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Acta biologica et medica Germanica最新文献

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The application of a sepharose bead immunofluorescence assay and a solid-phase radioimmunoassay to the bovine leukemia virus system. 糖珠免疫荧光法和固相放射免疫法在牛白血病病毒系统中的应用。
Acta biologica et medica Germanica Pub Date : 1982-01-01
H Fiebach, W Uckert, B Micheel
{"title":"The application of a sepharose bead immunofluorescence assay and a solid-phase radioimmunoassay to the bovine leukemia virus system.","authors":"H Fiebach,&nbsp;W Uckert,&nbsp;B Micheel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Several fluorescence assays with bovine leukemia virus (BLV) conjugated to activated Sepharose 4B were used for the detection of BLV and anti-BLV antibodies. These tests were compared with a solid-phase radioimmunoassay and found to be in the same sensitivity range. Sepharose bead immunofluorescence assay and solid-phase radioimmunoassay can be applied to the diagnosis of BLV infection in cattle.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 7-8","pages":"689-94"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17354942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new procedure for the purification of proteinase B from baker's yeast and interaction of the purified enzyme with a specific inhibitor. 从面包酵母中纯化蛋白酶B的新方法及纯化酶与特定抑制剂的相互作用。
Acta biologica et medica Germanica Pub Date : 1982-01-01
K Huse, G Kopperschläger, E Hofmann
{"title":"A new procedure for the purification of proteinase B from baker's yeast and interaction of the purified enzyme with a specific inhibitor.","authors":"K Huse,&nbsp;G Kopperschläger,&nbsp;E Hofmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Proteinase B from baker's yeast was purified to homogeneity by applying affinity chromatography with D,L-tyrosine ethyl ester as ligand. The molecular parameters of the product are similar to those reported formerly by other groups. A different form of proteinase B is isolated if affinity chromatography is replaced by CM-cellulose chromatography and gel filtration. In this case a peptide tightly associated with the enzyme was found to occur. This could be identified as an inhibitor fragment produced by limited proteolysis of a proteinase B bound inhibitor induced by proteinase A.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 11","pages":"991-1002"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17815708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Pathogenesis and hemodynamic significance of veno-arterial reaction in diabetic microcirculation disorders]. [糖尿病微循环疾病静脉-动脉反应的发病机制及血流动力学意义]。
Acta biologica et medica Germanica Pub Date : 1982-01-01
S Kobylinski
{"title":"[Pathogenesis and hemodynamic significance of veno-arterial reaction in diabetic microcirculation disorders].","authors":"S Kobylinski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In human diabetes mellitus, functional microangiopathy starts with venule dilatation, the cause of which is unknown. The results of comparative measurements of animals with alloxan diabetes verify also an increase in the arterio-venous calibre relation. As studied vital-microscopically, these alterations are due to venule congestion and passive dilatation as a consequence or red cell aggregation and an increase in the viscosity of blood. The increase of the flow resistance on the postcapillary side depends only on rheological but not on nervous-humoral factors, which leads by means of a veno-arterial reaction to increased constrictions of the arterioles.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 5","pages":"457-64"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18150569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antihypertensive drugs alter the production and the ratio of prostaglandins E and F in the organs of spontaneously hypertensive rats. 降压药可改变自发性高血压大鼠各器官中前列腺素E、F的生成及比值。
Acta biologica et medica Germanica Pub Date : 1982-01-01
C Taube, H U Block, W Förster
{"title":"Antihypertensive drugs alter the production and the ratio of prostaglandins E and F in the organs of spontaneously hypertensive rats.","authors":"C Taube,&nbsp;H U Block,&nbsp;W Förster","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 5","pages":"477-85"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18150570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Structural and functional characteristics of IgG1- and IgG2-antibody subclasses in the guinea pig. 2. Production and testing of specific antisera against IgG1 and IgG2-antibody subclasses and against Fc-, Fc'- and Fab-papain fragments of IgG2-antibodies in guinea pigs]. 豚鼠IgG1-和igg2抗体亚类的结构和功能特征。2. 豚鼠抗IgG1和igg2抗体亚类和抗igg2抗体Fc-、Fc'-和fab -木瓜蛋白酶片段特异性抗血清的生产和检测[j]。
Acta biologica et medica Germanica Pub Date : 1982-01-01
K Drössler, D Hädge, H Ambrosius
{"title":"[Structural and functional characteristics of IgG1- and IgG2-antibody subclasses in the guinea pig. 2. Production and testing of specific antisera against IgG1 and IgG2-antibody subclasses and against Fc-, Fc'- and Fab-papain fragments of IgG2-antibodies in guinea pigs].","authors":"K Drössler,&nbsp;D Hädge,&nbsp;H Ambrosius","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Rabbit antisera against specifically purified guinea pig IgG1 and IgG2 antibodies and against several papain-fragments of IgG2 were produced. These antisera were made specific by absorption with purified immunoglobulin of the other subclass and assayed for monospecificity by two immunological tests. In the macrophage rosette inhibition test, only the anti-IgG2 and the anti-Fab (IgG2) sera were effective. Inhibition of the rosette formation could be obtained at dilutions up to 1:100 or higher. Attempts to induce passive cutaneous anaphylaxis in the guinea pig skin after sensibilization with homocytotropic antibodies were evidently positive only with rabbit anti-guinea pig IgG1 serum. Anti-IgG2 and anti-IgG2 Fab sera revealed a positive reaction in higher concentrated forms (1:2 or 1:5 diluted) only.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 4","pages":"393-400"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18138701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The in vitro effect of horse anti-human lymphocyte globulin on the electrophoretic mobility of human lymphocytes and platelets. 马抗人淋巴细胞球蛋白对人淋巴细胞和血小板电泳迁移率的体外影响。
Acta biologica et medica Germanica Pub Date : 1982-01-01
E Knippel, S Preussner, W Schütt, U Thomaneck, R Claus, J Rychly
{"title":"The in vitro effect of horse anti-human lymphocyte globulin on the electrophoretic mobility of human lymphocytes and platelets.","authors":"E Knippel,&nbsp;S Preussner,&nbsp;W Schütt,&nbsp;U Thomaneck,&nbsp;R Claus,&nbsp;J Rychly","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In accordance with earlier results the electrophoretic mobility of lymphocytes is decreased by horse anti-human lymphocyte globulin (AHLG), which correlates with the results of the rosette inhibition test. Under the influence of AHLG, the distribution of the electrophoretic mobilities of lymphocytes shows a characteristic change. In some of the AHLG samples, the antilymphocyte action is accompanied by an antithrombocyte effect. The resulting decrease in the electrophoretic mobility of platelets correlates with the thrombocytopenic effect in vivo. The variation of pH or ionic strength leads to changes of the AHLG effects. The in vivo influence of AHLG on the distribution of the electrophoretic mobility of peripheral blood lymphocytes and platelets is discussed.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 4","pages":"401-10"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18138702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The toxic A-chain of mistletoe lectin I: isolation and its effect on cell-free protein synthesis. 槲寄生凝集素毒性a链ⅰ的分离及其对无细胞蛋白合成的影响。
Acta biologica et medica Germanica Pub Date : 1982-01-01
H Franz, A Kindt, P Ziska, H Bielka, R Benndorf, L Venker
{"title":"The toxic A-chain of mistletoe lectin I: isolation and its effect on cell-free protein synthesis.","authors":"H Franz,&nbsp;A Kindt,&nbsp;P Ziska,&nbsp;H Bielka,&nbsp;R Benndorf,&nbsp;L Venker","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reduction of Sepharose bound mistletoe lectin I (ML I) is a convenient method for preparing A- and B-chains under mild conditions. The B-chain represents the D-galactose binding moiety. The A-chain shows a significant dose-dependent inhibition of protein synthesis in cell-free systems from rat liver and rabbit reticulocytes, while pure B-chain preparations do not affect ribosomal translation processes.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 4","pages":"K9-K16"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18139928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Participation of thalamic nuclei in the elaboration of conditioned avoidance reflexes of rats. VIII. Lesions of the nucleus posterior. 大鼠丘脑核参与条件回避反射的形成。8后核病变。
Acta biologica et medica Germanica Pub Date : 1982-01-01
F Klingberg, H Klingberg
{"title":"Participation of thalamic nuclei in the elaboration of conditioned avoidance reflexes of rats. VIII. Lesions of the nucleus posterior.","authors":"F Klingberg,&nbsp;H Klingberg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bilateral symmetric lesions of the posterior thalamic nucleus reduced the preoperatively learnt avoidance responses in Long-Evans hooded rats strongly. Postoperative acquisition of conditioned avoidance reflexes was rather low in each rat in a simple runway and impossible in an alternation task. Thresholds of reactions to pain (withdrawal of paws from the grid floor) were significantly increased. The lesioned rats had trouble to find the way out, as if they had difficulties to localize the source of punishment or to associate pain information with any other cues.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 5","pages":"497-500"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18153131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Visual placing responses impaired after mesencephalic lesions in the rat. 中脑损伤后大鼠视觉定位反应受损。
Acta biologica et medica Germanica Pub Date : 1982-01-01
P Mager, R Mager, F Klingberg
{"title":"Visual placing responses impaired after mesencephalic lesions in the rat.","authors":"P Mager,&nbsp;R Mager,&nbsp;F Klingberg","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 10","pages":"971-5"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18186242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of repeated carbon monoxide exposure to rats on cytochrome P-450 concentration and activities of monooxygenases in the liver. 多次一氧化碳暴露对大鼠肝脏细胞色素P-450浓度及单加氧酶活性的影响。
Acta biologica et medica Germanica Pub Date : 1982-01-01
D Pankow, F Schiller, D Müller
{"title":"Effect of repeated carbon monoxide exposure to rats on cytochrome P-450 concentration and activities of monooxygenases in the liver.","authors":"D Pankow,&nbsp;F Schiller,&nbsp;D Müller","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To determine the possible role of cytochrome P-450 in the adaptation response of an organism to chronic carbon monoxide exposure, rats received 4, 15 or 30 subcutaneous CO injections (7.2 mmol/kg body mass; maximal COHb concentration about 56%). The concentration of cytochrome P-450 is significantly reduced after 4 injections. This effect is no longer evident following 15 or 30 CO injections, which produced distinct increases of the total hemoglobin concentration. Ethylmorphine N-demethylation is distinctly inhibited after 4, 15 and 30 CO injections, whereas the initial inhibition of ethoxycoumarin O-deethylation disappears after 30 CO administrations. Ethoxyresorufin O-deethylation is only slightly influenced by CO.</p>","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 10","pages":"935-9"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18034648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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