[Isolation of an alpha-globulin from rabbit serum responsible for a cobalt(II) ion-induced change in conformation of fibrinogen].

Abstract

A change in conformation of fibrinogen, caused by cobaltous ions after parenteral application or incubation of plasma, is dependent on the presence of species specific plasma proteins. These proteins, which can be found only in some animals, have not yet been identified. An glycoprotein, responsible for the cobalt effect, was isolated from rabbit serum. It migrates immunoelectrophoretically as an alpha-globulin. In the SDS electrophoresis it was shown to migrate in front of albumin. The actions of this protein, induced by cobaltous ions, could be inhibited by complexing and SH groups alkylating compounds.