ACS Applied Nano Materials最新文献

{"title":"Nanoscale Interfacial Band Bending for Enhanced Cold Field Emission in GCN−ZnO Hybrids: Experimental and First-Principles Validation","authors":"P. Kumar,&nbsp;Anjali Dhariwal,&nbsp;Utsav P. Prajapati,&nbsp;P. N. Gajjar,&nbsp;N. S. Das,&nbsp;D. Banerjee","doi":"10.1021/acsanm.6c02935","DOIUrl":"https://doi.org/10.1021/acsanm.6c02935","url":null,"abstract":"<p>In this work, we explore the enhancement of cold field emission (CFE) characteristics in the hybrid zinc oxide (ZnO) and graphitic carbon nitride (GCN) hybrid structure, aiming to optimize their performance in vacuum microelectronics applications. ZnO, GCN, and GCN-ZnO nanostructures were synthesized via wet chemical synthesis and hydrothermal process and were characterized by using different techniques. The field emission scanning electron microscope and transmission electron microscopy analyses revealed the morphology and formation of interconnected ZnO nanorods anchored over sheet-like GCN structures. X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopic studies analyzed the chemical bond and elemental composition in the sample. To further understand the electronic behavior of the individual materials, density functional theory calculations based on the GGA-PBE approximation were performed to analyze the band structure and density of states. Furthermore, ANSYS Maxwell simulation confirmed an enhanced local electric field distribution and electron accumulation at the hetero-interface favorable for cold emission properties. The hybrid sample exhibited significantly improved CFE performance with a low turn-on field of 4.1 V/μm and a high emission current density of 138.8 μA/cm² compared to the individual materials. The enhanced emission characteristics are mainly attributed to the increased number of active emission sites, efficient interfacial charge transfer, and reduced potential barrier caused by band bending at the GCN-ZnO interface.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19232–19243"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese-Doped Fe3S4/Mo3S4 Nanoparticles on Nickel Foam as Durable Electrocatalysts for Water Electrolysis","authors":"Yuying Yang,&nbsp;Wenhui Zhang,&nbsp;Qiannan Sun,&nbsp;Daying Xu,&nbsp;Yihan Li,&nbsp;Guonv Cao,&nbsp;Yutian Liu,&nbsp;Min Zhou","doi":"10.1021/acsanm.6c03847","DOIUrl":"https://doi.org/10.1021/acsanm.6c03847","url":null,"abstract":"<p>Developing highly efficient, durable, and low-cost non-precious-metal bifunctional electrocatalysts is essential for sustainable water electrolysis. Herein, Mn-incorporated Fe₃S₄/Mo₃S₄ heterostructured nanoparticles were constructed in situ on nickel foam (NF) through a three-step hydrothermal sulfurization strategy. The resulting catalyst features a hierarchical nanoscale architecture consisting of interconnected nanosheets and nanoparticles, with intimate Fe₃S₄/Mo₃S₄ nanoscale interfaces and a homogeneous distribution of Mn. X-ray photoelectron spectroscopy (XPS) analysis reveals that Mn incorporation markedly modulates the local electronic environments of Fe, Mo, and S, promoting electronic redistribution within the Fe₃S₄/Mo₃S₄ heterostructure. In situ Raman spectroscopy further reveals potential-dependent structural evolution of the catalyst under operating conditions. During hydrogen evolution reaction (HER), the Mo−S vibrational signals progressively weaken with increasing cathodic polarization, indicating pronounced changes in the local Mo−S coordination environment. During oxygen evolution reaction (OER), the Fe−S-related signals remain comparatively stable, while the Mo−S-related region exhibits distinct potential-dependent evolution, demonstrating a dynamically responsive surface structure under anodic polarization. In 1.0 M KOH, the optimized Mn−Fe₃S₄/Mo₃S₄/NF-0.2 electrode requires overpotentials of only 29 and 212 mV to deliver 10 mA cm⁻² for HER and OER, respectively, with corresponding Tafel slopes of 75.99 and 24.93 mV dec⁻¹. When employed as both the cathode and anode, the resulting electrolyzer requires only 1.53 V to achieve 10 mA cm⁻² and maintains stable operation for 200 h. These results highlight the integration of hierarchical nanoscale architecture, nanoscale heterointerfaces, Mn-induced electronic modulation, and potential-dependent structural evolution for developing durable bifunctional electrocatalysts for alkaline water electrolysis.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19468–19478"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Interfacial Confinement and CVD-Derived Graphene Encapsulation in Silicon/Reduced Graphene Oxide/Graphene Composite for High-Performance Si Anodes","authors":"He Wang,&nbsp;Chen Zhang,&nbsp;Henglong Ren,&nbsp;Changbo Lu,&nbsp;Qiankun Xiang,&nbsp;Xing Zhao,&nbsp;Chun Lu,&nbsp;Bo Wang,&nbsp;Yongyi Song,&nbsp;Xinlong Ma","doi":"10.1021/acsanm.6c03269","DOIUrl":"https://doi.org/10.1021/acsanm.6c03269","url":null,"abstract":"<p>Silicon (Si)-based anodes are promising for next-generation lithium-ion batteries but suffer from severe volume expansion, structural degradation, and unstable interphase during cycling. Herein, a synergistic oxidation and controllable assembly strategy are proposed. First, Si nanoparticles (SiNPs) are co-oxidized with graphite, during which the SiO_<em>x</em> layer formed on the SiNP surface chemically bonds with the oxygen-containing functional groups of graphene oxide (GO) nanosheets. This interaction integrates SiNPs into the three-dimensional (3D) GO network via intercalation, wrapping, and anchoring, yielding a hierarchically encapsulated Si/GO precursor. Subsequently, chemical vapor deposition (CVD) is conducted to grow an outer graphene shell in situ on the Si/GO precursor, ultimately producing the Si/rGO/Gr composite. In this architecture, the 3D GO network provides multilevel buffering spaces, while the outer graphene layer serves as a conductive and mechanical confinement sheath, synergistically mitigating Si expansion and facilitating charge transfer. The optimized Si/rGO/Gr anode delivers reversible capacities of 1104.6 and 678.2 mAh g⁻¹ at 0.05 and 1 A g⁻¹, respectively. When assembled into a full cell with a LiFePO₄ cathode, the Si/rGO/Gr anode exhibits reversible capacities of 149.0 and 113.2 mAh g⁻¹ at 0.5 and 5 C, respectively. This work demonstrates that the combination of synergistic oxidation, controllable assembly, and CVD graphene encapsulation offers a viable synthetic route toward high-stability Si-based anodes.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19293–19306"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CuGaS2 Quantum Dots on the Hollow Spheres of TiO2 as S-Scheme Heterostructures for Efficient Photocatalytic Degradation of Methyl Orange","authors":"Minghao Zhou,&nbsp;Yanlai Wang","doi":"10.1021/acsanm.6c03624","DOIUrl":"https://doi.org/10.1021/acsanm.6c03624","url":null,"abstract":"<p>Photocatalysis is a promising method for treating organic pollutants. However, the wide band gap of TiO₂ limits its photocatalytic performance. In this study, CuGaS₂ quantum dots (QDs) were grown in situ on TiO₂ hollow spheres to construct a heterojunction for methyl orange (MO) removal. The composite with 10 wt % CuGaS₂ showed the best performance. It removed 95.72% of MO within 30 min under UV irradiation (<em>k</em> = 0.1031 min⁻¹), which was 1.6 and 11.1 times higher than those of TiO₂ and CuGaS₂ QDs, respectively. The catalytic performance remains stable over repeated cycling. In addition, radical scavenging experiments and band structure analysis confirm an S-scheme mechanism.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19373–19380"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Focused Electron Beam-Induced Deposition of Oblique Co and Fe Nanowires: Compositional and Magnetic Characterisation for Functional 3D Nanostructures","authors":"Aurys Šilinga,&nbsp;Keir L. Edgar,&nbsp;András Kovács,&nbsp;Stephen McVitie,&nbsp;Rafal E. Dunin-Borkowski,&nbsp;Kayla Fallon,&nbsp;Trevor P. Almeida","doi":"10.1021/acsanm.6c02497","DOIUrl":"https://doi.org/10.1021/acsanm.6c02497","url":null,"abstract":"<p>Focused electron beam induced deposition (FEBID) is an additive manufacturing technique uniquely suited for fabricating functional 3D nanostructures for a range of applications, including spintronic devices. However, the variation of growth dynamics associated with lateral movement of the electron beam results in structures with non-uniform composition when fabricating intricate 3D geometries. Herein, we measure changes in atomic composition and corresponding changes in magnetic induction in 3D ferromagnetic nanostructures with overhanging elements, e.g. bridges or arches. To investigate the effects of electron beam lateral movement, we fabricated Co and Fe nanowire (NW) structures with growth angle relative to the optic axis varying from 0° to 90°. The (scanning) transmission electron microscopy techniques of electron energy loss spectroscopy and off-axis electron holography were performed to map the NW elemental composition and magnetic induction as a function of NW growth angle. Comparison of the results reveals a reduction in metal content with increased growth angle in FEBID NWs. The magnitude of metal content reduction can be tuned by controlling electron beam parameters, and ferromagnetic NWs with approximately equal metal content at growth angles from 0° to 60° were fabricated by using the lowest viable electron beam voltage and the highest viable beam current to reduce the interaction volume and increase the metal content, respectively.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19185–19197"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Index CuI: A Promising Material Platform for Nanoscale Integrated Photonics Waveguides","authors":"Zhuoran Ji,&nbsp;Yuzhao Ouyang,&nbsp;Keqi Xia,&nbsp;Jialin Yang,&nbsp;Tian Shang,&nbsp;Hui Peng,&nbsp;Xiaodong Tang,&nbsp;Chang Yang","doi":"10.1021/acsanm.6c03018","DOIUrl":"https://doi.org/10.1021/acsanm.6c03018","url":null,"abstract":"<p>The rapid development of nanoscale integrated photonics demands core materials combining high transparency, high refractive index, and low optical loss. In this work, semi-transparent polycrystalline copper iodide (CuI) bulks were fabricated via the vertical Bridgman technique. Structural characterization reveals dense, large-grained microstructures. To reliably evaluate the material’s intrinsic optical parameters, spectroscopic ellipsometry was performed on a nanometer-thick companion CuI thin film, yielding a high refractive index (∼2.2) that ensures strong optical confinement when paired with common low-index claddings and strong subwavelength optical confinement. Critically, to assess the intrinsic optical transport properties before integrating into nanoscale optical waveguides, and exclude the influence of micro−nano fabrication complexities, a polycrystalline bulk waveguide configuration was utilized. Remarkably, despite the presence of grain boundaries, the material exhibits an attenuation as low as 20 dB/cm in the 900−1100 nm spectral range. This performance, achieved in a non-optimized polycrystalline state, decisively demonstrates the excellent intrinsic waveguiding potential of CuI. These findings establish CuI as a highly promising candidate for next-generation high-index-contrast photonic devices and provide a solid foundation for future CuI-based integrated optics.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19261–19269"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149173413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PVA/Sodium Alginate/PEDOT:PSS-Based Multifunctional Hydrogels with Rapid Self-Healing, High Stretchability, and Touch-Sensing Capability","authors":"Mohammad Zafar Abbas,&nbsp;Karamat Subhani,&nbsp;Premika Govindaraj,&nbsp;Setareh Elyasi,&nbsp;Vimukthi Dananjaya,&nbsp;Harald Oehler,&nbsp;Robert Brüll,&nbsp;Nisa V. Salim","doi":"10.1021/acsanm.6c02560","DOIUrl":"https://doi.org/10.1021/acsanm.6c02560","url":null,"abstract":"<p>The development of stretchable, self-healing, conducting hydrogels is crucial in wearable electronic devices, health monitoring, strain sensing, biosensing, etc. In this study, we developed a highly stretchable, rapid self-healing, adhesive, conductive hydrogel composite containing polyvinyl alcohol (PVA), natural polymer sodium alginate (SA), poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) conductive polymer, and crosslinking agent boric acid (BA), which induces rapid gelation at the nanoscale. PVA (8 wt %)/Alg (2 wt %)/PEDOT:PSS (0.1 wt %) hydrogels show high mechanical strength and stretchability with more than 30-fold elongation (3000% without breaking) when stretched manually. The studied hydrogel shows a rapid self-healing response, which is attributed to reversible and dynamic crosslinking boron-ester (B−O−C) bonds between boric acid and PVA. The hydrogel exhibits a very high constant (e′) of the order of 10⁹, originating from interfacial polarization arising from a highly crosslinked porous polymer nano-network. The electrical conductivity of the hydrogel is evidenced by the LED lights turning after connection. Changes in LED brightness during stretching, twisting, and release demonstrate the strain sensitivity of the <strong>PVA/Alg/PEDOT:PSS</strong> hydrogel. The hydrogel’s electrical resistance responded reversibly to applied weights, showing cyclic resistance/conductivity changes of 20%, 55%, 65%, and 80% under 50, 100, 200, and 500 g loads, respectively. These results confirm the material’s piezoresistive behavior and its potential as a pressure sensor for flexible electronic devices. By integrating electrical impedance tomography (EIT), the hydrogel was also able to distinguish between light touch and press stimuli, enabling detection of both pressure intensity and spatial distribution for applications in soft robotics and wearable electronics.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19198–19210"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149173417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic-Enabled Conductive Alginate Hydrogel with CeO2 Nanozymes and Electrical Stimulation for Synergistic Diabetic Wound Healing","authors":"Zhilin Xie,&nbsp;A. M. Omer,&nbsp;Xiao-kun Ouyang,&nbsp;Nan Wang","doi":"10.1021/acsanm.6c03666","DOIUrl":"https://doi.org/10.1021/acsanm.6c03666","url":null,"abstract":"<p>Diabetic wounds feature sustained hyperglycemia, intense oxidative stress, frequent bacterial invasion and compromised angiogenic activity. These factors jointly form a pathological microenvironment that hinders routine therapeutic interventions. Current dressings typically address only one or two of these barriers, leaving the complex interplay of redox imbalance, infection, and bioelectrical dysfunction insufficiently tackled. Herein, we report a probiotic-enabled conductive alginate double-network hydrogel embedded with CeO₂ nanozymes (SAMCL) that integrates redox regulation, microecological intervention, and bioelectrical support within a single platform. The CeO₂ efficiently scavenge reactive oxygen species and generate oxygen, while metabolically active <em>Limosilactobacillus</em><em>reuteri</em> produces reuterin, endowing the hydrogel with potent broad-spectrum antibacterial and antibiofilm activities. The hydrogel retains recoverable ionic conductivity, enabling electrical stimulation to further promote macrophage M2 polarization and angiogenesis. In a diabetic mouse infected wound model, the SAMCL hydrogel with electrical stimulation achieved near-complete wound closure on day 14, with regenerated cutaneous follicles and glandular tissues, reduced inflammation, and normalized hypoxic signaling. This study develops a feasible approach to modulate the microenvironment of diabetic wounds, and lays a foundation for the clinical translation of multifunctional chronic wound dressings.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19381–19399"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149173610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-Assembling Betulinic Acid and Squalene Nanoparticles Enhance the Blood−Brain Barrier Reinforcing Activity of Trehalose","authors":"Veronica Fontanini,&nbsp;Arianna Amenta,&nbsp;Alice Maiocchi,&nbsp;Barbara Vergani,&nbsp;Biagio Eugenio Leone,&nbsp;Davide Camazzola,&nbsp;Fabrizia Claudia Guarnieri,&nbsp;Luca Murru,&nbsp;Luciana Marinelli,&nbsp;Valeria La Pietra,&nbsp;Francesca Urbano,&nbsp;Mario Mauri,&nbsp;Giuseppe Paglia,&nbsp;Pierfausto Seneci,&nbsp;Francesca Re","doi":"10.1021/acsanm.6c03130","DOIUrl":"https://doi.org/10.1021/acsanm.6c03130","url":null,"abstract":"<p>Trehalose, a natural disaccharide with well-established neuroprotective effects in the central nervous system (CNS), has been extensively investigated for its anti-inflammatory, protein-stabilizing, and autophagy-inducing activities. Despite these beneficial CNS effects, its potential impact on the blood−brain barrier (BBB), a key regulator of brain homeostasis and neurological health, remains poorly understood. Here, we demonstrate that trehalose reinforces the in vitro BBB by increasing transendothelial electrical resistance and reducing dextran permeability in hCMEC/D3 monolayers. These functional improvements were associated with AMPK activation and upregulation of the tight junction protein ZO-1, where findings were corroborated in vivo by increased ZO-1 expression in trehalose-treated C57BL/6J mice. Molecular docking further suggested the preferential binding of trehalose to the ZO-1 PDZ3-SH3 domain, supporting a potential mechanism for tight junction stabilization. These findings establish trehalose as a promising BBB-protective agent through complementary molecular mechanisms. Nevertheless, the therapeutic exploitation of free trehalose is hindered by its unfavorable pharmacokinetic properties. To address these limitations and improve its delivery, we engineered four self-assembling trehalose nanoparticles (Treh-NAs) based on mono- or bis-betulinic acid and mono- or bis-squalene conjugates. Nanoparticle encapsulation preserved the barrier-enhancing activity of trehalose while markedly reducing the effective dose in vitro. Among the formulations, the mono-betulinic acid nanoparticles displayed the highest endothelial permeability and the strongest barrier-reinforcing effect. Collectively, these results provide a rationale for the development of trehalose-based nanomedicines aimed at reinforcing the BBB and improving therapeutic interventions for CNS diseases associated with BBB dysfunction, in which the neuroprotective properties of trehalose may provide additional therapeutic benefit.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19270–19284"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149188001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Steric Stabilization of Lipid Nanoparticles by Sequence-Defined Polymer−Lipids Enhances Gene Expression","authors":"Evrim Ümit Kuzucu,&nbsp;Alireza Kavand,&nbsp;Arséne Timothée Tergalinsky,&nbsp;Lorenz Herbster,&nbsp;David Wang,&nbsp;Alessandra Cavegn,&nbsp;Perrine Robin,&nbsp;Jörg Huwyler,&nbsp;Pascal Detampel,&nbsp;Sandrine Gerber-Lemaire","doi":"10.1021/acsanm.6c04234","DOIUrl":"https://doi.org/10.1021/acsanm.6c04234","url":null,"abstract":"<p>Polyethylene glycol (PEG) conjugated lipids are frequently used for the design of lipid nanoparticles (LNPs) in the field of gene therapy. However, conventional PEG−lipids contain a distribution of polymer chain lengths. To overcome this limitation, we have prepared a structurally defined PEG-alternative polymer-lipid platform synthesized via solid-phase peptide synthesis (SPPS), enabling precise control over polymer chain length and lipid anchor identity. A library of chain-length-defined poly(succinamide−4,7,10-trioxatridecane)_<em>n</em>−lipid (poly(SA-TTD)_<em>n</em>-lipid) conjugates, with hydrophilic polymer segments ranging from 923 to 2133 g/mol and varied lipid tails, was synthesized and formulated into DNA-loaded LNPs using microfluidic mixing. Systematic variation of polymer length and lipid tail structure revealed their distinct influence on NP size, colloidal stability, and DNA encapsulation efficiency. All formulations produced particles with narrow size distributions (PDI &lt;0.2) with efficient DNA association. In vitro studies demonstrated that selected PEG-alternative LNPs significantly enhanced transfection efficiency compared to PEG2000-containing controls, achieving up to 18-fold higher transgene expression. Notably, these formulations also exhibited an approximately fourfold higher endosomal escape-associated signal. In vivo evaluation in zebrafish larvae (ZFL) further confirmed efficient systemic circulation and superior transgene expression without apparent toxicity. In summary, this work establishes a modular, sequence-defined polymer-lipid platform that provides a promising PEG alternative for LNP-mediated gene delivery.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"9 41","pages":"19525–19536"},"PeriodicalIF":5.8,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149187984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}