Acta Scientiarum. Biological Sciences最新文献

{"title":"Characterization of the digestive microbiome of invasive Limnoperna fortunei (Dunker, 1857) collected in different seasons in the upper Uruguay River, Santa Catarina, Brazil","authors":"T.H. Ferreira, L.S. Sá, Paula Brando de Mederios, Domickson Silva Costa, Grasiela Fagundes Minatto Cardoso, B.S. Pierri, A. Nuñer, M. Martins, J. L. P. Mouriño","doi":"10.4025/actascibiolsci.v46i1.69338","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v46i1.69338","url":null,"abstract":"We aimed to identify the digestive microbiota of the golden mussel Limnoperna fortunei, and characterize its structure in different seasons. Limnoperna fortunei specimens were sampled in winter (September 2019) and summer (March 2020) in five reservoirs of hydroelectric power plants in southern Brazil. In each reservoir, we sampled 15 individuals at three different points. We collected the digestive diverticulum and portions of the intestine for bacterial DNA extraction, and subsequently conducted metagenomic analysis. The intestinal microbial communities of L. fortunei occurring in the upper Uruguay River region showed significant differences during winter and summer. Although the phyla Proteobacteria, Bacteroidetes, and Cyanobacteria were dominant regardless of the season, the microbial communities showed greater richness and bacterial diversity in the summer. Additionally, we found 143 species of bacteria in the digestive samples collected during both winter and summer, which may indicate a central microbial community for the species. The microbial communities in the digestive tract of L. fortunei showed greater bacterial richness and diversity in summer samples, which existed at a significantly higher temperature than those in winter samples. We observed seasonal variations in the microbiota of L. fortunei in the upper Uruguay River region, with increased bacterial richness and diversity in the digestive tract during summer, attributed to higher temperatures. However, Proteobacteria, Bacteroidetes, and Cyanobacteria consistently dominated the digestive tract of the golden mussel, regardless of seasonal fluctuations.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141925959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity among genotypes of Parkia platycephala (Benth.), a typical tree of northeastern Brazil","authors":"Raiane de Sousa Oliveira, G. D. N. Costa, Raimundo Nonato Oliveira Silva, Paulo S.C. Lima, R. Araújo Neto, Angela CA Lopes, F. Martins","doi":"10.4025/actascibiolsci.v45i1.64168","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.64168","url":null,"abstract":"Parkia platycephala (Fabaceae) is a useful tree. The leaves are rich in protein, fiber and minerals and are good quality, low-cost fodder for animals, making it a great option for animal feed during the dry season in Northeast Brazil. In addition, wood is used in small buildings and as fuel. With the aim of exploring genetic variation within the species, we have assessed the molecular and morpho-agronomic characteristics of 10 accessions (F1 - F10) of the species originating from the same location and maintained in the Forage Collection at Embrapa Meio Norte. Clustering analyses based on the amplicons generated by 12 inter simple sequence repeat (ISSR) primers and on 36 qualitative/quantitative markers separated the accessions into two groups, the constituents of which were dependent on the characteristics considered. The most divergent genotypes according to ISSR analysis were F3 and F8. Morpho-agronomic analysis identified F2 and F7 as the most divergent, while the traits that contributed most (36.5%) to total diversity were, in order of importance, mature inflorescence length, stem length, immature inflorescence width, seed weight and pedicel length. The results revealed that there is sufficient genetic variability among the studied accessions. These accessions with greater diversity are candidates for actions that promote the conservation, domestication and genetic improvement of the species.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"533 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140453884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative investigation of bioactive compounds present in Datura stramonium L. leaves extract in various solvents (ethanol, aqua, and ether) using TLC, IR, and GC-MS analytical techniques","authors":"Vijayavilas Sathyan Sangeetha, Christina Ruby Stella","doi":"10.4025/actascibiolsci.v45i1.67455","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.67455","url":null,"abstract":"The present study is an experimental comparative investigation of the biomolecules present in ethanol, aqua and ether extract of Datura stramonium L. leaves. The purpose of the study is to monitor the phytochemical constituents in the plant leaves by thin layer chromatography (TLC), Gas Chromatography-Mass Spectrometry (GC-MS), and infrared spectroscopy analytical techniques. The Datura Stramonium L. leaves were collected, dried and was extracted with ethanol, water and ether at room temperature. A comparative investigation of bioactive compounds have been carried out using TLC, GC-MS, and IR Analytical techniques. Preliminary phytochemical analysis revealed the presence of protein, alkaloid, phenol and tannins, flavanoid, glycoside, sterols, saponin, resins, anthocyanin, and carboxylic acid. More number of bio active phytochemicals were identified, and the chromatograph showed peaks of individual compounds. The result of this study offers a platform for understanding the Phytochemical present in ethanol, aqua and ether extract of studied plant leaves and to identify them as herbal alternative for various diseases and it can be used as functional pharmaceutical when once it extracted and treated carefully.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"26 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140460100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bat fauna from the Sooretama Biological Reserve, southeastern Brazil","authors":"Carina Maria Vela-Ulian, J. P. M. Hoppe, A. Ditchfield","doi":"10.4025/actascibiolsci.v45i1.64721","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.64721","url":null,"abstract":"The Atlantic Forest is one of the world's hotspots of biodiversity, a heterogeneous biome encompassing tropical and subtropical vegetation formations. Its remnants in the state of Espírito Santo, southeastern Brazil, are mostly within state and federal Conservation Units. The largest Unit is the Sooretama Biological Reserve, which harbors several endemic occurrences of fauna and flora, and is the last refuge of some threatened animal species. Inventories of fauna are essential to know the community composition, and for the elaboration of preservation strategies. We conducted a survey focused on the bat fauna of the Sooretama Reserve, analyzing the trophic guilds and the conservation status of its species. We surveyed online taxonomic databases and the literature, resulting in 402 records from two different scientific collections. These records represent five families, 39 genera, and 57 species of bats, revealing that the Sooretama Biological Reserve has all known bat families in the Atlantic Forest biome. Phyllostomidae was the family with the highest number of species registered, and a majority of species belongs to the aerial insectivorous trophic guild. Four species are registered as “Vulnerable” in state and federal conservation books.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140459938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of effective microorganisms changed the soil microbiota of canals in Maricá city, Brazil","authors":"B.S. Pierri, Norha Constanza Bolívar Ramírez, Caio Bruno Nogueira de Paula Matos, Victor Aleluia da Silva, Charles Vieira Neves, E. Fonseca","doi":"10.4025/actascibiolsci.v45i1.67690","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.67690","url":null,"abstract":"The objective of this study was to evaluate the effect of bioremediation, through the application of two different strategies of effective microorganisms as treatment of effluents in the parameters of water quality and in the bacteriological profile of the soil of ‘Canal da Cidade’ - Maricá, Rio de Janeiro, Brazil. The metagenomic analyzes indicated that the abundance and richness of microorganisms in the substrate increased significantly after the bioremediation strategy used in this study. Because of bioremediation, 31 bacterial species disappeared from the environment when comparing the initial and final bacterial profiles of the soil, where 94% of these 31 species were anaerobic. Furthermore, 61 new aerobic or facultative aerobic species appeared in the channel substrate after bioremediation. As a consequence of bioremediation, the dissolved oxygen concentration and pH values increased significantly throughout the bioremediation process, indicating that the changes found in the microbiological profile of the soil contributed to the improvement of water quality parameters, helping the environment to move from anaerobic to aerobic characteristics. Thus, it is possible to state that the use of effective microorganisms directly affected the physical-chemical parameters of water quality and the microbial profile of the soil community of ‘Canal da Cidade’.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier","authors":"Angélica Vieira Aguiar, Lana Dorneles Pedroso, Bruna Trindade Borges, S. Vestena","doi":"10.4025/actascibiolsci.v45i1.65188","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.65188","url":null,"abstract":"The objective of this study was to evaluate the entomotoxic activity of the hydroalcoholic extract of Eragrostis plana Nees in an experimental model of cockroaches (Nauphoeta cinerea Olivier), for a possible bioinsecticide. For this purpose, aerial parts of E. plana were grounded to obtain the hydroalcoholic extract (EACA), after preparing the concentrations of (0.5; 2.5; 5.0; 12.5; 25.0; 50.0 100.0 μg g-1 per animal) a saline solution was used to control the treatment. To determine the receptors and pathways involved in responses to EACA doses of E. plana, a treatment with the drug octopamine and its inhibitor phentolamine were prepared. The effect of EACA on behavioral activities (grooming) and on cardiac activity were tested. All data were expressed as mean ± standard deviation, being analyzed by the two-way ANOVA test, followed by the Tukey test, considered significant when p<0.05. After carrying out the experiment, it was found that EACA induced a significant effect on leg grooming at all concentrations, except for the concentration of 100 μg g-1 per animal, but the same did not modulate antenna grooming. Regarding the receptors and pathways involved, it was observed that octopamine alone and together with the extract, had a significant effect on leg grooming, while phentolamine, together with the extract, influenced antennae grooming. Regarding the effect of the extract on the semi-isolated heart, it was noticed that the same doses that affected grooming, affected heart rate by decreasing it. At no concentration, at the end of the treatment, there was no recovery of the heart rate. The results point to a direct modulation of octopaminergic and cholinergic pathways in the insect nervous system.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Gulf toadfish goes south: assessing the potential habitat suitability of Opsanus beta in response to climate change","authors":"João Henrique Alliprandini da Costa, Ursulla Pereira Souza","doi":"10.4025/actascibiolsci.v45i1.68519","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.68519","url":null,"abstract":"Climate change can directly influence invasive species range, and may reflect in greater habitat suitability, consequently increasing losses in native biodiversity. Habitat suitability modelling can help identify priority areas for conservation and rapid response for invasive taxa. This study aimed to model the habitat suitability of Opsanus beta, an invasive fish species, in South America under current and future climate change scenarios. Three modelling techniques, Generalized Linear Model (GLM), Maximum Entropy Algorithm (Maxent), and Random Forest (RF), were applied, and their results were compared and then put together in an Ensemble model to visualize habitat suitability. We found that the current habitat suitability of O. beta in South America is relatively low, with its occurrences mostly related to coastal port areas, suggesting that the species was introduced from ballast water. The increase in mean temperature at mean depth and temperature range were the most relevant variables influencing the species habitat suitability. The gradual increase in habitat suitability for the 2100 climate change scenarios, particularly in more severe climate change scenarios, such as the RCP 8.5, was also observed. The study highlights the critical need to use habitat suitability models to mitigate the impact of future climate change scenarios on invasive species. The findings call for preventive measures to be taken in time to prepare areas that may become the target of dispersal and establishment of O. beta.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain","authors":"Barno Alimova, O. Pulatova, A. Makhsumkhanov, Lobar Khasanova, Kakhramon Davranov","doi":"10.4025/actascibiolsci.v45i1.65392","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.65392","url":null,"abstract":"This article provides a study of the molecular, physiological, biochemical and morphological features of R-, S- and M-dissociants of Rhodococcus ruber - 8/4/1. The sequence similarity of the genome fragments of R- and S-dissociant forms encoded by 16S rRNA was demonstrated. MALDI spectra were obtained for the R. ruber-8/4/1 strain and its dissociants. Under standard analysis conditions, the MALDI profiles of R-, S- and M- forms dissociants of the Rhodococcus ruber - 8/4/1 were similar. The determination of the resistance of the strain dissociants to external factors showed that R-forms were more stable under UV irradiation and increased temperature. The S-forms showed stability upon the conversion of acrylic acid nitrile into acrylamide (AA), whereas the M-forms were stable under a decrease in the cultivation temperature, and an increase in the concentration of NaCl in the medium. The S-forms proved to be the most active in the transformation of acrylonitrile into AA.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive advantage of Sesbania virgata (Cav.) Pers. in the phytochemical production: the influence on fungi occurring in seeds","authors":"Vera Lygia El Id Id, Nelson Augustos dos Santos Junior","doi":"10.4025/actascibiolsci.v45i1.65301","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.65301","url":null,"abstract":"Sesbania virgata is a native Brazilian plant species. It exhibits rapid growth, a high soil cover potential, and efficient soil seed bank formation and is used in environmental restoration projects. The soil seed bank is susceptible to fungal infection and other biotic factors. However, only a few studies have reported on the fungi on the surface of S. virgata seeds. Moreover, little is known about how substances present in the seed integument affect fungal communities and their role in adapting to and thriving in new environments. Herein, S. virgata seeds were collected from populations that produce or do not produce the flavonoid catechin in the seed coat. These seeds were subjected to laboratory tests to identify and quantify the fungal populations in the integument. We selected and subjected three genera to irrigation and inoculation tests with S. virgata extracts and seeds from both populations. We observed that the aqueous seed coat extracts inhibited Alternaria sp. micellar and augmented Phoma sp. growth. Phoma sp. also caused post-germinated seed lethality. Our data indicate that the seed coat of S. virgata contains antifungal substances that endow this species with an adaptive advantage.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139313073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The plant community of a stretch of riparian vegetation in the upper Paraná River after 20 years of secondary succession (Batayporã, Mato Grosso do Sul, Brazil)","authors":"Luiz Antonio Frazatto Fernandes, Kazue Kawakita, Alexandrina Pujals, Victoria Sotti Batista, M. Romagnolo","doi":"10.4025/actascibiolsci.v45i1.65557","DOIUrl":"https://doi.org/10.4025/actascibiolsci.v45i1.65557","url":null,"abstract":"The study of temporal changes in riparian vegetation is essential for understanding the paths taken by the ecosystem during ecological succession. In order to evaluate the changes in floristic composition, structure, and dynamics of the arboreal vegetation in an area of Semideciduous Seasonal Alluvial Forest, we aim to understand the secondary succession that occurs in the Paraná River floodplain. Based on a study conducted 20 years ago, in the municipality of Batayporã, Mato Grosso do Sul, a re-sampling was carried out in the same area, using the same methodology. Phytosociological and ecological parameters between the two temporal samplings were collected and compared using Student's ‘t’ test, where it was possible to find statistically significant variations (p<0.05) in the vegetation structure. A total of 603 individuals were sampled, distributed among 51 species and grouped in 46 genera and 31 families. The analysis of the distribution of abundance and importance values (IV) of species visualized in the histogram indicated an expected pattern in forest communities, which is the substitution of early successional species for late successional species. For the successional categories, the comparison of IVs revealed higher values for secondary species in both surveys and changes in the positions of climax and pioneer species, directly influencing the IVs values of these categories for the zones. When compared to the result obtained in the previous study, a replacement of the IV values of pioneer species with climax and dead species is noted. The comparisons showed that this phytocoenosis is evolving to a mature successional stage, a result of the decrease in the main anthropic impacts in the area. The results obtained highlight the importance of temporal monitoring of forest areas to expand the understanding of the pathways of ecological succession in this type of environment and provide information to assist in biodiversity conservation.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139312615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}