Acta Naturae最新文献_第8页

The Unique Genome of the Virus and Alternative Strategies for its Realization. 病毒的独特基因组及其实现的替代策略。

IF 2 4区生物学

Acta Naturae Pub Date : 2023-04-01 DOI: 10.32607/actanaturae.11904

O P Zhirnov

引用次数: 0

EGFR Suppression Inhibits the Sphere Formation of MCF7 Cells Overexpressing EGFR. EGFR抑制抑制过度表达EGFR的MCF7细胞的球体形成。

IF 2 4区生物学

Acta Naturae Pub Date : 2023-04-01 DOI: 10.32607/actanaturae.17857

D D Novak, O S Troitskaya, A A Nushtaeva, M V Zhilnikova, V A Richter, M I Meschaninova, O A Koval

{"title":"EGFR Suppression Inhibits the Sphere Formation of MCF7 Cells Overexpressing EGFR.","authors":"D D Novak, O S Troitskaya, A A Nushtaeva, M V Zhilnikova, V A Richter, M I Meschaninova, O A Koval","doi":"10.32607/actanaturae.17857","DOIUrl":"10.32607/actanaturae.17857","url":null,"abstract":"The epidermal growth factor receptor (EGFR) is an oncogenic tyrosine kinase that is involved in tumor initiation and progression, making EGFR inhibitors and monoclonal antibodies to this receptor essential for anti-tumor therapy. We have previously shown that EGFR transgene expression in the human breast adenocarcinoma cell line MCF7 (MCF7-EGFR) stimulates the 3D spheroid-like growth. The primary focus of our present work was to investigate whether EGFR inhibition could affect the assembly of spheroids or lead to the destruction of pre-existing spheroids. We compared the effects of anti-EGFR siRNA, the anti-EGFR monoclonal antibody cetuximab, and the tyrosine kinase inhibitor AG1478 on dissociated and spheroid MCF7-EGFR cells. MCF7-EGFR cells were found to have a 2.5-fold higher sensitivity towards the cytotoxic effects of cetuximab and AG1478 compared with the parental MCF7 cell line. The suppression of EGFR mRNA with siRNA was found to reduce the sphere formation, whereas treating the pre-existing spheroids had no such effect. Treatment of dissociated spheroids with cetuximab and AG1478 was also found to inhibit the MCF7-EGFR sphere formation. We suggest that EGFR expression is important, at least, during the spheroid formation stage. The transition of a MCF7wt adherent cell culture to MCF7-EGFR spheroids was accompanied by a considerable increase in N-cadherin adhesion proteins. The level of N-cadherin decreased when MCF7-EGFR cells were treated with siRNA and cetuximab. Thus, we have demonstrated that N-cadherin is involved in the EGFR-dependent formation of MCF7-EGFR spheroids. Accordingly, MCF7-EGFR spheroids can be considered a suitable model for studying aggressive hormone-positive breast tumors.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"15 2","pages":"59-69"},"PeriodicalIF":2.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10314626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Search for Inhibitors of Mycobacterium tuberculosis Transketolase in a Series of Sulfo-Substituted Compounds. 在一系列磺基取代化合物中寻找结核分枝杆菌转酮酶抑制剂。

IF 2 4区生物学

Acta Naturae Pub Date : 2023-04-01 DOI: 10.32607/actanaturae.15709

I V Gushchina, D K Nilov, T A Shcherbakova, S M Baldin, V K Švedas

引用次数: 0

Gene Therapy for Cystic Fibrosis: Recent Advances and Future Prospects. 囊性纤维化的基因治疗：最新进展和未来展望。

IF 2 4区生物学

Acta Naturae Pub Date : 2023-04-01 DOI: 10.32607/actanaturae.11708

M A Lomunova, P M Gershovich

{"title":"Gene Therapy for Cystic Fibrosis: Recent Advances and Future Prospects.","authors":"M A Lomunova, P M Gershovich","doi":"10.32607/actanaturae.11708","DOIUrl":"10.32607/actanaturae.11708","url":null,"abstract":"Gene replacement therapies are novel therapeutic approaches that seek to tackle hereditary diseases caused by a congenital deficiency in a particular gene, when a functional copy of a gene can be delivered to the cells and tissues using various delivery systems. To do this, viral particles carrying a functional copy of the gene of interest and various nonviral gene delivery systems, including liposomes, nanoparticles, etc., can be used. In this review, we discuss the state of current knowledge regarding the molecular mechanisms and types of genetic mutations that lead to cystic fibrosis and highlight recent developments in gene therapy that can be leveraged to correct these mutations and to restore the physiological function of the carrier protein transporting sodium and chlorine ions in the airway epithelial cells. Restoration of carrier protein expression could lead to the normalization of ion and water transport across the membrane and induce a decrease in the viscosity of airway surface fluid, which is one of the pathological manifestations of this disease. This review also summarizes recently published preclinical and clinical data for various gene therapies to allow one to make some conclusions about future prospects for gene therapy in cystic fibrosis treatment.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"15 2","pages":"20-31"},"PeriodicalIF":2.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9993392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Connectivity of the Brain in the Light of Chemogenetic Modulation of Neuronal Activity. 从神经元活动的化学遗传学调节看大脑的连接性。

IF 2 4区生物学

Acta Naturae Pub Date : 2023-04-01 DOI: 10.32607/actanaturae.11895

N N Dygalo

{"title":"Connectivity of the Brain in the Light of Chemogenetic Modulation of Neuronal Activity.","authors":"N N Dygalo","doi":"10.32607/actanaturae.11895","DOIUrl":"10.32607/actanaturae.11895","url":null,"abstract":"Connectivity is the coordinated activity of the neuronal networks responsible for brain functions; it is detected based on functional magnetic resonance imaging signals that depend on the oxygen level in the blood (blood oxygen level-dependent (BOLD) signals) supplying the brain. The BOLD signal is only indirectly related to the underlying neuronal activity; therefore, it remains an open question whether connectivity and changes in it are only manifestations of normal and pathological states of the brain or they are, to some extent, the causes of these states. The creation of chemogenetic receptors activated by synthetic drugs (designer receptors exclusively activated by designer drugs, DREADDs), which, depending on the receptor type, either facilitate or, on the contrary, inhibit the neuronal response to received physiological stimuli, makes it possible to assess brain connectivity in the light of controlled neuronal activity. Evidence suggests that connectivity is based on neuronal activity and is a manifestation of connections between brain regions that integrate sensory, cognitive, and motor functions. Chemogenetic modulation of the activity of various groups and types of neurons changes the connectivity of the brain and its complex functions. Chemogenetics can be useful in reconfiguring the pathological mechanisms of nervous and mental diseases. The initiated integration, based on the whole-brain connectome from molecular-cellular, neuronal, and synaptic processes to higher nervous activity and behavior, has the potential to significantly increase the fundamental and applied value of this branch of neuroscience.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"15 2","pages":"4-13"},"PeriodicalIF":2.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10295678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell Therapy: A New Technology for Cerebral Circulation Restoration after Ischemia/Reperfusion. 细胞治疗：脑缺血/再灌注后恢复脑循环的新技术。

IF 2 4区生物学

Acta Naturae Pub Date : 2023-04-01 DOI: 10.32607/actanaturae.14338

I B Sokolova, O P Gorshkova

{"title":"Cell Therapy: A New Technology for Cerebral Circulation Restoration after Ischemia/Reperfusion.","authors":"I B Sokolova, O P Gorshkova","doi":"10.32607/actanaturae.14338","DOIUrl":"10.32607/actanaturae.14338","url":null,"abstract":"Cell therapy with mesenchymal stem cells (MSCs) may be a promising technique for cerebral blood flow restoration after transient ischemia. Before a practical application of the cell material, 7-9 days are required for its cultivation. We studied the efficacy of human MSC (hMSC) transplantation performed 7 days after cerebral ischemia/reperfusion (I/R) to help recover cerebral circulation. The intravital micrograph technique was used to comparatively evaluate the vasculature density in the pia mater and the reactivity of the pial arteries in response to acetylcholine (ACh) in rats after I/R (clamping of both carotid arteries and a simultaneous decrease in and strict maintenance of the mean BP at 45 ± 2 mm Hg for 12 min) and with/without hMSC transplantation. Perfusion (P) in the sensorimotor cortex was assessed using laser dopplerography. After 14 and 21 days, the vasculature density in I/R-affected rats was 1.2- to 1.4-fold and 1.2- to 1.3-fold lower, respectively, than that in the controls. The number of ACh-dilated arteries decreased 1.6- to 1.9-fold and 1.2- to 1.7-fold 14 and 21 days after I/R, respectively. After 21 days, the P level decreased 1.6-fold, on average. Administration of hMSCs on day 7 after I/R resulted in complete recovery of the vasculature density by day 14. ACh-mediated dilatation fully recovered only in arteries of less than 40 μm in diameter within 21 days. After 21 days, the P level was 1.2-fold lower than that in the controls but significantly higher than that in rats after I/R without hMSCs. Delayed administration of MSCs after a transient cerebral ischemic attack affords the time for the procedures required to prepare cell material for transplantation and provides a good therapeutic response in the pial microvasculature.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"15 2","pages":"75-80"},"PeriodicalIF":2.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10395779/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10314633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MALAT1 Long Non-coding RNA and Its Role in Breast Carcinogenesis. MALAT1长非编码RNA及其在乳腺癌发生中的作用。

IF 2 4区生物学

Acta Naturae Pub Date : 2023-04-01 DOI: 10.32607/actanaturae.11905

M M Tsyganov, M K Ibragimova

引用次数: 0

Les manchons de mousse corticoles, un micro-habitat riche en Invertébrés

IF 2 4区生物学

Acta Naturae Pub Date : 2023-03-22 DOI: 10.5852/naturae2023a4

F. Darinot, Robin Letscher, J. Geoffroy, S. Guillocheau, B. Dodelin

{"title":"Les manchons de mousse corticoles, un micro-habitat riche en Invertébrés","authors":"F. Darinot, Robin Letscher, J. Geoffroy, S. Guillocheau, B. Dodelin","doi":"10.5852/naturae2023a4","DOIUrl":"https://doi.org/10.5852/naturae2023a4","url":null,"abstract":"Un inventaire de la faune invertébrée vivant dans le sol et dans les mousses corticoles a été mené dans deux forêts, situées dans la Réserve naturelle nationale du Marais de Lavours et la Réserve naturelle régionale de la Galerie du Pont des Pierres (Département de l’Ain). Au total, 32 338 spécimens ont été collectés, correspondant à trois phylums (Mollusques, Annélides et Arthropodes) et 24 groupes taxonomiques. À surface égale, les manchons de mousses comportent davantage d’Invertébrés que le sol. Les Acariens sont très majoritaires et plus abondants que les Collemboles, eux-mêmes dix fois plus nombreux que les Hyménoptères (Fourmis), les Coléoptères, les Araignées et les Cloportes. En hiver, le peuplement du sol et des mousses à Lavours semble nettement différent de celui d’été et des peuplements du Pont des Pierres. Il existe un gradient d’abondance depuis le sol jusqu’à la hauteur d’environ un mètre, que l’on retrouve également en été, mais qui fait défaut au Pont des Pierres. Les inondations hivernales sont probablement responsables de cette répartition altitudinale des Invertébrés à Lavours, où les manchons muscicoles jouent un rôle de refuge pour les Arthropodes. Plus généralement, les coussins de mousse fonctionnent en annexe de la litière du sol avec un continuum d’espèces.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"76 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82572401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apports d’un dispositif intégré « Communauté-Population-Sol » pour évaluer l’effet des grands herbivores sur les écosystèmes 综合社区-种群-土壤系统对评估大型食草动物对生态系统影响的贡献

IF 2 4区生物学

Acta Naturae Pub Date : 2023-03-08 DOI: 10.5852/naturae2023a3

I. Castañeda, L. Callede, Emmanuel Corcket

{"title":"Apports d’un dispositif intégré « Communauté-Population-Sol » pour évaluer l’effet des grands herbivores sur les écosystèmes","authors":"I. Castañeda, L. Callede, Emmanuel Corcket","doi":"10.5852/naturae2023a3","DOIUrl":"https://doi.org/10.5852/naturae2023a3","url":null,"abstract":"Les enjeux de conservation de la biodiversité ainsi que les besoins d’amélioration des connaissances sur les interactions entre grands herbivores et écosystèmes nécessitent la mise en place de dispositifs de suivis standardisés. Dans le contexte du programme de conservation de la Vache Marine landaise et de la gestion des prairies humides à Molinie du plateau landais (région Nouvelle-Aquitaine, France), un dispositif expérimental a été mise en place en 2019 pour le suivi des effets de la Vache Marine landaise dans le cadre de la conservation et la restauration des espaces naturels humides. La conception du dispositif, baptisée « Communauté-Population-Sol » (CPS) est novatrice et intègre trois composantes-clés de l’écosystème : la communauté végétale, la population végétale dominante et le sol, via des relevés exhaustifs de végétation vasculaire, des caractérisations de traits biologiques de la population végétale dominante (Molinia caerulea (L.) Moench) et des paramètres physico-chimiques du sol. Les mesures initiales effectuées dans le dispositif CPS montre une parfaite homogénéité quant aux propriétés initiales des placettes, avant l’effet différentiel de l’exclusion de pâturage. Aucune différence significative de structure de communauté végétale (biodiversité), de caractéristiques de la Molinie (nécromasse, traits foliaires) et de paramètres édaphiques (pH, carbone, nutriments) n’a été mise en évidence entre les modalités d’exclos et de pâturage à l’installation, ce qui garantit une absence de biais initial au niveau des placettes de suivi. Les dispositifs de type CPS ont pour vocation d’être généralisés et adaptés pour permettre une évaluation intégrée des mesures de gestion par le pastoralisme dans les espaces naturels, bien plus fine que les simples suivis de végétation.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"51 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89891675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quelle est la signification taxonomique d’un caractère morphologique particulier des chabots (Scorpaeniformes: Cottidae: Cottus sp.) du bassin versant de la Garonne ? 加隆河流域的棚子(Scorpaeniformes: Cottidae: Cottus sp.)的特殊形态特征的分类学意义是什么?

IF 2 4区生物学

Acta Naturae Pub Date : 2023-02-15 DOI: 10.5852/naturae2023a2

P. Defontaines

{"title":"Quelle est la signification taxonomique d’un caractère morphologique particulier des chabots (Scorpaeniformes: Cottidae: Cottus sp.) du bassin versant de la Garonne ?","authors":"P. Defontaines","doi":"10.5852/naturae2023a2","DOIUrl":"https://doi.org/10.5852/naturae2023a2","url":null,"abstract":"La taxonomie des chabots (Cottus spp.) du territoire français n’est pas définitivement fixée, les caractères morphologiques utilisés pour différencier les espèces étant souvent variables et ne correspondant pas toujours avec les variations moléculaires. C’est particulièrement vrai dans le bassin versant de la Garonne où plusieurs espèces sont répertoriées, mais avec beaucoup d’incertitude sur leur répartition et leur taxonomie. Un caractère particulier a été découvert chez certains chabots de ce bassin : ils ont trois rayons aux nageoires pelviennes, contre quatre rayons chez toutes les espèces européennes décrites aujourd’hui. Cet article étudie la répartition et l’écologie des Chabots de ce morphotype pour les comparer à celles des chabots du morphotype habituel à quatre rayons aux nageoires pelviennes. Les Chabots à trois rayons occupent essentiellement des rivières de basse altitude en terrain karstique. Leurs populations peuvent être réunies en un seul ensemble par l’aval du bassin-versant, ce qui suggère une origine géographique unique. Ceux à quatre rayons ne semblent pas inféodés à un habitat particulier et se trouvent fréquemment sur terrain cristallin ou magmatique dans des rivières prenant naissance en altitude. Ces différences biogéographiques et écologiques suggèrent que le caractère « trois rayons aux nageoires pelviennes » est un caractère permettant de définir une lignée phylogénétique particulière, possible nouvelle espèce. Des études morphologiques et génétiques sont nécessaires pour vérifier cette hypothèse. Si le Chabot à trois rayons est bien une nouvelle espèce, elle nécessiterait alors des niveaux de protection spécifiques compte tenu de ses faibles effectifs, du fractionnement de ses populations et des menaces auxquelles elle est confrontée.","PeriodicalId":6989,"journal":{"name":"Acta Naturae","volume":"11 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2023-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73163314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0