Acta microbiologica et immunologica Hungarica最新文献_第4页

HCV genotype distribution in Istanbul: A detailed 7 year epidemiological overview and impact of Covid-19 pandemic. 伊斯坦布尔的 HCV 基因型分布：7 年流行病学详细概述及 Covid-19 大流行的影响。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-10-21 Print Date: 2024-12-19 DOI: 10.1556/030.2024.02388

Begüm Nalça Erdin, Yüksel Akkaya, Arzu İrvem

{"title":"HCV genotype distribution in Istanbul: A detailed 7 year epidemiological overview and impact of Covid-19 pandemic.","authors":"Begüm Nalça Erdin, Yüksel Akkaya, Arzu İrvem","doi":"10.1556/030.2024.02388","DOIUrl":"10.1556/030.2024.02388","url":null,"abstract":"Hepatitis C virus (HCV) is a leading cause of chronic liver disease, cirrhosis, and hepatocellular carcinoma worldwide. HCV has 8 genotypes (GT) and 86 subtypes and distribution of GTs varies based on geographical regions, transmission routes and even in cultural groups. The determination of viral genotype is crucial in choosing antiviral treatment, determining the duration of therapy, and monitoring treatment respose. Since 2014, with the usage of direct-acting antiviral agents (DAAs) in the treatment of HCV infections, a cure rate over 95% could be possible. Epidemiological data are important to combat a chronic HCV infections. Due to its geographical location, Turkey is like a bridge connecting Asia and Europe. Istanbul is the biggest and most crowded city of Turkey and has received immigration from many different countries, especially from Syria, in recent years and immigration still goes on. In addition, the COVID-19 pandemic has had devastating effects in our country. In this study, we determined the HCV genotypes in Health Sciences University Ümraniye Training and Research Hospital, in Istanbul between 2016 and 2022. Of the 322 patients analyzed during this 7-year period, HCV GT1b was the most prevalent GT in 65.2%, followed by GT3 in 15.5%, GT1a in 10.6%. Our data serve as a great mirror for HCV epidemiology in Turkey and contribute to global data.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"329-334"},"PeriodicalIF":1.3,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of KPC-3 producing Escherichia coli ST410 in Volos, Greece. 在希腊沃洛斯检测到产 KPC-3 的大肠杆菌 ST410。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-09-17 DOI: 10.1556/030.2024.02376

Maria Chatzidimitriou,Pandora Tsolakidou,Maria Anna Kyriazidi,Stella Mitka

引用次数: 0

Antibiotic resistant Escherichia coli strains in healthy pets from Tamaulipas, Mexico. 墨西哥塔毛利帕斯州健康宠物中的抗生素耐药大肠杆菌菌株。

IF 1.5 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-09-12 DOI: 10.1556/030.2024.02340

José Antonio Mandujano-Hernández,José Vázquez-Villanueva,Erick de Jesús De Luna-Santillana,Gildardo Rivera,Virgilio Bocanegra-García,Ana Verónica Martínez-Vázquez

{"title":"Antibiotic resistant Escherichia coli strains in healthy pets from Tamaulipas, Mexico.","authors":"José Antonio Mandujano-Hernández,José Vázquez-Villanueva,Erick de Jesús De Luna-Santillana,Gildardo Rivera,Virgilio Bocanegra-García,Ana Verónica Martínez-Vázquez","doi":"10.1556/030.2024.02340","DOIUrl":"https://doi.org/10.1556/030.2024.02340","url":null,"abstract":"Antibiotic resistance constitutes a significant public health challenge, with diverse reservoirs of resistant bacteria playing pivotal roles in their dissemination. Among these reservoirs, pets are carrying antibiotic-resistant strains. The objective of this study was to assess the resistance profiles of Escherichia coli, and the prevalence of extended-spectrum β-lactamase (ESBL) producing E. coli strains in dogs and cats from Tamaulipas, Mexico. A total of 300 stool samples (150 dogs and 150 cats) from healthy pets were subjected to analysis. Antibiotic susceptibility testing and the identification of ESBLs were carried out by disc diffusion method. The presence of resistance genes, class 1, 2, and 3 integrons (intI1, intI2, and intI3) and phylogroups was determined by PCR analysis. The findings reveal that 42.6% (128/300) of the strains exhibited resistance to at least one of the eight antibiotics assessed, and 18.6% (56/300) demonstrated multidrug resistance (MDR), that distributed across 69 distinct resistance patterns. Altogether 2.6% of E. coli strains (8/300) were confirmed as TEM and CTX-M type ESBL producers. These outcomes underscore the roles of dogs and cats in Tamaulipas as reservoirs for the dissemination of MDR and/or ESBL strains. The results underscore the necessity for conducting prevalence studies on ESBL-producing E. coli, forming a foundation for comprehending the present scenario and formulating strategies for the control and mitigation of this issue.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":"95 1","pages":"228-236"},"PeriodicalIF":1.5,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142263751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and characteristics of ESBL-producing Salmonella in Weifang, China. 中国潍坊产 ESBL 沙门氏菌的流行情况和特征。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-09-03 Print Date: 2024-09-18 DOI: 10.1556/030.2024.02347

Xuesong Wang, Binglei Wang, Xiaohong Lu, Jie Ma, Zhanzhao Wang, Yuhui Wang

{"title":"Prevalence and characteristics of ESBL-producing Salmonella in Weifang, China.","authors":"Xuesong Wang, Binglei Wang, Xiaohong Lu, Jie Ma, Zhanzhao Wang, Yuhui Wang","doi":"10.1556/030.2024.02347","DOIUrl":"10.1556/030.2024.02347","url":null,"abstract":"This study examined the prevalence and antibiotic resistance pattern of blaCTX-M extended-spectrum β-lactamase positive Salmonella species isolated from a hospital in Weifang. Salmonella strains were isolated from hospitalized patients from January 2018 to April 2023. Whole-genome sequencing was performed by Illumina platform. CTX-M-producing Salmonella were identified by Comprehensive Antibiotic Research Database (CARD). Strain susceptibility to six antimicrobial agents was assessed by BD Phoenix™ M50 System. MLST analysis confirmed sequence types and additionally, serotypes were determined by SeqSero2. Genetic environments of blaCTX-M genes were analyzed by Isfinder and BLASTn. Single nucleotide polymorphisms were used to construct a phylogenetic tree to analyze homology. A total of 34 CTX-M-producing Salmonella were detected. The most prevalent serotype was Salmonella enterica subsp. enterica 1,4,[5],12:i:- (14/34, 41.18%), belonging to ST34, followed by Salmonella Enteritidis (10/34, 29.41%), belonging to ST11. The highest resistance rate was detected to ampicillin (97.06%), followed by ceftriaxone (94.12%) and ceftazidime (58.83%). In CTX-M-producing Salmonella five types of blaCTX-M genes were identified, the most prevalent was blaCTX-M-55 (47.06%, 16/34), followed by blaCTX-M-14, blaCTX-M-65, blaCTX-M-125, and blaCTX-M-27 at 26.47% (9/34), 11.77% (4/34), 8.82% (3/34), and 5.88% (2/34), respectively. Apart from blaCTX-M, 40 antibiotic resistance genes were also detected, conveying resistance to multiple drugs and the most frequent genes were namely, mcr-1.1, aph(6)-Id, aph(3″)-Ib, oqxAB, qnrB6, qnrS1. According to genetic environment analysis, the insertion sequence ISEcp1 was prevalent upstream of the blaCTX-M gene. Our study demonstrates that multiple resistance genes are carried by clinical isolates of Salmonella spp. however, the dominant ESBL genotype is CTX-M-55, that is associated with ISEcp1.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"220-227"},"PeriodicalIF":1.3,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outbreak caused by multidrug-resistant OXA-48 and NDM-1 producing Klebsiella pneumoniae in the intensive care unit of a cancer hospital. 一家癌症医院重症监护室爆发由产生耐多药 OXA-48 和 NDM-1 的肺炎克雷伯菌引起的疫情。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-08-29 Print Date: 2024-09-18 DOI: 10.1556/030.2024.02364

Serap Süzük Yıldız, Esra Tavukcu, Sevgi Şahin, Gülşen İskender, Özlem Ünaldı, Buket Demirhan, Zuhal Avşar, Elif Özgür Orman, İpek Mumcuoğlu, Tuba Dal

{"title":"Outbreak caused by multidrug-resistant OXA-48 and NDM-1 producing Klebsiella pneumoniae in the intensive care unit of a cancer hospital.","authors":"Serap Süzük Yıldız, Esra Tavukcu, Sevgi Şahin, Gülşen İskender, Özlem Ünaldı, Buket Demirhan, Zuhal Avşar, Elif Özgür Orman, İpek Mumcuoğlu, Tuba Dal","doi":"10.1556/030.2024.02364","DOIUrl":"10.1556/030.2024.02364","url":null,"abstract":"We report a nosocomial outbreak caused by a multidrug-resistant carbapenemase-producing Klebsiella pneumoniae (MDRCPKp), that was detected in six patients admitted to the medical intensive care unit between 20th of December 2023 and 15th of January 2024 in Ankara, Turkey. The investigation of this outbreak was started on 29th of December 2023. During the outbreak 11 samples were collected from the six patients with MDRCPKp. Pulsed-field gel electrophoresis (PFGE) was performed to determine the genetic relatedness and clonality of MDRCPKp strains. MDRCPKp was isolated in the tracheal aspiration culture, blood, urine, and screening samples. Five patients with MDRCPKp colonization developed healthcare-associated infection. In one patient MDRCPKp was isolated from tracheal aspirate and the screening cultures were considered as colonization not infection. PFGE analysis revealed that all isolates belonged to the same K. pneumoniae clone. MDRCPKp strain of this outbreak exhibited multidrug resistance and co-produced OXA-48 and NDM-1. This outbreak ended after application of strict infection control measures. An outbreak of MDRCPKp can occur in hospitals, especially in the intensive care units; thus, it should be detected early by infection control teams. A strong collaboration between infection control team and microbiology laboratory is essential to cope with MDR bacterial outbreaks in hospitals.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"206-210"},"PeriodicalIF":1.3,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ESKAPE pathogens in pediatric cardiac surgery patients: 5-year microbiological monitoring in a tertiary hospital in Kazakhstan. 小儿心脏手术患者中的 ESKAPE 病原体：哈萨克斯坦一家三级医院的 5 年微生物监测。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-08-27 Print Date: 2024-09-18 DOI: 10.1556/030.2024.02352

Abay Baigenzhin, Nelya Bissenova, Aigerim Yergaliyeva, Shukhrat Marassulov, Elmira Tuleubayeva, Ulzhan Aitysheva

{"title":"ESKAPE pathogens in pediatric cardiac surgery patients: 5-year microbiological monitoring in a tertiary hospital in Kazakhstan.","authors":"Abay Baigenzhin, Nelya Bissenova, Aigerim Yergaliyeva, Shukhrat Marassulov, Elmira Tuleubayeva, Ulzhan Aitysheva","doi":"10.1556/030.2024.02352","DOIUrl":"10.1556/030.2024.02352","url":null,"abstract":"Hospital acquired infections greatly affect recovery and survival in pediatric surgical patients. We evaluated prevalence and antimicrobial resistance of ESKAPE pathogens in neonates and infants subjected to cardiac surgery in a tertiary hospital in Central Kazakhstan between 2019 and 2023 (2,278 patients) using routine methods of microbiological detection. ESKAPE pathogens were found in 1,899 out of 2,957 samples (Staphylococcus aureus - 35.3%, Klebsiella pneumoniae - 27.8%, Acinetobacter baumannii - 14.5%, Pseudomonas aeruginosa - 12.4%, Enterobacter sp. - 8.8%, Enterococcus faecium - 1.2%). The total prevalence of ESKAPE increased significantly from 45.1 to 76.9% (P = 0.005) during the study period. The resistance significantly increased in methicillin-resistant S. aureus (MRSA, from 13.7 to 41.9%, P = 0.041) but decreased in carbapenem-resistant P. aeruginosa (from 64.3 to 37.7%, P = 0.037) and carbapenem-resistant A. baumannii (from 48.5 to 19.1%, P = 0.039). Gradual but non-significant changes were shown in third-generation cephalosporin resistant K. pneumoniae (from 63.6 to 45.2%) and carbapenem-resistant K. pneumoniae (from 0 to 8.3%). The relative prevalence of ESKAPE pathogens steadily increased in our pediatric cardiac surgery patients in 2019-2023. The most frequent were S. aureus, K. pneumoniae, and A. baumannii, with dramatically increasing tendencies for MRSA. Our results highlight the necessity for a well-designed infection control strategy and constant microbiological monitoring in pediatric cardiac surgery departments.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"211-219"},"PeriodicalIF":1.3,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142078742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case report of an abdominal wet gangrene caused by Acidaminococcus. 由酸性球菌引起的腹部湿性坏疽病例报告。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-08-26 Print Date: 2024-09-18 DOI: 10.1556/030.2024.02332

Ahmed Fakhfakh, Sana Ferjani, Lamia Kanzari, Asma Ferjani, Zaineb Hamzaoui, Amel Rehaiem, Anis Ben Dhaou, Montassar Aloui, Yasser Karoui, Noura Syala, Hedia Bouaani, Mounir Ben Moussa, Ilhem Boutiba Ben Boubaker

{"title":"Case report of an abdominal wet gangrene caused by Acidaminococcus.","authors":"Ahmed Fakhfakh, Sana Ferjani, Lamia Kanzari, Asma Ferjani, Zaineb Hamzaoui, Amel Rehaiem, Anis Ben Dhaou, Montassar Aloui, Yasser Karoui, Noura Syala, Hedia Bouaani, Mounir Ben Moussa, Ilhem Boutiba Ben Boubaker","doi":"10.1556/030.2024.02332","DOIUrl":"10.1556/030.2024.02332","url":null,"abstract":"The Acidaminococcus genus is a part of the normal flora in gastrointestinal tract. It is a strictly anaerob Gram-negative coccus that is rarely pathogenic. We report the case of a 58-year-old man, who presented to surgery department A of the Charles Nicolle hospital, complaining of a wide inflammatory lesion in the anterior abdominal wall evolving for two weeks. Patient's anamnestic data included smoking, hypertension, and diabetes mellitus with poor compliance. The patient underwent flattening with excision of necrotic tissues and surgical drainage using a DELBET blade. Empirical antibiotic therapy with imipenem 1gx3/d, teicoplanin 400 mg 1 inj x2/d and gentamicin 400 mg 1 inj/d was administered pending bacteriological results. The bacteriological examination of a sample of necrotic tissue, after 72 h of incubation at 37 °C in anaerobic atmosphere, was able to detect a Gram-negative coccus, that the VITEK2 ANC system identified as Actinomyces canis with an accuracy of 80%. Whole genome sequencing was subsequently performed, that identified Acidaminococcus sp. AM33-14BH and demonstrated the following resistance genes: cfxa, tet(X) and tet(Q). An antibiogram for anaerobes was performed showing that the strain was resistant to amoxicillin but sensitive to amoxicillin-clavulanic acid, piperacillin-tazobactam, ertapenem, imipenem, meropenem and rifampin. Patient's condition improved after treatment with imipenem for 2 weeks, followed by oral amoxicillin-clavulanic acid for 16 days.This work highlights the role of molecular biology in the diagnosis of infections caused by anaerobes. Although the Vitek 2 ANC card provides rapid and acceptable identification of the most common anaerobic bacteria, improvements are needed for the identification of bacteria in the genera Acidaminococcus and Actinomyces.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"248-252"},"PeriodicalIF":1.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic characterization of an uncommon Delftia acidovorans isolate obtained from a Bulgarian immunocompetent outpatient diagnosed with bronchitis. 从保加利亚一名被诊断为支气管炎的免疫功能正常的门诊病人身上分离出的一种不常见的 Delftia acidovorans 的基因组特征。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-08-26 Print Date: 2024-09-18 DOI: 10.1556/030.2024.02331

Slavil Peykov, Raina Gergova, Svetlana Atanasova, Svetoslav G Dimov, Tanya Strateva

{"title":"Genomic characterization of an uncommon Delftia acidovorans isolate obtained from a Bulgarian immunocompetent outpatient diagnosed with bronchitis.","authors":"Slavil Peykov, Raina Gergova, Svetlana Atanasova, Svetoslav G Dimov, Tanya Strateva","doi":"10.1556/030.2024.02331","DOIUrl":"10.1556/030.2024.02331","url":null,"abstract":"Delftia acidovorans is an aerobic, non-fermenting Gram-negative bacterium (NFGNB), found in soil, water and hospital environments. It is rarely clinically significant, most commonly affecting hospitalized or immunocompromised patients. The present study aimed to explore the genomic characteristics of a Bulgarian clinical D. acidovorans isolate (designated Dac759) in comparison to all strains of this species with available genomes in the NCBI Genome database (n = 34). Dac759 was obtained in 2021 from the sputum of a 65-year-old female immunocompetent outpatient with bronchitis. Species identification using MALDI-TOF mass spectrometry, antimicrobial susceptibility testing, whole-genome sequencing (WGS), and phylogenomic analysis were performed. The isolate demonstrated high-level resistance to colistin (16 mg L-1); resistance to gentamicin; reduced susceptibility to piperacillin, piperacillin-tazobactam, ceftazidime, cefepime, ciprofloxacin, and levofloxacin; and susceptibility to imipenem, meropenem, amikacin, and tobramycin. The observed genome size (6.43 Mb) and GC content (66.76%) were comparable with the accessible data from sequenced D. acidovorans genomes. A limited number of resistance determinants were identified in the assembled genome as follows: blaOXA-459, emrE, oqxB, and mexCD-oprJ. The phylogenomic analysis indicated a high heterogenicity of the included D. acidovorans genomes. In conclusion, to the best of our knowledge, this is the first documented case of a clinically relevant D. acidovorans isolate in Bulgaria. Unlike the majority of reports in the literature, Dac759 affected a patient with no malignancies or other preexisting comorbidities. With this in mind, its genome sequence is a valuable resource for the fundamental study of uncommon bacterial pathogens of public health importance.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"242-247"},"PeriodicalIF":1.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the vaginal microbiota in infertile women with repeated implantation failure. 反复植入失败的不孕妇女的阴道微生物群特征。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-08-23 Print Date: 2024-09-18 DOI: 10.1556/030.2024.02323

Lidan Liu, Tong Feng, Qingyou Liu, Ming Liao, Bo Liu, Mujun Li

{"title":"Characterization of the vaginal microbiota in infertile women with repeated implantation failure.","authors":"Lidan Liu, Tong Feng, Qingyou Liu, Ming Liao, Bo Liu, Mujun Li","doi":"10.1556/030.2024.02323","DOIUrl":"10.1556/030.2024.02323","url":null,"abstract":"Although the relationship between vaginal microorganisms and fertility has been well established, only few studies have investigated vaginal microorganisms in women undergoing in vitro fertilization (IVF). Our aim was to study the differences in vaginal microbiota between infertile women with repeated implantation failure (RIF) and those who achieved clinical pregnancy in their first frozen embryo transfer cycle. We compared the vaginal microbiota of patients with a history of RIF (n = 37) with that of the control group (n = 43). Following DNA extraction, metagenomic sequencing was employed for the analysis of alpha and beta diversities, distinctions in bacterial species, and the functional annotation of microbial genes. Furthermore, disparities between the two groups were revealed. Alpha diversity analysis revealed that the Shannon index was higher in the RIF group (P < 0.05). There were differences in the beta diversity between groups (P = 0.16). At the bacterial family level, the relative abundance of Actinomycetaceae (P = 0.013) and Ruminococcaceae (P = 0.013) were significantly higher in the RIF group. At the genus level, the abundances of Actinomyces (P = 0.028) and Subdoligranulum (P = 0.013) were significantly higher in the RIF group. At the species level, the abundances of Prevotella timonensis (P = 0.028), Lactobacillus jensenii (P = 0.049), and Subdoligranulum (P = 0.013) were significantly higher in the RIF group. Significant differences in family, genus, species, alpha and beta diversity were observed in the vaginal microbiota between groups. Notably, among these findings, the Subdoligranulum genus emerged as the most prominent correlating factor.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"263-271"},"PeriodicalIF":1.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142043808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial resistance of Serratia marcescens causing blood stream infections in a large University Hospital in Bulgaria, an 8-year analysis (2016-2023). 保加利亚一所大型大学医院中引起血流感染的肉豆蔻沙雷氏菌的抗菌药耐药性，8 年分析（2016-2023 年）。

IF 1.3 4区医学

Acta microbiologica et immunologica Hungarica Pub Date : 2024-08-01 Print Date: 2024-09-18 DOI: 10.1556/030.2024.02330

Stephanie Radeva, Denis Niyazi, Milena Bozhkova, Temenuga Stoeva

{"title":"Antimicrobial resistance of Serratia marcescens causing blood stream infections in a large University Hospital in Bulgaria, an 8-year analysis (2016-2023).","authors":"Stephanie Radeva, Denis Niyazi, Milena Bozhkova, Temenuga Stoeva","doi":"10.1556/030.2024.02330","DOIUrl":"10.1556/030.2024.02330","url":null,"abstract":"The aim of this study is to evaluate the antimicrobial susceptibility of invasive isolates of Serratia marcescens, associated with blood stream infections (BSIs) in patients hospitalized in Varna University Hospital, Bulgaria, as well as to identify the genetic mechanisms responsible for 3rd generation cephalosporin and carbapenem-resistance among these isolates. A total of 45 consecutive S. marcescens isolates, obtained from blood cultures of 45 patients with BSIs, hospitalized during an 8-year period (2016-2023) were included. Species identification and antimicrobial susceptibility testing were done by Phoenix (BD, USA) and Vitek 2 (BioMerieux, France) systems and the results were interpreted according to EUCAST guidelines. The genetic mechanisms of beta-lactam resistance were studied by PCR. During the study period, a total of 45 patients were diagnosed with S. marcescens-associated BSIs. All infections were defined as nosocomial, predominantly intensive care unit-acquired (42.2%) and 28.8% were central venous catheter-associated. The following antimicrobial resistance rates were found: ceftriaxone, piperacillin/tazobactam, 57.8%; ceftazidime, 55.6%; cefepime, trimethoprime/sulfamethoxazole, 53.3%; gentamicin, 48.8%; ciprofloxacin, 44.5%; amikacin, 15.6%; carbapenems, 2.2%. The blaCTX-M was identified in 88.9% of the tested 3rd generation cephalosporin resistant isolates. Among these, 50% were also blaTEM positive. The single carbapenem-resistant isolate harboured blaKPC, blaCTX-M1/9, blaCMY-2 and blaTEM. This study demonstrates S. marcescens as a problematic nosocomial pathogen and we report a KPC-producing S. marcescens clinical isolate from a BSI in Bulgaria.","PeriodicalId":7119,"journal":{"name":"Acta microbiologica et immunologica Hungarica","volume":" ","pages":"191-196"},"PeriodicalIF":1.3,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141873913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0