Low dose streptozotocin diabetes after partial pancreatectomy in dogs. Histological findings in a new type of experimental diabetes.

Acta biologica et medica Germanica Pub Date : 1982-01-01
E J Freyse, H Hahn von Dorsche, U Fischer
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Abstract

Permanent diabetes was produced in 16 out of 55 dogs by partial pancreatectomy (77% of the calculated organ weight) and simultaneous infusion of 2 mg/kg streptozotocin into the superior pancreaticoduodenal artery. The animals exhibited hyperglycemia, absolute lack of endogenous B-cell function, and ketosis, but no exocrine pancreatic insufficiency. 21 animals needed up to 7 additional subsequent intravenous streptozotocin injections (15 mg/kg each at intervals of 3 days). In 18 animals the procedure failed to render them diabetic; they died mainly from toxic effects of the drug. There were severe pathohistological changes in all streptozotocin-treated animals. Besides the well known alterations of the islets of Langerhans, lymphocytic inflammations were found in numerous organs including the exocrine pancreas. In most cases they were combined with degenerative changes of the organ parenchyma, particularly in kidney and liver. These findings were not correlated to the sex of the animals, to the occurrence and severity if diabetes, to the time of survival, or to the streptozotocin dose applied. But they were obviously related to the clinical picture existing besides diabetes. It is concluded that the model of experimental diabetes presented might be useful in a carnivorous big animal species but that toxic streptozotocin effects are to be expected when the dose administered exceeds 2 mg/kg.

狗部分胰腺切除术后低剂量链脲佐菌素糖尿病。一种新型实验性糖尿病的组织学发现。
55只狗中有16只通过部分胰腺切除术(占计算器官重量的77%)并同时向胰十二指肠上动脉注入2 mg/kg链脲佐菌素产生永久性糖尿病。这些动物表现出高血糖、内源性b细胞功能绝对缺乏和酮症,但没有外分泌胰腺功能不全。21只动物随后需要7次静脉注射链脲佐菌素(每次15 mg/kg,间隔3天)。在18只动物中,手术未能使它们患上糖尿病;他们主要死于药物的毒性作用。所有链脲佐菌素治疗的动物都有严重的病理组织学改变。除了众所周知的朗格汉斯胰岛的改变外,在包括外分泌胰腺在内的许多器官中都发现了淋巴细胞炎症。在大多数情况下,它们合并了器官实质的退行性改变,特别是在肾脏和肝脏。这些发现与动物的性别、糖尿病的发生和严重程度、生存时间或应用链脲佐菌素的剂量无关。但它们明显与糖尿病以外存在的临床症状有关。由此得出结论,所建立的实验性糖尿病模型可能适用于大型食肉动物,但当给药剂量超过2 mg/kg时,将产生毒性链脲佐菌素效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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