Acta microbiologica et immunologica Hungarica最新文献_第3页

Epidemiological and bacterial profile of childhood meningitis in Tunisia. 突尼斯儿童脑膜炎的流行病学和细菌概况。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-05-21 Print Date: 2024-07-02 DOI: 10.1556/030.2024.02208

Sarra Dhraief, Khaoula Meftah, Samar Mhimdi, Houyem Khiari, Firas Aloui, Aida Borgi, Sondes Haddad-Boubaker, Amani Brik, Khaled Menif, Amel Kechrid, Aida Bouafsoun, Hanen Smaoui

{"title":"Epidemiological and bacterial profile of childhood meningitis in Tunisia.","authors":"Sarra Dhraief, Khaoula Meftah, Samar Mhimdi, Houyem Khiari, Firas Aloui, Aida Borgi, Sondes Haddad-Boubaker, Amani Brik, Khaled Menif, Amel Kechrid, Aida Bouafsoun, Hanen Smaoui","doi":"10.1556/030.2024.02208","DOIUrl":"10.1556/030.2024.02208","url":null,"abstract":"The worldwide burden of disease of bacterial meningitis remains high, despite the decreasing incidence following introduction of routine vaccination campaigns.The aim of our study was to evaluate the epidemiological and bacteriological profile of paediatric bacterial meningitis (BM) in Tunisian children, during the period 2003-2019, following the implementation of Haemophilus influenzae type b (Hib) vaccine (April 2011) and before 10-valent pneumoccocal conjugate vaccine (PCV10) introduction to the childhood immunization program.All bacteriologically confirmed cases of BM admitted to children's hospital of Tunis were recorded (January 2003 to April 2019). Serogroups of Neisseria meningitidis (Nm) and serotypes of Streptococcus pneumoniae (Sp) and H. influenzae (Hi) and antibiotic resistance were determined using conventional and molecular methods.Among 388 cases, the most frequent species were Sp (51.3%), followed by Nm (27.5%) and Hi (16.8%). We observed a significant decrease in Hi BM rate during the conjugated Hib vaccine use period (P < 0.0001). The main pneumococcal serotypes were 14, 19F, 6B, 23F and 19A and the serotype coverage of PCV10, PCV13, PCV15 and PCV20 was 71.3 and 78.8%, 79.4 and 81.9% respectively. The most frequent Nm serogroup was B (83.1%). Most Hi strains were of serotype b (86.9%). High levels of resistance were found: Sp and Nm to penicillin (respectively 60.1 and 80%) and Hi to ampicillin (42.6%). All meningococcal and Hi isolates were susceptible to third-generation cephalosporins and 7.2% of pneumococcal strains had decreased susceptibility to these antibiotics.The Hib conjugate vaccine decreased the rate of BM. Sp dominated the aetiology of BM in children in Tunisia. Conjugate vaccines introducing decreases not only BM cases but also antimicrobial resistance.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the emergence of SARS-CoV-2 JN.1 sub-variant: Insights from the first cases at Charles Nicolle Hospital, Tunisia. 揭示 SARS-CoV-2 JN.1 亚变种的出现：突尼斯 Charles Nicolle 医院首例病例的启示。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-05-08 Print Date: 2024-07-02 DOI: 10.1556/030.2024.02247

Zaineb Hamzaoui, Sana Ferjani, Lamia Kanzari, Roua Ben Ali, Latifa Charaa, Ichrak Landolsi, Ines Medini, Sarra Chammam, Salma Abid, Asma Ferjani, Dhouha Kebaier, Ahmed Fakhfakh, Zoubeir Bouslah, Mouna Ben Sassi, Sameh Trabelsi, Ilhem Boutiba-Ben Boubaker

{"title":"Unveiling the emergence of SARS-CoV-2 JN.1 sub-variant: Insights from the first cases at Charles Nicolle Hospital, Tunisia.","authors":"Zaineb Hamzaoui, Sana Ferjani, Lamia Kanzari, Roua Ben Ali, Latifa Charaa, Ichrak Landolsi, Ines Medini, Sarra Chammam, Salma Abid, Asma Ferjani, Dhouha Kebaier, Ahmed Fakhfakh, Zoubeir Bouslah, Mouna Ben Sassi, Sameh Trabelsi, Ilhem Boutiba-Ben Boubaker","doi":"10.1556/030.2024.02247","DOIUrl":"10.1556/030.2024.02247","url":null,"abstract":"The JN.1 sub-variant is a new variant of the SARS-CoV-2 Omicron strain, derived from the BA.2.86 sub-variant. It was first detected in late 2023 and has quickly spread to many countries, becoming the most prevalent variant in some regions. JN.1 exhibits a unique mutation (L455S) in the spike protein compared to the BA.2.86 lineage, which may affect its transmissibility and immune evasion capabilities. JN.1 has been designated as a \"variant of interest\" by the World Health Organization due to its rapidly increasing spread and is being closely monitored for its impact on the COVID-19 pandemic. This study describes the emergence of SARS-CoV-2 JN.1 sub-variant in Tunisia, and reports its mutation profiles.Nasopharyngeal samples collected over a four-month period (October 2023 to January 2024) were subjected to RNA extraction and real-time RT-PCR confirmation of SARS-CoV-2 infection. The whole-genome sequencing was performed by an iSeq 100 sequencer and COVIDSeq kit reagents (Illumina, USA).Mutation analysis, using the NextClade platform and GISAID database, revealed the presence of JN.1 in 15 out of 80 positive cases (18.75%) during the study period.The emergence of JN.1 highlights the ongoing evolution of SARS-CoV-2 and the need for continued surveillance and research to better understand the characteristics and impact of emerging variants.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140891054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and genetic characteristics of Staphylococcus aureus isolates from cell phones of medical students from Iran. 从伊朗医学生手机中分离出的金黄色葡萄球菌的流行率和遗传特征。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-04-15 DOI: 10.1556/030.2024.02193

Fatemeh Behrouzmanesh, Sahar Ahmad Samali, Rozhin Nasehi, Ali Shivaee, Mehdi Goudarzi

{"title":"Prevalence and genetic characteristics of Staphylococcus aureus isolates from cell phones of medical students from Iran.","authors":"Fatemeh Behrouzmanesh, Sahar Ahmad Samali, Rozhin Nasehi, Ali Shivaee, Mehdi Goudarzi","doi":"10.1556/030.2024.02193","DOIUrl":"https://doi.org/10.1556/030.2024.02193","url":null,"abstract":"Although mobile phones as a rapid communication vehicle can lead to improved quality of healthcare, they can also facilitate the transmission of pathogens to patients. This current research focuses on genetic diversity, and genes involved in resistance and biofilm production of Staphylococcus aureus isolates from mobile phones of medical students. Antibiotic resistance profiling and polymerase chain reaction (PCR) amplification of antibiotic resistance and biofilm-related genes were investigated and statistically analyzed. Staphylococcal cassette chromosome mec (SCCmec) types were analyzed by multiplex PCR, and S. aureus protein A gene typing (spa typing) was done using PCR and sequencing. Sixty-four S. aureus isolates (16.8%) were obtained from 380 medical students' mobile phones who were working in hospitals. The findings showed that 71.9% of the isolates were MRSA and 78.1% were classified as MDR. All isolates exhibited sensitivity to vancomycin and linezolid. Overall, 7.8% of the isolates displayed an inducible clindamycin resistance phenotype, while 26.7% showed resistance to mupirocin. The results indicated that 68.8% of the isolates were biofilm producers, with 7 isolates (15.9%) classified as strong producers, 22 isolates (50%) as moderate producers, and 15 isolates (34.1%) as weak producers. The most prevalent type was CC8-MRSA III/t030 (18.7%), followed by CC8-MRSA III/t037 (12.5%), CC/ST22-MSSA/t790 (10.9%), CC1-MRSA IV-t114 (9.4%), CC1-MRSA IV-t127 (7.8%), CC8-MRSA V/t064 (7.8%), CC/ST15-MSSA-t360 (7.8%), CC30-MSSA/t021(6.3%), MRSA V-t355 (6.3%), CC8-MRSA III/t421 (4.7%), CC1-MRSA V-t267 (4.7%), and CC/ST15-MSSA-t084 (3.1%). The genetic diversity and prevalent multidrug resistance indicate that the resistance situation of S. aureus recovered from mobile phones in Tehran is severe, posing a potential threat to patients, the community, and healthcare settings.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140583313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

16S rRNA sequencing reveals microbiota differences in orthopedic implants between aseptic loosening and prosthetic joint infection cases. 16S rRNA 测序揭示了无菌性松动和人工关节感染病例中骨科植入物微生物群的差异。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-04-15 DOI: 10.1556/030.2024.02265

Omer Faruk Cetiner, Mehmet Hora, Ibrahim Halil Kafadar, Aysegul Ulu Kilic, Aycan Gundogdu

{"title":"16S rRNA sequencing reveals microbiota differences in orthopedic implants between aseptic loosening and prosthetic joint infection cases.","authors":"Omer Faruk Cetiner, Mehmet Hora, Ibrahim Halil Kafadar, Aysegul Ulu Kilic, Aycan Gundogdu","doi":"10.1556/030.2024.02265","DOIUrl":"https://doi.org/10.1556/030.2024.02265","url":null,"abstract":"Prosthetic joint infection (PJI) and aseptic loosening (AL) are common complications of total joint arthroplasty. An accumulation of evidence indicates the presence of microbial communities on prosthetic implants, but the overall microbial profile is unclear. In this study, we aimed to investigate the differences in the microbial composition of prosthetic implants obtained from PJI and AL patients using the 16S rRNA sequencing method. Patients who underwent revision hip, knee, or shoulder arthroplasty caused by PJI (n = 20) or AL (n = 10) were enrolled in the study. 16S rRNA sequencing targeting the V3-V4 region was performed on the microbial specimens collected from synovial fluid, periprosthetic deep-tissue, and biofilm during the revision surgery. The sequenced raw data were analysed for microbial composition and ecological and differential abundance analyses using bioinformatics tools. The AL group had relatively balanced and higher diversity, with Staphylococcus, Streptococcus, and Veillonella being prominent. In the PJI group, Staphylococcus and Pseudomonas were predominant, especially in deep-tissue samples and biofilm samples, respectively. The differential abundance analysis identified 15 and 2 distinctive taxa in the AL and PJI groups, respectively. Our findings provided preliminary insights supporting the existence of periprosthetic microbiota in orthopedic implants and explaining the differences in microbial composition between the AL and PJI groups.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of carbapenemase-producing gram-negative bacteria directly from blood culture bottles for a reliable guided empiric therapy. 直接从血液培养瓶中快速检测产碳青霉烯酶的革兰氏阴性菌，为经验疗法提供可靠的指导。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-04-04 DOI: 10.1556/030.2024.02237

Shahinda Rezk, Nourhan Ashraf, Hassan Heshmat, Gamal Elsawaf, Amel Elsheredy

{"title":"Rapid detection of carbapenemase-producing gram-negative bacteria directly from blood culture bottles for a reliable guided empiric therapy.","authors":"Shahinda Rezk, Nourhan Ashraf, Hassan Heshmat, Gamal Elsawaf, Amel Elsheredy","doi":"10.1556/030.2024.02237","DOIUrl":"https://doi.org/10.1556/030.2024.02237","url":null,"abstract":"Bloodstream infections (BSIs) caused by multidrug-resistant bacteria are a critical life-threatening challenge which necessitates the urgency to trigger life-saving treatment in a timely manner. This study aimed to evaluate the time required for rapid detection of carbapenemase-producing Enterobacterales (CPE) directly from blood culture bottles to optimize empirical treatment of BSI, especially in pediatric and infant patients, using a cost-effective method. This study included 419 Gram-negative bacteria, of which Klebsiella pneumoniae and Escherichia coli were the most common CPE causing BSI in pediatric and neonatal patients. Phenotypic and genotypic resistance of the selected isolates (45 K. pneumoniae and 9 E. coli) were determined by VITEK-2 Compact system and PCR, respectively. BACT/ALERT bottles were spiked with isolates. Finally, colorimetric RESIST-BC assay and Vitek-2 compact system were evaluated for the rapid detection of carbapenem-resistant bacteria directly from positive blood culture bottles. All selected isolates were phenotypically resistant to carbapenems. PCR showed that blaNDM and blaOXA-48 were present in all isolates, blaVIM was present in 44.4%, while blaKPC and blaIMP were entirely absent. The RESIST-BC kit showed good agreement with PCR for blaNDM and blaOXA-48, demonstrating high sensitivity and specificity, but not with blaVIM. These findings point out that RESIST-BC assay demonstrated an exceptionally short detection time for CPE, completing all cases within the first hour after the blood culture bottles flagged positive. It is also superior in providing a clue for clinicians on antibiotic combinations that can be administered, depending on the type of β-lactamases detected, promptly and efficiently, with low expenses.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents. 从人类和啮齿动物中分离出的大利什曼原虫的系统发育分析和抗锑能力。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-03-21 Print Date: 2024-03-26 DOI: 10.1556/030.2024.02194

Yussef Moghaddam, Hajar Ziaei Hezarjaribi, Abdol Sattar Pagheh, Mahdi Fakhar, Reza Saberi, Mitra Sharbatkhori, Mahbobeh Montazeri, Hossein Ghalehnoei, Eisa Nazar

{"title":"Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents.","authors":"Yussef Moghaddam, Hajar Ziaei Hezarjaribi, Abdol Sattar Pagheh, Mahdi Fakhar, Reza Saberi, Mitra Sharbatkhori, Mahbobeh Montazeri, Hossein Ghalehnoei, Eisa Nazar","doi":"10.1556/030.2024.02194","DOIUrl":"10.1556/030.2024.02194","url":null,"abstract":"Cutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. Clinical isolates and rodent samples were subjected to MTT assay to determine IC50 values and in vitro susceptibilities. Expression levels of antimony resistance-related genes were determined in CL isolates. Out of 1,949 suspected patients with CL and 148 rodents, 1,704 (87.4%) and 6 (4.05%) were positive with direct smear, respectively. Digestion patterns of BusRI (HaeIII) endonuclease enzyme were similar to what expected for Leishmania major. Phylogenetic analysis revealed that the highest interspecies similarity was found between current L. major sequences with L. major obtained from Russia and Uzbekistan. Out of 20 L. major samples tested, 13 (65%) were resistant to meglumine antimoniate (MA) treatment, with an activity index (AI) exceeding 4. The remaining 7 samples (35%) responded to MA treatment and were classified as sensitive isolates, with a confirmed sensitive phenotype based on their AI values. The comparison expression analysis of three major antimony resistance-associated genes in unresponsive clinical isolates demonstrated significant fold changes for TDR1 (4.78-fold), AQP1 (1.3-fold), and γ-GCS (1.17-fold) genes (P < 0.05). Herein, we demonstrate genetic diversity and antimony resistance of L. major isolated from human and reservoir hosts in north-eastern Iran, which could be the basis for planning future control strategies.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140193055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occupational exposure to Coxiella burnetii during cardiac surgery: A case report and review of the literature. 心脏手术中的职业性烧伤柯西氏菌暴露：病例报告和文献综述。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-03-11 Print Date: 2024-03-26 DOI: 10.1556/030.2024.02240

Patient Diwunga, Matthieu Million, Isabelle Ravaux, Pierre Edouard Fournier, Philippe Gautret

{"title":"Occupational exposure to Coxiella burnetii during cardiac surgery: A case report and review of the literature.","authors":"Patient Diwunga, Matthieu Million, Isabelle Ravaux, Pierre Edouard Fournier, Philippe Gautret","doi":"10.1556/030.2024.02240","DOIUrl":"10.1556/030.2024.02240","url":null,"abstract":"We report a case of exposure to Coxiella burnetii in a surgical nurse who underwent an injury of her finger with a scalpel blade during a native aortic valve replacement with a bio-prosthetic cardiac valve conducted on a patient suffering from C. burnetii aortic endocarditis. Given the positivity of C. burnetii culture and PCR from the patient's aortic valve, she was prescribed prophylactic doxycycline 100 mg twice a day for 10 days. Q fever is an occupational zoonosis resulting usually of exposure to infected animals by inhalation of infected aerosols or consumption of contaminated raw milk. Apart from materno-foetal transmission, about 180 cases of human-to-human C. burnetii transmission have been published from 1949 to today, including transmission by blood transfusion, sexual relations, transmission in the healthcare setting to staff, patient attendants and other patients that were likely infected from inhalation of aerosol from respiratory or placental products, transmission to staff during autopsies of patients with Q fever and transmission in familial settings. As C. burnetii is a highly infectious bacterium, that may cause infection with a low inoculum, it should be added to the list of organisms which may be of concern following blood exposure among healthcare professionals.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140100717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KPC-2 and VIM-1 producing Klebsiella pneumoniae ST39 high-risk clone isolated from a clinical sample in Volos, Greece. 从希腊沃洛斯的一份临床样本中分离出的产生 KPC-2 和 VIM-1 的肺炎克雷伯氏菌 ST39 高危克隆。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-03-07 Print Date: 2024-03-26 DOI: 10.1556/030.2024.02226

Maria Chatzidimitriou, Pandora Tsolakidou, Chatzivasileiou Panagiota, Eleni Mylona, Stella Mitka

{"title":"KPC-2 and VIM-1 producing Klebsiella pneumoniae ST39 high-risk clone isolated from a clinical sample in Volos, Greece.","authors":"Maria Chatzidimitriou, Pandora Tsolakidou, Chatzivasileiou Panagiota, Eleni Mylona, Stella Mitka","doi":"10.1556/030.2024.02226","DOIUrl":"10.1556/030.2024.02226","url":null,"abstract":"Klebsiella pneumoniae is a major human pathogen, because it causes both community- and hospital-acquired infections. Several multidrug-resistant high-risk clones of K. pneumoniae have been reported worldwide, and these are responsible for high numbers of difficult-to-treat infections. In Greece, a K. pneumoniae ST39 high-risk clone was detected in 2019 in a survey of carbapenem- and/or colistin-resistant Enterobacteriacae. The present study included nine carbapenem-resistant K. pneumoniae (CRKP) isolates collected during a retrospective analysis from October 2020 to December 2020. They were isolated from nine different patients hospitalized in the intensive care unit (ICU) of a hospital in Volos, Greece, and they were selected for analysis due to their phenotypic profile. In this study, we analyzed A165 strain K. pneumoniae ST39 isolated from a blood culture in November 2020. Whole-genome sequencing (WGS) was performed using Ion Torrent Platform, and resistance genes, virulence determinants, capsular types, insertion sequences, phage regions, and clustered regularly interspaced palindromic repeats (CRISPR) regions were detected by bioinformatic analysis. The molecular characterization revealed antimicrobial resistance genes, including sul2 for sulfamethoxazole; dfrA1 for trimethoprim; blaVIM-1 and blaKPC-2 for carbapenems; aac(6')-II for aminoglycosides; fosA for fosfomycin and aad1 for streptomycin, blaSHV-40, blaSHV-85, blaSHV-79, blaSHV-56, and blaSHV-89 for beta-lactams. Point mutations were identified in ompK36, and ompK37 and in acrR, gyrA, parC. Several replicons were found, including CoIRNA, IncC, IncFIB(K), IncFIB(pQiL), and IncFII(K). The capsular typing revealed that the strain was KL23, O2afg. The genome sequence of A165 was submitted to NCBI under PRJNA1074377 and have been assigned to Genbank accession number JAZIBV000000000.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140048528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbapenem-resistant Klebsiella pneumoniae in the Balkans: Clonal distribution and associated resistance determinants. 巴尔干地区耐碳青霉烯类肺炎克雷伯氏菌：克隆分布及相关耐药性决定因素。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-02-21 Print Date: 2024-03-26 DOI: 10.1556/030.2024.02230

Maria Chatzidimitriou, Asimoula Kavvada, Dimitrios Kavvadas, Maria Anna Kyriazidi, Konstantinos Eleftheriadis, Sotirios Varlamis, Vassilis Papaliagkas, Stella Mitka

{"title":"Carbapenem-resistant Klebsiella pneumoniae in the Balkans: Clonal distribution and associated resistance determinants.","authors":"Maria Chatzidimitriou, Asimoula Kavvada, Dimitrios Kavvadas, Maria Anna Kyriazidi, Konstantinos Eleftheriadis, Sotirios Varlamis, Vassilis Papaliagkas, Stella Mitka","doi":"10.1556/030.2024.02230","DOIUrl":"10.1556/030.2024.02230","url":null,"abstract":"Carbapenems are considered to be among the last line antibiotics against extended-spectrum β-lactamase producing Enterobacterales. Carbapenem-resistant Klebsiella pneumoniae (CRKP) has been frequently reported and its spread in Europe is indisputable and poses an enormous threat to hospitalized patients which is of growing concern. This review aims to record prevalence of CRKP in the Balkan region and to review the current knowledge about this life-threatening pathogen. In this review, we summarize data about clinical isolates of carbapenem-resistant K. pneumoniae from Greece, Croatia, Romania, Bulgaria, Serbia, Slovenia, Montenegro, Bosnia-Herzegovina and Albania from published reports between 2000 and 2023. Among Balkan countries, Greece and Romania are the ones with the most reports about CRKP. Since 2007, KPCs are the dominant carbapenemases in both countries. KPC-2 and NDM-1-producing K. pneumoniae strains have been identified as the most frequent CRKP in Croatia, Bulgaria, Serbia, and Slovenia. OXA-48 enzyme has been identified in most Balkan countries. In addition, since 2018, CRKP sequence type 11 (ST11) seems to have replaced ST258 in Balkan Peninsula, while ST15 continues to thrive throughout the years. Not only efficacy of colistin against CRKP has decreased dramatically during the last ten years but colistin resistance mechanism is based on alterations of chromosomal mgrB gene, rather than the already known mcr genes.Moreover, ceftazidime-avibactam-resistant CRKP were detected mostly in Greece. Emergence of CRKP poses a severe threat to the Balkan countries. Due to the narrow therapeutic window, it is essential to prevent the spread of multiresistant K. pneumoniae strains.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High rates of intestinal colonization with carbapenemase producing Enterobacteriaceae in hematopoietic stem cell transplant recipients. 造血干细胞移植受者肠道中产碳青霉烯酶肠杆菌科细菌的高定植率。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-02-21 Print Date: 2024-03-26 DOI: 10.1556/030.2024.02210

Ikram Ayari, Yosra Chebbi, Anis Raddaoui, Dorra Belloumi, Siwar Frigui, Rim Werhni, Tarek Ben Othmen, Nour Abedejlil, Wafa Achour

{"title":"High rates of intestinal colonization with carbapenemase producing Enterobacteriaceae in hematopoietic stem cell transplant recipients.","authors":"Ikram Ayari, Yosra Chebbi, Anis Raddaoui, Dorra Belloumi, Siwar Frigui, Rim Werhni, Tarek Ben Othmen, Nour Abedejlil, Wafa Achour","doi":"10.1556/030.2024.02210","DOIUrl":"10.1556/030.2024.02210","url":null,"abstract":"Carbapenem resistant Enterobacteriaceae (CRE) are major human pathogens because, these cause high number of difficult-to-treat infections. Allogeneic hematopoietic stem cell transplant (AHSCT) recipients are highly exposed to these type of bacteria. The aim of our study was to investigate prevalence of CRE colonization in AHSCT patients and to determine genes encoding carbapenem resistance. A retrospective study conducted between January 2015 and December 2019, involved 55 patients colonized with CRE strains. We determined the rate of antibiotic resistance according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the carbapenem resistance genes by PCR assays for genes encoding most frequent β-lactamases namely, blaGES, blaKPC, blaIMI, blaNDM, blaVIM, blaIMP and blaOXA-48. Eighty-one episodes of CRE colonization were recorded in 55 patients, mainly suffering from acute leukaemia (30%) and aplastic anemia (26%). History of hospitalization was noted in 80 episodes. Prior antibiotic treatment, severe neutropenia and corticosteroid therapy were respectively found in 94%, 76% and 58% of cases. Among the 55 patients, six patients (11%) developed a CRE infection. The CRE responsible for colonization were carbapenemase producers in 90% of cases. They belonged mostly to Klebsiella pneumoniae (61/81) and Escherichia coli species (10/81). Antibiotic resistance rates were 100% for ertapenem, 53% for imipenem, 42% for amikacin, 88% for ciprofloxacin and 27% for fosfomycin. Molecular study showed that blaOXA-48 gene was the most frequent (60.5%), followed by blaNDM (58%). Thirty-five (43%) strains were co-producers of carbapenemases. In our study, we report a high rate of CRE intestinal colonization in AHSCT recipients of our center.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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