Acta materia medica最新文献_第3页

Development and characterization of temperature-sensitive hydrogels embedded with sodium alginate-chitosan microspheres 海藻酸钠-壳聚糖微球包埋温度敏感水凝胶的制备与表征

Acta materia medica Pub Date : 2024-01-01 DOI: 10.15212/amm-2024-0017

Yan Yu, Xinrui Zhang, Xiaoyan Xu, Hang Zhou, Hongliang Wang, Yanfang Yang, Yuling Liu, Jun Ye

{"title":"Development and characterization of temperature-sensitive hydrogels embedded with sodium alginate-chitosan microspheres","authors":"Yan Yu, Xinrui Zhang, Xiaoyan Xu, Hang Zhou, Hongliang Wang, Yanfang Yang, Yuling Liu, Jun Ye","doi":"10.15212/amm-2024-0017","DOIUrl":"https://doi.org/10.15212/amm-2024-0017","url":null,"abstract":"Ulcerative colitis (UC) is a chronic intestinal disorder characterized by severe intestinal inflammation and superficial mucosal ulcers. Ramulus mori alkaloids (SZ-A) were approved by the National Medical Products Administration of China in 2020 for the treatment of type 2 diabetes mellitus. Later studies confirmed that SZ-A act on multiple inflammatory pathways to exert anti-inflammatory effects, suggesting that SZ-A has the potential to alleviate UC. A temperature-sensitive hydrogel embedded with sodium alginate (SA)-chitosan (CS) microspheres was constructed for colon-targeted delivery of SZ-A. The results showed that the microspheres prepared by exogenous emulsification had rounded and smooth surfaces with high drug-loading capacity. The optimum preparation conditions were as follows: 600 rpm stirring speed; a 1:4 ratio of water-to-oil phase; and a 5% Span80 emulsifier. The temperature-sensitive hydrogels embedded with SA-CS microspheres (TMH) had a moderate gelling temperature of 31.8 ± 0.74°C. The rheologic results showed that the internal structure of TMH was stable with viscoelasticity, and the in vitro release results indicated that the TMH exhibited slow-release performance. The findings suggest that TMH has suitable gelling temperature and viscoelasticity, and may be a promising targeted delivery vector for UC treatment.","PeriodicalId":72055,"journal":{"name":"Acta materia medica","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceopen.com/document_file/624fafcf-ede7-4be2-b157-2c88174307c1/ScienceOpen/amm20240017.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147881018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Polyphyllin II suppresses cell migration, invasion, and metastasis by inducing cytoskeletal rearrangement through the ROCK1/LIMK/CFL1 pathway in bladder cancer cells 在膀胱癌细胞中，聚phyllin II通过ROCK1/LIMK/CFL1通路诱导细胞骨架重排，从而抑制细胞迁移、侵袭和转移

Acta materia medica Pub Date : 2024-01-01 DOI: 10.15212/amm-2024-0043

Zhiyong Liu, Zhonghua Sun, Yuning Xie, Yaxin Shi, Guanghui Pan, Minmin Yu, Zilu Wang, Han Cao, Yang Liu, Qinbo Qiao, Changjing Chen, Xiangdong Xu, Qizhong Song, Zhenguo Wang

{"title":"Polyphyllin II suppresses cell migration, invasion, and metastasis by inducing cytoskeletal rearrangement through the ROCK1/LIMK/CFL1 pathway in bladder cancer cells","authors":"Zhiyong Liu, Zhonghua Sun, Yuning Xie, Yaxin Shi, Guanghui Pan, Minmin Yu, Zilu Wang, Han Cao, Yang Liu, Qinbo Qiao, Changjing Chen, Xiangdong Xu, Qizhong Song, Zhenguo Wang","doi":"10.15212/amm-2024-0043","DOIUrl":"https://doi.org/10.15212/amm-2024-0043","url":null,"abstract":"This study was aimed at determining the antimetastatic effect of polyphyllin II (PPII) in bladder cancer (BC) and the underlying mechanisms in vitro and in vivo . Wound healing, Transwell assays, and phalloidin staining were performed to determine the effects of PPII on BC cell migration, invasion, and cytoskeletal formation. Gene transcription and expression changes were detected via RNA sequencing and western blotting. The subchronic toxicity and antimetastatic effects of PPII were evaluated in Nu/Nu nude mice. PPII inhibited the migration and invasion of BC cells. Bioinformatics analysis indicated that cytoskeletal regulation was a potentially regulated process. PPII restrained cytoskeletal formation, as confirmed by phalloidin staining. Mechanistically, PPII was found to decrease p-LIMK1/2 and p-CFL1 expression through ROCK1, and to inhibit increased p-CFL1 levels and invasion and migration abilities of BC cells induced by constitutively active RHOA. Subchronic toxicity evaluation revealed that 3.0 mg/kg PPII had limited effects on tissue morphology, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase. Furthermore, PPII treatment effectively inhibited the formation of pulmonary metastatic nodules, as well as the expression of ROCK1, p-LIMK1, and p-CFL1 in the lungs. Thus, PPII inhibits BC cell invasion, migration, and metastasis through the RHOA-ROCK1-LIMK1/2-CFL1 axis, and is a potential candidate for antimetastatic drug development.","PeriodicalId":72055,"journal":{"name":"Acta materia medica","volume":"3 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147895114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Nose-to-brain drug delivery by HS15 micelles for brain targeting of insoluble drug HS15胶束经鼻至脑给药用于脑靶向不溶性药物

Acta materia medica Pub Date : 2024-01-01 DOI: 10.15212/amm-2023-0051

Wei Zhang, Long Wan, Meiqi Han, Guo Wen, Zhiqi Wang, Xinyue Zhang, Xuyang Liu, Jiahao Wang, Yuling Mao

{"title":"Nose-to-brain drug delivery by HS15 micelles for brain targeting of insoluble drug","authors":"Wei Zhang, Long Wan, Meiqi Han, Guo Wen, Zhiqi Wang, Xinyue Zhang, Xuyang Liu, Jiahao Wang, Yuling Mao","doi":"10.15212/amm-2023-0051","DOIUrl":"https://doi.org/10.15212/amm-2023-0051","url":null,"abstract":"Agomelatine (AGO) is an antidepressant with limited clinical application due to its low bioavailability caused by poor water solubility, severe hepatic degradation, and poor intracerebral distribution. Nanotechnology-based strategies constitute a powerful tool by utilizing drug solubility and targeted distribution to improve drug bioavailability in the brain tissues by nose-to-brain drug delivery. In the current study AGO was encapsulated within micelles using the commercially available Solutol HS15. As a result, AGO was efficiently loaded on the basis of least micelle size (14.17 ± 0.72 nm) and encapsulation efficiency (96.96%). In vivo and ex vivo experiments showed that micelles provided higher and more sustained brain distribution. And the results of pharmacokinetic experiments showed that micelles entered the brain region rapidly after intranasal administration and reach its peak within 0.25 h. The behavior of the nanoparticles indicated that the micelles were observed across the olfactory epithelium by examination of nasal mucosal penetration. The current study showed that HS15 micelles facilitate penetration of drugs in the olfactory epithelium and provide a system with which to target brain tissues and overcome the problem of inefficient drug delivery of insoluble drugs for central nervous system diseases.","PeriodicalId":72055,"journal":{"name":"Acta materia medica","volume":"3 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.scienceopen.com/document_file/af9fff27-4666-4965-9307-eeaefd07c6e0/ScienceOpen/amm20230051.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147912686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

PROTACs Targeting Epigenetic Proteins. 针对表观遗传蛋白质的 PROTACs。

Acta materia medica Pub Date : 2023-10-26 Epub Date: 2023-12-06 DOI: 10.15212/amm-2023-0039

Chao Zhang, Yuna He, Xiuyun Sun, Wenyi Wei, Yanlong Liu, Yu Rao

{"title":"PROTACs Targeting Epigenetic Proteins.","authors":"Chao Zhang, Yuna He, Xiuyun Sun, Wenyi Wei, Yanlong Liu, Yu Rao","doi":"10.15212/amm-2023-0039","DOIUrl":"10.15212/amm-2023-0039","url":null,"abstract":"<p><p>Epigenetics, a field that investigates alterations in gene function that can be inherited without changes in DNA sequence, encompasses molecular pathways such as histone variants, posttranslational modifications of amino acids, and covalent modifications of DNA bases. These pathways modulate the transformation of genotypes into specific phenotypes. Epigenetics plays a substantial role in cell growth, development, and differentiation by dynamically regulating gene transcription and ensuring genomic stability. This regulation is carried out by three key players: writers, readers, and erasers. In recent years, epigenetic proteins have played a crucial role in epigenetic regulation and have gradually become important targets in drug research and development. Targeted therapy is an essential strategy; however, the effectiveness of targeted drugs is often limited by drug resistance, posing a significant dilemma in clinical practice. Targeted protein degradation technologies, including proteolysis-targeting chimeras (PROTACs), have great potential in overcoming drug resistance and targeting undruggable targets. These areas of research are gaining increasing attention to various epigenetic related disease. In this review, we have provided a summary of the recently developed degraders targeting epigenetic readers, writers, and erasers. Additionally, we have outlined new applications for epigenetic protein degraders. Finally, we have addressed several unresolved challenges within the PROTAC field and offered potential solutions from our perspective. As the field continues to advance, the integration of these innovative methodologies holds great promise for addressing the challenges associated with PROTAC development.</p>","PeriodicalId":72055,"journal":{"name":"Acta materia medica","volume":"2 4","pages":"409-429"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364368/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploitation of platelets for antitumor drug delivery and modulation of the tumor immune microenvironment 血小板用于抗肿瘤药物递送和调节肿瘤免疫微环境

Acta materia medica Pub Date : 2023-05-13 DOI: 10.15212/amm-2023-0005

Jie Guo, Meng Wang, Yong Zhu, F. Watari, Yonghong Xu, Xiao Chen

{"title":"Exploitation of platelets for antitumor drug delivery and modulation of the tumor immune microenvironment","authors":"Jie Guo, Meng Wang, Yong Zhu, F. Watari, Yonghong Xu, Xiao Chen","doi":"10.15212/amm-2023-0005","DOIUrl":"https://doi.org/10.15212/amm-2023-0005","url":null,"abstract":"Platelets are blood components traditionally believed to have fundamental roles in vascular hemostasis and thrombosis. In recent years, platelets have received new attention for their roles in tumorigenesis and progression. On the one hand, platelets are actively recruited by various tumors and comprise a crucial part of the tumor microenvironment (TME), thus inspiring the use of platelets for tumor-targeted drug delivery. To this end, various platelet-based devices have been proposed, such as natural platelets, engineered platelets, platelet membranes, and platelet-derived microparticles. On the other hand, platelets are involved in tumor immunosuppression mechanisms, by directing and/or assisting various tumor-associated immune cells. However, in the context of inflammation and autoimmune diseases, platelets can amplify immune responses by promoting immune cell mobilization and activation, thereby exacerbating tissue damage. Thus, interest is growing in the use of tumor-associated platelets as targets for therapeutic modulation of the TME and augmenting anti-tumor immune responses. In this review, we summarize current advances in exploiting platelets for both antitumor drug delivery and immune modulation of the TME.","PeriodicalId":72055,"journal":{"name":"Acta materia medica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41947171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Emerging role of HJURP as a therapeutic target in cancers HJURP作为癌症治疗靶点的新作用

Acta materia medica Pub Date : 2023-05-06 DOI: 10.15212/amm-2023-0008

Jiaqi Tan, Lertnimitphun Peeraphong, Chattarin Ruchawapol, Juan Zhang, Juan Zhao, Wenwei Fu, Li Zhang, Hongxi Xu

引用次数: 2

Inhalable ciprofloxacin/polymyxin B dry powders in respiratory infection therapy 可吸入环丙沙星/多粘菌素B干粉治疗呼吸道感染

Acta materia medica Pub Date : 2023-04-29 DOI: 10.15212/amm-2022-0050

Zhengqi Xu, Hriday Bera, Hengzhuang Wang, Junwei Wang, Dongmei Cun, Yu Feng, Mingshi Yang

{"title":"Inhalable ciprofloxacin/polymyxin B dry powders in respiratory infection therapy","authors":"Zhengqi Xu, Hriday Bera, Hengzhuang Wang, Junwei Wang, Dongmei Cun, Yu Feng, Mingshi Yang","doi":"10.15212/amm-2022-0050","DOIUrl":"https://doi.org/10.15212/amm-2022-0050","url":null,"abstract":"The current study focused on the formulation, physicochemical characterization, and antibacterial susceptibility testing of inhalable spray dried powders containing ciprofloxacin (CIP) and polymyxin B sulfate (PMB). CIP nanosuspensions with an average particle diameter of 435.9 ± 9.3 nm were initially obtained using the wet-milling protocol and subsequently co-spray dried with PMB solutions to yield inhalable dry powders. The Powder X-Ray Diffraction (P-XRD) results showed that the wet-milled CIP nanoparticles were in a 4.8 hydrate state, which were transformed to 3.7 hydrates and amorphous materials after co-spray drying. The PMB remained in an amorphous state in the dry powders. Differential Scanning Calorimetry (DSC) analyses revealed that the glass transition temperatures (Tgs) of the co–spray dried formulations were higher than the Tg of CIP, but lower than the Tg of PMB. Fourier Transform Infrared Spectrometer (FTIR) studies suggested the existence of π - π interactions between CIP and PMB in the co-spray dried powders. These powders also retained antimicrobial effects against Pseudomonas aeruginosa strain PAO1. In addition, the spray-dried powder formulations exhibited satisfactory solid-state stability and aerodynamic characteristics when stored under 3% relative humidity and 20 ± 5 °C for 4 months. Overall, the newly developed inhalable CIP/PMB dry powders are a promising therapeutic strategy for respiratory tract infections.","PeriodicalId":72055,"journal":{"name":"Acta materia medica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44299684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular networking as a natural products discovery strategy 分子网络作为一种天然产物发现策略

Acta materia medica Pub Date : 2023-04-25 DOI: 10.15212/amm-2023-0007

Mi Zhang, Kouharu Otsuki, Wei Li

引用次数: 1

Multi-component Chinese medicine formulas for drug discovery: State of the art and future perspectives 用于药物发现的多组份中药配方:现状和未来展望

Acta materia medica Pub Date : 2023-03-30 DOI: 10.15212/amm-2022-0049

Cheng Zhang, Guoming Chen, Guoyi Tang, Xiaoyu Xu, Z. Feng, Yuanjun Lu, Y. Chan, Junyu Wu, Yuanyuan Chen, Lin Xu, Qing-li Ren, Hongchao Yuan, Dong Yang, Zhe-Sheng Chen, Ning Wang, Yibin Feng

{"title":"Multi-component Chinese medicine formulas for drug discovery: State of the art and future perspectives","authors":"Cheng Zhang, Guoming Chen, Guoyi Tang, Xiaoyu Xu, Z. Feng, Yuanjun Lu, Y. Chan, Junyu Wu, Yuanyuan Chen, Lin Xu, Qing-li Ren, Hongchao Yuan, Dong Yang, Zhe-Sheng Chen, Ning Wang, Yibin Feng","doi":"10.15212/amm-2022-0049","DOIUrl":"https://doi.org/10.15212/amm-2022-0049","url":null,"abstract":"For hundreds of years, the drug discovery and development industry has aimed at identifying single components with a clear mechanism of action as desirable candidates for potential drugs. However, this conventional strategy of drug discovery and development has faced challenges including a low success rate and high development costs. Herein, we critically review state-of-the-art drug discovery and development based on multi-component Chinese medicine formulas. We review the policies and application status of new drugs based on multi-component Chinese medicines in the US, China, and the European Union. Moreover, we illustrate several excellent cases of ongoing applications. Biomedical technologies that may facilitate drug discovery and development based on multi-component Chinese medicine formulas are discussed, including network pharmacology, integrative omics, CRISPR gene editing, and chemometrics. Finally, we discuss potential problems and solutions in pre-clinical and clinical research in drug discovery and development based on multi-component Chinese medicine formulas. We hope that this review will promote discussion of the roles of multi-component Chinese medicine formulas in the discovery and development of new drugs for the treatment of human diseases.","PeriodicalId":72055,"journal":{"name":"Acta materia medica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43989988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Discovery of a pyridophenoselenazinium-based photosensitizer with high photodynamic efficacy against breast cancer cells 发现一种对乳腺癌症细胞具有高光动力效应的基于吡啶并苯并氮嗪的光敏剂

Acta materia medica Pub Date : 2023-03-23 DOI: 10.15212/amm-2023-0002

Guiling Li, Peixia Li, Qiaoyun Jiang, Qianqian Zhang, Jingru Qiu, Donghai Li, Gang Shan

引用次数: 0