A comparative investigation of bioactive compounds present in Datura stramonium L. leaves extract in various solvents (ethanol, aqua, and ether) using TLC, IR, and GC-MS analytical techniques

Q3 Agricultural and Biological Sciences

Acta Scientiarum. Biological Sciences Pub Date : 2024-02-08 DOI:10.4025/actascibiolsci.v45i1.67455

Vijayavilas Sathyan Sangeetha, Christina Ruby Stella

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引用次数: 0

Abstract

The present study is an experimental comparative investigation of the biomolecules present in ethanol, aqua and ether extract of Datura stramonium L. leaves. The purpose of the study is to monitor the phytochemical constituents in the plant leaves by thin layer chromatography (TLC), Gas Chromatography-Mass Spectrometry (GC-MS), and infrared spectroscopy analytical techniques. The Datura Stramonium L. leaves were collected, dried and was extracted with ethanol, water and ether at room temperature. A comparative investigation of bioactive compounds have been carried out using TLC, GC-MS, and IR Analytical techniques. Preliminary phytochemical analysis revealed the presence of protein, alkaloid, phenol and tannins, flavanoid, glycoside, sterols, saponin, resins, anthocyanin, and carboxylic acid. More number of bio active phytochemicals were identified, and the chromatograph showed peaks of individual compounds. The result of this study offers a platform for understanding the Phytochemical present in ethanol, aqua and ether extract of studied plant leaves and to identify them as herbal alternative for various diseases and it can be used as functional pharmaceutical when once it extracted and treated carefully.

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利用 TLC、IR 和 GC-MS 分析技术，比较研究曼陀罗叶提取物在不同溶剂（乙醇、水和乙醚）中所含的生物活性化合物

本研究是对曼陀罗（Datura stramonium L.）叶片的乙醇、水和乙醚提取物中存在的生物大分子进行的实验性比较调查。研究的目的是通过薄层色谱法（TLC）、气相色谱-质谱法（GC-MS）和红外光谱分析技术监测植物叶片中的植物化学成分。采集曼陀罗花叶，干燥后在室温下用乙醇、水和乙醚提取。使用 TLC、GC-MS 和红外分析技术对生物活性化合物进行了比较研究。初步的植物化学分析显示了蛋白质、生物碱、酚和单宁、黄酮、苷、甾醇、皂苷、树脂、花青素和羧酸的存在。鉴定出了更多具有生物活性的植物化学物质，色谱仪显示出了单个化合物的峰值。这项研究的结果为了解所研究植物叶片的乙醇、水和乙醚提取物中的植物化学成分提供了一个平台，并可将其确定为治疗各种疾病的草药替代品，一旦提取并经过仔细处理，还可将其用作功能性药物。

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来源期刊

Acta Scientiarum. Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

38 weeks

期刊介绍： The journal publishes original articles in all areas of Biological Sciences, including anatomy, bacteriology, molecular biology, biochemistry, botany, cytology and cell biology, animal behavior, ecology, limnology, embryology, and histology, morpho-physiology, genetics, microbiology, parasitology and zoology.