Mechanism of Copper Stress on Algae Determined Using Mass Spectrometry Molecular Network: Molecular Characteristics and Metabolite Identification.

IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Yunkun Qian, Shuqi Wu, Yanan Liu, Yanan Chen, Jun Guo, Dong An
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引用次数: 0

Abstract

Algae typically respond to environmental changes by regulating the production and release of metabolites that affect water quality and cause various environmental issues. In this study, we investigated the role of algal organic matter (AOM) in copper [Cu(II)] using high-resolution mass spectrometry and a molecular-network-based nontargeted screening. The abundance and activity of algae were inhibited after the addition of Cu(II). Lipids, proteins, lignins, condensed aromatic structures, CHO-only classes, and nitrogenous organic matter are the primary components of AOM. The addition of extracellular organic matter (EOM) and intracellular organic matter (IOM) promoted the generation of carbohydrates that bonded to Cu(II), thus weakening Cu(II) toxicity. Furthermore, 1006 and 589 unique formulas were observed in the Cu(II)-EOM and Cu(II)-IOM groups, respectively, illustrating that EOM and IOM can induce algae to produce different metabolites to resist Cu(II) stress. Six novel phosphatidylethanolamines (PEs) and three novel phosphatidylglycerols (PGs) were identified in the EOM of the Cu(II)-EOM group. Therefore, AOM addition enhanced the synthesis of novel low-unsaturation and palmitoylated PEs, thereby regulating the immune response of algal cells under Cu(II) stress. Overall, these results demonstrated that Cu(II) can perturb lipid utilization and storage, whereas algae can alleviate Cu(II) toxicity by synthesizing and secreting different lipids.

利用质谱分子网络确定铜胁迫对藻类的作用机制:分子特征和代谢物鉴定。
藻类通过调节代谢物的产生和释放来应对环境变化,这些代谢物会影响水质并引起各种环境问题。在这项研究中,我们利用高分辨率质谱和基于分子网络的非靶向筛选研究了藻类有机物(AOM)在铜[Cu(II)]中的作用。Cu(II)的加入抑制了藻类的丰度和活性。脂质、蛋白质、木质素、凝聚芳香结构、纯cho类和含氮有机物是AOM的主要成分。细胞外有机物(EOM)和细胞内有机物(IOM)的加入促进了与Cu(II)结合的碳水化合物的生成,从而减弱了Cu(II)的毒性。此外,Cu(II)-EOM组和Cu(II)-IOM组分别有1006和589个独特的配方,说明EOM和IOM可以诱导藻类产生不同的代谢物来抵抗Cu(II)胁迫。在Cu(II)-EOM组的EOM中鉴定出6种新的磷脂酰乙醇胺(PEs)和3种新的磷脂酰甘油(pg)。因此,添加AOM促进了新型低不饱和棕榈酰化pe的合成,从而调节了Cu(II)胁迫下藻细胞的免疫反应。综上所述,Cu(II)可以扰乱脂质利用和储存,而藻类可以通过合成和分泌不同的脂质来减轻Cu(II)的毒性。
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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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