Acta Botanica Croatica最新文献_第8页

Colonization of bacteria and diatoms on an artificial substrate in a marine lake (eastern Adriatic Sea, NE Mediterranean) 细菌和硅藻在海洋湖泊（亚得里亚海东部，地中海东北部）人工基质上的定殖

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-028

A. Car, D. Hafner, S. Ljubimir, I. D. Radić, Svjetlana Bobanović-Ćolić, N. Jasprica

{"title":"Colonization of bacteria and diatoms on an artificial substrate in a marine lake (eastern Adriatic Sea, NE Mediterranean)","authors":"A. Car, D. Hafner, S. Ljubimir, I. D. Radić, Svjetlana Bobanović-Ćolić, N. Jasprica","doi":"10.37427/botcro-2020-028","DOIUrl":"https://doi.org/10.37427/botcro-2020-028","url":null,"abstract":"The initial colonization of bacteria and diatoms on a immersed artificial substrate and the development of diatom assemblages in relation to physico-chemical parameters were investigated on a weekly basis at one station in the marine Lake Mrtvo More, South Croatia, from April to October 2016. According to TRIX trophic index, lake showed different trophic character: (i) oligotrophic (at the beginning and the end of the study), (ii) mesotrophic (the end of June to mid-July), (iii) eutrophic (the end of July to mid-September). Heterotrophic bacteria increased to peak abundances (69,268 cells cm–2) at the beginning of June when the diatoms abundances start to increase. The lake has high diatom species richness (285 diatom taxa within 72 genera), with the highest species diversity index in August. Among diatoms, adnate were the primary colonizers, particularly Cocconeis dirupta W.Gregory var. flexella (Janisch and Rabenhorst) Grunow and Cocconeis scutellum Ehrenberg var. scutellum, while motile taxa joined the fouling communities from July to September. This study showed close relationship between diatom species composition and changes of physico-chemical parameters, particularly the nutrient concentrations.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"79 1","pages":"212-227"},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46418252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Sternbergia colchiciflora Waldst. et Kit. (Amaryllidaceae), a new genus and species in the flora of Kosovo 斯特恩伯格（Sternbergia colchiciflora Waldst）。et套件。（石蒜科），科索沃植物区系中的一个新属和新种

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-030

Xhavit Mala, Qenan Maxhuni

引用次数: 0

Effects of TiO2 nanoparticles and spermine on antioxidant responses of Glycyrrhiza glabra L. to cold stress 纳米TiO2和精胺对光甘草抗氧化反应的影响

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-025

Vahideh Kardavan Ghabel, R. Karamian

{"title":"Effects of TiO2 nanoparticles and spermine on antioxidant responses of Glycyrrhiza glabra L. to cold stress","authors":"Vahideh Kardavan Ghabel, R. Karamian","doi":"10.37427/botcro-2020-025","DOIUrl":"https://doi.org/10.37427/botcro-2020-025","url":null,"abstract":"Licorice (Glycyrrhiza glabra L.) is known as an important medicinal plant throughout the world. Glycyrrhizin is one of the most important specialized metabolites produced by licorice. In order to study the effect of TiO2 nanoparticles (TiO2 NPs) and spermine on physiological and biochemical traits of licorice under cold stress conditions, a factorial experiment was conducted in a completely randomized design with three replications. Plants were exposed to optimum temperature (26 oC) as control and low temperature (4 oC) as cold stress conditions and also treated with TiO2 NPs (2 and 5 ppm) and spermine (1 mM), separately. Results from physiological and biochemical analyses of the aerial parts of licorice seedlings showed that the growth parameters and the content of photosynthetic pigments decreased in response to low temperature. TiO2 NPs and spermine treatments increased plant resistance to cold stress and decreased the level of oxidative damage by reduction of malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents. In other hand, TiO2 NPs and spermine caused increase of phenolics, total protein and osmolytes contents under cold stress conditions. An increase in glycyrrhizin content was significantly induced by low temperature, TiO2 NPs and spermine.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"79 1","pages":"137-147"},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49361795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Influence of dehydration on cryopreservation of Musa spp. germplasm 脱水对穆萨种质冷冻保存的影响

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-024

E. Kaya, F. V. Souza, J. Santos-Serejo, S. Galatali

{"title":"Influence of dehydration on cryopreservation of Musa spp. germplasm","authors":"E. Kaya, F. V. Souza, J. Santos-Serejo, S. Galatali","doi":"10.37427/botcro-2020-024","DOIUrl":"https://doi.org/10.37427/botcro-2020-024","url":null,"abstract":"Cryopreservation is an important technique for the long-term storage of economically important plant germplasm. In this study, an efficient protocol was developed for the long-term conservation of seven economically important Musa taxa: M. acuminata Colla ssp. burmannica N.W. Simmonds, M. acuminata Colla ssp. zebrina (Van Houtte) R.E. Nasution, M. balbisiana Colla, M. basjoo Sieb., M. ornata W. Roxburgh (St. Lavender), M. velutina H. Wendl. et Drude (Velvet Pink Banana), and M. acuminata × balbisiana. The seeds were dehydrated in a sterile laminar flow cabinet for different exposure times and then they were directly immersed in liquid nitrogen. The critical point was to support the initial germination of cryopreserved seeds and this was achieved by the excision of zygotic embryos after liquid nitrogen treatment that allowed the seed germination. The best moisture content for tolerance to cryopreservation ranged from 15.8% (M. acuminata ssp. zebrina) to 17.1% (M. ornata) and the maximum post-cryopreservation germination rates varied from 86.4% (M. velutina) to 55.0% (M. ornata). All seedlings derived from seeds germinated after cryopreservation were easily rooted and acclimated to greenhouse conditions.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"79 1","pages":"99-104"},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44537674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Biosynthesis and characterization of iron nanoparticles produced by Thymus vulgaris L. and their antimicrobial activity 寻常胸腺制备铁纳米颗粒的生物合成、表征及其抑菌活性

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-032

R. Yehia, Ali M. Ali

{"title":"Biosynthesis and characterization of iron nanoparticles produced by Thymus vulgaris L. and their antimicrobial activity","authors":"R. Yehia, Ali M. Ali","doi":"10.37427/botcro-2020-032","DOIUrl":"https://doi.org/10.37427/botcro-2020-032","url":null,"abstract":"Research in the area of nanoparticles has grown considerably in recent years. Plant leaf extracts provide a platform for nanoparticle synthesis from metal and metal oxides, which is more economical and environmentally friendly than other methods, such as chemical reduction and physical methods. The present study conducted the biosynthesis of iron nanoparticles (FeNPs) using Thymus vulgaris L. (Thyme) leaf aqueous extract. The characterization of FeNPs was carried out by transmission electron microscopy (TEM), UV-visible spectrophotometry (UV-VIS), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-Ray diffraction (XRD) techniques. UV-VIS spectroscopy analysis demonstrated a visible peak around 440 nm. FTIR demonstrated the presence of iron metallic ions. Structural analysis of the nanoparticles by TEM showed agglomerations of spherical shapes. The average size of the synthesized FeNPs was around 40 nm. Regarding application, the ability of the FeNPs to degrade methyl orange was recorded as 95%. They were also examined for potential antimicrobial activity against pathogenic Gram-positive and Gram-negative bacteria and fungi. FeNPs demonstrated high antifungal activity against Candida albicans, C. parapsilosis and Aspergillus flavus, while their antibacterial activity was much weaker compared to commercial antibacterial agent. Thus, FeNPs synthesized using T. vulgaris could play an important role in controlling C. albicans, C. parasilosis and A. flavus and bioremediation of dyes.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"79 1","pages":"114-120"},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48420316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Astragalus bartinense (Fabaceae), a new species from Turkey 标题土耳其黄芪(豆科)一新种

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-023

B. Tunçkol, Ahter Fişne, N. Aksoy, Z. Aytaç

引用次数: 6

De novo transcriptome analysis of high growth rate Pyropia yezoensis (Bangiales, Rhodophyta) mutant with high utilization of nitrogen 高氮利用率高生长yezoensis (Bangiales, Rhodophyta)突变体从头转录组分析

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-026

SeoHwa Park, Jong-il Choi

{"title":"De novo transcriptome analysis of high growth rate Pyropia yezoensis (Bangiales, Rhodophyta) mutant with high utilization of nitrogen","authors":"SeoHwa Park, Jong-il Choi","doi":"10.37427/botcro-2020-026","DOIUrl":"https://doi.org/10.37427/botcro-2020-026","url":null,"abstract":"Pyropia yezoensis (Ueda) M.S.Hwang et H.G.Choi (Bangiales, Rhodophyta) has potentially high economic value. P. yezoensis has been used as food in East Asian countries for a long time, and, in addition to that its consumption is increasing worldwide owing to the growing interest in healthy seaweed food. A mutant (Py2K) with a high growth rate was developed using gamma rays to increase the production of P. yezoensis. De novo transcriptome analysis was performed to determine the mechanism underlying the high growth rate of this mutant. The transcriptomes from wild-type (PyWT) and mutant (Py2K) strains were assembled, and 167,165 genes were analyzed. A total of 15,979 genes were differentially expressed. Transcriptome analysis of nitrogen pathway revealed the increase in nitrogen availability through the upregulation of nitrate transporter gene (nrt) expression. Activation of nitrogen assimilation and re-assimilation and upregulation in alternative oxidase (aox) gene contributed to the increase in cellular nitrogen availability, thereby affecting the synthesis of phycobiliprotein. As a consequence, the efficiency of photosynthesis and the subsequent growth rate increased, which contributed to the color differences in thalli between PyWT and Py2K.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"79 1","pages":"201-211"},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41690910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Comparative transcriptome analysis of high-growth and wild-type strains of Pyropia yezoensis 野鱼Pyropia高生长株与野生株的比较转录组分析

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-020

Kim Ngan Tran, Jong-il Choi

{"title":"Comparative transcriptome analysis of high-growth and wild-type strains of Pyropia yezoensis","authors":"Kim Ngan Tran, Jong-il Choi","doi":"10.37427/botcro-2020-020","DOIUrl":"https://doi.org/10.37427/botcro-2020-020","url":null,"abstract":"Pyropia yezoensis (Ueda) M.S.Hwang et H.G.Choi is a popular edible macro-alga that is found mostly in intertidal zones. It is one of the most economically important seaweed species and has been cultivated extensively in the cold waters of East Asia. Various reports have been published on the isolation and characterization of improved strains of Pyropia. However, there are few studies focusing on the molecular basis underlying these mutant strains. In this study, we performed a comparative analysis of whole transcriptomes of wild-type (PyWT) and high-growth (Py500G) strains of P. yezoensis using next generation RNA sequencing (RNA-seq). After sequencing, a total of 167,110,896 paired-end reads with a length of 151 nucleotides, were obtained. De novo transcriptome assembly and redundancy removal generated 19,441 transcripts. The assembly was annotated in NCBI nr, Swiss-Prot, Pfam, KEGG, GO and KOG databases. To unravel the differences in Py500G and PyWT, we mapped Py500G and PyWT reads to the assembly and calculated the expression levels. In total, there were 454 transcripts that were differentially expressed. Among the differentially expressed transcripts, candidate genes were identified with well-known growth and development functions. This study not only identifies candidate genes responsible for the high-growth phenotype of Py500G, but it also provides more comprehensive genomic data for future research on P. yezoensis.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"79 1","pages":"148-156"},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46184797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Metabarcoding Cyanobacteria in coastal waters and sediment in central and southern Adriatic Sea 亚得里亚海中部和南部沿海水域和沉积物中的代谢蓝藻

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-021

Anamarija Kolda, Z. Ljubešić, A. Gavrilovic, Jurica Jug-Dujakovic, K. Pikelj, D. Kapetanović

{"title":"Metabarcoding Cyanobacteria in coastal waters and sediment in central and southern Adriatic Sea","authors":"Anamarija Kolda, Z. Ljubešić, A. Gavrilovic, Jurica Jug-Dujakovic, K. Pikelj, D. Kapetanović","doi":"10.37427/botcro-2020-021","DOIUrl":"https://doi.org/10.37427/botcro-2020-021","url":null,"abstract":"Seasonal sampling of the seawater column and sediment in Adriatic coastal areas affected by various anthropogenic activities, primarily aquaculture, was conducted during 2017. In total, 32 samples from two sites (central and southern Adriatic) were analysed by 16S rRNA amplicon sequencing. This approach was selected to test the possibilities of using metabarcoding in studying marine cyanobacteria, exploring their ecology and potential as an indicator group in anthropologically stressed coastal environments. Additionally, physicochemical water column parameters, sediment granulometry and composition were assessed. Water column revealed a seasonal variation of amplicon sequencing variants (ASVs) closely related to Cyanobium PCC-6307, Prochlorococcus MIT9313 and Synechococcus CC9902, as well as seasonal grouping of physico-chemical parameters in PCA analysis. Sediment analysis uncovered greater community richness of 13 cyanobacterial genera and two uncultured groups. The most abundant in sandy gravels and gravelly sand type of sediments were ASVs closely related to Pleurocapsa PCC-7319 and Xenococcus PCC-7305. Furthermore, identified cyanobacterial ASVs predominantly displayed similarity to isolates from tropical areas (e.g. Neolyngbya, Chroococcidiopsis, Trichodesmium, etc.), which could indicate the tropicalization process already ongoing in the fish fauna of the Adriatic Sea.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46274090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Citruses in Croatia – cultivation, major virus and viroid threats and challenges 克罗地亚的柠檬——种植、主要病毒和类病毒威胁和挑战

IF 1.3 4区生物学

Acta Botanica Croatica Pub Date : 2020-10-01 DOI: 10.37427/botcro-2020-027

S. Černi, K. Hančević, D. Škorić

{"title":"Citruses in Croatia – cultivation, major virus and viroid threats and challenges","authors":"S. Černi, K. Hančević, D. Škorić","doi":"10.37427/botcro-2020-027","DOIUrl":"https://doi.org/10.37427/botcro-2020-027","url":null,"abstract":"Although Croatia is not often perceived as a citrus growing country, citrus species have been planted in the Croatian southern coastal part for centuries. Citrus had always been important as a source of vitamins and micronutrients for local consumption, but with the introduction of Satsuma mandarins (Citrus unshiu Marc.) citrus production started its commercial development. The Croatian coast is probably the northernmost commercial citrus growing area globally with several agroecological constraints influencing citrus production. However, Satsuma mandarins, the most cold-tolerant citrus of economic interest, are successfully cultivated resulting in an excellent quality of fruits that easily finds a market niche. Unfortunately, with the import of plant material in the last century, the simultaneous introduction of viral and subviral pathogens occurred. The most important are Citrus tristeza virus and Citrus exocortis viroid. As certain rootstock-scion combinations are tolerant to virus or viroid infections and display no obvious symptoms, these pathogens have been continuously spreading by plant propagation. Molecular and biological analyses revealed the existence of pathotypes not believed to be endemically present in the Mediterranean region. Their high intra-isolate genetic variability and frequent coinfections suggest that this geographical region presents a sort of a biological enclave of potentially threatening pathogens. Their transmission from this agroecological niche to areas where sensitive rootstock-scion combinations are common presents a serious risk to citrus production, especially when global warming and the resulting migration of efficient vector species are considered. Comprehensive pathogen monitoring is important for improving citrus production and the prevention of pathogen transmission.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"79 1","pages":"228-235"},"PeriodicalIF":1.3,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.37427/botcro-2020-027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49388461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2