Acta endocrinologica最新文献_第6页

Effects of long-term estradiol exposure on the hypothalamic neuron number. 长期雌二醇暴露对下丘脑神经元数量的影响。

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290543

S L Yang, C Hsu, H K Hsu, K M Liu, M T Peng

引用次数: 16

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290594

E V Bolshova, N D Tronko, L VanMiddlesworth

引用次数: 11

Efficacy of insulin-like growth factor binding protein 3 in predicting the growth hormone response to provocative testing in children treated with cranial irradiation. 胰岛素样生长因子结合蛋白3在预测儿童颅脑照射刺激试验中生长激素反应中的作用

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290511

C Sklar, K Sarafoglou, E Whittam

{"title":"Efficacy of insulin-like growth factor binding protein 3 in predicting the growth hormone response to provocative testing in children treated with cranial irradiation.","authors":"C Sklar, K Sarafoglou, E Whittam","doi":"10.1530/acta.0.1290511","DOIUrl":"https://doi.org/10.1530/acta.0.1290511","url":null,"abstract":"Recent data suggest that the plasma concentration of insulin-like growth factor binding protein 3 (IGFBP-3) is useful as a screening test for growth hormone (GH) deficiency. In this study, we measured by radioimmunoassay the levels of IGFBP-3 in a group of 20 subjects (12 males) of 5 years and 7 months to 16 years of age undergoing standard GH testing following cranial irradiation. The patients had received 1800 to > 6000 cGY of radiation to the hypothalamic-pituitary region, a median of 2.7 years (range 2-7 years) prior to testing. The IGFBP-3 concentrations were discordant with the results of GH testing 60% (12/20) of the time. Although IGFBP-3 levels were below the mean for age in 14 of 15 GH-deficient (peak GH < 10 micrograms/l) patients, only three of 15 GH-deficient patients had IGFBP-3 concentrations that fell below age-adjusted norms. In contrast, the IGFBP-3 levels were within the normal range in all five patients with normal GH responses. The low sensitivity (20%) of IGFBP-3 in predicting the subjects with abnormal responses was not improved by adjusting the values for bone age or stage of puberty. We concluded that a single plasma determination of IGFBP-3 is not a useful screening test for GH deficiency among patients previously treated with cranial irradiation.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 6","pages":"511-5"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290511","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18517258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 71

Localization of Cu/Zn and Mn superoxide dismutase in various thyroid disorders. 各种甲状腺疾病中铜锌锰超氧化物歧化酶的定位。

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290573

K Iwase, A Nagasaka, K Kato, S Ohtani, T Tsujimura, A Inagaki, S Jimbo, A Nakai, R Masunaga, M Hamada

{"title":"Localization of Cu/Zn and Mn superoxide dismutase in various thyroid disorders.","authors":"K Iwase, A Nagasaka, K Kato, S Ohtani, T Tsujimura, A Inagaki, S Jimbo, A Nakai, R Masunaga, M Hamada","doi":"10.1530/acta.0.1290573","DOIUrl":"https://doi.org/10.1530/acta.0.1290573","url":null,"abstract":"The intracellular localization of Cu/Zn- and Mn-superoxide dismutase (SOD), which catalyze the dismutation of superoxide radicals (O2-) to O2 and H2O2, was studied in the thyroid tissue of various thyroid disorders by an immunohistochemical technique. The concentrations of both SODs in those tissues were measured also by a sandwich enzyme immunoassay technique. Copper/zinc-SOD in thyroid tissues were identified by immunocytochemical staining in most cases of papillary carcinoma and in some cases of other thyroid disorders. In normal follicular cells this enzyme is localized in the perinuclear cytoplasm, whereas in thyroid tumor or hyperplastic follicular cells it exists homogeneously in cytoplasm. Manganese-SOD stained strongly in papillary carcinoma and papillary-growing cells in the thyroid tissue of adenoma and Graves' disease. The concentrations of Cu/Zn-and Mn-SOD in thyroid tumor tissues and hyperplastic follicular disorders were significantly higher than those in normal thyroid tissue when they were compared as a function of protein or deoxyribonucleic acid contents. The ratio of Mn-SOD to Cu/Zn-SOD was significantly higher only in papillary carcinoma, except for other thyroid disorders as compared with that in the normal thyroid. In conclusion, SOD seems to be related to cell proliferation and differentiation in the thyroid follicular cell because Cu/Zn-SOD changes its localization in tumor and hyperplastic follicular cells and because the Mn-SOD concentration is increased in papillary carcinoma or papillary-growing cells.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 6","pages":"573-8"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290573","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18517260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Iodine deficiency disorders. 缺碘症。

Acta endocrinologica Pub Date : 1993-12-01

H Bürgi

引用次数: 0

Iodized salt prophylaxis of endemic goiter: an experience in Toscana (Italy). 碘盐预防地方性甲状腺肿:托斯卡纳(意大利)的经验。

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290497

F Aghini-Lombardi, A Pinchera, L Antonangeli, T Rago, G F Fenzi, P Nanni, P Vitti

{"title":"Iodized salt prophylaxis of endemic goiter: an experience in Toscana (Italy).","authors":"F Aghini-Lombardi, A Pinchera, L Antonangeli, T Rago, G F Fenzi, P Nanni, P Vitti","doi":"10.1530/acta.0.1290497","DOIUrl":"https://doi.org/10.1530/acta.0.1290497","url":null,"abstract":"It is well established that iodine supplementation is effective in correcting iodine deficiency and reducing goiter prevalence. In Italy, legislation has allowed the production of iodized salt since 1972, but its consumption is on a voluntary basis. In the present study, the efficacy of legislative measures that made compulsory the availability of iodized salt in foodstores has been evaluated. Urinary iodine excretion and thyroid size, scored according to Pan American Health Organization recommendations, were determined prior to (1981) and 10 years after (1991) the introduction of legislative measures in the whole schoolchildren population residing in a restricted area of the Tuscan Appennines. Moreover, in 1991, thyroid volume was determined by ultrasonography. In 1981, mean urinary iodine excretion was 47.1 +/- 22.4 mg/kg creatinine (0.412 mumol/l) and goiter prevalence was 60%, indicating a moderate iodine deficiency. Eighty of the families subsequently used iodized salt on a regular basis; as a result of this excellent compliance, in 1991 the mean urinary iodine excretion increased to 129.7 +/- 73 mg/kg creatinine (1.24 mumol/l) and goiter prevalence dropped to 8.1%. The results of this study underline the effectiveness of iodine prophylaxis in correcting iodine deficiency and abating endemic goiter in schoolchildren, and suggest that implementation of measures that make compulsory the availability of iodized salt in foodstores overcomes the fact that there is no law governing the exclusive production and trading of iodized salt.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 6","pages":"497-500"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290497","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Production of alpha-subunit of glycoprotein hormones by pituitary somatotroph adenomas in vitro. 垂体生长发育腺瘤体外产生糖蛋白激素α亚基的研究。

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290565

G Kontogeorgos, S L Asa, K Kovacs, H S Smyth, W Singer

{"title":"Production of alpha-subunit of glycoprotein hormones by pituitary somatotroph adenomas in vitro.","authors":"G Kontogeorgos, S L Asa, K Kovacs, H S Smyth, W Singer","doi":"10.1530/acta.0.1290565","DOIUrl":"https://doi.org/10.1530/acta.0.1290565","url":null,"abstract":"Somatotroph adenomas of the pituitary secrete growth hormone in excess and are associated with acromegaly. Morphologically, they can be separated into two entities, densely and sparsely granulated variants. It has been shown that a number of somatotroph adenomas produce alpha-subunit of glycoprotein hormones; however, it is not clear whether alpha-subunit production correlates with tumor cell morphology. We studied 32 surgically removed pituitary somatotroph adenomas in tissue culture to determine structure-function correlations of growth hormone and alpha-subunit production. All tumors were classified on the basis of detailed histological, immunocytochemical and electron-microscopic studies. Fifteen tumors were densely granulated and 17 were sparsely granulated. In addition to growth hormone, all 15 densely granulated tumors released alpha-subunit in vitro, whereas of the 17 sparsely granulated tumors only 4 released alpha-subunit; moreover, the mean baseline levels of alpha-subunit were significantly higher in densely granulated adenomas than in sparsely granulated adenomas. Parallel response of release of both hormones was found during stimulation with growth hormone-releasing hormone or thyrotropin-releasing hormone and during suppression with somatostatin or bromocriptine in densely granulated tumors. alpha-subunit response to stimulation or suppression could not be determined with significance in sparsely granulated tumors because of low basal levels. The results indicate that alpha-subunit production and release is characteristic of densely granulated somatotroph adenomas and that alpha-subunit is coregulated with growth hormone by adenohypophysiotropic substances; in contrast, alpha-subunit production, by sparsely granulated somatotroph adenomas is rare and, when present, much lower in quantity.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 6","pages":"565-72"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18517259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

FK506 inhibits phytohemagglutinin-, but not interferon-gamma-, induced HLA-DR antigen expression and accessory cell function on primary cultured human thyroid cells. FK506对原代培养的人甲状腺细胞抑制植物血凝素诱导的HLA-DR抗原表达和辅助细胞功能，但不抑制干扰素诱导的HLA-DR抗原表达。

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290579

M Sato, Y Hiromatsu, K Tanaka, N Ishisaka, K Nonaka

{"title":"FK506 inhibits phytohemagglutinin-, but not interferon-gamma-, induced HLA-DR antigen expression and accessory cell function on primary cultured human thyroid cells.","authors":"M Sato, Y Hiromatsu, K Tanaka, N Ishisaka, K Nonaka","doi":"10.1530/acta.0.1290579","DOIUrl":"https://doi.org/10.1530/acta.0.1290579","url":null,"abstract":"We investigated the effects of FK506, a novel immunosuppressive agent, on the phytohemagglutinin (PHA) or interferon-gamma (IFN-gamma)-induced expression of HLA-DR antigen, accessory cell function and proliferation of primary cultured human thyroid cells. Primary cultured thyroid cells from patients with Graves' disease were incubated for 3 days with PHA in concentrations in the range 1-50 mg/l or with 200 kU/l of IFN-gamma, in the presence or absence of FK506. The surface expression of HLA-DR antigen was measured by flow cytometry. Accessory cell function of thyroid cells was assessed by the incorporation of [3H]thymidine to T cells in the presence of 0.1-1.0 micrograms/l staphylococcus enterotoxin B (SEB). The proliferation of thyroid cells was determined from [3H]thymidine incorporation assays. FK506 inhibited the induction of HLA-DR antigen expression by PHA on thyroid cells in a dose-dependent manner, but did not inhibit that by IFN-gamma. Polyclonal anti-IFN-gamma antibody partly inhibited the PHA-induced HLA-DR antigen expression on thyroid cells. Phytohemagglutinin enhanced the SEB-mediated accessory cell function of thyroid cells. FK506 inhibited the accessory cell function induced by PHA. FK506 alone did not directly affect the thyroid cell proliferation, although it ameliorated the thyroid cell growth suppressed by PHA. Our data suggest that FK506 suppresses the HLA-DR antigen expression induced by PHA and the subsequent accessory cell function on thyroid cells via the inhibition of T lymphocytes present in the primary culture.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 6","pages":"579-84"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18517261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Induction of reversible growth retardation and growth hormone deficiency by blockade of norepinephrine synthesis in the rat. 阻断去甲肾上腺素合成诱导大鼠可逆性生长迟缓和生长激素缺乏。

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290554

S Malozowski, E Mamalaki, M Pleti, I Armando, D Goldstein, G R Merriam

{"title":"Induction of reversible growth retardation and growth hormone deficiency by blockade of norepinephrine synthesis in the rat.","authors":"S Malozowski, E Mamalaki, M Pleti, I Armando, D Goldstein, G R Merriam","doi":"10.1530/acta.0.1290554","DOIUrl":"https://doi.org/10.1530/acta.0.1290554","url":null,"abstract":"Norepinephrine is a major regulator of the release of growth hormone. Diethyldithiocarbamate, a dopamine-beta-hydroxylase inhibitor, reduces norepinephrine synthesis and acutely inhibits growth hormone (GH) secretion. To investigate the long-term effects of dopamine-beta-hydroxylase blockade on growth, we administered diethyldithiocarbamate (0, 40, 100 or 400 mg/kg sc b.i.d.) to 21-day-old female rats for 10 days. Food intake, body weight, and tail length were measured twice a week. Plasma GH levels and hypothalamic dopamine and norepinephrine content were measured; messenger ribonucleic acids (mRNAs) for GH-releasing hormone and somatostatin were determined by quantitative in situ hybridization. Diethyldithiocarbamate administration decreased GH levels (p < 0.05) and retarded growth in a dose-dependent manner (p < 0.05), without altering food intake. Co-administration of GH partially reversed the growth retardation in diethyldithiocarbamate-treated animals (p < 0.05). Diethyldithiocarbamate treatment also increased the hypothalamic dopamine/norepinephrine ratio (1.13 vs 0.41 control, p < 0.05). Local levels of GH-releasing hormone and somatostatin mRNA were not altered by treatment. After discontinuation of diethyldithiocarbamate, growth rates returned to normal or transiently even to supranormal values. Norepinephrine synthesis blockade with diethyldithiocarbamate provides a model for reversible growth retardation, in which GH levels are decreased in the absence of decreased GH-releasing hormone mRNA. These results support a role for norepinephrine in the regulation of normal growth.","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 6","pages":"554-8"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290554","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18904247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vasopressin as a neuroendocrine regulator of anterior pituitary hormone secretion. 加压素作为垂体前叶激素分泌的神经内分泌调节剂。

Acta endocrinologica Pub Date : 1993-12-01 DOI: 10.1530/acta.0.1290489

A Kjaer

{"title":"Vasopressin as a neuroendocrine regulator of anterior pituitary hormone secretion.","authors":"A Kjaer","doi":"10.1530/acta.0.1290489","DOIUrl":"https://doi.org/10.1530/acta.0.1290489","url":null,"abstract":"Secretion of the anterior pituitary hormones adrenocorticotropin (ACTH), beta-endorphin and prolactin (PRL) is complex and involves a variety of factors. This review focuses on the involvement of arginine-vasopressin (AVP) in neuroendocrine regulation of these anterior pituitary hormones with special reference to receptor involvement, mode of action and origin of AVP. Arginine-vasopressin may act via at least two types of receptors: V1- and V2-receptors, where the pituitary V1-receptor is designated V1b. The mode of action of AVP may be mediating, i.e. anterior pituitary hormone secretion is transmitted via release of AVP, or the mode of action may be permissive, i.e. the presence of AVP at a low and constant level is required for anterior pituitary hormones to be stimulated. Under in vivo conditions, the AVP-induced release of ACTH and beta-endorphin is mainly mediated via activation of hypothalamic V1-receptors, which subsequently leads to the release of corticotropin-releasing hormone. Under in vitro conditions, the AVP-stimulated release of ACTH and beta-endorphin is mediated via pituitary V1b-receptors. The mode of action of AVP in the ACTH and beta-endorphin response to stress and to histamine, which is involved in stress-induced secretion of anterior pituitary hormones, is mediating (utilizing V1-receptors) as well as permissive (utilizing mainly V1-but also V2-receptors). The AVP-induced release of PRL under in vivo conditions is conveyed mainly via activation of V1-receptors but V2-receptors and probably additional receptor(s) may also play a role.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":6910,"journal":{"name":"Acta endocrinologica","volume":"129 6","pages":"489-96"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1530/acta.0.1290489","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19100763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47