Design and construction of household composter and detection of extended spectrum β-lactamase producing Pseudomonas aeruginosa and Enterobacteriaceae in compost

Today, generation of waste is increasing and despite the advancement of science and technology, its management has changed into a complex problem. The aim of this study was to reduce solid waste from the source by designing and building home composter and also detect of extended spectrum β-lactamase (ESBL)producing Pseudomonas aeruginosa and Enterobacteriaceae isolates in composting processes by phenotypic and molecular methods. AUTOCAD, Photoshop and 3ds MAX were used to model and design the components of device. Composter was built using galvanized iron sheet with the dimensions 35 × 36 × 32 cm and its defects were resolved step by step. The effect of produced compost was determined on the growth of selected plants. Gram negative bacteria were isolated from the materials undergoing composting, the leachate, and mature compost in two repetitions. Composting process took long 38 days. 40 Ehsan Yousefi and Mahboobeh Nakhaei Moghaddam Produced compost significantly increased the growth of cranesbill and wheat in comparison with the growth hormone and chemical fertilizer (p<0.05). The frequency of bacteria isolated in the initial waste was larger than in the maturity stage. Antibiotic resistance was low among isolates and none of them were ESBLproducer. Colony PCR revealed that all isolates harbored the oprL and exoA genes. The results showed that the built composter had a good efficiency and the produced compost could be used as a suitable alternative to chemical fertilizers.