism. The occurrence and intensity of glycogen, lactic and succinic dehydrogenases, NAD-cytochrome-c-reductase, alkaline and acid phosphatases were studied in 20 biopsy materials obtained from patients with keratotic buccal lesions. The results were compared with 20 biopsies from healthy oral mucosa. Variations were found in the localization and amount of glycogen, but no significant difference could be observed in the intensity of the oxido-reductase enzymes and alkaline phosphatase reactions. Acid phosphatase showed increased reactivity in most superficial layers of the keratinized epithelium.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"51-7"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18316927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Aseptic bone necrosis.","authors":"M Bély","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The post-traumatic and vascular necrosis of broken bones were studied in rabbits. AFter experimental traumatization and vascular lesion a similar type of necrosis was encountered. There were, however, differences in the distribution of the necrotic areas and in the position of the fracture-line. In the case of post-traumatic necrosis the fracture line was situated at the border of the intact and the necrosed tissue, whereas in vascular necrosis of secondarily broken bones it was found in the necrobiotic area. Surveying morphometrically the distribution of necrotic areas it was observed that in traumatic necrosis large fields occur on either side of the fracture-line which retained in 95% the nuclear staining of their osteocytes while in vascular necrosis such areas were not seen. On this basis traumatic and vascular necrosis can be differentiated provided the sample is taken from the surroundings of the fracture-line.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"59-74"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18316928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The role of megakaryocytes and tissue mast cells in the respiratory distress syndrome of adults.","authors":"L Kádas, K Széll","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The pathological role of megakaryocytes has been studied during adult respiratory distress syndrome (ARD) in the formation of intraalveolar oedema, microthrombi and hyaline membranes. The giant cells of the bone marrow are also thought to be involved in the development of peribronchial, perivascular and interalveolar oedemas. Heparin produced by tissue mast cells appears to counteract the clotting disturbances and to promote fibrinolysis in the acute phase of ARD. Vasoactive substances liberated from mast cells enhance oedema formation. Heparin has an influence on the lipid metabolism of type II pneumocytes. During regeneration the stimulating effect of heparin on fibrosis becomes predominant in the connective tissue ground substance. No fundamental morphological difference was found between ARD and the infantile respiratory distress syndrome.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 4","pages":"395-404"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18352178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of cyclophosphamide on the rat thymus.","authors":"N K Loc, I Oláh, L Takács, Z Kittner, Törö","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A single large dose (200 mg/kg) of cyclophosphamide (Cy) caused thymic involution. Thymic cortex was almost depleted of lymphocytes while medullary thymocytes were not affected. This resulted in thymic inversion on day 4. During the period of involution the cortex was invaded by positive acid phosphatase granules containing macrophages. Later the cytoplasms of these macrophages were loaded with lipid droplets and residual materials. Extensive vacuolization of the cytoplasms of epithelial and special cells were revealed. Regeneration began on day 4. Repopulating cells appeared primarily in subcapsular region. Regeneration was complete by day 11 as examined by morphological technics. While FCS dependent rosette formation capacity remained below the control level even on day 16.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 4","pages":"319-33"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18352432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Permanent amplifying effect on the cockerel gonad of hypophysis hormones (FSH, TSH) administered at hatching.","authors":"O Dobozy, M A Shahin, G Csaba","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of TSH and FSH was studied by quantitative histological analysis of the gonads in five-week-old cockerels pre-treated with these hormones at hatching. It was concluded that (1) hypophyseal hormones of similar molecular structure are not fully specific in immature animals. At five weeks both FSH and TSH enhance spermatogonium division and spermatogenesis, and increase the number of Sertoli cells. (2) While a single post-hatching dose of hormone has no effect on germinal cells as studied in the fifth week it increases the number of Sertoli cells (3) post hatching FSH-treatment facilitates the effect of hormone treatment at five weeks, amplifying the effect of FSH slightly more than that of TSH. Post hatching TSH-treatment also amplifies the effect of FSH administered at five weeks but does not influence the effect of five-week TSH-treatment on germinal cells. (4) The present results have corroborated earlier findings based on the measurement of gonadal weight and tubule diameter.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"19-25"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17848778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Changes of glucosaminoglycans in the goblet cells of bronchial epithelium during chronic bronchitis.","authors":"J Appel","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 1","pages":"91-102"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18316854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructure of human colorectal tumour xenografts maintained serially in immunosuppressed mice.","authors":"J Takács, L Kopper, K Lapis, C Hegedüs","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Five colorectal human tumour xenograft lines maintained by serial transplantation in immunosuppressed mice were studied by light and electron microscopy. Three had adenomatous while two had a mixed (adenomatous and mucinous) structure in the surgical specimens. After xenotransplantation in the mixed tumours the mucinous areas became dominant. Beside this there were no striking differences in the fine structure of the primarily implanted tumour compared with serially transplanted ones or between the different passages. The cells in adenomatous tumours formed acini with well-developed junctional complexes while the mucinous areas of mixed tumours were composed of single or loosely attached cells. In the former ones the cells had irregular microvilli on the apical surface and were separated from the stroma by a continuous basal lamina missing. The partially preserved functional activity was indicated by small vesicles in adenomatous, and mucin-droplets in mucinous tumours, showing intimate relationship with the rough endoplasmic reticulum and well-developed Golgi region. Some cells had features characteristic of both adenomatous and mucinous tumours. The ultrastructure of the studied tumours called attention to the possibility that malignant transformation may occur at different levels of cell differentiation.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 2-3","pages":"141-59"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18328504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Innervation of the cat ureter. An experimental study.","authors":"E Fehér, N B Salimova, D A Sakharov, J Vajda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Innervation of the ureter was studied by fluorescent microscopy using glyoxylic methods and by electron microscopy in the normal state and after extirpation of hypogastric ganglia. According to the morphology of the vesicle population, four groups of nerve processes could be distinguished: Type a with a dominant population of small agranular vesicles; Type b with a large number of small granular vesicles; Type c with 80 to 120 nm granulated vesicles; Type d with large 150 to 250 nm neurosecretory vesicles. After extirpation of the hypogastric ganglia, degenerated nerve terminals could be found in all the layers of the ureter, but a few fluorescent fibres could be observed in the muscle layers, too. It is assumed that most of the fluorescent fibres originate from the hypogastric ganglia and the others from the local neurons of from the spinal ganglia. These nerve processes act directly on the smooth muscle cells.</p>","PeriodicalId":72058,"journal":{"name":"Acta morphologica Academiae Scientiarum Hungaricae","volume":"29 4","pages":"353-9"},"PeriodicalIF":0.0,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18352434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}