Acta Scientiarum. Biological Sciences最新文献_第2页

In vitro antimicrobial activity of the crude extract of the endophytic bacterium Pseudomonas aeruginosa (SS93) isolated from Sapindus saponaria L. 从无患子中分离出的铜绿假单胞菌（SS93）内生菌粗提取物的体外抗菌活性

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.65524

Adriana Garcia, Caroline Menicoze dos Santos, A. D. Polli, J. C. Polonio, João Arthur dos Santos de Oliveira, Marcos Alessandro dos Santos Oliveira, Halison Correia Golias, João Alencar Pamphile

{"title":"In vitro antimicrobial activity of the crude extract of the endophytic bacterium Pseudomonas aeruginosa (SS93) isolated from Sapindus saponaria L.","authors":"Adriana Garcia, Caroline Menicoze dos Santos, A. D. Polli, J. C. Polonio, João Arthur dos Santos de Oliveira, Marcos Alessandro dos Santos Oliveira, Halison Correia Golias, João Alencar Pamphile","doi":"10.4025/actascibiolsci.v45i1.65524","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.65524","url":null,"abstract":"Endophytes colonize the interior of plant tissues without causing any damage to their hosts. The plant Sapindus saponaria L., popularly known as ‘sabão-de-soldado’, presents a diversified endophytic microbiota and also medicinal properties. Endophytic microorganisms may produce secondary metabolites with different biotechnological properties. The present study aimed to evaluate the in vitro antibacterial and antifungal capacity of the crude extract of secondary metabolites produced by the endophytic bacteria P. aeruginosa SS93 isolated from S. saponaria leaves. The metabolites extract was obtained using the organic solvent ethyl acetate, and the antimicrobial activities were tested against six pathogenic bacteria (Enterococcus faecalis [ATCC 29212], Pseudomonas aeruginosa [ATCC 27853], Shigella flexneri [ATCC 12022], Salmonella enterica [CCCD a016], Escherichia coli [ATCC 25922], and Staphylococcus aureus [ATCC 25923]), and pathogenic fungi (Fusarium oxysporum, Glomerella sp., Sphaceloma sp., Fusarium solani, Maniliophtora perniciosa, and Sclerotinia sclerotiorum), by agar diffusion method. In the antibacterial assay, the best results were obtained against E. faecalis and S. aureus, where the formation of inhibition halos was observed in all tested concentrations, especially at 500 and 700 µg mL-1. Positive inhibitory activity against phytopathogenic fungi was observed, with the highest inhibition recorded against F. oxysporum (61.1%), followed by Sphaceloma sp. (55.7%), M. perniciosa (35.6%), F. solani (34.4%), and Glomerella sp. (30.4%).","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"186 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenological growth stages of biquinho pepper according to the BBCH scale 根据 BBCH 分级法确定的生物喹诺酮辣椒的各生长阶段

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.63742

Paula Andriessa Krause, M. Dotto, Kelli Pirola, Elouize Xavier, Camila Moreno Giarola, A. R. Lopes

引用次数: 0

Optimization of treatments for the germination and establishment of botanical yam (Dioscorea rotundata) seeds 植物山药（薯蓣）种子发芽和成活的优化处理方法

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.65008

K. Nwagu, C. Udechukwu, M. Ukaegbu, Evelyn Nwakaego Enwere, Adaugo Gift Ibeh, Fredrick Ogeh Okpaga, Alex Ochai Okpe

{"title":"Optimization of treatments for the germination and establishment of botanical yam (Dioscorea rotundata) seeds","authors":"K. Nwagu, C. Udechukwu, M. Ukaegbu, Evelyn Nwakaego Enwere, Adaugo Gift Ibeh, Fredrick Ogeh Okpaga, Alex Ochai Okpe","doi":"10.4025/actascibiolsci.v45i1.65008","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.65008","url":null,"abstract":"Botanical yam (Dioscorea rotundata) seeds are currently used by yam breeders to generate and conserve new varieties of yam instead of seed yams (vegetative propagule). Yam seed treatment through scarification is one of the most effective methods of improving seed germination and seedling establishment. This study was carried out under greenhouse condition to determine the best seed treatment for the germination and establishment of botanical yam seeds. The set up involved a soil mixed with carbonized rice husk using five different seed treatments; dry heating (DHS) at 60 0C, mechanical scarification + gibberellic acid (MSS + GA), mechanical scarification (MSS), acid scarification with sulphuric acid (ASS) and soaking in hot water at 80 0C (SOS). The findings showed that the treatment, MSS + GA and MSS only, had significant (P<0.05) positive effect on the seed emergence and seedling establishment compared to the untreated control (COS) and other treatments. ASS caused seed damage resulting to no emergence. The findings showed that carbonized grain waste (rice husk) enhanced soil fertility and seed treatment (MSS + GA) was the best to improve yam seed germination and seedling establishment. These treatments can be efficiently applied to reduce the long gestation periods and increase yield in yam breeding programmes.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute toxicity study of the isomeric mixture of alpha and beta amyrin from Protium heptaphyllum (Aubl.) Marchand 来自马钱子七叶树（Protium heptaphyllum (Aubl.) Marchand）的 alpha 和 beta amyrin 异构体混合物的急性毒性研究

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.66144

G. Aragão, A. O. Nogueira, F. A. F. Xavier Júnior, Janaina Serra Azul Monteiro Evangelista, Paulo Nogueira Bandeira, Camila Fernandes, Maria Elisabete Amaral de Moraes, A. Assreuy

{"title":"Acute toxicity study of the isomeric mixture of alpha and beta amyrin from Protium heptaphyllum (Aubl.) Marchand","authors":"G. Aragão, A. O. Nogueira, F. A. F. Xavier Júnior, Janaina Serra Azul Monteiro Evangelista, Paulo Nogueira Bandeira, Camila Fernandes, Maria Elisabete Amaral de Moraes, A. Assreuy","doi":"10.4025/actascibiolsci.v45i1.66144","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.66144","url":null,"abstract":"The isomeric mixture of alpha and beta amyrin (AMY), present in the resin of Protium heptaphyllum, is popularly used as anti-inflammatory and anti-ulcer. The literature has been demonstrating pharmacological activities of these triterpenes in the central and peripheral nervous systems, and in the gastrointestinal and immunological systems. This study traces a toxicological profile of amyrin, aiming to provide information that may clarify its safety. Nine female Wistar rats (170 to 200 g) were divided into three groups of three animals each (control, amyrin 300 and amyrin 2000 mg kg-1, p.o.), which were evaluated by protocols preconized by the Organization for Economic Co-operation and Development (OECD). Open field Test and Malone Hippocratic Screening Scale were performed. AMY, mostly at 2000 mg kg-1, reduced the number of crossings by 57% vs. saline (22.67 ± 2.40) and the number of rearing by 53% vs. saline (42.67 ± 2.96), but increased the number of grooming by 26% vs. saline (1.66 ± 0.33). AMY (2000 mg kg-1) increased the serum glucose by 77% vs. saline (126.70 ± 4.33 mg dL-1), triglycerides by 50% vs. saline (78.67 ± 2.18 mg dL-1) and uric acid by 65% vs. saline (0.73 ± 0.03 mg dL-1). AMY induced vascular congestion and hemorrhage in the liver, spleen and cerebral cortex. Renal changes (cellular damage, inflammatory infiltrate, tubular protein deposition and glomeruli atrophy) were also seen. In conclusion, AMY decreased rat locomotor activity, caused minor biochemical changes, and altered the morphology of the kidney. The present study may contribute to deepen the knowledge about the safety of AMY, aiming the development of a novel pharmacological product.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"251 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacteriological assessment of fruit pulp sold in supermarkets in São Raimundo das Mangabeiras, Maranhão State, Brazil 对巴西马拉尼昂州 São Raimundo das Mangabeiras 超市销售的果肉进行细菌学评估

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.67555

Kaio da Silva Bandeira, Tamires de Moura Lima, Yanna Mireya Passarinho Nogueira, Marcos Martins Lima, J. G. M. Rodrigues, Maurício Eduardo Chaves e Silva, G. S. Miranda

{"title":"Bacteriological assessment of fruit pulp sold in supermarkets in São Raimundo das Mangabeiras, Maranhão State, Brazil","authors":"Kaio da Silva Bandeira, Tamires de Moura Lima, Yanna Mireya Passarinho Nogueira, Marcos Martins Lima, J. G. M. Rodrigues, Maurício Eduardo Chaves e Silva, G. S. Miranda","doi":"10.4025/actascibiolsci.v45i1.67555","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.67555","url":null,"abstract":"Natural fruit pulp is highly appreciated because it retains the flavor and nutrients of the fresh fruit. However, fruit pulp is susceptible to contamination by pathogenic bacteria, mainly because of improper handling and storage. Therefore, this study evaluated and quantified the presence of pathogenic bacteria in fruit pulp sold in São Raimundo das Mangabeiras, Maranhão, Brazil. Two fruit pulp samples each of açaí (A and B), acerola (C and D), cajá (E and F), cashew (G and H), and passion fruit (I and J) were obtained from supermarkets and subjected to the following microbiological analyses: enumeration of mesophilic bacteria using plate count agar; quantification of total (TC) and thermotolerant (TTC) coliforms using the multiple tube technique; qualitative detection of Escherichia coli on eosin methylene blue agar and that of Salmonella on xylose lysine base agar and enteric agar. Mesophilic bacteria were found to grow in all the samples, with the highest value obtained for the acerola pulp (sample D; average: 1.08 x 105 colony-forming units g-1). However, all samples were in accordance with the Brazilian legislation for this parameter. The most probable number of colonies (MPN g-1) of TC and TTC were ≥1,100 in açaí (sample A), cajá (sample E), and cashew (sample G) pulps, which is in disagreement with the maximum value allowed. E. coli and Salmonella were not found to grow in the samples evaluated in this study. Collectively, the data indicate inadequate handling and storage in the production chain of fruit pulp sold in the municipality of São Raimundo das Mangabeiras, suggesting that the population in the region is exposed to a probable risk of food contamination.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungi and their medicinal properties: a scientometric study 真菌及其药用特性：科学计量学研究

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.63262

Isabelle Nucci Vetrone, Paula Santos, G. C. Mantovanelli, Fernando Emmanuel Gonçalves Vieira, D. Caetano, Bruno Rian Cintra Albino, T. Mantovano

{"title":"Fungi and their medicinal properties: a scientometric study","authors":"Isabelle Nucci Vetrone, Paula Santos, G. C. Mantovanelli, Fernando Emmanuel Gonçalves Vieira, D. Caetano, Bruno Rian Cintra Albino, T. Mantovano","doi":"10.4025/actascibiolsci.v45i1.63262","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.63262","url":null,"abstract":"Fungi are eukaryotic, heterotrophic, unicellular or multicellular organisms that feed by absorption. Some of their attributes stand out in the medicinal and pharmaceutical sector since several genera of fungi have metabolic properties and compounds related to the manufacture of numerous medicines. In this context, the objective of this study was to carry out a scientometric analysis to verify the application and properties of medicinal fungi as well as their contributions to the knowledge of medicinal fungi. A survey of applied literature, which included the word “Medicinal fungi”, was carried out based on the articles indexed in the Thomson Reuters base (www.isiwebofknowledge.com), considering the period from 2003 to 2021. One hundred and thirty-nine scientific articles were used for the scientometric analysis, and these were divided into six time intervals (2003-2006, 2007-2009, 2010-2012, 2013-2015, 2016-2018, 2019-2021). An increase was noted in the scientific production on medicinal fungi from 2007 onwards, mostly presenting publications with an experimental approach. The phylum Basidiomycota, followed by Ascomycota, presented the highest number of records in the study, mainly with applications aimed at antitumor and antibacterial activity. Therefore, the importance of studies that aim to expand the knowledge of the properties of medicinal fungi is highlighted, providing effective results for various diseases, since their cultivation and development can positively generate equitable economic growth, with regional and national impacts.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant response of Messastrum gracile (Chlorophyceae) to light-induced stress Messastrum gracile（叶绿藻）对光诱导胁迫的抗氧化反应

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.64996

Lúcia Helena Sipaúba Tavares, Mayara Cristina Malvas Nicolau, M. G. Tedesque, Priscila Lupina Gratão

{"title":"Antioxidant response of Messastrum gracile (Chlorophyceae) to light-induced stress","authors":"Lúcia Helena Sipaúba Tavares, Mayara Cristina Malvas Nicolau, M. G. Tedesque, Priscila Lupina Gratão","doi":"10.4025/actascibiolsci.v45i1.64996","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.64996","url":null,"abstract":"The antioxidant enzymes activities during the Messastrum gracile growth period under two different light intensities were evaluated. The light intensities were designated as: control at 60 µmol photons m-2 s-1 and two cultivations under light-induced stress at 120 µmol photons m-2 s-1, during 24 and 72 hours. The maximum cell density in all treatments was achieved on the 21st growth day with 140x105 cells mL-1 (control), 382x105 cells mL-1 (24 hours) and 236x105 cells mL-1 (72 hours). The antioxidant enzymes activities were more elevated in the control, however, GSH-Px, CAT and SOD activities were higher on the 7th growth day, while GPOX and GR activities were higher on the 28th and 21st growth day, respectively. The microalgae displayed lower MDA content, which indicates low stress levels. In terms of growth, biomass production and chlorophyll-a, the best results occurred under light-induced stress (120 µmol photons m-2 s-1). Light-induced stress decreased antioxidant activity during M. gracile growth, and the results revealed a higher activity for glutathione peroxidase, catalase and superoxide dismutase.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasites of juvenile Salminus brasiliensis and Brycon orbignyanus (Characiformes, Bryconidae) in the middle Paraná River, Argentina 阿根廷巴拉那河中游 Salminus brasiliensis 和 Brycon orbignyanus（鳐形目，Bryconidae）幼鱼的寄生虫

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.68282

Exequiel Oscar Furlan, S. Chemes, P. Scarabotti

{"title":"Parasites of juvenile Salminus brasiliensis and Brycon orbignyanus (Characiformes, Bryconidae) in the middle Paraná River, Argentina","authors":"Exequiel Oscar Furlan, S. Chemes, P. Scarabotti","doi":"10.4025/actascibiolsci.v45i1.68282","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.68282","url":null,"abstract":"Piscivorous Salminus brasiliensis and herbivorous/omnivorous Brycon orbignyanus often form mixed schools in the same habitats. The aims of this study were: (a) to analyze and compare ecto- and endoparasite communities in juvenile S. brasiliensis and B. orbignyanus in the Middle Paraná River, Argentina; and (b) to evaluate the possible relationships between degree of parasitic infestation and body condition (Kn) of the fish. Fish necropsy was performed in search of endo- and ectoparasites, which were mounted in permanent or semi-permanent preparations for taxonomic study using helminthological techniques. A total 24 macroparasite taxa were recorded from the two host species. The parasitic infracommunities of both hosts were competitively structured. Ectoparasite communities were more similar than endoparasite communities. The abundance of parasitic species was not correlated with the standard length, weight and Kn of host individuals, except for the abundance of the copepod E. lacusauratus in the host S. brasiliensis, which was positively related to fish condition. New records of parasites were observed for native hosts, extending the geographic range of several species already known in the Upper Paraná River. The general structure of parasite communities can be determined by several factors, such as parasite-host relationship, host trophic level, and environment. In concordance with the similarity in habitat preferences, and the strong differences in trophic preferences, ectoparasite communities were found to be more similar than endoparasite communities between the two host species.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel aspect of essential oils with a history of antimicrobial activity to be used as a potential source of carbon and nitrogen for the nutrition of Candida pathogens 具有抗菌活性的香精油可用作白色念珠菌病原体营养的潜在碳源和氮源的新方面

Acta Scientiarum. Biological Sciences Pub Date : 2023-10-27 DOI: 10.4025/actascibiolsci.v45i1.65589

Suad Aldorkee, A. A. H. S. AL-Janabi

引用次数: 0

Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae) 凤梨科细针莲(Nidularium minutum Mez)微繁过程中的差异发芽能力

Acta Scientiarum. Biological Sciences Pub Date : 2023-07-26 DOI: 10.4025/actascibiolsci.v45i1.64678

M. Teixeira, C. Nievola

{"title":"Differential sprouting ability during micropropagation of Nidularium minutum Mez (Bromeliaceae)","authors":"M. Teixeira, C. Nievola","doi":"10.4025/actascibiolsci.v45i1.64678","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.64678","url":null,"abstract":"Nidularium minutum is an ornamental bromeliad from the Brazilian Rainforest. The micropropagation of this species is essential for obtaining plants available for conservation programs or commercial use. Our study aimed to establish an efficient plant production method by in vitro sprouting. This bromeliad takes a long time to sprout in vitro, and 10% of the plants produce shoots in a culture medium without plant growth regulators (PGRs). When subcultured in a PGR-free medium, these individualized shoots can sprout like the mother plant. The Murashige and Skoog basal medium (MS) containing 1.0 mg L-1 of 6-benzylaminopurine (BAP) promoted the induction of adventitious shoots in greater than 90% of the plants after 240 days of culture with an average of more than eight shoots per plant. Approximately 100% of the in vitro-produced shoots survived after acclimatization, reaching the flowering stage. Therefore, our results showed that in vitro regeneration of N. minutum depends on the cultivation period and that plants with a higher sprouting capacity can be selected and used as micropropagation matrices, contributing to the production of this endangered bromeliad.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88440256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0