Acta Neuropathologica最新文献

{"title":"Seeding activity of human superoxide dismutase 1 aggregates in familial and sporadic amyotrophic lateral sclerosis postmortem neural tissues by real-time quaking-induced conversion","authors":"Justin K. Mielke,&nbsp;Mikael Klingeborn,&nbsp;Eric P. Schultz,&nbsp;Erin L. Markham,&nbsp;Emily D. Reese,&nbsp;Parvez Alam,&nbsp;Ian R. Mackenzie,&nbsp;Cindy V. Ly,&nbsp;Byron Caughey,&nbsp;Neil R. Cashman,&nbsp;Moses J. Leavens","doi":"10.1007/s00401-024-02752-8","DOIUrl":"10.1007/s00401-024-02752-8","url":null,"abstract":"<div><p>Amyotrophic lateral sclerosis (ALS) is a rapidly progressive neurodegenerative disease with average lifespan of 2–5 years after diagnosis. The identification of novel prognostic and pharmacodynamic biomarkers are needed to facilitate therapeutic development. Metalloprotein human superoxide dismutase 1 (SOD1) is known to accumulate and form aggregates in patient neural tissue with familial ALS linked to mutations in their <i>SOD1</i> gene. Aggregates of SOD1 have also been detected in other forms of ALS, including the sporadic form and the most common familial form linked to abnormal hexanucleotide repeat expansions in the Chromosome 9 open reading frame 72 (<i>C9ORF72</i>) gene. Here, we report the development of a real-time quaking-induced conversion (RT-QuIC) seed amplification assay using a recombinant human SOD1 substrate to measure SOD1 seeding activity in postmortem spinal cord and motor cortex tissue from persons with different ALS etiologies. Our SOD1 RT-QuIC assay detected SOD1 seeds in motor cortex and spinal cord dilutions down to 10^–5. Importantly, we detected SOD1 seeding activity in specimens from both sporadic and familial ALS cases, with the latter having mutations in either their <i>SOD1</i> or <i>C9ORF72</i> genes. Analyses of RT-QuIC parameters indicated similar lag phases in spinal cords of sporadic and familial ALS patients, but higher ThT fluorescence maxima by <i>SOD1</i> familial ALS specimens and sporadic ALS thoracic cord specimens. For a subset of sporadic ALS patients, motor cortex and spinal cords were examined, with seeding activity in both anatomical regions. Our results suggest SOD1 seeds are in ALS patient neural tissues not linked to <i>SOD1</i> mutation, suggesting that SOD1 seeding activity may be a promising biomarker, particularly in sporadic ALS cases for whom genetic testing is uninformative.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11182821/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Disruption of MAM integrity in mutant FUS oligodendroglial progenitors from hiPSCs","authors":"Yingli Zhu,&nbsp;Thibaut Burg,&nbsp;Katrien Neyrinck,&nbsp;Tim Vervliet,&nbsp;Fatemeharefeh Nami,&nbsp;Ellen Vervoort,&nbsp;Karan Ahuja,&nbsp;Maria Livia Sassano,&nbsp;Yoke Chin Chai,&nbsp;Arun Kumar Tharkeshwar,&nbsp;Jonathan De Smedt,&nbsp;Haibo Hu,&nbsp;Geert Bultynck,&nbsp;Patrizia Agostinis,&nbsp;Johannes V. Swinnen,&nbsp;Ludo Van Den Bosch,&nbsp;Rodrigo Furtado Madeiro da Costa,&nbsp;Catherine Verfaillie","doi":"10.1007/s00401-024-02748-4","DOIUrl":"10.1007/s00401-024-02748-4","url":null,"abstract":"","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11176091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141316451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early and selective localization of tau filaments to glutamatergic subcellular domains within the human anterodorsal thalamus","authors":"Barbara Sárkány,&nbsp;Csaba Dávid,&nbsp;Tibor Hortobágyi,&nbsp;Péter Gombás,&nbsp;Peter Somogyi,&nbsp;László Acsády,&nbsp;Tim J. Viney","doi":"10.1007/s00401-024-02749-3","DOIUrl":"10.1007/s00401-024-02749-3","url":null,"abstract":"<div><p>Widespread cortical accumulation of misfolded pathological tau proteins (ptau) in the form of paired helical filaments is a major hallmark of Alzheimer’s disease. Subcellular localization of ptau at various stages of disease progression is likely to be informative of the cellular mechanisms involving its spread. Here, we found that the density of ptau within several distinct rostral thalamic nuclei in post-mortem human tissue (<i>n</i> = 25 cases) increased with the disease stage, with the anterodorsal nucleus (ADn) consistently being the most affected. In the ADn, ptau-positive elements were present already in the pre-cortical (Braak 0) stage. Tau pathology preferentially affected the calretinin-expressing subpopulation of glutamatergic neurons in the ADn. At the subcellular level, we detected ptau immunoreactivity in ADn cell bodies, dendrites, and in a specialized type of presynaptic terminal that expresses vesicular glutamate transporter 2 (vGLUT2) and likely originates from the mammillary body. The ptau-containing terminals displayed signs of degeneration, including endosomal/lysosomal organelles. In contrast, corticothalamic axon terminals lacked ptau. The data demonstrate the involvement of a specific cell population in ADn at the onset of the disease. The presence of ptau in subcortical glutamatergic presynaptic terminals supports hypotheses about the transsynaptic spread of tau selectively affecting specialized axonal pathways.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11166832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations of CSF BACE1 with amyloid pathology, neurodegeneration, and cognition in Alzheimer’s disease","authors":"Feng Gao,&nbsp;Mengguo Zhang,&nbsp;Qiong Wang,&nbsp;Ming Ni,&nbsp;Chang Liu,&nbsp;Kexue Deng,&nbsp;Qiang Xie,&nbsp;Shicung Wang,&nbsp;Jiong Shi,&nbsp;Yong Shen,&nbsp;For CANDI Consortium","doi":"10.1007/s00401-024-02750-w","DOIUrl":"10.1007/s00401-024-02750-w","url":null,"abstract":"<div><p>Β-site amyloid precursor protein (APP) cleaving enzyme (BACE1) is a crucial protease in the production of amyloid-β (Aβ) in Alzheimer’s disease (AD) patients. However, the side effects observed in clinical trials of BACE1 inhibitors, including reduction in brain volume and cognitive worsening, suggest that the exact role of BACE1 in AD pathology is not fully understood. To further investigate this, we examined cerebrospinal fluid (CSF) levels of BACE1 and its cleaved product sAPPβ that reflects BACE1 activity in the China Aging and Neurodegenerative Disorder Initiative cohort. We found significant correlations between CSF BACE1 or sAPPβ levels and CSF Aβ40, Aβ42, and Aβ42/Aβ40 ratio, but not with amyloid deposition detected by 18F-Florbetapir PET. Additionally, CSF BACE1 and sAPPβ levels were positively associated with cortical thickness in multiple brain regions, and higher levels of sAPPβ were linked to increased cortical glucose metabolism in frontal and supramarginal areas. Interestingly, individuals with higher baseline levels of CSF BACE1 exhibited slower rates of brain volume reduction and cognitive worsening over time. This suggests that increased levels and activity of BACE1 may not be the determining factor for amyloid deposition, but instead, may be associated with increased neuronal activity and potentially providing protection against neurodegeneration in AD.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141295384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The necroptosis cell death pathway drives neurodegeneration in Alzheimer’s disease","authors":"Sriram Balusu,&nbsp;Bart De Strooper","doi":"10.1007/s00401-024-02747-5","DOIUrl":"10.1007/s00401-024-02747-5","url":null,"abstract":"<div><p>Although apoptosis, pyroptosis, and ferroptosis have been implicated in AD, none fully explains the extensive neuronal loss observed in AD brains. Recent evidence shows that necroptosis is abundant in AD, that necroptosis is closely linked to the appearance of Tau pathology, and that necroptosis markers accumulate in granulovacuolar neurodegeneration vesicles (GVD). We review here the neuron-specific activation of the granulovacuolar mediated neuronal-necroptosis pathway, the potential AD-relevant triggers upstream of this pathway, and the interaction of the necrosome with the endo-lysosomal pathway, possibly providing links to Tau pathology. In addition, we underscore the therapeutic potential of inhibiting necroptosis in neurodegenerative diseases such as AD, as this presents a novel avenue for drug development targeting neuronal loss to preserve cognitive abilities. Such an approach seems particularly relevant when combined with amyloid-lowering drugs.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11162975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141292997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinically unfavorable transcriptome subtypes of non-WNT/non-SHH medulloblastomas are associated with a predominance in proliferating and progenitor-like cell subpopulations","authors":"Konstantin Okonechnikov,&nbsp;Daniel Schrimpf,&nbsp;Jan Koster,&nbsp;Philipp Sievers,&nbsp;Till Milde,&nbsp;Felix Sahm,&nbsp;David T. W. Jones,&nbsp;Andreas von Deimling,&nbsp;Stefan M. Pfister,&nbsp;Marcel Kool,&nbsp;Andrey Korshunov","doi":"10.1007/s00401-024-02746-6","DOIUrl":"10.1007/s00401-024-02746-6","url":null,"abstract":"<div><p>The non-WNT/non-SHH (Grp3/Grp4) medulloblastomas (MBs) include eight second-generation subgroups (SGS; I–VIII) each with distinct molecular and clinical characteristics. Recently, we also identified two prognostically relevant transcriptome subtypes within each SGS MB, which are associated with unique gene expression signatures and signaling pathways. These prognostic subsets may be in connection to the intra-tumoral cell landscape that underlies SGS MB clinical-molecular diversity. Here, we performed a deconvolution analysis of the Grp3/Grp4 MB bulk RNA profiles using the previously identified single-cell RNA-seq reference dataset and focusing on variability in the cellular composition of SGS MB. RNA deconvolution analysis of the Grp3/Grp4 MB disclosed the subgroup-specific neoplastic cell subpopulations. Neuronally differentiated axodendritic GP3-C1 and glutamatergic GP4-C1 subpopulations were distributed within Grp3- and Grp4-associated SGS MB, respectively. Progenitor GP3-B2 subpopulation was prominent in aggressive SGS II MB, whereas photoreceptor/visual perception GP3/4-C2 cell content was typical for SGS III/IV MB. The current study also revealed significant variability in the proportions of cell subpopulations between clinically relevant SGS MB transcriptome subtypes, where unfavorable cohorts were enriched with cell cycle and progenitor-like cell subpopulations and, vice versa<i>,</i> favorable subtypes were composed of neuronally differentiated cell fractions predominantly. A higher than median proportion of proliferating and progenitor cell subpopulations conferred the shortest survival of the Grp3 and Grp 4 MB, and similar survival associations were identified for all SGS MB except SGS IV MB. In summary, the recently identified clinically relevant Grp3/Grp4 MB transcriptome subtypes are composed of different cell populations. Future studies should aim to validate the prognostic and therapeutic role of the identified Grp3/Grp4 MB inter-tumoral cellular heterogeneity. The application of the single-cell techniques on each SGS MB separately could help to clarify the clinical significance of subgroup-specific variability in tumor cell content and its relation with prognostic transcriptome signatures identified before.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141282612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration of gene expression and protein solubility of the PI 5-phosphatase SHIP2 are correlated with Alzheimer’s disease pathology progression","authors":"Kunie Ando,&nbsp;Fahri Küçükali,&nbsp;Emilie Doeraene,&nbsp;Siranjeevi Nagaraj,&nbsp;Eugenia Maria Antonelli,&nbsp;May Thazin Htut,&nbsp;Zehra Yilmaz,&nbsp;Andreea-Claudia Kosa,&nbsp;Lidia Lopez-Guitierrez,&nbsp;Carolina Quintanilla-Sánchez,&nbsp;Emmanuel Aydin,&nbsp;Ana Raquel Ramos,&nbsp;Salwa Mansour,&nbsp;Sabrina Turbant,&nbsp;Stéphane Schurmans,&nbsp;Kristel Sleegers,&nbsp;Christophe Erneux,&nbsp;Jean-Pierre Brion,&nbsp;Karelle Leroy","doi":"10.1007/s00401-024-02745-7","DOIUrl":"10.1007/s00401-024-02745-7","url":null,"abstract":"<div><p>A recent large genome-wide association study has identified <i>EGFR</i> (encoding the epidermal growth factor EGFR) as a new genetic risk factor for late-onset AD. SHIP2, encoded by <i>INPPL1</i>, is taking part in the signalling and interactome of several growth factor receptors, such as the EGFR. While <i>INPPL1</i> has been identified as one of the most significant genes whose RNA expression correlates with cognitive decline, the potential alteration of SHIP2 expression and localization during the progression of AD remains largely unknown. Here we report that gene expression of both <i>EGFR</i> and <i>INPPL1</i> was upregulated in AD brains. SHIP2 immunoreactivity was predominantly detected in plaque-associated astrocytes and dystrophic neurites and its increase was correlated with amyloid load in the brain of human AD and of 5xFAD transgenic mouse model of AD. While mRNA of <i>INPPL1</i> was increased in AD, SHIP2 protein undergoes a significant solubility change being depleted from the soluble fraction of AD brain homogenates and co-enriched with EGFR in the insoluble fraction. Using FRET-based flow cytometry biosensor assay for tau-tau interaction, overexpression of SHIP2 significantly increased the FRET signal while siRNA-mediated downexpression of SHIP2 significantly decreased FRET signal. Genetic association analyses suggest that some variants in <i>INPPL1</i> locus are associated with the level of CSF pTau. Our data support the hypothesis that SHIP2 is an intermediate key player of EGFR and AD pathology linking amyloid and tau pathologies in human AD.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11150309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141236295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternatively spliced ELAVL3 cryptic exon 4a causes ELAVL3 downregulation in ALS TDP-43 proteinopathy","authors":"Isabel Costantino,&nbsp;Alex Meng,&nbsp;John Ravits","doi":"10.1007/s00401-024-02732-y","DOIUrl":"10.1007/s00401-024-02732-y","url":null,"abstract":"","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11139733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuropathological findings in Down syndrome, Alzheimer’s disease and control patients with and without SARS-COV-2: preliminary findings","authors":"Ann-Charlotte E. Granholm,&nbsp;Elisabet Englund,&nbsp;Anah Gilmore,&nbsp;Elizabeth Head,&nbsp;William H. Yong,&nbsp;Sylvia E. Perez,&nbsp;Samuel J. Guzman,&nbsp;Eric D. Hamlett,&nbsp;Elliott J. Mufson","doi":"10.1007/s00401-024-02743-9","DOIUrl":"10.1007/s00401-024-02743-9","url":null,"abstract":"<div><p>The SARS-CoV-2 virus that led to COVID-19 is associated with significant and long-lasting neurologic symptoms in many patients, with an increased mortality risk for people with Alzheimer’s disease (AD) and/or Down syndrome (DS). However, few studies have evaluated the neuropathological and inflammatory sequelae in <i>postmortem</i> brain tissue obtained from AD and people with DS with severe SARS-CoV-2 infections. We examined tau, beta-amyloid (Aβ), inflammatory markers and SARS-CoV-2 nucleoprotein in DS, AD, and healthy non-demented controls with COVID-19 and compared with non-infected brain tissue from each disease group (total <i>n</i> = 24). A nested ANOVA was used to determine regional effects of the COVID-19 infection on arborization of astrocytes (Sholl analysis) and percent-stained area of Iba-1 and TMEM 119. SARS-CoV-2 antibodies labeled neurons and glial cells in the frontal cortex of all subjects with COVID-19, and in the hippocampus of two of the three DS COVID-19 cases. SARS-CoV-2-related alterations were observed in peri-vascular astrocytes and microglial cells in the gray matter of the frontal cortex, hippocampus, and para-hippocampal gyrus. Bright field microscopy revealed scattered intracellular and diffuse extracellular Aβ deposits in the hippocampus of controls with confirmed SARS-CoV-2 infections. Overall, the present preliminary findings suggest that SARS-CoV-2 infections induce abnormal inflammatory responses in Down syndrome.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11130011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of APOEε4 on the pTau interactome in sporadic Alzheimer’s disease","authors":"Manon Thierry,&nbsp;Jackeline Ponce,&nbsp;Mitchell Martà-Ariza,&nbsp;Manor Askenazi,&nbsp;Arline Faustin,&nbsp;Dominique Leitner,&nbsp;Geoffrey Pires,&nbsp;Evgeny Kanshin,&nbsp;Eleanor Drummond,&nbsp;Beatrix Ueberheide,&nbsp;Thomas Wisniewski","doi":"10.1007/s00401-024-02744-8","DOIUrl":"10.1007/s00401-024-02744-8","url":null,"abstract":"<div><p><i>APOE</i>^<i>ε4</i> is the major genetic risk factor for sporadic Alzheimer’s disease (AD). Although <i>APOE</i>^<i>ε4</i> is known to promote Aβ pathology, recent data also support an effect of <i>APOE</i> polymorphism on phosphorylated Tau (pTau) pathology. To elucidate these potential effects, the pTau interactome was analyzed across <i>APOE</i> genotypes in the frontal cortex of 10 advanced AD cases (<i>n</i> = 5 <i>APOE</i>^ε3/ε3 and <i>n</i> = 5 <i>APOE</i>^{<i>ε4/ε4</i>}), using a combination of anti-pTau pS396/pS404 (PHF1) immunoprecipitation (IP) and mass spectrometry (MS). This proteomic approach was complemented by an analysis of anti-pTau PHF1 and anti-Aβ 4G8 immunohistochemistry, performed in the frontal cortex of 21 advanced AD cases (<i>n</i> = 11 <i>APOE</i>^ε3/ε3 and <i>n</i> = 10 <i>APOE</i>^{<i>ε4/ε4</i>}). Our dataset includes 1130 and 1330 proteins enriched in IP_PHF1 samples from <i>APOE</i>^ε3/ε3 and <i>APOE</i>^{<i>ε4/ε4</i>} groups (fold change ≥ 1.50, IP_PHF1 <i>vs</i> IP_{IgG ctrl}). We identified 80 and 68 proteins as probable pTau interactors in <i>APOE</i>^ε3/ε3 and <i>APOE</i>^{<i>ε4/ε4</i>} groups, respectively (SAINT score ≥ 0.80; false discovery rate (FDR) ≤ 5%). A total of 47/80 proteins were identified as more likely to interact with pTau in <i>APOE</i>^{<i>ε3/ε3</i>}<i> vs APOE</i>^{<i>ε4/ε4</i>} cases. Functional enrichment analyses showed that they were significantly associated with the nucleoplasm compartment and involved in RNA processing. In contrast, 35/68 proteins were identified as more likely to interact with pTau in <i>APOE</i>^{<i>ε4/ε4</i>}<i> vs APOE</i>^{<i>ε3/ε3</i>} cases. They were significantly associated with the synaptic compartment and involved in cellular transport. A characterization of Tau pathology in the frontal cortex showed a higher density of plaque-associated neuritic crowns, made of dystrophic axons and synapses, in <i>APOE</i>^<i>ε4</i> carriers. Cerebral amyloid angiopathy was more frequent and severe in <i>APOE</i>^{<i>ε4/ε4</i>} cases. Our study supports an influence of <i>APOE</i> genotype on pTau-subcellular location in AD. These results suggest a facilitation of pTau progression to Aβ-affected brain regions in <i>APOE</i>^<i>ε4</i> carriers, paving the way to the identification of new therapeutic targets.</p></div>","PeriodicalId":7012,"journal":{"name":"Acta Neuropathologica","volume":"147 1","pages":""},"PeriodicalIF":9.3,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11108952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141074591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}