Acta Neuropathologica最新文献

{"title":"MN1 immunohistochemistry is a sensitive diagnostic biomarker for primitive CNS tumors with MN1 fusion","authors":"Roxane Daniel, Arnault Tauziède-Espariat, Alice Métais, Charlotte Berthaud, Noémie Pucelle, Joelle Lacombe, Aurélien Collard, Fabrice Chrétien, Pascale Varlet","doi":"10.1007/s00401-024-02827-6","DOIUrl":"10.1007/s00401-024-02827-6","url":null,"abstract":"","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02827-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raphe and ventrolateral medulla proteomics in sudden unexplained death in childhood with febrile seizure history","authors":"Dominique F. Leitner,&nbsp;Christopher William,&nbsp;Arline Faustin,&nbsp;Evgeny Kanshin,&nbsp;Matija Snuderl,&nbsp;Declan McGuone,&nbsp;Thomas Wisniewski,&nbsp;Beatrix Ueberheide,&nbsp;Laura Gould,&nbsp;Orrin Devinsky","doi":"10.1007/s00401-024-02832-9","DOIUrl":"10.1007/s00401-024-02832-9","url":null,"abstract":"<div><p>Sudden unexplained death in childhood (SUDC) is death of a child ≥ 12 months old that is unexplained after autopsy and detailed analyses. Among SUDC cases, ~ 30% have febrile seizure (FS) history, versus 2–5% in the general population. SUDC cases share features with sudden unexpected death in epilepsy (SUDEP) and sudden infant death syndrome (SIDS), in which brainstem autonomic dysfunction is implicated. To understand whether brainstem protein changes are associated with FS history in SUDC, we performed label-free quantitative mass spectrometry on microdissected midbrain dorsal raphe, medullary raphe, and the ventrolateral medulla (n = 8 SUDC-noFS, n = 11 SUDC-FS). Differential expression analysis between SUDC-FS and SUDC-noFS at <i>p</i> &lt; 0.05 identified 178 altered proteins in dorsal raphe, 344 in medullary raphe, and 100 in the ventrolateral medulla. These proteins were most significantly associated with increased eukaryotic translation initiation (<i>p</i> = 3.09 × 10^–7, z = 1.00), eukaryotic translation elongation (<i>p</i> = 6.31 × 10^–49, z = 6.01), and coagulation system (<i>p</i> = 1.32 × 10^–5, z = 1.00). The medullary raphe had the strongest enrichment for altered signaling pathways, including with comparisons to three other brain regions previously analyzed (frontal cortex, hippocampal dentate gyrus, cornu ammonus). Immunofluorescent tissue analysis of serotonin receptors identified 2.1-fold increased 5HT2A in the medullary raphe of SUDC-FS (<i>p</i> = 0.025). Weighted gene correlation network analysis (WGCNA) of case history indicated that longer FS history duration significantly correlated with protein levels in the medullary raphe and ventrolateral medulla; the most significant gene ontology biological processes were decreased cellular respiration (<i>p</i> = 9.8 × 10^–5, corr = − 0.80) in medullary raphe and decreased synaptic vesicle cycle (<i>p</i> = 1.60 × 10^–7, corr = − 0.90) in the ventrolateral medulla. Overall, FS in SUDC was associated with more protein differences in the medullary raphe and was related with increased translation-related signaling pathways. Future studies should assess whether these changes result from FS or may in some way predispose to FS or SUDC.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02832-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated expression of the retrotransposon LINE-1 drives Alzheimer’s disease-associated microglial dysfunction","authors":"Nainika Roy,&nbsp;Imdadul Haq,&nbsp;Jason C. Ngo,&nbsp;David A. Bennett,&nbsp;Andrew F. Teich,&nbsp;Philip L. De Jager,&nbsp;Marta Olah,&nbsp;Falak Sher","doi":"10.1007/s00401-024-02835-6","DOIUrl":"10.1007/s00401-024-02835-6","url":null,"abstract":"<div><p>Aberrant activity of the retrotransposable element long interspersed nuclear element-1 (LINE-1) has been hypothesized to contribute to cellular dysfunction in age-related disorders, including late-onset Alzheimer’s disease (LOAD). However, whether LINE-1 is differentially expressed in cell types of the LOAD brain, and whether these changes contribute to disease pathology is largely unknown. Here, we examined patterns of LINE-1 expression across neurons, astrocytes, oligodendrocytes, and microglia in human postmortem prefrontal cortex tissue from LOAD patients and cognitively normal, age-matched controls. We report elevated immunoreactivity of the open reading frame 1 protein (ORF1p) encoded by LINE-1 in microglia from LOAD patients and find that this immunoreactivity correlates positively with disease-associated microglial morphology. In human iPSC-derived microglia (iMG), we found that CRISPR-mediated transcriptional activation of LINE-1 drives changes in microglial morphology and cytokine secretion and impairs the phagocytosis of amyloid beta (Aβ). We also find LINE-1 upregulation in iMG induces transcriptomic changes genes associated with antigen presentation and lipid metabolism as well as impacting the expression of many AD-relevant genes. Our data posit that heightened LINE-1 expression may trigger microglial dysregulation in LOAD and that these changes may contribute to disease pathogenesis, suggesting a central role for LINE-1 activity in human LOAD.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02835-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional profiling of murine glioma models highlights targetable immune evasion phenotypes","authors":"Nicholas Mikolajewicz,&nbsp;Nazanin Tatari,&nbsp;Jiarun Wei,&nbsp;Neil Savage,&nbsp;Adrian Granda Farias,&nbsp;Vassil Dimitrov,&nbsp;David Chen,&nbsp;Zsolt Zador,&nbsp;Kuheli Dasgupta,&nbsp;Magali Aguilera-Uribe,&nbsp;Yu-Xi Xiao,&nbsp;Seon Yong Lee,&nbsp;Patricia Mero,&nbsp;Dillon McKenna,&nbsp;Chitra Venugopal,&nbsp;Kevin R. Brown,&nbsp;Hong Han,&nbsp;Sheila Singh,&nbsp;Jason Moffat","doi":"10.1007/s00401-024-02831-w","DOIUrl":"10.1007/s00401-024-02831-w","url":null,"abstract":"<div><p>Cancer-intrinsic immune evasion mechanisms and pleiotropy are a barrier to cancer immunotherapy. This is apparent in certain highly fatal cancers, including high-grade gliomas and glioblastomas (GBM). In this study, we evaluated two murine syngeneic glioma models (GL261 and CT2A) as preclinical models for human GBM using functional genetic screens, single-cell transcriptomics and machine learning approaches. Through CRISPR genome-wide co-culture killing screens with various immune cells (cytotoxic T cells, natural killer cells, and macrophages), we identified three key cancer-intrinsic evasion mechanisms: NFκB signaling, autophagy/endosome machinery, and chromatin remodeling. Additional fitness screens identified dependencies in murine gliomas that partially recapitulated those seen in human GBM (e.g., UFMylation). Our single-cell analyses showed that different glioma models exhibited distinct immune infiltration patterns and recapitulated key immune gene programs observed in human GBM, including hypoxia, interferon, and TNF signaling. Moreover, in vivo orthotopic tumor engraftment was associated with phenotypic shifts and changes in proliferative capacity, with murine tumors recapitulating the intratumoral heterogeneity observed in human GBM, exhibiting propensities for developmental- and mesenchymal-like phenotypes. Notably, we observed common transcription factors and cofactors shared with human GBM, including developmental (<i>Nfia</i> and <i>Tcf4</i>), mesenchymal (<i>Prrx1</i> and <i>Wwtr1</i>), as well as cycling-associated genes (<i>Bub3</i>, <i>Cenpa</i>, <i>Bard1</i>, <i>Brca1</i>, and <i>Mis18bp1</i>). Perturbation of these genes led to reciprocal phenotypic shifts suggesting intrinsic feedback mechanisms that balance in vivo cellular states. Finally, we used a machine-learning approach to identify two distinct immune evasion gene programs, one of which represents a clinically-relevant phenotype and delineates a subpopulation of stem-like glioma cells that predict response to immune checkpoint inhibition in human patients. This comprehensive characterization helps bridge the gap between murine glioma models and human GBM, providing valuable insights for future therapeutic development.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02831-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid storage myopathy associated with sertraline treatment is an acquired mitochondrial disorder with respiratory chain deficiency","authors":"Carola Hedberg-Oldfors,&nbsp;Ulrika Lindgren,&nbsp;Kittichate Visuttijai,&nbsp;Yan Shen,&nbsp;Andreea Ilinca,&nbsp;Sara Nordström,&nbsp;Christopher Lindberg,&nbsp;Anders Oldfors","doi":"10.1007/s00401-024-02830-x","DOIUrl":"10.1007/s00401-024-02830-x","url":null,"abstract":"<div><p>Lipid storage myopathies are considered inborn errors of metabolism affecting the fatty acid metabolism and leading to accumulation of lipid droplets in the cytoplasm of muscle fibers. Specific diagnosis is based on investigation of organic aids in urine, acylcarnitines in blood and genetic testing. An acquired lipid storage myopathy in patients treated with the antidepressant drug sertraline, a serotonin reuptake inhibitor, has recently emerged as a new tentative differential diagnosis. We analyzed the muscle biopsy tissue in a group of 11 adult patients with muscle weakness and lipid storage myopathy which developed at a time when they were on sertraline treatment. This group comprise most patients with lipid storage myopathies in western Sweden during the recent nine-year period. By enzyme histochemistry, electron microscopy, quantitative proteomics, immunofluorescence of the respiratory chain subunits, western blot and genetic analyses we demonstrate that muscle tissue in this group of patients exhibit a characteristic morphological and proteomic profile. The patients also showed an acylcarnitine profile in blood suggestive of multiple acyl-coenzyme A dehydrogenase deficiency, but no genetic explanation was found by whole genome or exome sequencing. By proteomic analysis the muscle tissue revealed a profound loss of Complex I subunits from the respiratory chain and to some extent also deficiency of Complex II and IV. Most other components of the respiratory chain as well as the fatty acid oxidation and citric acid cycle were upregulated in accordance with the massive mitochondrial proliferation. The respiratory chain deficiency was verified by immunofluorescence analysis, western blot analysis and enzyme histochemistry. The typical ultrastructural changes of the mitochondria included pleomorphism, dark matrix and frequent round osmiophilic inclusions. Our results show that lipid storage myopathy associated with sertraline treatment is a mitochondrial disorder with respiratory chain deficiency and is an important differential diagnosis with characteristic features.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02830-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142712528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SMOC1 colocalizes with Alzheimer’s disease neuropathology and delays Aβ aggregation","authors":"Kaleah Balcomb,&nbsp;Caitlin Johnston,&nbsp;Tomas Kavanagh,&nbsp;Dominique Leitner,&nbsp;Julie Schneider,&nbsp;Glenda Halliday,&nbsp;Thomas Wisniewski,&nbsp;Margaret Sunde,&nbsp;Eleanor Drummond","doi":"10.1007/s00401-024-02819-6","DOIUrl":"10.1007/s00401-024-02819-6","url":null,"abstract":"<div><p>SMOC1 has emerged as one of the most significant and consistent new biomarkers of early Alzheimer’s disease (AD). Recent studies show that SMOC1 is one of the earliest changing proteins in AD, with levels in the cerebrospinal fluid increasing many years before symptom onset. Despite this clear association with disease, little is known about the role of SMOC1 in AD or its function in the brain. Therefore, the aim of this study was to examine the distribution of SMOC1 in human AD brain tissue and to determine if SMOC1 influenced amyloid beta (Aβ) aggregation. The distribution of SMOC1 in human brain tissue was assessed in 3 brain regions (temporal cortex, hippocampus, and frontal cortex) using immunohistochemistry in a cohort of 73 cases encompassing advanced AD, mild cognitive impairment (MCI), preclinical AD, and cognitively normal controls. The Aβ- and phosphorylated tau-interaction with SMOC1 was assessed in control, MCI, and advanced AD human brain tissue using co-immunoprecipitation, and the influence of SMOC1 on Aβ aggregation kinetics was assessed using Thioflavin-T assays and electron microscopy. SMOC1 strongly colocalized with a subpopulation of amyloid plaques in AD (43.8 ± 2.4%), MCI (32.8 ± 5.4%), and preclinical AD (28.3 ± 6.4%). SMOC1 levels in the brain strongly correlated with plaque load, irrespective of disease stage. SMOC1 also colocalized with a subpopulation of phosphorylated tau aggregates in AD (9.6 ± 2.6%). Co-immunoprecipitation studies showed that SMOC1 strongly interacted with Aβ in human MCI and AD brain tissue and with phosphorylated tau in human AD brain tissue. Thioflavin-T aggregation assays showed that SMOC1 significantly delayed Aβ aggregation in a dose-dependent manner, and electron microscopy confirmed that the Aβ fibrils generated in the presence of SMOC1 had an altered morphology. Overall, our results emphasize the importance of SMOC1 in the onset and progression of AD and suggest that SMOC1 may influence pathology development in AD.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02819-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BAG3’s dual roles in Parkinson’s disease and cardiomyopathy: benefit or liability?","authors":"Hui-Qi Qu,&nbsp;Hakon Hakonarson","doi":"10.1007/s00401-024-02837-4","DOIUrl":"10.1007/s00401-024-02837-4","url":null,"abstract":"","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142694804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathologic and clinical correlates of region-specific brain GFAP in Alzheimer’s disease","authors":"Jared M. Phillips,&nbsp;Rebecca L. Winfree,&nbsp;Mabel Seto,&nbsp;Julie A. Schneider,&nbsp;David A. Bennett,&nbsp;Logan C. Dumitrescu,&nbsp;Timothy J. Hohman","doi":"10.1007/s00401-024-02828-5","DOIUrl":"10.1007/s00401-024-02828-5","url":null,"abstract":"<div><p>Plasma glial fibrillary acidic protein (GFAP) is an emerging biomarker of Alzheimer’s disease (AD), with higher blood GFAP levels linked to faster cognitive decline, particularly among individuals with high brain amyloid burden. However, few studies have examined brain GFAP expression to clarify if peripheral associations reflect brain changes. This study aimed to correlate region-specific GFAP mRNA expression (<i>n</i> = 917) and protein abundance (n=386) with diverse neuropathological measures at autopsy in the Religious Orders Study and Rush Memory and Aging Project (ROS/MAP) and to characterize the interaction between brain GFAP and brain amyloid burden on downstream outcomes. We assessed GFAP gene expression in the dorsolateral prefrontal cortex, caudate nucleus, and posterior cingulate cortex with respect to core AD pathology (amyloid-β and tau), cerebrovascular (microinfarcts, macroinfarcts, and cerebral amyloid angiopathy [CAA]), proteinopathic (TDP-43, Lewy bodies), and cognitive outcomes. These associations were further examined at the protein level using tandem-mass tag proteomic measurements from the dorsolateral prefrontal cortex. We also assessed GFAP interactions with AD neuropathology on downstream outcomes. Cortical GFAP gene and protein expression were significantly upregulated in participants with a neuropathologically confirmed AD diagnosis at autopsy (all P_FDR &lt; 3.5e−4), but not in individuals positive for tau pathology and negative for amyloid pathology (all P_FDR &gt; 0.05). Higher cortical GFAP levels were associated with increased amyloid pathology, CAA pathology, and faster cognitive decline (all P_FDR &lt; 3.3e−3). GFAP’s associations with phosphorylated tau burden and cognition were influenced by amyloid burden, being most pronounced among amyloid-positive individuals, confirming previous in vivo biomarker observations. No associations were observed between <i>GFAP</i> gene expression and outcomes in the caudate nucleus. Our results support previous biomarker findings and suggest that higher brain GFAP levels are associated with higher brain amyloid burden and faster cognitive decline among amyloid-positive individuals.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02828-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuronal and oligodendroglial, but not astroglial, tau translates to in vivo tau PET signals in individuals with primary tauopathies","authors":"Luna Slemann,&nbsp;Johannes Gnörich,&nbsp;Selina Hummel,&nbsp;Laura M. Bartos,&nbsp;Carolin Klaus,&nbsp;Agnes Kling,&nbsp;Julia Kusche-Palenga,&nbsp;Sebastian T. Kunte,&nbsp;Lea H. Kunze,&nbsp;Amelie L. Englert,&nbsp;Yunlei Li,&nbsp;Letizia Vogler,&nbsp;Sabrina Katzdobler,&nbsp;Carla Palleis,&nbsp;Alexander Bernhardt,&nbsp;Alexander Jäck,&nbsp;Andreas Zwergal,&nbsp;Franziska Hopfner,&nbsp;Sebastian N. Roemer-Cassiano,&nbsp;Gloria Biechele,&nbsp;Sophia Stöcklein,&nbsp;Gerard Bischof,&nbsp;Thilo van Eimeren,&nbsp;Alexander Drzezga,&nbsp;Osama Sabri,&nbsp;Henryk Barthel,&nbsp;Gesine Respondek,&nbsp;Timo Grimmer,&nbsp;Johannes Levin,&nbsp;Jochen Herms,&nbsp;Lars Paeger,&nbsp;Marie Willroider,&nbsp;Leonie Beyer,&nbsp;Günter U. Höglinger,&nbsp;Sigrun Roeber,&nbsp;Nicolai Franzmeier,&nbsp;Matthias Brendel","doi":"10.1007/s00401-024-02834-7","DOIUrl":"10.1007/s00401-024-02834-7","url":null,"abstract":"<div><p>Tau PET has attracted increasing interest as an imaging biomarker for 4-repeat (4R)-tauopathy progressive supranuclear palsy (PSP). However, the translation of in vitro 4R-tau binding to in vivo tau PET signals is still unclear. Therefore, we performed a translational study using a broad spectrum of advanced methodologies to investigate the sources of [¹⁸F]PI-2620 tau PET signals in individuals with 4R-tauopathies, including a pilot PET autopsy study in patients. First, we conducted a longitudinal [¹⁸F]PI-2620 PET/MRI study in a 4-repeat-tau mouse model (PS19) and detected elevated [¹⁸F]PI-2620 PET signals in the presence of high levels of neuronal tau. An innovative approach involving cell sorting after radiotracer injection in vivo revealed higher tracer uptake in single neurons than in the astrocytes of PS19 mice. Regional [¹⁸F]PI-2620 tau PET signals during the lifetime correlated with the abundance of fibrillary tau and with autoradiography signal intensity in PSP patients and disease controls who underwent autopsy 2–63 months after tau PET. In autoradiography, tau-positive neurons and oligodendrocytes with a high AT8 density, but not tau-positive astrocytes, were the drivers of [¹⁸F]PI-2620 autoradiography signals in individuals with PSP. The high tau abundance in oligodendrocytes at the boundary of gray and white matter facilitated the identification of an optimized frontal lobe target region to detect the tau burden in patients with PSP. In summary, neuronal and oligodendroglial tau constitutes the dominant source of tau PET radiotracer binding in 4-repeat-tauopathies, translating to an in vivo signal.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02834-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide methylation profiling differentiates benign from aggressive and metastatic pituitary neuroendocrine tumors","authors":"Jelena Jotanovic,&nbsp;Henning Bünsow Boldt,&nbsp;Mark Burton,&nbsp;Marianne Skovsager Andersen,&nbsp;Daniel Bengtsson,&nbsp;Thomas Olsson Bontell,&nbsp;Bertil Ekman,&nbsp;Britt Edén Engström,&nbsp;Ulla Feldt-Rasmussen,&nbsp;Ansgar Heck,&nbsp;Antonia Jakovcevic,&nbsp;Jens Otto L. Jørgensen,&nbsp;Ivana Kraljevic,&nbsp;Jacek Kunicki,&nbsp;John R. Lindsay,&nbsp;Marco Losa,&nbsp;Paul Benjamin Loughrey,&nbsp;Dominique Maiter,&nbsp;Maria Maksymowicz,&nbsp;Emilija Manojlovic-Gacic,&nbsp;Jens Pahnke,&nbsp;Stephan Petersenn,&nbsp;Maria Petersson,&nbsp;Vera Popovic,&nbsp;Oskar Ragnarsson,&nbsp;Åse Krogh Rasmussen,&nbsp;Zita Reisz,&nbsp;Wolfgang Saeger,&nbsp;Camilla Schalin-Jäntti,&nbsp;David Scheie,&nbsp;Maria Rosa Terreni,&nbsp;Olli Tynninen,&nbsp;Ben Whitelaw,&nbsp;Pia Burman,&nbsp;Olivera Casar-Borota","doi":"10.1007/s00401-024-02836-5","DOIUrl":"10.1007/s00401-024-02836-5","url":null,"abstract":"<div><p>Aggressive pituitary neuroendocrine tumors (PitNETs)/adenomas are characterized by progressive growth despite surgery and all standard medical therapies and radiotherapy. A subset will metastasize to the brain and/or distant locations and are termed metastatic PitNETs (pituitary carcinomas). Studies of potential prognostic markers have been limited due to the rarity of these tumors. A few recurrent somatic mutations have been identified, and epigenetic alterations and chromosomal rearrangements have not been explored in larger cohorts of aggressive and metastatic PitNETs. In this study, we performed genome-wide methylation analysis, including copy-number variation (CNV) calculations, on tumor tissue specimens from a large international cohort of 64 patients with aggressive (48) and metastatic (16) pituitary tumors. Twelve patients with non-invasive pituitary tumors (Knosp 0–2) exhibiting an indolent course over a 5 year follow-up served as controls. In an unsupervised hierarchical cluster analysis, aggressive/metastatic PitNETs clustered separately from benign pituitary tumors, and, when only specimens from the first surgery were analyzed, three separate clusters were identified: aggressive, metastatic, and benign PitNETs. Numerous CNV events affecting chromosomal arms and whole chromosomes were frequent in aggressive and metastatic, whereas benign tumors had normal chromosomal copy numbers with only few alterations. Genome-wide methylation analysis revealed different CNV profiles and a clear separation between aggressive/metastatic and benign pituitary tumors, potentially providing biomarkers for identification of these tumors with a worse prognosis at the time of first surgery. The data may refine follow-up routines and contribute to the timely introduction of adjuvant therapy in patients harboring, or at risk of developing, aggressive or metastatic pituitary tumors.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"148 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00401-024-02836-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}