Acta anatomicaPub Date : 1997-07-01DOI: 10.1159/000147965
Yakup Gumusalan, A. Kalaycıoğlu, Fatih Yazar, Yasin Arifoglu, Ahmet Sinav
{"title":"Accessory extensor carpi radialis muscle and interconnecting muscular bundle.","authors":"Yakup Gumusalan, A. Kalaycıoğlu, Fatih Yazar, Yasin Arifoglu, Ahmet Sinav","doi":"10.1159/000147965","DOIUrl":"https://doi.org/10.1159/000147965","url":null,"abstract":"An accessory muscle and a muscular bundle were found and prepared in both forearms of a 55-year-old male cadaver. On the left side, the accessory muscle-originated from the medial aspect of the extensor carpi radialis brevis (ECRB) muscle, coursed downwards, crossed posterior to the tendon of ECRB, passed through the second chamber of the extensor retinaculum and inserted into the base of second metacarpal bone. Additionally, a muscular bundle was observed between the extensor carpi radialis longus (ECRL) and ECRB muscles. On the right side, both ECRL and ECRB had bifid tendons. The long and thin additional tendon of ECRL coursed downwards and joined the accessory tendon of the ECRB before entering the second chamber of the extensor retinaculum and the common tendon inserted into the base of the second metacarpal bone.","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"32 1","pages":"57-60"},"PeriodicalIF":0.0,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78839788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000147958
M E Matthiessen, C Garbarsch, B Engelbrecht Olsen, S Hellström, A Engström-Laurent
{"title":"Hyaluronan in human deciduous tooth germs in the bell stage. Histochemistry and immunohistochemistry.","authors":"M E Matthiessen, C Garbarsch, B Engelbrecht Olsen, S Hellström, A Engström-Laurent","doi":"10.1159/000147958","DOIUrl":"https://doi.org/10.1159/000147958","url":null,"abstract":"<p><p>The primary aim of the present study was a localization of hyaluronan (HA) in human deciduous tooth germs in the bell stage. HA was compared to the content of chondroitin sulfates (CSs). HA was detected with a biotin-labeled HA-binding protein (HABP) and CS with a monoclonal antibody. As controls, enzyme digestions were carried out. Furthermore, the glycosaminoglycans were investigated histochemically with enzyme digestions followed by alcian blue staining. The investigation showed a considerable content of HA in the stellate reticulum, although CS was also found, primarily when treatment with protease was omitted. The dental papilla contained both HA and CS, while the predentin and the dentin contained only CS. The enamel did not contain any CS, but some staining with HABP was observed along the borderline between the ameloblasts and the enamel. The significance of HA in the stellate reticulum is discussed. The importance of carrying out investigations with and without protease digestions is stressed.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"159 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000147958","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20445893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000148016
H Yamamoto, J Sasaki, J Matsuura, T Nomura, E F Sato, H Shimokawa, K Ishizeki, T Nawa
{"title":"Characterization of the inner enamel epithelium in the enamel-free area based on the ability to secrete enamel protein demonstrated by in situ hybridization and immunohistochemistry.","authors":"H Yamamoto, J Sasaki, J Matsuura, T Nomura, E F Sato, H Shimokawa, K Ishizeki, T Nawa","doi":"10.1159/000148016","DOIUrl":"https://doi.org/10.1159/000148016","url":null,"abstract":"<p><p>Both the expression of amelogenin mRNA and secretion of amelogenin were investigated in rat molars by in situ hybridization and immunohistochemistry. Probes were designed by multiple-labeling of oligonucleotide probes for in situ hybridization. Amelogenin mRNA first appeared in differentiating ameloblasts of the distal region and some inner enamel epithelial cells of enamel-free area (EFA cells) of the second cusp at postnatal day 0. At the same time, amelogenin protein was detected in the extracellular matrix between dentin and differentiating ameloblasts and in some EFA cells of the second cusp. At postnatal day 1-3, amelogenin was expressed in the secretory ameloblasts, and in the matrix beneath these cells. Both amelogenin mRNA and amelogenin were detected in the EFA cells and their extracellular matrix. After postnatal day 5, amelogenin mRNA and amelogenin were detected in the secretory ameoloblasts and extracellular matrix in the enamel-forming region, respectively. At this time, amelogenin mRNA was not detected in the EFA cells, but a small amount of amelogenin was found in the matrix beneath the EFA cells. These findings suggest that EFA cells differentiate into amelogenin-secreting cells, i.e. ameloblasts, but that the secretion lasts for only a short period at the early stage of tooth development.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"160 4","pages":"232-8"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000148016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20645776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000147932
M Cellerini, A Konze, G Caracchini, M Santoni, G Dal Pozzo
{"title":"Magnetic resonance imaging of cerebral associative white matter bundles employing fast-scan techniques.","authors":"M Cellerini, A Konze, G Caracchini, M Santoni, G Dal Pozzo","doi":"10.1159/000147932","DOIUrl":"https://doi.org/10.1159/000147932","url":null,"abstract":"<p><p>Rapid scan techniques have introduced new sequence parameters as well as novel contrast concepts into everyday magnetic resonance imaging (MRI). In particular contrast characteristics of fast-spin echo (FSE) sequences showed some significant differences when compared to conventional spin echo images. The purpose of this work was to demonstrate the capabilities of FSE MRI in identifying and characterizing the in vivo anatomy of the main cerebral associative systems. Between March and November 1995, 20 healthy adult volunteers (12 males, 8 females, mean age 35 years) were submitted to a cranial MRI examination (1.5 Philips Gyroscan NT). In all cases axial and coronal 2-dimensional FSE T2-weighted and 2-dimensional inversion recovery FSE T1-weighted images were obtained. All MRI images were examined by a neuroradiologist (G. Dal Pozzo) for the depiction of the following compact white matter fiber bundles: anterior commissure, corpus callosum, superior fronto-occipital fasciculus, cingulum, fornix, mammillothalamic tract, uncinate fasciculus, superior and inferior longitudinal fasciculus. All these associative pathways could be well identified on T2-weighted images due to a lower signal intensity with respect to the surrounding white matter. On T1-weighted images only the corpus callosum, the anterior commissure and the fornix could always be identified. Correlation with myelin-specific colorations (Luxol fast blue stains) in anatomic atlases and a review of the literature on the myelinization process during infancy indicate that the short T2 relaxation times of the aforementioned cerebral associative systems may be due to heavy myelination and high fiber density. The correct visualization of interintrahemispheric associative white matter fiber bundles may play an important role in white matter disorders like dys- and demyelinating diseases and in the spreading of vasogenic edema and/or tumor being useful for their staging.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"158 3","pages":"215-21"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000147932","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20323703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000147977
G S Chleboun, T J Patel, R L Lieber
{"title":"Skeletal muscle architecture and fiber-type distribution with the multiple bellies of the mouse extensor digitorum longus muscle.","authors":"G S Chleboun, T J Patel, R L Lieber","doi":"10.1159/000147977","DOIUrl":"https://doi.org/10.1159/000147977","url":null,"abstract":"<p><p>The purpose of this study was to describe the extent to which architectural and fiber-type characteristics of the four bellies of the mouse extensor digitorum longus (EDL) suggest specialization of the digits, and to mathematically model the functional effects of the structural properties. Six mice were perfused in situ with glutaraldehyde while the lower limb was positioned approximately in the neutral position. After perfusion, lower limbs were removed and placed in glutaraldehyde until the EDL was dissected from the limb and separated into individual muscle bellies corresponding to each digit for architectural determination. The results showed that the muscle belly of digit 5 tended to be different from the muscle bellies of digits 2-4 for many architectural characteristics. Muscle mass, physiological cross-sectional area, muscle length, and fiber length were all significantly greater in digit 5. Proximal tendon length was also significantly longer in digit 5, and distal tendon length, as well as total tendon length, were significantly shorter in digit 5. Sarcomere length was shortest at the proximal end of the muscle and longest, 60-80%, toward the distal end. Fiber type distribution was about 60% FOG, 39% FG with only 1% SO fibers in all muscle bellies. Muscle-tendon modeling illustrated that peak force and maximal shortening velocity were greatest in digit 5. Inclusion of the tendon in the model resulted in a 10% shift of the force-length curve to longer lengths. Assuming muscle structure is matched to function, we speculate that digit 5 of the mouse EDL bears higher loads over a greater excursion during locomotion compared to the remaining digits.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"159 2-3","pages":"147-55"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000147977","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20495960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000147971
Y Ohira, W Yasui, R R Roy, V R Edgerton
{"title":"Effects of muscle length on the response to unloading.","authors":"Y Ohira, W Yasui, R R Roy, V R Edgerton","doi":"10.1159/000147971","DOIUrl":"https://doi.org/10.1159/000147971","url":null,"abstract":"<p><p>Muscle mass, distribution of fiber types, fiber cross-sectional areas (CSA) and selected enzyme activities were determined in rats hindlimb-suspended free of immobilization (Susp-Free), suspended with the ankle dorsiflexed (Susp-DF, soleus stretched) or plantarflexed (Susp-PF, soleus shortened) for 10 days and compared to cage-control (Con) rats. Reduction of muscle weight associated with suspension was prevented in Susp-DF rats. The mean CSAs of slow fibers were Con = Susp-DF > Susp > PF > Susp-Free and of fast and intermediate fiber tended to be Susp-DF > Con > Susp-PF = Susp-Free. Mean activities of succinate dehydrogenase (SDH), alpha-glycerophosphate dehydrogenase (GPD) and myofibrillar adenosine triphosphatase (mATPase) in slow and fast fibers were similar in Con and Susp-Free rats. Mean SDH activity in slow fibers was higher in Susp-DF and Susp-PF than in Con and Susp-Free. No significant differences in SDH activities of fast fibers were observed among groups. GPD activity was higher in slow fibers of Susp-DF and Susp-PF compared to Con. The mATPase activity was higher in slow fibers of Susp-DF compared to Con and Susp-Free rats and lower in fast fibers of Susp-DF compared to Con rats. Thus, when compared to control, the patterns of adaptation were more similar in the Susp-DF and Susp-PF than in the Susp-Free. Although these results are consistent with previous studies demonstrating that the load placed on a muscle can affect protein metabolism, the direction and magnitude of the adaptive responses observed in the present study were closely associated with the chronically imposed changes in muscle length, i.e. fixed at either a shortened or a lengthened position.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"159 2-3","pages":"90-8"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000147971","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20496100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000148002
J N Leijnse
{"title":"A generic morphological model of a muscle group--application to the muscles of the forearm.","authors":"J N Leijnse","doi":"10.1159/000148002","DOIUrl":"https://doi.org/10.1159/000148002","url":null,"abstract":"<p><p>In the present paper some aspects of the morphology of muscles in muscle groups are investigated modelwise. The analysis is initiated by the observation that the skeletal surfaces of the body are too small to provide the origin area for all muscles, and that therefore they must be enlarged by aponeuroses of muscular origin. These aponeuroses provide flexible surfaces of origin which allow to adapt the individual muscle shape to the various spaces available in the body, and to optimize their configuration in muscle groups. The shape of these origin surfaces determines the shape of the muscles and of their end tendons; this relationship is closely investigated. The models are illustrated by dissection examples of the muscle groups at the elbow,and may find an application in the teaching of clinical anatomy.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"160 2","pages":"100-11"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000148002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20591919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000148003
J N Leijnse, E T Walbeehm, G J Sonneveld, S E Hovius, J M Kauer
{"title":"Connections between the tendons of the musculus flexor digitorum profundus involving the synovial sheaths in the carpal tunnel.","authors":"J N Leijnse, E T Walbeehm, G J Sonneveld, S E Hovius, J M Kauer","doi":"10.1159/000148003","DOIUrl":"https://doi.org/10.1159/000148003","url":null,"abstract":"<p><p>In the carpal tunnel anatomical interconnections between the tendons of the musculus flexor digitorum profundus are systematically present. These interconnections limit the mutual tendon displacements, which decreases finger independence and may be problematic in a musician's hand. The present study investigates a possible role of the synovial sheaths in the formation of these intertendinous connections in the carpal tunnel. To this end a morphological model is provided which correlates the often distinctly fibrous structure of the deep flexor tendons in the carpal tunnel and the frequent exchange of tendon fibres between the tendons to the different fingers, with the tendency of the synovial membranes to strongly adhere to the tendons. This model is validated by gross dissection results, and by cross sections of the flexor tendons in the carpal tunnel. In agreement with the model, the anatomic data show that the synovial membranes tend to invade and become trapped in tendons made up from individualised tendon strands, and also strongly adhere to the substantial amounts of tendon fibres which may be exchanged between the flexor tendons proximal to the lumbrical origins. These fibres and the synovial membranes may form a strong fabric able to withstand substantial stretching forces of interconnected oppositely pulled flexor tendons.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"160 2","pages":"112-22"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000148003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20591920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000148000
W. Barańska, W. Baran, P. Skopiński, H. Ziemba
{"title":"Morphometric analysis of satellite cells in rat skeletal muscles: soleus and extensor digitorum longus.","authors":"W. Barańska, W. Baran, P. Skopiński, H. Ziemba","doi":"10.1159/000148000","DOIUrl":"https://doi.org/10.1159/000148000","url":null,"abstract":"Satellite cells (SC) are present in all types of skeletal muscles. They can exhibit DNA replication and mitotic divisions throughout their life. Depending on the kind of muscle, a different number of SC is present. The aim of this study was to establish whether the differences in muscle metabolism are reflected in the quantitative ultrastructural evaluation of their SC. Two skeletal muscles of rats, soleus (SOL) with predominant slow fibers and extensor digitorum longus (EDL) with predominant fast ones, were studied. Morphometric analysis was performed according to the modified method of Weibel [Stereological Methods, vol 1: Practical Methods for Biological Morphometry. London, Academic Press, 1979]. In the SC of EDL mitochondria, rough endoplasmic reticulum and Golgi apparatus were less frequent as compared with the SOL. The volume fractions of these organelles were much higher in SC of SOL than in EDL. The ribosomes and polyribosomes were more densely packed in SC of SOL than in EDL. Ultrastructural morphometric evaluation demonstrated significant differences between the SC components of two different muscles: a slow (SOL) and a fast (EDL) one.","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"5 1","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87437977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acta anatomicaPub Date : 1997-01-01DOI: 10.1159/000147986
S Vidal, A Román, L Moya
{"title":"Description of two types of mammosomatotropes in mink (Mustela vison) adenohypophysis: changes in the population of mammosomatotropes under different physiological conditions.","authors":"S Vidal, A Román, L Moya","doi":"10.1159/000147986","DOIUrl":"https://doi.org/10.1159/000147986","url":null,"abstract":"<p><p>The present study was undertaken to clarify the existence of mammosomatotropes (MS cells) in the mink adenohypophysis and their possible involvement in the interconversion of mammotrope and somatotrope cells under different physiological conditions: prepubertal, pubertal and adulthood. Electron microscope immunocytochemistry was used to detect growth hormone (GH) and prolactin (PRL) immunoreactivities in the anterior pituitary gland of mink. Primary antisera raised in rabbit (human anti-GH, NIDDK-AFP-1613102481; human anti-PRL, NIDDK-AFP-55781789) were localized with appropriate species-specific antisera coupled to colloidal gold particles of different sizes. MS cells were most frequently observed in adult mink. Double labeling for GH and PRL was presented in two types of MS cells. MS1 cells, observed only in adults, showed an irregular morphology, with many cytoplasmic processes. Within their cytoplasm there were numerous rounded secretory granules of approximately 135 nm mean diameter. In MS1 cells three types of secretory granules were identified. The most numerous contained only PRL. The least frequent contained only GH and the third type contained GH and PRL and appeared in an intermediate quantity. MS2 cells, observed in all age groups, presented a rounded morphology. These cells had a voluminous cytoplasm which showed little development of the organelles and contained a large number of rounded and very electron-dense secretory granules which measured about 160 nm mean diameter. Within MS2 cells two types of secretory granules, according to their immunolabeling, were observed. Monohormonal granules containing GH were the more abundant while bihormonal granules, containing GH and PRL, were present to a lesser degree. Data presented in this report suggest that MS cells could play a role in the interconversion of somatotropes into mammotropes in mink.</p>","PeriodicalId":6885,"journal":{"name":"Acta anatomica","volume":"159 4","pages":"209-17"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000147986","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20525777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}