Acta Chromatographica最新文献_第4页

Evaluation of various commercial lipid removal sorbents for the detection of 18 neonicotinoid insecticides and their metabolites in meat by liquid chromatography tandem mass spectrometry 液相色谱-串联质谱法检测肉类中18种新烟碱类杀虫剂及其代谢物的各种商用脱脂吸附剂的评价

4区化学

Acta Chromatographica Pub Date : 2023-10-03 DOI: 10.1556/1326.2023.01170

Kai Li, Liqiang Guo, Guoning Tian, Xiaojie Xu, Yajing Li

{"title":"Evaluation of various commercial lipid removal sorbents for the detection of 18 neonicotinoid insecticides and their metabolites in meat by liquid chromatography tandem mass spectrometry","authors":"Kai Li, Liqiang Guo, Guoning Tian, Xiaojie Xu, Yajing Li","doi":"10.1556/1326.2023.01170","DOIUrl":"https://doi.org/10.1556/1326.2023.01170","url":null,"abstract":"Abstract A highly accurate and precise method for the simultaneous detection of 18 neonicotinoids and their metabolites in meat was developed using liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). To improve the pretreatment step of the method, five different commercially available clean-up materials (including C18+PSA (primary secondary amine), Z-Sep (with Discover DSC-C18), EMR-Lipid, SHIMSEN QuEChERS, and Clean-up LPAS) were studied in the treatment of three meat matrices: pork, duck and yellow croaker. Based on the recovery data, we found that among the five purification materials, SHIMSEN QuEChERS was slightly more effective than the others for 18 neonicotinoids. Therefore, SHIMSEN QuEChERS was used as the purification sorbent, and the extraction solvents, extraction methods and chromatographic and mass spectrometric conditions were optimized. A matrix-matched calibration method was applied for quantification. In three different meat matrixes (pork, duck, and yellow croaker), all the target compounds showed good linearity, both with values of r 2 > 0.995. The average recovery of all neonicotinoids ranges from 63.4 to 114.2% (pork), 63.0–113.2% (duck), and 63.9–110.5% (yellow croaker). Relative standard deviations were all <15% for intraday and interday precision. The values of limit of detection (LOD) and limit of quantification (LOQ) were, respectively, ranging from 0.04 to 1.0 μg kg −1 and 0.10 to 2.0 μg kg −1 . Compared with previous reports, this method has advantage in LOQs, indicating that it it may be a preferred choice for the detection of neonicotinoid pesticides in meat samples.","PeriodicalId":7130,"journal":{"name":"Acta Chromatographica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135739029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-rapid determination of andrographolide and dehydroandrographolide in Andrographis Herba by solid phase extraction and liquid chromatography with mass spectrometry 固相萃取-液相色谱-质谱法超快速测定穿心莲中穿心莲内酯和脱水穿心莲苷

IF 1.9 4区化学

Acta Chromatographica Pub Date : 2023-09-04 DOI: 10.1556/1326.2023.01156

Zhengming Qian, J. Chen, Qing-Qing Lei, Da Li, Guoying Tan, Juying Xie, Wenqing Li

{"title":"Ultra-rapid determination of andrographolide and dehydroandrographolide in Andrographis Herba by solid phase extraction and liquid chromatography with mass spectrometry","authors":"Zhengming Qian, J. Chen, Qing-Qing Lei, Da Li, Guoying Tan, Juying Xie, Wenqing Li","doi":"10.1556/1326.2023.01156","DOIUrl":"https://doi.org/10.1556/1326.2023.01156","url":null,"abstract":"An ultra-rapid analytical method for determination of andrographolide and dehydroandrographolide in Andrographis Herba (AH) was developed by liquid chromatography with mass spectrometry (LC-MS). The sample was ultrasonically extracted with 10 mL 40% (v/v) methanol, and then purified with a C18 solid phase extraction column. The LC separation was performed on a Poroshell 120 EC-C18 column (30 × 2.1 mm, 2.7 μm) and eluted with 0.5 mmol L−1 ammonium acetate aqueous solution and acetonitrile (65:35) at a flow rate of 0.7 mL min−1, and detected by mass spectrometry (MS). The LC-MS analytical time was less than 1 min. The new developed method presented a good linearity (r > 0.9900), precision and repeatability (RSD < 2.0%). The recoveries for andrographolide and dehydroandrographolide were 93.5% (RSD = 2.2%) and 97.7% (RSD = 2.4%), respectively. The developed method was successfully applied in determination of andrographolide and dehydroandrographolide in seven batches of AH samples, and the contents of analytes in all samples were complied with the relative acceptance criteria in Chinese Pharmacopeia (>0.8%). This new developed LC-MS method is an ultra-rapid assay method for AH, which will help to improve the efficiency and reduce the cost of AH sample test.","PeriodicalId":7130,"journal":{"name":"Acta Chromatographica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49366115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The use of an RP-HPLC-UV method for the analysis of oxcarbazepine in the presence of its preservatives; stability studies and application to human plasma samples 用反相高效液相色谱-紫外分光光度法分析奥卡西平中防腐剂的含量稳定性研究及其在人血浆样品中的应用

IF 1.9 4区化学

Acta Chromatographica Pub Date : 2023-09-01 DOI: 10.1556/1326.2023.01157

A. Fouad, Ahmed S Abdelkhalek, Hisham Elrefay, Hany A. Batakoushy, M. K. Soltan

{"title":"The use of an RP-HPLC-UV method for the analysis of oxcarbazepine in the presence of its preservatives; stability studies and application to human plasma samples","authors":"A. Fouad, Ahmed S Abdelkhalek, Hisham Elrefay, Hany A. Batakoushy, M. K. Soltan","doi":"10.1556/1326.2023.01157","DOIUrl":"https://doi.org/10.1556/1326.2023.01157","url":null,"abstract":"A simple, sensitive, selective, accurate and precise method was developed and fully validated for determination of oxcarbazepine (OXC) in presence of their preservatives and determination of oxcarbazepine (OXC) in human plasma. A reversed phase liquid chromatography (RP-HPLC) with UV detection techniques were applied for separation and quantification of studied drug OXC. Successful separation of the drug from methyl paraben (M.P.), propyl paraben (P.P.) and potassium sorbate (P.ST.) was achieved on a Kromasil C18 column (5 μm particle size, pore size 300 Å, l × I.D. 250 × 4.6 mm). The mobile phase that contain aqueous 0.05M potassium dihydrogen phosphate buffer (pH 7): acetonitrile, (50: 50, %v/v). The method was linear over concentration ranges 5.0–50 μg mL−1 for OXC. Bioanalytical validation of the developed method was carried out according to US-FDA guidelines and revealed a good linear relations over a range of (5.0–50), (0.5–10), (0.05–0.15), and (1.0–10) μg mL−1 for OXC, M.P, P.P, and P.ST, respectively, with a correlation coefficient (R2) of more than 0.999. Limit of detection (LOD) were 1.15, 0.03, 0.01 and 0.04 μg mL−1 for OXC, M.P, P.P, and P.ST, respectively, Intra and inter-day precisions, calculated as percentage relative standard deviation (% RSD), were lower than 2.0%. The developed method can be applied for routine drug analysis, therapeutic drug monitoring and bioequivalence studies through the analysis of plasma samples taken from blood bank.","PeriodicalId":7130,"journal":{"name":"Acta Chromatographica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46859556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new approach for determination of urinary 8-hydroxy-2′-deoxyguanosine in cancer patients using reinforced solid/liquid phase microextraction combined with HPLC-DAD 强化固/液相微萃取联合HPLC-DAD测定癌症患者尿中8-羟基-2′-脱氧鸟苷的新方法

IF 1.9 4区化学

Acta Chromatographica Pub Date : 2023-07-04 DOI: 10.1556/1326.2023.01142

Nabil N. Al-Hashimi, Rand O. Shahin, A. El-Sheikh, Malak Jibreel, Nada Alsakhen, A. Alqudah, Muna Oqal, Jafar I. Abdelghani

{"title":"A new approach for determination of urinary 8-hydroxy-2′-deoxyguanosine in cancer patients using reinforced solid/liquid phase microextraction combined with HPLC-DAD","authors":"Nabil N. Al-Hashimi, Rand O. Shahin, A. El-Sheikh, Malak Jibreel, Nada Alsakhen, A. Alqudah, Muna Oqal, Jafar I. Abdelghani","doi":"10.1556/1326.2023.01142","DOIUrl":"https://doi.org/10.1556/1326.2023.01142","url":null,"abstract":"The concentration level of urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), an oxidative stress biomarker for various diseases especially cancer, has been attracted as a pathway suitable for diagnostic purposes. Determination of urinary 8-OHdG is challenging due to its low level within a complex matrix. In this study, a new approach of solid/liquid phase microextraction technique prior to high-performance liquid chromatography diode-array detection (HPLC-DAD) analysis was developed for the determination of trace levels of 8-OHdG in urine samples. The solid/liquid phase microextraction device was constructed by reinforcement of multi-walled carbon nanotubes into the pores of a short segment 2.5 cm of hollow fiber microtube with two ends heat sealed. Based on the optimized procedure, the selected analyte was extracted from an acidic sample solution (10 mL adjusted at pH = 5) into the five extraction devices. After the extraction period (30 min), the 8-OHdG was eluted from the extraction device using methanol (350 µL) under ultrasonication for 5 min. The analytical performance of the method in synthetic urine samples showed good linearity (R2 > 0.999) with the limits of detection of 0.85 ng mL−1, and extraction recovery > 92.36%. The developed microextraction technique exhibited a confident sensitivity, feasible operation, and simplicity in comparison with other published methods and was valid to determinate trace 8-OHdG in urine cancer patients' samples by using a cheap and commonly available HPLC-DAD instrument.","PeriodicalId":7130,"journal":{"name":"Acta Chromatographica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49007615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Residues of difenoconazole in various ready premixes with propiconazole, cyflufenamid, and mandipropamid in/on tomato fruits 苯醚甲环唑与丙环唑、西氟芬胺和曼地丙酰胺在番茄果实中的残留

IF 1.9 4区化学

Acta Chromatographica Pub Date : 2023-06-15 DOI: 10.1556/1326.2023.01134

Osama I. Abdallah, N. S. Ahmed, Rania M. Abd El-Hamid, S. Alhewairini

{"title":"Residues of difenoconazole in various ready premixes with propiconazole, cyflufenamid, and mandipropamid in/on tomato fruits","authors":"Osama I. Abdallah, N. S. Ahmed, Rania M. Abd El-Hamid, S. Alhewairini","doi":"10.1556/1326.2023.01134","DOIUrl":"https://doi.org/10.1556/1326.2023.01134","url":null,"abstract":"Residues of the fungicides difenoconazole, propiconazole, cyflufenamid, and mandipropamid were determined in tomato fruit using acetonitrile for extraction and LC-MS/MS for quantification. Validation criteria include linearity range, the limit of detection (LOD) and limit of quantitation (LOQ), accuracy in terms of precision and trueness, and matrix effect were studied. The recovery rates of the method ranged from 91.8 to 106.3%. The precision of the method in terms of repeatability at one day (RSDr) and between three days (RSDR) ranged from 2.8 to 6.4% and from 4.3 to 7.6%, respectively, with good trueness from 92.2 to 96.4%. Matrix effects (suppression effects) ranged from 3.8% to 11.1%. The validated method was used to evaluate the dissipation kinetics of three different premix formulations: 30% EC (15% difenoconazole + 15% propiconazole), 14% DC (12.5% difenoconazole + 1.5% cyflufenamid), and 50% SC (25% difenoconazole + 25% mandipropamid) used on field tomatoes in Egypt. A first-order kinetic equation best describes residue dissipation. The calculated half-lives of difenoconazole, propiconazole, cyflufenamid, and mandipropamid were 2.01–2.27, 1.89, 1.97, and 1.71 days, respectively. The dissipation rate of difenoconazole did not differ significantly in the three premix formulations. Mandipropamid also dissipated faster compared to the other fungicides tested. The chronic dietary risk assessment results showed a minimal risk to adult Egyptian consumers. Waiting periods were advised for the safe consumption of tomatoes treated with the tested premix formulations.","PeriodicalId":7130,"journal":{"name":"Acta Chromatographica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45732570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In memoriam Professor Dr Teresa Kowalska (1946–2023) 纪念特蕾莎·科瓦尔斯卡教授(1946-2023)

IF 1.9 4区化学

Acta Chromatographica Pub Date : 2023-05-17 DOI: 10.1556/1326.2023.11111

引用次数: 0

Development and validation of an LC-MS/MS method for the quantification of free and total doxorubicin in human plasma and its clinical application for a novel doxorubicin hydrochloride liposome injection in Chinese patients with breast cancer LC-MS/MS定量人血浆游离和总阿霉素方法的建立与验证及新型盐酸阿霉素脂质体注射液在中国乳腺癌患者中的临床应用

IF 1.9 4区化学

Acta Chromatographica Pub Date : 2023-05-09 DOI: 10.1556/1326.2023.01129

Keyu Zhang, Daqing Zhang, Z. Ge, F. Qiu

{"title":"Development and validation of an LC-MS/MS method for the quantification of free and total doxorubicin in human plasma and its clinical application for a novel doxorubicin hydrochloride liposome injection in Chinese patients with breast cancer","authors":"Keyu Zhang, Daqing Zhang, Z. Ge, F. Qiu","doi":"10.1556/1326.2023.01129","DOIUrl":"https://doi.org/10.1556/1326.2023.01129","url":null,"abstract":"A novel doxorubicin hydrochloride liposome injection was prepared to reduce toxicity and side effects, as well as extend plasma half-life in the treatment of breast cancer. In this study, a rapid and sensitive bioanalytical method was developed and validated to characterize the pharmacokinetic profile of total and free doxorubicin in plasma of 3 Chinese patients after intravenous infusion of this injection. Plasma samples were prepared by protein precipitation for the determination of total doxorubicin, while solid phase extraction was used to determine free doxorubicin. After plasma sample pre-treatment, total and free concentrations were quantified individually using a validated LC-MS/MS method. The calibration curves were found to be linear in the range of 0.20–500.0 ng mL−1 for total doxorubicin and in the range of 1.00–1,000 ng mL−1 for free doxorubicin. The free concentrations in plasma were only one sixth to one quarter of the total levels. Liposomal doxorubicin had a longer apparent half-life (>50 h) than the non-targeted drug (<10 h) reported in the reference. and a lower volume of distribution. This novel injectable formulation steadily released free doxorubicin from liposomes over a long period of time to reduce cardiac toxicity and side effects, while ensuring a clinical curative effect.","PeriodicalId":7130,"journal":{"name":"Acta Chromatographica","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2023-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42950221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: High performance thin layer chromatography (HPTLC) method development and validation for the simultaneous determination of paracetamol, caffeine, chlorpheniramine and phenylepherine in tablet formulation 高效薄层色谱(HPTLC)同时测定片剂中扑热息痛、咖啡因、氯苯那敏和苯肾上腺素的方法的建立和验证

4区化学

Acta Chromatographica Pub Date : 2023-04-25 DOI: 10.1556/1326.2022.11028

Almaz Arage, Thomas Layloff, Ariaya Hymete, Ayenew Ashenef

引用次数: 0

Rapid comparison of antitumor chemical constituents and mechanisms between Dendrobium nobile and Dendrobium officinale by UPLC-IT-TOF, network pharmacology and experimental verification 利用UPLC-IT-TOF、网络药理学和实验验证快速比较铁皮石斛和铁皮石斛抗肿瘤化学成分及其作用机制

4区化学

Acta Chromatographica Pub Date : 2023-04-25 DOI: 10.1556/1326.2022.01012

Jie Liu, Zhaoyang Li, Meiling Zeng, Yan Liang, Bing Liang, Qiuping Ge, Xiaoyan Zhang, Shilin Zhang, Wei Zhou

{"title":"Rapid comparison of antitumor chemical constituents and mechanisms between Dendrobium nobile and Dendrobium officinale by UPLC-IT-TOF, network pharmacology and experimental verification","authors":"Jie Liu, Zhaoyang Li, Meiling Zeng, Yan Liang, Bing Liang, Qiuping Ge, Xiaoyan Zhang, Shilin Zhang, Wei Zhou","doi":"10.1556/1326.2022.01012","DOIUrl":"https://doi.org/10.1556/1326.2022.01012","url":null,"abstract":"Abstract Dendrobium nobile and Dendrobium officinale as the main varieties of traditional Chinese medicine Dendrobium are widely used in clinic. The study aimed to systematically explore chemical constituents and their antitumor effect of D. nobile and D. officinale by ultra-performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UPLC-IT-TOF), network pharmacology and cancer cell experiments. D. nobile extract and D. officinale extract could significantly inhibit the proliferation of human lung cancer A549 cells, human liver cancer HepG2 cells and human breast cancer MCF-7 cells in the dose-dependent manner ( P < 0.05), the antitumor effect of D. officinale extract was stronger than that of D. nobile extract at the same drug concentration. A total of 40 chemical constituents of D. nobile and D. officinale including phenanthrenes, bibenzyls and other types of compounds had been identified by UPLC-IT-TOF, LCMSsolution and MetID software according to retention times, accurate mass, MS n fragmentation, reference compounds and natural product databases. Phenanthrenes with good antitumor activity were mainly present in D. nobile , bibenzyls were the main compounds of D. officinale . Integrated networks of Herb-Compounds-Targets-Cancer revealed that gigantol, moscatilin, tristin, moscatin and densiflorol B were regarded as key antitumor compounds of D. nobile and D. officinale , D. nobile and D. officinale shared 7 targets accounting for 70% of the antitumor core targets, more than half of their antitumor KEGG pathways were similar. The results of molecular docking and western blotting experiments indicated that the antitumor mechanisms of D. nobile and D. officinale may be through inhibiting PI3K-Akt and HIF-1α signaling pathways.","PeriodicalId":7130,"journal":{"name":"Acta Chromatographica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135066138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of morphine, codeine, thebaine, papaverine and noscapine in rat plasma by UPLC-MS/MS UPLC-MS/MS法测定大鼠血浆中吗啡、可待因、拜因、罂粟碱和诺斯卡平的含量

IF 1.9 4区化学

Acta Chromatographica Pub Date : 2023-04-19 DOI: 10.1556/1326.2023.01108

Ying Wang, Yifan He, Xianqin Wang, Congcong Wen, Jianbin Cao

引用次数: 0