Acta biologica et medica Germanica最新文献_第4页

Inhibition of soluble guanylate cyclase activity by citrate. 柠檬酸对可溶性鸟苷酸环化酶活性的抑制作用。

Acta biologica et medica Germanica Pub Date : 1982-01-01

P Karczewski, E G Krause

引用次数: 0

Low dose streptozotocin diabetes after partial pancreatectomy in dogs. Histological findings in a new type of experimental diabetes. 狗部分胰腺切除术后低剂量链脲佐菌素糖尿病。一种新型实验性糖尿病的组织学发现。

Acta biologica et medica Germanica Pub Date : 1982-01-01

E J Freyse, H Hahn von Dorsche, U Fischer

{"title":"Low dose streptozotocin diabetes after partial pancreatectomy in dogs. Histological findings in a new type of experimental diabetes.","authors":"E J Freyse, H Hahn von Dorsche, U Fischer","doi":"","DOIUrl":"","url":null,"abstract":"Permanent diabetes was produced in 16 out of 55 dogs by partial pancreatectomy (77% of the calculated organ weight) and simultaneous infusion of 2 mg/kg streptozotocin into the superior pancreaticoduodenal artery. The animals exhibited hyperglycemia, absolute lack of endogenous B-cell function, and ketosis, but no exocrine pancreatic insufficiency. 21 animals needed up to 7 additional subsequent intravenous streptozotocin injections (15 mg/kg each at intervals of 3 days). In 18 animals the procedure failed to render them diabetic; they died mainly from toxic effects of the drug. There were severe pathohistological changes in all streptozotocin-treated animals. Besides the well known alterations of the islets of Langerhans, lymphocytic inflammations were found in numerous organs including the exocrine pancreas. In most cases they were combined with degenerative changes of the organ parenchyma, particularly in kidney and liver. These findings were not correlated to the sex of the animals, to the occurrence and severity if diabetes, to the time of survival, or to the streptozotocin dose applied. But they were obviously related to the clinical picture existing besides diabetes. It is concluded that the model of experimental diabetes presented might be useful in a carnivorous big animal species but that toxic streptozotocin effects are to be expected when the dose administered exceeds 2 mg/kg.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 12","pages":"1203-10"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17295778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Quantitative determination of factor VIII antigen with an enzyme immunoassay]. [酶免疫分析法定量测定因子VIII抗原]。

Acta biologica et medica Germanica Pub Date : 1982-01-01

W Schössler, M Stepanauskas, C Dittrich, M von Chr Eichhorn

引用次数: 0

[Activity of gluconeogenetic enzymes in the kidney of pigs during pre- and post-natal development]. [猪产前和产后肾脏糖异生酶的活性]。

Acta biologica et medica Germanica Pub Date : 1982-01-01

E Grün, K Pfüller, D Heyne, C Weber

{"title":"[Activity of gluconeogenetic enzymes in the kidney of pigs during pre- and post-natal development].","authors":"E Grün, K Pfüller, D Heyne, C Weber","doi":"","DOIUrl":"","url":null,"abstract":"In pig fetuses (19 of 8 dams) developed by Caesarean section the dry matter and protein content of the kidneys and their PEPCK activity remain constant during the last third (from 80th to 112th day) of gestation. After birth the dry matter content of the kidneys rises slowly, but their protein content remarkably. In the kidneys of suckling piglets (17 animals of 3 offsprings) the FDPase activity remains at the same level from birth to the 9th day of life, while in the same time the G6Pase activity rises 1.5-2 times. In the kidneys of newborn piglets the total PEPCK activity increases 3-4 times and the activity of the cytosolic enzyme 2-3 times during the first 12 hours of life. At the end of the first week of life the total PEPCK activity decreases by one-third, while the activity of the cytosolic enzyme remains stable. In the kidneys of slaughter pigs (n = 7) the dry matter content and the FDPase activity are significantly higher, the protein content and the G6Pase activity are the same as in the kidneys of piglets. The total PEPCK activity is one-half, the activity of the cytosolic enzyme one-third lower than in the kidneys of piglets. In the kidneys of adult pigs the PEPCK activity is localized to equal parts in the cytosol and in the mitochondria, but in some development stages the mitochondrial part exceeds that of the cytosol. In adult pigs the PEPCK activity of the renal cortex is 2.5-3 times higher than that of the renal medulla.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 10","pages":"861-71"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17365216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Receptor mediated endocytosis of hemoglobin-haptoglobin, galactosylated serum albumin and polymeric IgA by the liver. 受体介导的肝内吞血红蛋白-接触珠蛋白、半乳糖化血清白蛋白和聚合IgA。

Acta biologica et medica Germanica Pub Date : 1982-01-01

J N Limet, J Quintart, C Otte-Slachmuylder, Y J Schneider

{"title":"Receptor mediated endocytosis of hemoglobin-haptoglobin, galactosylated serum albumin and polymeric IgA by the liver.","authors":"J N Limet, J Quintart, C Otte-Slachmuylder, Y J Schneider","doi":"","DOIUrl":"","url":null,"abstract":"Labelled hemoglobin-haptoglobin (ham-hap), galactosylated serum albumin (gal-SA) and polymeric immunoglobulin A (p IgA) were injected intravenously to rats or mice. The labels disappeared from the plasma with a half-time of about 5 min and were almost entirely found associated with the liver where degradation products progressively appear. The uptake of hem-hap and gal-SA are partially saturable as a function of the plasmatic concentration and the uptake of gal-SA can be completely inhibited by the simultaneous injection of asialofetuin. About 45 min after injection to rats, labelled material appears in the bile in amounts corresponding to 3.9% of the injected dose (hem-hap), 2.8% (gal-SA) and 60.1% (p IgA). The molecular weight of the labelled material transferred into the bile has been characterized: it consists almost entirely of intact IgA and for about 60% of intact hem-hap and gal-SA. Cell fractionation experiments indicate that 4 min after injection, the label is associated with components which equilibrate around a density of 1.13 g/cm3 and which dissociate from marker enzymes of Golgi complex, plasma membrane and lysosomes. Longer times after injection (from 20 min for hem-hap and gal-SA to 1 h for p IgA) labelled material appears, within lysosomes. To explain all these data, we suggest that after binding to plasma membrane receptors, the ligands are rapidly interiorized into pinocytic vesicles which fuse with lysosomes. Most of the hem-hap and gal-SA molecules but only part of p IgA would be released and subsequently digested; these vesicles would dissociate from lysosomes and fuse with the biliary membrane where the molecules still bound to the membrane of the vesicles would be detached and excreted into the bile.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 1","pages":"113-24"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17349783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Use of thermitase in the cultivation of embryonal mouse fibroblasts]. 热丝酶在胚胎性小鼠成纤维细胞培养中的应用

Acta biologica et medica Germanica Pub Date : 1982-01-01

C Frömmel, A Böhmer

引用次数: 0

Induction of immunological tolerance against sheep red blood cells in A/J mice by maternal immunization. 母系免疫诱导A/J小鼠对绵羊红细胞免疫耐受的研究。

Acta biologica et medica Germanica Pub Date : 1982-01-01

S Schröder, H Schäffner, H Ambrosius

引用次数: 0

[Lipid concentrations in liver cell fractions in the rat in diet induced obesity]. [饮食诱导肥胖大鼠肝细胞组分的脂质浓度]。

Acta biologica et medica Germanica Pub Date : 1982-01-01

D Stauske, W Haude

{"title":"[Lipid concentrations in liver cell fractions in the rat in diet induced obesity].","authors":"D Stauske, W Haude","doi":"","DOIUrl":"","url":null,"abstract":"The changes of lipid parameters induced in male Wistar rats by feeding a high-fat diet for several weeks were studied in cell fractions of the liver. For this purpose subcellular fractionations of liver tissue from 12-week-old animals receiving food containing, respectively, 3% fat (controls) and 50% fat, were performed by means of differential centrifugation, and the lipids in the cell fractions were determined quantitatively. The levels of triglycerides and cholesteryl esters both in total liver and in the cell fractions had risen several fold against the controls. Most of the accumulated lipids were retrieved in the cytosolic supernatant. In contrast, for the phospholipids and the free cholesterol only a slight increase in free cholesterol was observed in total liver, and increases of both lipids had occurred in the cytosolic supernatant, while the particulate fractions showed no alterations. It is pointed out in the discussion that the accumulation of triglycerides and cholesteryl esters in the liver largely concerns the particulate fractions too (observed so far in the literature only for single particulate fractions), which might be of interest for the function of the cell nucleus, the mitochondria and the endoplasmatic reticulum. The increase of phospholipids and free cholesterol in the supernatant has to be considered not only as a passive process of deposition, but could represent necessary contributions to the building up of lipid droplets in the cytosol during deposition of triglycerides and cholesterol in this region of the cell. The studies confirm that the fatty degeneration of the liver, true, is always the result of triglyceride accumulation but that, under certain conditions, cholesterol is also enriched in the form of its esters.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 7-8","pages":"665-74"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18163795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acetylcholine concentrations in the developing brain appear to affect emotionality and mental capacity in later life. 发育中的大脑中的乙酰胆碱浓度似乎会影响晚年的情绪和智力。

Acta biologica et medica Germanica Pub Date : 1982-01-01

G Dörner, R Bluth, R Tönjes

引用次数: 0

Discrimination of several classes of nonhistone proteins in chromatin fractions from liver and thymus nuclei of rats. 大鼠肝脏和胸腺核染色质组分中几种非组蛋白的鉴别。

Acta biologica et medica Germanica Pub Date : 1982-01-01

A Weihe, G Schmidt, C U von Mickwitz, R Lindigkeit

{"title":"Discrimination of several classes of nonhistone proteins in chromatin fractions from liver and thymus nuclei of rats.","authors":"A Weihe, G Schmidt, C U von Mickwitz, R Lindigkeit","doi":"","DOIUrl":"","url":null,"abstract":"Nonhistone proteins (NHPs) of salt-soluble chromatin (Chromatin S) and of the residual nuclei (Chromatin P) from rat liver and thymus were studied by SDS-polyacrylamide gel electrophoresis. The two chromatin fractions of the liver showed significant differences in their NHP patterns with most of the hnRNP and matrix proteins occurring in Chromatin P. In accordance with the low protein content of thymus nuclei, the corresponding thymus fractions exhibited electrophoretic patterns with a markedly lower amount of NHPs than in liver. Chromatin P from thymus, in contrast to the liver fraction, revealed only a very low content of hnRNP-specific proteins of molecular weight 30,000-40,000 (30 K to 40 K) (informosomal proteins) consistent with the significantly lower RNA content of thymus nuclei. In the region of the matrix proteins (60-75 K) Chromatin P showed only two bands of about 64 K and 73 K in thymus, whereas in liver five strong bands at 64 K, 66 K, 69 K, 73 K, and 75 K were found. RNase digestion was employed to discriminate hnRNP-specific protein from \"real\" chromosomal NHPs. At least about 65% and 25% of the NHPs from Chromatin P and S of liver, respectively, were found to be RNP-specific. The two chromatin fractions were further fractionated by sucrose gradient centrifugation and isopycnic banding in metrizamide. After centrifugation the main peaks, both of Chromatin S and P, contained only minor amounts of NHPs with a predominating protein of 38 K. By the centrifugation procedures described in this paper, a small subfraction of chromatin could be separated which was enriched in newly synthesized RNA, informosomal proteins, matrix- and other high molecular weight proteins. This subfraction might be related to transcriptionally active chromatin.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 7-8","pages":"609-24"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18163911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0