Acta biologica et medica Germanica最新文献_第10页

Universal structural features of prokaryotic and eukaryotic ribosomal 5S RNA derived from comparative analysis of their sequences. 原核与真核核糖体5S RNA序列的比较分析。

Acta biologica et medica Germanica Pub Date : 1982-01-01

S Böhm, H Fabian, H Welfle

{"title":"Universal structural features of prokaryotic and eukaryotic ribosomal 5S RNA derived from comparative analysis of their sequences.","authors":"S Böhm, H Fabian, H Welfle","doi":"","DOIUrl":"","url":null,"abstract":"An extensive comparative analysis of more than fifty available sequences of ribosomal 5S RNA has been made. Both for prokaryotic and eukaryotic 5S RNA a generalized secondary structure is presented which is similar to that suggested by Nishikawa and Takemura modified in few positions only. Both generalized secondary structures contain five main helical regions and a high base-pairing content of about 65 +/- 5%. The general structural architecture of prokaryotic and eukaryotic 5S RNA molecules appears to be very similar with minor modifications within particular subgroups of organisms. Conserved and semiconserved nucleotides are accumulated in the single stranded parts of 5S RNA. Functional importance was suggested for some of these regions; other short conserved nucleotide stretches may be involved in the folding of 5S RNA molecules. In particular, we propose a tertiary base-pairing interaction between the universal invariant GUA sequence (positions 76-78 and 75-77 in prokaryotic and eukaryotic 5S RNA, respectively) and the complementary conserved CPuU sequence (positions 38-40 and 36-38) in a parallel manner. A molecular model of the 5S RNA of human KB cells was constructed, which verifies the proposed tertiary interaction, probably stabilizing the two neighboured helices E and D and a stacking arrangement of the bases in the sequence positions 67-108 (and 70-106) in eukaryotic (and prokaryotic) 5S RNAs, respectively.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18128832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autophagocytosis: freeze-fracture morphology, effects of vinblastine and influence of transcriptional and translational inhibitors. 自噬:冷冻断裂形态、长春花碱的作用以及转录和翻译抑制剂的影响。

Acta biologica et medica Germanica Pub Date : 1982-01-01

J Kovács, G Réz, A L Kovács, J Csák, G Zboray

引用次数: 0

Influence of anti-rheumatic drugs on the activity of collagenolytic cathepsin in hepatic culture. 抗风湿药物对肝组织蛋白酶活性的影响。

Acta biologica et medica Germanica Pub Date : 1982-01-01

E Kucharz, B Stawiarska, M Drozdz

引用次数: 0

Effect of linoleic acid-rich diet on blood pressure, lipids, catecholamines, and dopamine -beta-hydroxylase in spontaneously hypertensive rats. 富亚油酸饮食对自发性高血压大鼠血压、血脂、儿茶酚胺和多巴胺- β -羟化酶的影响。

Acta biologica et medica Germanica Pub Date : 1982-01-01

P Singer, V Moritz, D Förster, S Voigt, M Wirth, E Naumann, S Zimontkowski

{"title":"Effect of linoleic acid-rich diet on blood pressure, lipids, catecholamines, and dopamine -beta-hydroxylase in spontaneously hypertensive rats.","authors":"P Singer, V Moritz, D Förster, S Voigt, M Wirth, E Naumann, S Zimontkowski","doi":"","DOIUrl":"","url":null,"abstract":"Spontaneously hypertensive rats (SHR) and normotensive Wistar rats were fed a linoleic acid-rich (LAR) and -deficient (LAD) diet for 22 weeks, respectively. Although linoleic acid (LA) and arachidonic acid (AA) in serum and liver triglycerides markedly increased after a LAR diet, LA was significantly lower and AA was higher in SHR when compared to normotensive control rats. Thus, the percentage of both fatty acids remained different like in animals fed a commercial diet. On the contrary, in SHR and normotensive rats fed a LAD diet no differences in the LA and AA content could be found between the groups. In these rats, however, n-3 fatty acids (eicosapentaenoic and docosahexaenoic acids) in serum triglycerides were increased. Blood pressure, serum triglycerides and total cholesterol appeared unchanged, whereas HDL-cholesterol was increased after a LAR diet. Dopamine, adrenaline and noradrenaline content as well as dopamine-beta-hydroxylase activity were augmented in adrenal glands of SHR fed a LAR diet. In spite of distinct biochemical alterations the genetically determined hypertension in rats could not be influenced by a long-lasting diet containing a high amount of LA which has been proved to be effective on lowering blood pressure in other hypertensive rat models.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 2-3","pages":"215-25"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18129833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Diet induced changes of Ca-regulation in rabbit papillary muscle. 1. Contraction and relaxation following a cholesterol diet]. 饮食对兔乳头肌钙调节的影响。1. 胆固醇饮食后的收缩和放松]。

Acta biologica et medica Germanica Pub Date : 1982-01-01

J Günther, E Kutscherskij, E Storch, R Vetter

引用次数: 0

[A new human Gm reaction type, tested on bovine serum]. [一种新的人类转基因反应类型，在牛血清上测试]。

Acta biologica et medica Germanica Pub Date : 1982-01-01

R Kirst

引用次数: 0

[Circadian rhythm of mortality, spontaneous locomotor activity, and LD50 following application of 1,1,2,2,-tetrabromoethane alone and in combination with ethanol]. [单独使用1,1,2,2，-四溴乙烷和与乙醇联合使用后死亡率、自发运动活动和LD50的昼夜节律]。

Acta biologica et medica Germanica Pub Date : 1982-01-01

D L Wolff

引用次数: 0

The effect of litter size on the development of the endocrine rat pancreas. 胎次对大鼠胰腺内分泌发育的影响。

Acta biologica et medica Germanica Pub Date : 1982-01-01

S Noack, W Besch, I Komolov, H J Hahn

引用次数: 0

Correlations between the presence of islet cell surface antibodies in human serum and the cytotoxic effects on rat islet beta-cells. 人血清中胰岛细胞表面抗体的存在与对大鼠胰岛β细胞的细胞毒性作用之间的关系。

Acta biologica et medica Germanica Pub Date : 1982-01-01

H Jahr, S Marx, W Besch, K P Ratzmann

引用次数: 0

Proteolytic activities associated with plasma membrane preparations from tumour cells in enzootic bovine leukosis and from normal bovine lymphoid cells. 牛地方性白血病肿瘤细胞和正常牛淋巴样细胞质膜制备的蛋白水解活性。

Acta biologica et medica Germanica Pub Date : 1982-01-01

F D Böhmer, H E Schmidt, R Schön

{"title":"Proteolytic activities associated with plasma membrane preparations from tumour cells in enzootic bovine leukosis and from normal bovine lymphoid cells.","authors":"F D Böhmer, H E Schmidt, R Schön","doi":"","DOIUrl":"","url":null,"abstract":"Proteolytic activities associated with plasma membrane preparations from cells of tumourous lymph nodes in enzootic bovine leukosis and from normal bovine lymphoid cells have been studied. 125I-Casein-Sepharose has been used as a substrate. No significant difference between both cell types has been found in total proteolytic activity at different pH-values as well as in the number and type of proteinases, the membranes seem to be equipped with. The main proteolytic activities present in both types of membranes are: One (or two) pepstatin-inhibitable aspartate-proteinase(s) with maximum activity at pH 3 and 5, a leupeptin-inhibitable, presumably cysteine-proteinase, maximally active at pH 4, very small activity(ies?) between pH 7 and 8 of uncertain nature, and a plasminogen-activator with maximum activity around pH 8. Autodigestion of plasma membranes from normal lymph node lymphocytes has been performed choosing conditions optimal for the different proteinases. The only specific proteolysis observed is the splitting of a glycoprotein (151.5 kD), which is present in the plasma membranes of normal lymph node lymphocytes but not in the plasma membranes of peripheral blood lymphocytes and tumour cells, under conditions optimal for the aspartate-proteinase. A possible role of this specific proteolysis is suggested for tumouri-genesis and/or loss of sessility of normal lymphocytes.","PeriodicalId":6985,"journal":{"name":"Acta biologica et medica Germanica","volume":"41 10","pages":"883-90"},"PeriodicalIF":0.0,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17949549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0