ACS Applied Nano Materials最新文献

{"title":"Hydrogen Storage Capacities in Nanoporous M2(m-dobdc) Metal–Organic Frameworks at Near Ambient Temperatures","authors":"Himani Joshi,&nbsp; and ,&nbsp;Srimanta Pakhira*,&nbsp;","doi":"10.1021/acsanm.5c02933","DOIUrl":"https://doi.org/10.1021/acsanm.5c02933","url":null,"abstract":"<p >Green hydrogen presents a cleaner alternative to petroleum-based energy, with onboard storage challenges addressed by metal–organic frameworks (MOFs). This study evaluates M₂(<i>m</i>-dobdc) MOFs (M = Mn, Fe, Co, and Ni) for H₂-storage using Grand Canonical Monte Carlo simulations (GCMC) at five distinct temperatures (77, 160, 198, 233, and 298 K) and pressures of 1–100 bar. The Fe₂(<i>m</i>-dobdc) MOF achieves a promising volumetric H₂ storage capacity of 51.2 g/L under cryogenic conditions, meeting the target of the Department of Energy, United States of America (USDOE), while the calculated value of the gravimetric uptake is 4.2 wt %, which approaches the USDOE 2025 target of 5.5 wt %. M₂(<i>m</i>-dobdc) MOF series reflect exceptional volumetric uptake capacities at room temperature, ranging from 11.0 to 12.3 g/L, while gravimetric capacities are moderate, indicating key challenges in physisorption-based nanoporous materials when operating at ambient conditions. Density functional theory (DFT) calculations reveal the heat of H₂ adsorption (<i>Q</i>_st) ranging from −15 to −18 kJ/mol in this M₂(<i>m</i>-dobdc) MOF series, confirming the reversible physisorption phenomenon. Our results highlight the potential of M₂(<i>m</i>-dobdc) MOFs for mobile H₂-storage applications, with deliverable volumetric capacities ranging from 36.3 to 40.8 g/L and gravimetric uptake ranging from 2.7 to 3.4 wt % under a temperature–pressure swing range (77 K/100 bar to 160 K/5 bar). The present investigation indicates that the M₂(<i>m</i>-dobdc) MOFs have shown advances of the USDOE target and promise to meet the practical onboard hydrogen-storage requirements in automobile applications. Their balanced gravimetric-volumetric capacities make them promising candidates for near-future implementation in green energy systems.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19167–19178"},"PeriodicalIF":5.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SANS/SAXS Study to Unravel the Protein Corona Dynamics Using Deuterated Human Serum Albumin Interactions with Plasmonic Au Nanostructures","authors":"Anastasiia Tukova*,&nbsp;, ,&nbsp;Andrew E. Whitten,&nbsp;, ,&nbsp;Anthony P. Duff,&nbsp;, ,&nbsp;Valérie Laux,&nbsp;, ,&nbsp;John W. White,&nbsp;, ,&nbsp;Alison Rodger,&nbsp;, ,&nbsp;Yuling Wang,&nbsp;, and ,&nbsp;Alfonso E. Garcia-Bennett,&nbsp;","doi":"10.1021/acsanm.5c02983","DOIUrl":"https://doi.org/10.1021/acsanm.5c02983","url":null,"abstract":"<p >Protein corona (PC) significantly influences the stability and biological interactions of nanoparticles and has a dynamic behavior, particularly with respect to the exchange between the “soft” (loosely bound) and “hard” (tightly bound) PC layers. This study employs small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) to investigate the structural and dynamic aspects of interactions of human serum albumin (HSA) with gold nanoparticles and gold nanostars. We utilized recombinant deuterated human serum albumin (dHSA) to enhance neutron contrast, enabling <i>in situ</i> tracking of protein binding and exchange without altering the protein structure or function. As a control, recombinant nondeuterated HSA (nHSA) was used to assess dynamic protein exchange. Structural characterization confirmed similar interactions of dHSA and nHSA with gold surfaces, ensuring reliability in PC dynamics studies. SAXS characterization revealed minimal protein binding to gold nanostars (AuNSs), suggesting a reduced propensity for PC formation compared with spherical gold nanoparticles (AuNPs). SANS measurements, conducted under contrast matching conditions in deuterated water, allowed precise differentiation between free and nanoparticle-bound proteins, demonstrating that PC molecule exchange occurred homogeneously within 4 h of scattering measurements and remains stable for at least 12 h. These findings provide insights into molecule behavior within PC, with implications for nanoparticle design in biomedical applications.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19179–19191"},"PeriodicalIF":5.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size of Pt Nanoparticles Determines the Effect of Steam Treatment on Their Performance for Catalytic CO Oxidation","authors":"Liyuan Chen,&nbsp;, ,&nbsp;Yumeng Xu,&nbsp;, ,&nbsp;Yong Zheng,&nbsp;, ,&nbsp;Fei Huang*,&nbsp;, and ,&nbsp;Ying Zheng,&nbsp;","doi":"10.1021/acsanm.5c03400","DOIUrl":"https://doi.org/10.1021/acsanm.5c03400","url":null,"abstract":"<p >CO oxidation catalyzed by Pt group metal nanoparticle catalysts is an important reaction in many fields, such as the operation of fuel cells and automotive exhaust gas alleviation. However, improving resistance to poisoning by H₂O or CO₂, which are present in feed gas or exhaust gas, is still a great challenge for Pt catalysts. Herein, by employing hexaaluminate (BaFeAl₁₁O₁₉, denoted as H) as a support, a correlation between the size of Pt nanoparticles and the effect of steam treatment (7 vol % H₂O, 500 °C 1 h) was found. The aged Pt nanoparticle catalysts with a size above 1.0 nm (1Pt/H-A) exhibited enhanced catalytic activity, cycling stability, and resistance to H₂O and CO₂ poisoning in CO oxidation compared with the fresh counterpart (1Pt/H). By contrast, there was a negligible change in physicochemical properties and catalytic performance over Pt nanocluster catalysts (&lt;1.0 nm) before and after the treatment (0.5Pt/H vs 0.5Pt/H-A). Such a promoting effect can be attributed to enhanced metal–support interaction after the steam treatment, which induced an electronic effect on Pt nanoparticles and improved oxygen reactivity for CO oxidation.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19422–19432"},"PeriodicalIF":5.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gd3+-Doped Biocompatible Fluorinated Carbon Dots for Bimodal Bioimaging Applications","authors":"Anna Topchylo,&nbsp;, ,&nbsp;Konstantin Paliienko,&nbsp;, ,&nbsp;Alexander Zaderko,&nbsp;, ,&nbsp;Tetyana Nychyporuk,&nbsp;, ,&nbsp;Alain Géloën,&nbsp;, ,&nbsp;Olha Pylypova,&nbsp;, ,&nbsp;Ivan Ivanov,&nbsp;, ,&nbsp;Valeriy Skryshevsky*,&nbsp;, and ,&nbsp;Vladimir Lysenko,&nbsp;","doi":"10.1021/acsanm.5c03446","DOIUrl":"https://doi.org/10.1021/acsanm.5c03446","url":null,"abstract":"<p >Fluorinated carbon dots (FCDs) represent a promising class of nanomaterials for biomedical applications owing to their biocompatibility, high fluorescence, and chemical stability. In this work, we report for the first time the synthesis and systematic investigation of Gd³⁺-doped FCDs (Gd-FCDs) obtained via a solvothermal route using urea, anhydrous citric acid, 3-(trifluoromethyl)aniline, and gadolinium(III) chloride hexahydrate. By incorporating paramagnetic Gd³⁺ ions into fluorinated fluorescent carbon dots, we aimed to create multifunctional nanoprobes capable of simultaneous fluorescence and magnetic resonance imaging (MRI). Comprehensive characterization demonstrated that Gd³⁺ doping significantly altered the structural and optical properties of the FCDs. While pristine FCDs were ultrasmall (2–8 nm), Gd-FCDs exhibited larger sizes (40–80 nm) due to ion-induced aggregation. During the synthesis process, Gd³⁺ ions are efficient positively charged centers stimulating formation of FCDs around them, resulting in bigger final complexes. Zeta potentials of FCDs and Gd-FCDs are −27.8 mV and −1.5 mV, respectively. UV–vis and fluorescence analyses revealed changes in electronic transitions and reduced fluorescence intensity, consistent with the introduction of nonradiative pathways by Gd³⁺ ions. Time-resolved fluorescence studies further confirmed the modified exciton dynamics. Importantly, proton relaxation measurements showed drastic reductions in both <i>T</i>₁ and <i>T</i>₂ relaxation times for Gd-FCDs in the concentration range 0.015–4 g/L, highlighting their strong MRI contrast capability across different magnetic field strengths. Cell toxicity measurements on 3T3-L1 biological cells show that all the FCDs revealed no toxicity against cells, indicating their complete biological compatibility at the concentration levels between 0.175 and 0.334 mg/mL. Its efficient penetration into cell nuclei enables robust fluorescent cell labeling across the visible spectrum.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19453–19463"},"PeriodicalIF":5.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trace Level Acetone Detection via a Schottky-Contacted SrFeO3–Ti3C2Tx Nanocomposite Sensor","authors":"Adyasha Das,&nbsp;, ,&nbsp;Tanushri Das,&nbsp;, ,&nbsp;Rakesh Parida,&nbsp;, ,&nbsp;Adarsh Kumar,&nbsp;, ,&nbsp;Jin Yong Lee,&nbsp;, ,&nbsp;Mrinal Pal,&nbsp;, and ,&nbsp;Priyabrat Dash*,&nbsp;","doi":"10.1021/acsanm.5c03341","DOIUrl":"https://doi.org/10.1021/acsanm.5c03341","url":null,"abstract":"<p >Detection of acetone holds importance due to its adverse implications on the environment, industries, and human health. This work displays the successful formation of a SrFeO₃(SFO)–Ti₃C₂T_<i>x</i> (MX) Schottky-contacted composite sensor and its usage in detecting acetone effectively. The SFO–MX sensor displayed a higher response than the pristine SFO by displaying a high response of 99.5% toward 100 ppm of acetone at room temperature. The sensor could even sense a lower acetone concentration down to 250 ppb with a response of 20.5% at room temperature. The rapid response time (7 s) and recovery time (23 s) of the sensor make it highly worthy in real-time applications. The sensor demonstrated high selectivity, repeatability, and a good response even under humid conditions. The high response of the composite sensor is attributed to its high surface area, high adsorbed and vacant oxygen generation, defect site formations, and high interlayer spacing, which are confirmed by various studies. The stable reaction condition on the interaction of the SFO–MX with acetone was confirmed by the DFT analysis. This study gives future insight into the design of such a Schottky-contacted sensor for faster detection of acetone at room-temperature diabetes diagnosis through breath analysis.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19342–19361"},"PeriodicalIF":5.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuning Layered Double Hydroxides from Trimetallic to High-Entropy Nanomaterials for Enhanced Oxygen Evolution in Alkaline Environments","authors":"Chandrasekaran Pitchai,&nbsp;, ,&nbsp;Chao-Fang Huang,&nbsp;, and ,&nbsp;Chih-Ming Chen*,&nbsp;","doi":"10.1021/acsanm.5c03590","DOIUrl":"https://doi.org/10.1021/acsanm.5c03590","url":null,"abstract":"<p >Layered double hydroxides (LDHs) enriched with multiple metal elements and high-entropy alloys (HEAs) have gained attention as effective catalysts for the oxygen evolution reaction (OER). In this study, we report the hydrothermal synthesis of high-entropy FeCoCrMnCu (FCCMC) LDHs, aiming to develop cost-efficient and durable electrocatalysts. Extensive material characterization using FE-SEM, HR-TEM, XRD, EDX, and XPS confirmed the successful fabrication of FCCMC LDHs with uniform elemental dispersion and well-defined crystal structure. When tested under alkaline conditions, FCCMC LDHs displayed superior OER performance compared to their ternary (FeCoCr, FCC) and quaternary (FeCoCrMn, FCCM) analogs. Specifically, FCCMC achieved a notably low overpotential of 295 mV at 10 mA cm^–2 and a Tafel slope of 119.5 mV dec^–1, suggesting more favorable catalytic kinetics. Moreover, the FCCMC catalyst demonstrated impressive operational stability, maintaining activity over 74 h without significant performance loss. Collectively, these results identify FCCMC LDHs as a highly promising candidate for advancing efficient and stable electrocatalysts, supporting future progress in clean and sustainable energy technologies.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19558–19567"},"PeriodicalIF":5.5,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsanm.5c03590","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation Of Protein-Encapsulated Nanoparticles Using Polysuccinimide-Oleylamine For Sustained Protein Release","authors":"Xiangxun Chen,&nbsp;, ,&nbsp;Cuong Hung Luu,&nbsp;, ,&nbsp;Haotian Cha,&nbsp;, ,&nbsp;Shehzahdi S. Moonshi,&nbsp;, ,&nbsp;Jun Zhang,&nbsp;, ,&nbsp;Nam-Trung Nguyen,&nbsp;, and ,&nbsp;Hang Thu Ta*,&nbsp;","doi":"10.1021/acsanm.5c04092","DOIUrl":"https://doi.org/10.1021/acsanm.5c04092","url":null,"abstract":"<p >The clinical use of biologics for acute disease management is constrained by rapid clearance and systemic accumulation, which may compromise therapeutic outcomes. While sustained release nanoparticles are well-established for chronic conditions, analogous strategies for acute indications─particularly those requiring controlled release within 48 h─remain insufficiently developed. This study explores polysuccinimide-oleylamine (PSI-OA), a polymer with a tunable dissolution profile, for protein delivery. Using inverse flash nanoprecipitation and a herringbone T-shaped microfluidic mixer, PSI-OA nanoparticles encapsulating bovine serum albumin and lipase were fabricated. Optimized synthesis parameters yielded nanoparticles with less than 200 nm, with encapsulation efficiencies of roughly 75 and 98%, respectively, while retaining enzymatic activity. Both systems demonstrated ∼50% release at 24 h and full release by 48 h. These findings underscore the potential of PSI-OA nanoparticles for short-term sustained delivery of protein therapeutics in acute care settings.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19658–19667"},"PeriodicalIF":5.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Waterproof, Breathable, and Durable Electrospun Polyacrylonitrile/Isophorone Diisocyanate Nanofibrous Membranes Modified by a Fluorine-Free Hydrophobic Coating for Applications in Textiles","authors":"Haiyan Liao,&nbsp;, ,&nbsp;Mengyao Liu,&nbsp;, ,&nbsp;Wutong Ding,&nbsp;, ,&nbsp;Shibei Yao,&nbsp;, ,&nbsp;Chaoyu Zhu,&nbsp;, ,&nbsp;Huifang Zhang,&nbsp;, ,&nbsp;Junlu Sheng*,&nbsp;, and ,&nbsp;Yongbo Yao*,&nbsp;","doi":"10.1021/acsanm.5c03760","DOIUrl":"https://doi.org/10.1021/acsanm.5c03760","url":null,"abstract":"<p >Fluorine-free waterproof and breathable membranes (WBMs) with exceptional protective capabilities, efficient moisture-vapor transmission, and environmentally benign characteristics have demonstrated indispensable application value in multiple fields. Herein, polyacrylonitrile/isophorone diisocyanate/aminosilicone oil (PAN/IPDI/ASO) nanofibrous membranes were prepared by electrospinning and coated with a fluorine-free hydrophobic coating. The obtained composite membranes exhibited excellent comprehensive properties with a hydrostatic pressure of 64.4 kPa, a water vapor permeability (WVP) of 6.4 kg·m^–2·d^–1, and a tensile strength of 11.6 MPa. Moreover, the PAN/IPDI-1/ASO-2 nanofibrous membranes maintained their water repellency and mechanical property under strong acidic and alkaline conditions and remained hydrophobic even after 60 abrasion cycles and 30 min of water ultrasonic cleaning. These fluorine-free and environmentally friendly PAN/IPDI/ASO WBMs have broad application prospects in outdoor sports equipment, medical protection, and other fields.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19609–19618"},"PeriodicalIF":5.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Spike Geometry on Electromagnetic Field Enhancement and the Linear and Nonlinear Plasmonic Properties of Gold Nanourchins","authors":"Domantas Peckus*,&nbsp;, ,&nbsp;Fatima Albatoul Kasabji,&nbsp;, ,&nbsp;Maziar Moussavi,&nbsp;, ,&nbsp;Loic Vidal,&nbsp;, ,&nbsp;Hana Boubaker,&nbsp;, ,&nbsp;Asta Tamulevičienė,&nbsp;, ,&nbsp;Arnaud Spangenberg,&nbsp;, ,&nbsp;Tomas Tamulevičius,&nbsp;, ,&nbsp;Joel Henzie*,&nbsp;, ,&nbsp;Karine Mougin*,&nbsp;, and ,&nbsp;Sigitas Tamulevičius*,&nbsp;","doi":"10.1021/acsanm.5c03297","DOIUrl":"https://doi.org/10.1021/acsanm.5c03297","url":null,"abstract":"<p >Wet-chemistry-synthesized gold nanourchins (Au NUs), characterized by spiky morphologies with spherical cores, exhibit complex and geometry-dependent plasmonic field enhancement properties distinct from those of symmetrical nanostructures. While plasmon hybridization and mode coupling in branched nanostructures have been broadly studied, the specific optical behavior of Au NUs─particularly regarding spike length distribution and ultrafast dynamics─remains underexplored. This study investigates the steady-state and transient absorption spectra of Au NUs with 50–80 nm cores and 5–20 nm spikes, revealing multiple resonance bands. Transient absorption spectroscopy at various excitation wavelengths confirmed the presence of distinct resonances. Electromagnetic simulations based on TEM tomography-inspired models identified two key extinction bands: a green-wavelength dark mode resonance and a red/nIR spike-induced resonance attributed to the lightning rod effect. Simulations further showed that short, uniformly distributed spikes (aspect ratio ≤1) weakly excite dark resonances, while longer spikes (aspect ratio &gt;1) induce hybridized longitudinal resonances and significant redshifts. Broad spike length distributions result in multiple coexisting resonances, aligning with experimental extinction spectra. A preliminary surface-enhanced Raman scattering study using 2-naphthalene thiol confirmed stronger enhancement for long-spiked Au NUs under 785 nm excitation, validating the field enhancement potential of the identified resonances.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19322–19332"},"PeriodicalIF":5.5,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facet-Controlled Synthesis of Nanoporous Metal–Organic Frameworks for Enhanced Activity of Surface-Immobilized Thrombin","authors":"Zhanglong Zhu,&nbsp;, ,&nbsp;Yang Zou,&nbsp;, ,&nbsp;Haiyan Ju,&nbsp;, and ,&nbsp;Yonggang Lv*,&nbsp;","doi":"10.1021/acsanm.5c04107","DOIUrl":"https://doi.org/10.1021/acsanm.5c04107","url":null,"abstract":"<p >Thrombin is central to the process of the coagulation cascade reaction, and its activity directly affects fibrin formation and final hemostasis. Nanomaterial-based hemostatic agents have exhibited excellent performance in hemorrhage management. The unique exposed crystal facets of nanomaterials play a crucial role in catalytic reactions but are often overlooked. Here, a series of nanoscale zeolitic imidazolate frameworks (denoted as ZIF-8/C with exposed (100) facet, ZIF-8/TRD with mixed exposed (100) and (110) facets, and ZIF-8/RD with exposed (110) facet) were successfully synthesized with crystal facets progressively evolving from (100) to (110), which serve as catalytic platforms to accelerate the process of the coagulation cascade reaction by modulating the activity of thrombin immobilized on their surfaces. ZIF-8/TRD exhibited a striking catalytic capacity with at least 13-fold higher levels of thrombin expression compared with natural platelet-based physiological processes. Molecular dynamics (MD) simulations revealed that the special mixed-facet environment increased substrate accessibility and exhibited a more stable active site conformation. This work focuses on the regulation of enzymatic reactions carried out on the protein corona of the host material by crystal facets, paving the way for the design of hemostatic agents with enhanced thrombin activity.</p>","PeriodicalId":6,"journal":{"name":"ACS Applied Nano Materials","volume":"8 40","pages":"19645–19657"},"PeriodicalIF":5.5,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}