Advanced Studies in Biology最新文献_第8页

Pharmacochemistry of Iranian flora Artemisia oliveriana and its antimalarial effects on Plasmodium berghei in vivo 伊朗植物艾草的体内药物化学及其对伯氏疟原虫的抗疟作用

Advanced Studies in Biology Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.61141

Touraj Miandoabi, K. H. Tappeh, H. Nahrevanian, Seyed Masoud Kazemi, H. Mohammadzadeh, Haleh Hanifian

{"title":"Pharmacochemistry of Iranian flora Artemisia oliveriana and its antimalarial effects on Plasmodium berghei in vivo","authors":"Touraj Miandoabi, K. H. Tappeh, H. Nahrevanian, Seyed Masoud Kazemi, H. Mohammadzadeh, Haleh Hanifian","doi":"10.12988/ASB.2017.61141","DOIUrl":"https://doi.org/10.12988/ASB.2017.61141","url":null,"abstract":"The aim of this study was to evaluate antimalarial effects of Iranian flora Artemisia oliveriana on Plasmodium berghei in vivo and photochemistry of its natural effective components. This is the first application of Iranian flora A. oliveriana on murine malaria in Iran. The aerial parts of A. oliveriana were collected at the flowering stage from Qum and Isfahan provinces, in central part of Iran in 2010. The aerial parts were air-dried at room temperature and then powdered and macerated in methanol and filtered and it was extracted and dried by Rotary Evaporator. The toxicity of herbal extract was assessed on four groups of naive NMRI mice and it‘s antimalarial efficacy was investigated on infected P. berghei animals. The significance of differences was determined by student‘s ttest using Graph Pad prism software. The results indicated no toxicity was observed even by high concentration of herbal extract by measuring body weight, survival rate and hepato/ splenomegaly. It is demonstrated that total extract A. oliveriana possesses therapeutic inactivity against P. berghei which indicates its antimalarial effects in vivo on NMRI mice. 44 Touraj Miandoabi et al.","PeriodicalId":7194,"journal":{"name":"Advanced Studies in Biology","volume":"57 72 1","pages":"43-51"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77689343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NSGA-II for biological graph compression NSGA-II用于生物图压缩

Advanced Studies in Biology Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.61143

A. Zakirov, J. A. Brown

引用次数: 6

How water quality in the Kabul River, Pakistan, can be determined with algal bio-indication 巴基斯坦喀布尔河的水质如何通过藻类生物指示来确定

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/ASB.2016.6830

S. Barinova, Izaz Khuram, Asadullah, N. Ahmad, Samin Jan, D. Shin

{"title":"How water quality in the Kabul River, Pakistan, can be determined with algal bio-indication","authors":"S. Barinova, Izaz Khuram, Asadullah, N. Ahmad, Samin Jan, D. Shin","doi":"10.12988/ASB.2016.6830","DOIUrl":"https://doi.org/10.12988/ASB.2016.6830","url":null,"abstract":"Altogether 209 species of algae and cyanobacteria has been revealed in 2014-2015 from four sites in the Kabul River that flow across the Peshawar Valley. Green algae, diatoms, and Charophyta filamentous algae were diverse and characterize regional feature in the river basin with high agricultural activity. Species richness and algal abundance were increase down the river. Index saprobity S varied between 1.55 and 1.59 (Class III of water quality) and reflects decreasing of water quality down the river as a result of pollutants impact, which brings mostly the Swat tributary. Bio-indication results show prevalence of benthic or plankto-benthic species, temperate temperature indicators, slow streaming middle oxygenated waters inhabitants, salinity-indifferents, eurysaprobes, mesotrophes, and autotrophic algae with tolerance to middle concentration of Nitrates that reflects middle polluted waters III-IV Class of Water 152 Sophia Barinova et al. Quality in the Kabul River. Our analysis shows that integral bio-indication in water quality assessment with using of algal communities can give relevant results of self-purification possibility, which can be employed in purpose of monitoring the regional water quality as economy and resultative method.","PeriodicalId":7194,"journal":{"name":"Advanced Studies in Biology","volume":"24 1","pages":"151-171"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81301571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Cloning, expression and purification of recombinant A2 protein from Leishmania infantum for diagnosis of visceral leishmaniasis in Iran 伊朗婴儿利什曼原虫重组A2蛋白的克隆、表达和纯化及其在内脏利什曼病诊断中的应用

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/ASB.2016.6413

Sanaz Naderi, H. Nahrevanian, V. Khalaj, M. Farahmand

{"title":"Cloning, expression and purification of recombinant A2 protein from Leishmania infantum for diagnosis of visceral leishmaniasis in Iran","authors":"Sanaz Naderi, H. Nahrevanian, V. Khalaj, M. Farahmand","doi":"10.12988/ASB.2016.6413","DOIUrl":"https://doi.org/10.12988/ASB.2016.6413","url":null,"abstract":"Visceral leishmaniasis (VL) is a fatal disease caused by Leishmania infantum in the Mediterranean basin and Iran. Different methods are used for diagnosis of VL. The aims of this study were expression and purification of recombinant A2 (rA2) protein of L.infantum and its application in the diagnosis of VL. The serological diagnosis of VL was applied using rA2 protein. In this study, A2 gene of L.infantum sequence was ordered for the synthesis, cloned in E.coli strain TOP10F' and proliferated in pET22-b vector. The expression and purification of rA2 proteins applied in host via BL21 and Ni-NTA respectively. The A2 gene sequences were synthesized and the construct transformed to pET22-b vector. A 102 Sanaz Naderi et al. 520bp fragment was identified in digested pEASY-A2 plasmid. The gene was successfully cloned in to pET22-b standard expression vector and transformed in E.coli BL21. Expression of rA2 was confirmed by SDS-PAGE and a 27KD protein was detected. The antigenicity of A2 protein was assessed using both pooled dog sera and C9 anti-A2 monoclonal Ab. This study recommends rA2ELISA as alternative assay to detect VL. More evaluation should be made to develop a cheap and reliable serologic test for detection of L.infantum among infected hosts.","PeriodicalId":7194,"journal":{"name":"Advanced Studies in Biology","volume":"2016 1","pages":"101-110"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87950960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic structural hypothesis of aging 老龄化的系统结构假说

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/ASB.2016.612

A. Kolomiytsev

{"title":"Systemic structural hypothesis of aging","authors":"A. Kolomiytsev","doi":"10.12988/ASB.2016.612","DOIUrl":"https://doi.org/10.12988/ASB.2016.612","url":null,"abstract":"Phenomenon of aging came to existence simultaneously with formation of cell associations and multicellular organisms and played an adaptive role at the early stages of evolution. This phenomenon may be the result of formation of mechanisms that control cellular growth in compliance with nutrient factors limit. Multicellular organisms function as complex systems where the cell division is regulated both by intracellular processes and general systemic organization. The developmental process is determined by a kinetic curve of population growth, which is typical of every cell association. The systemic structural hypothesis considers every multicellular organism as a system that consists of various cellular associations in symbiotic interaction. One of these associations limits the developmental kinetics of the whole organism. Life duration is determined by potential of limiting cell association stem cells. It is offered to introduce a special term – regeneration submodule a unit that consists of cells and tissues which have a general regeneration complex – a group of stem cells that renovate strictly these cells and tissues. Such regeneration submodules are formed within organs and systems; one of them is a dominating unit which determines the life duration.","PeriodicalId":7194,"journal":{"name":"Advanced Studies in Biology","volume":"4 1","pages":"17-25"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83395027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of Stachys lavandulifolia leaves aqueous extract on angiogenesis in chick embryos chorioalantoic membrane and choroids plexus 花楸叶水提物对鸡胚绒毛膜、尿囊膜和脉络丛血管生成的影响

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/ASB.2016.638

M. Tehranipour, S. Balanezhad

{"title":"The effects of Stachys lavandulifolia leaves aqueous extract on angiogenesis in chick embryos chorioalantoic membrane and choroids plexus","authors":"M. Tehranipour, S. Balanezhad","doi":"10.12988/ASB.2016.638","DOIUrl":"https://doi.org/10.12988/ASB.2016.638","url":null,"abstract":"Angiogenesis, formation of new vessels from the primary vessels is involved in physiological condition such as reproduction, wound healing and pathological like Diabetes and tumor. Tumor progression associates with persistent unregulated angiogenesis. This study aimed to investigate the effect of leaves aqueous extract of Stachys lavandulifolia on the angiogenesis in chorioallantoic membrane and choroids plexus of chick embryos. In this experimental study, 30 fertilized eggs of Hy-line race were randomly divided into 5 groups: control group, sham-exposed group and four experimental groups (n=6). In the second day of incubation in sterile condition, a window was opened on eggs. On 8th day, chorioallantoic membrane (CAM) was treated at sham-exposed group with normal saline and at experimental groups with Stachys lavandulifolia aqueous extract (25, 50, 100 mg/kg). On 12th day CAMs, the length and number of vessels were examined and 66 Maryam Tehranipour and Saeide Zafar Balanezhad morphological disorders were studied. Data were calculated and analyzed statistically using t-test and ANOVA. There is no significant difference in the mean number and length of vessels in the control group with the sham-exposed groups. The mean of number and length in all experimental groups shows a meaningful increase in compare with sham group (P<0/05).Morphological abnormalities were not observed in fetuses. According to the results of this research, low doses of Stachys lavandulifolia aqueous extract increase angiogenesis in chorioallantoic membrane and choroids plexus, but it has antiangiogenesis effect in high doses. No significant effect on chick embryo's morphological characteristics observed.","PeriodicalId":7194,"journal":{"name":"Advanced Studies in Biology","volume":"117 1","pages":"65-75"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86800301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety evaluation of Picrorhiza kurroa rhizome extract by bacterial reverse mutation test 用细菌反突变试验评价黑根茎提取物的安全性

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/asb.2016.6620

A. Balkrishna, H. K. Manikyam, V. Sharma, Niti Sharma

引用次数: 2

A wavelet analysis to classify the interspike activity of peptidergic neurons 小波分析对肽能神经元峰间活动的分类

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/ASB.2016.6829

R. Á. Pozos, Andrey M. Montoya, Gladys Elena Salcedo Echeverry

引用次数: 0

Comparative anatomical studies on petioles of Nepeta L. species (Lamiaceae) in NE Iran 标题伊朗东北部荆芥属植物叶柄的比较解剖学研究

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/ASB.2016.6728

S. M. M. Shahri, A. Jafari, H. Mahmoodzadeh

引用次数: 7

Design and construction of household composter and detection of extended spectrum β-lactamase producing Pseudomonas aeruginosa and Enterobacteriaceae in compost 家用堆肥机的设计与构建及堆肥中产β-内酰胺酶铜绿假单胞菌和肠杆菌科菌的检测

Advanced Studies in Biology Pub Date : 2016-01-01 DOI: 10.12988/ASB.2016.626

E. Yousefi, M. N. Moghaddam

{"title":"Design and construction of household composter and detection of extended spectrum β-lactamase producing Pseudomonas aeruginosa and Enterobacteriaceae in compost","authors":"E. Yousefi, M. N. Moghaddam","doi":"10.12988/ASB.2016.626","DOIUrl":"https://doi.org/10.12988/ASB.2016.626","url":null,"abstract":"Today, generation of waste is increasing and despite the advancement of science and technology, its management has changed into a complex problem. The aim of this study was to reduce solid waste from the source by designing and building home composter and also detect of extended spectrum β-lactamase (ESBL)producing Pseudomonas aeruginosa and Enterobacteriaceae isolates in composting processes by phenotypic and molecular methods. AUTOCAD, Photoshop and 3ds MAX were used to model and design the components of device. Composter was built using galvanized iron sheet with the dimensions 35 × 36 × 32 cm and its defects were resolved step by step. The effect of produced compost was determined on the growth of selected plants. Gram negative bacteria were isolated from the materials undergoing composting, the leachate, and mature compost in two repetitions. Composting process took long 38 days. 40 Ehsan Yousefi and Mahboobeh Nakhaei Moghaddam Produced compost significantly increased the growth of cranesbill and wheat in comparison with the growth hormone and chemical fertilizer (p<0.05). The frequency of bacteria isolated in the initial waste was larger than in the maturity stage. Antibiotic resistance was low among isolates and none of them were ESBLproducer. Colony PCR revealed that all isolates harbored the oprL and exoA genes. The results showed that the built composter had a good efficiency and the produced compost could be used as a suitable alternative to chemical fertilizers.","PeriodicalId":7194,"journal":{"name":"Advanced Studies in Biology","volume":"73 1","pages":"39-52"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72990147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0