Journal of the American Society for Mass Spectrometry最新文献_第10页

IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-06

Michael R. Hoopmann*, Christopher D. McGann, Christopher M. Rose and Devin K. Schweppe*,

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IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-06

Maggie Tam, and , Francisco Fernandez-Lima*,

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IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-06

Ting Zeng, Xueyun Zheng, Kevin Zemaitis, Dušan Veličković* and Christopher R. Anderton*,

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IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-06

Lohra M. Miller, Benjamin E. Draper and Martin F. Jarrold*,

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IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-06

John T. Kelly*, Ashlee R. Swindle*, Kyle M. Samperton*, KateLyn Smith, Kaitlin Coopersmith and Patterson R. Nuessle,

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IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-06

Anne Brenner, and , J. D. Brookbank,

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Developing a Mass Spectrometry-Based Research and Teaching Program at a Primarily Undergraduate Institution 以质谱为基础的研究与教学计划在本科院校的发展。

IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-05 DOI: 10.1021/jasms.5c00180

Jay G. Forsythe*,

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Sensitivity vs Competing Proton Transfer Reactions: Addressing Key Parameters of Ion Chemistry in Ion Mobility Spectrometry 灵敏度与竞争质子转移反应：离子迁移率光谱中离子化学的关键参数。

IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-08-01 DOI: 10.1021/jasms.5c00161

Christoph Schaefer*, and , Stefan Zimmermann,

{"title":"Sensitivity vs Competing Proton Transfer Reactions: Addressing Key Parameters of Ion Chemistry in Ion Mobility Spectrometry","authors":"Christoph Schaefer*,  and , Stefan Zimmermann, ","doi":"10.1021/jasms.5c00161","DOIUrl":"10.1021/jasms.5c00161","url":null,"abstract":"Ion mobility spectrometers (IMS) are widely used in various gas sensing applications due to their high sensitivity and rapid analysis times. However, in complex gas mixtures, reactions between the protonated target analyte and interfering species can lead to discrimination of analytes with low gas basicity, reducing sensitivity or even making detection impossible. Operating IMS at low pressure and high reduced electric field strengths has been shown to mitigate these competing ion–molecule reactions. Therefore, in this work, we present a kinetic model to evaluate the effect of key operating parameters on the ion suppression caused by competing ion–molecule reactions, guiding the instrumental design of IMS. The results demonstrate that measures to reduce competing ion–molecule reactions, such as lowering the operating pressure or reaction time, also reduce sensitivity due to fewer ion–neutral collisions. However, in scenarios with high concentrations of interferents, the reduced effect of competing ion–molecule reactions is critical for detecting target analytes with low gas basicity, thereby enhancing sensitivity under such conditions. Based on these findings, decreasing operating pressure and reaction time or increasing reduced electric field strength are the most promising strategies to minimize competing reactions and, in complex chemical backgrounds, increase sensitivity.","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 9","pages":"1929–1939"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/jasms.5c00161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A Simple Method for Determining Charge Distributions, Potentials, and Electric Fields Associated with an Electrospray Capillary 测定电荷分布、电位和与电喷雾毛细管相关的电场的简单方法。

IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-07-31 DOI: 10.1021/jasms.5c00124

Lars Konermann*,

{"title":"A Simple Method for Determining Charge Distributions, Potentials, and Electric Fields Associated with an Electrospray Capillary","authors":"Lars Konermann*, ","doi":"10.1021/jasms.5c00124","DOIUrl":"10.1021/jasms.5c00124","url":null,"abstract":"The electric field emanating from an electrospray ionization (ESI) capillary triggers the formation of a Taylor cone and the release of charged droplets. Uncovering the properties of this electric field is essential for developing a comprehensive understanding of the ESI process. The field is the negative gradient of the electric potential. The potential can be obtained by numerically solving the Poisson or Laplace equations, e.g., by using commercial software packages. However, such software tools do not necessarily provide mass spectrometry practitioners (many of whom were trained as biological or analytical chemists) with an intuitive grasp of the underlying concepts. The current work proposes an equivalent but much simpler method for uncovering the electrostatic properties of an ESI source. We focus on a charged capillary with an adjacent counter electrode (the latter represents the mass spectrometer). Instead of solving differential equations, the algorithm developed here adjusts the charge distribution on the ESI capillary until the potential anywhere on the capillary surface is constant. By definition, this scenario defines electrostatic equilibrium. The counter electrode can be included via simple image charge arguments. Our algorithm correctly predicts charge accumulation at the narrow capillary outlet, giving rise to a strong and highly divergent electric field in this area. Application of the method to various capillary geometries provides insights into the effects of experimental parameters. The code developed here is not intended to replace existing software packages, but it may provide practitioners with a more intuitive understanding of basic electrostatic principles. In addition, the method should be helpful for designing improved molecular dynamics simulations of ESI emitters.","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 9","pages":"1877–1888"},"PeriodicalIF":2.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SynchroSep-MS: Parallel LC Separations for Multiplexed Proteomics SynchroSep-MS：多重蛋白质组学的平行LC分离。

IF 2.7 2区化学

Journal of the American Society for Mass Spectrometry Pub Date : 2025-07-30 DOI: 10.1021/jasms.5c00207

Noah M. Lancaster, Li-Yu Chen, Bingnan Zhao, Benton J. Anderson, Mitchell D. Probasco, Vadim Demichev, Daniel A. Polasky, Alexey I. Nesvizhskii, Katherine A. Overmyer, Scott T. Quarmby and Joshua J. Coon*,

{"title":"SynchroSep-MS: Parallel LC Separations for Multiplexed Proteomics","authors":"Noah M. Lancaster, Li-Yu Chen, Bingnan Zhao, Benton J. Anderson, Mitchell D. Probasco, Vadim Demichev, Daniel A. Polasky, Alexey I. Nesvizhskii, Katherine A. Overmyer, Scott T. Quarmby and Joshua J. Coon*, ","doi":"10.1021/jasms.5c00207","DOIUrl":"10.1021/jasms.5c00207","url":null,"abstract":"Achieving high throughput remains a challenge in MS-based proteomics for large-scale applications. We introduce SynchroSep-MS, a novel method for parallelized, label-free proteome analysis that leverages the rapid acquisition speed of modern mass spectrometers. This approach employs multiple liquid chromatography columns, each with an independent sample, simultaneously introduced into a single mass spectrometer inlet. A precisely controlled retention time offset between sample injections creates distinct elution profiles, facilitating unambiguous analyte assignment. We modified the DIA-NN workflow to effectively process these unique parallelized data, accounting for retention time offsets. Using a dual-column setup with mouse brain peptides, SynchroSep-MS detected approximately 16,700 unique protein groups, nearly doubling the peptide information obtained from a conventional single proteome analysis. The method demonstrated excellent precision and reproducibility (median protein %RSDs less than 4%) and high quantitative linearity (median R2 greater than 0.96) with minimal matrix interference. SynchroSep-MS represents a new paradigm for data collection and the first example of label-free multiplexed proteome analysis via parallel LC separations, offering a direct strategy to accelerate throughput for demanding applications such as large-scale clinical cohorts and single-cell analyses without compromising peak capacity or causing ionization suppression.","PeriodicalId":672,"journal":{"name":"Journal of the American Society for Mass Spectrometry","volume":"36 9","pages":"1979–1987"},"PeriodicalIF":2.7,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0