{"title":"Effect of Asparagus saponins on HepG2 Apoptosis and Mitochondrial Membrane Potential and ROS Level","authors":"Yubin Ji, C. Ji, L. Yue, L. Lang","doi":"10.1109/ESIAT.2010.5568423","DOIUrl":"https://doi.org/10.1109/ESIAT.2010.5568423","url":null,"abstract":"To study the effect of Saponins of asparagus on HepG2 apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (Δψm) levels. Saponins of asparagus with different concentration was treated with HepG2 at different time, MTT assay was used to detect inhibitory rate, fluorescence staining was used to observe apoptosis morphology, flow cytometry was used to detect apoptosis rate and cell cycle, also ROS and Δψm were measured by flow cytometry. The results showed Saponins of asparagus inhibited cell proliferation, the IC50 on HepG2 was 172.3 mg/L. Apoptosis morphology was observed by fluorescence microscope. The cell cycle of HepG2 was arrested at S phase, G2/M phase percent decreased. After 72h the treated group appeared apoptosis peak, and apoptosis rate with high dose group 30.94 ± 1.74%; After 48h the ROS in treated group increased with high dose group 77.7 ± 4.5%; and Δψm decreased with high dose group 77.8 ± 1.9%. Saponins of asparagus can induce HepG2 cell apoptosis, which is caused by increasing ROS and decreasing Δψm of HepG2.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"72 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2010-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84122742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Climate Warming Impact and Adaptation Activities of Rice Plantation in the Northeast China","authors":"Y. Wang, Tan Xu","doi":"10.1109/ICBBE.2009.5162506","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5162506","url":null,"abstract":"It is calculated that the average and standard deviation of the accumulate temperature from May to September for the period 1960-1999 of the Northeast China. The result shows that heat resource in almost all of the Northeast China increased. According to the quantitative relation between heat resource and rice yield per-unit-area of different rice varieties of earliest ripe, early ripe, medium ripe, medium-late ripe and late ripe, the model is established to calculate the expected rice yield per-unitarea using the method of expectation evaluation of risk-benefit decision-making. It is found climate warming induced positive effects on rice production in Heilongjiang and Jilin provinces, and induced negative effects in most areas of Liaoning Province. Keywordsadaptation; climate warming; expected yield perunit-area; Northeast China","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"26 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81818791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Extended Iterative Nonlinear Regression Normalization for cDNA Gene Expression Data","authors":"Jianping Lu, Y. Wang","doi":"10.1109/ICBBE.2009.5162838","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5162838","url":null,"abstract":"cDNA microarray expression data is widely used to help biomedical research. The data must be normalized because of various error functioned interferences existed. This paper has discussed the normalization for supervised multi-class (phenotype) data. All the classes are the type of multi-sample. Also, a reasonable hybrid cross-phenotype normalization (CPN) algorithm based on iterative nonlinear regression (INR) is proposed for this kind of array data set. As a part of this CPN algorithm, how to obtain a ldquobaselinerdquo from samples within a class by a statistical way and dynamic decision of reference/floating sample are discussed. Finally, experimental result is presented. The method in this paper has practical significance. Specifically, it can be used as a novel feature selection in gene pattern recognition.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"22 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88946503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inhibition Action on Xantine Oxidase by Biflavonoids from Selaginella labordei","authors":"Li Li, Ke-li Chen, Jia-Cheng Xu, Xiang Lei","doi":"10.1109/ICBBE.2009.5162968","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5162968","url":null,"abstract":"Objective Xanthine oxidase inhibitory activities of the total biflavonoids and eight biflavonoid compounds isolated from the medicinal plants of Selaginella labordei have been investigated. Method The active compounds were screened out by measuring the inhibition rate and the 50% inhibitory concentration (IC\u0000 50\u0000) of each sample against xanthine oxidase (XOD) in vitro. Result Three kinds of biflavonoids were more active in inhibiting XOD than others, their inhibition rate was more than 85% at the concentration of 10 mugldrmL\u0000 -1\u0000. IC\u0000 50\u0000 of the total biflavonoids, robustaflavone, 2\",3\"- dihydro -3',3\"'- biapigenin and 2\",3\"-dihydroochnaflavone was 0.529, 0.162, 0.257, 0.254 mugldrmL\u0000 -1\u0000 respectively. Conclusion The three kinds of biflavonoids were active compounds in inhibiting XOD from Selaginella labordei, and the total flavonoids was the main active position.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"34 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87709331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shi-ying Zheng, J. Ge, Jun Zhao, D. Jiang, Hong Li
{"title":"Expression of Caspase-3 Gene in Gastric Adenocarcinoma Cell Line SGC-7901 via Ad-FasL","authors":"Shi-ying Zheng, J. Ge, Jun Zhao, D. Jiang, Hong Li","doi":"10.1109/ICBBE.2009.5162833","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5162833","url":null,"abstract":"[Abstract] Objective To investigate the relationship between caspase-3 expression and apoptosis in the gastric adenocarcinoma cell line SGC-7901 transfected with the adenovirus-mediated FasL gene. Methods The human gastric adenocarcinoma cell line SGC-7901 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured by flow cytometry, TUNEL, RT-PCR, and immunocytochemical assays. Results The expression of caspase-3 was low in the gastric adenocarcinoma cell line SGC-7901, but transfection of these cells with Ad-FasL enhanced the cellular expression of the caspase-3 gene and induced susceptibility to Fas/FasL-mediated apoptosis (25.4% vs. 7.2%, p < 0.001). Apoptosis exhibited a positive relationship with caspase-3 expression (γ = 0.647, p < 0.01) Conclusions The down-regulation of caspase-3 expression and resistance to Fas/FasL-mediated apoptosis in gastric adenocarcinoma cells were found to be related to the development and progression of the cells. FasL gene transfer may increase caspase-3 expression in gastric adenocarcinoma cells and enhance apoptosis in them. [Key words] membrane glycoproteins; caspases; gastric neoplasms/pathology; oncogenes; cell death","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"162 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77213658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experiment Study of Fluoride Desorption with Groundwater Infiltration in Soil","authors":"Zhongwei Zhao, Jian Zhao, Y. Zong","doi":"10.1109/ICBBE.2009.5162811","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5162811","url":null,"abstract":"Fluoride contamination transport and adsorption study is a hot subject of soil environment research. Dynamic adsorption, desorption and kinetics study can help us deeply understand the migration and transformation mechanism of fluoride in water and soil system, and provide theoretical basis for environmental assessment and treatment. This paper reveals the fluoride desorption law with the infiltration of groundwater. It concluded that vertical distribution and desorption process of fluoride ions is different with content of clay and grain diameter. It is not a simple linear process for the decreasing concentration of fluoride. As in practical engineering application, we should make a special water import project for fluoride dilution according to the soil properties.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"1 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76507939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Estimation of Ultrasound Attenuation and its Application to Tissue Heterogeneity Study Using Nonlinear Least Square Data Fitting","authors":"Xiaoying Li, Dong Liu","doi":"10.1109/ICBBE.2009.5162800","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5162800","url":null,"abstract":"The aim of this paper is to provide a local attenuation method with applications to tissue heterogeneity estimation based on the peaks of ultrasound echo envelop data. To handle the frequency shift in the broadband ultrasound system, we use nonlinear least squares (NLS) data fitting directly to the local amplitude changes due to the tissue attenuation. By looking at the convergence of the NLS method, e.g., the Levenberg-Marquardt (LM) algorithm, we proposed a technique to test the tissue heterogeneity locally. System calibrations have been presented to compensate the aperture growth and beam diffraction effects. Algorithms have been verified both in phantom and in vivo images. Results showed that the proposed method can have 3.8% relative error in estimating attenuation coefficients of phantom images in a broadband system. It also showed the possible tissue heterogeneity from in vivo image.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"58 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84922644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Min Wu, Ning Liu, Chuanyin Wei, Meiyan Xing, Shanjie Lou
{"title":"Sludge Stabilization of Vermifilter Andanalysis of Influence Factors","authors":"Min Wu, Ning Liu, Chuanyin Wei, Meiyan Xing, Shanjie Lou","doi":"10.1109/ICBBE.2009.5163405","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5163405","url":null,"abstract":"Under different operating modes, this paper studies on the sludge stabilization effect and of vermifilter in different medium, and analyzed efficiency factors of the sludge stability in filter. The results showed that after the treatment of vermifilter, the average sludge VSS/SS was smaller than 50%, the average VFA was 30-40%, and the average organism decomposition rate was up to 48-65%, so the system's whole effect was perfect. Filter medium was one of the most important factors which affect the function of sludge stabilization. Compared to silica sands, earthworms' sludge digestion efficiency in ceramic pellets was higher, and the system's whole effect was better. It was mostly due to the damage to earthworms caused by filter medium. The most suitable temperature for earthworms' sludge digestion efficiency was 23-28degC, and the whole effect was the best at the same condition. Since hydraulic overloading was somehow negative to earthworms' normal activities and its sludge digestion efficiency, it should be less than 6.00 m/d.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"11 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82282871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Novel Method Evaluating Vascular Resistance Based on Fourier Analysis for Pulse Waveform","authors":"Huifeng Yang, Qi Zhou, Jun Xiao","doi":"10.1109/ICBBE.2009.5162219","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5162219","url":null,"abstract":"This paper proposed a novel method evaluating vascular resistance (VR). Its aim was for convenience and accuracy of non-invasive detection of VR. New index was defined through Fourier analysis for pulse waveform being representative of differently physiological and pathological status. The trial result showed a good correlation between this index and K value and this paper provided a new and doable suggestion for the clinically non-invasive detection of VR.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"23 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76688852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Prediction of Pollution on Underground Water from Ash-Water of Power Plant Ash-Field","authors":"Peiyao Xu, Ya Zhang, Jiae Song, Zhirui Hao","doi":"10.1109/ICBBE.2009.5163642","DOIUrl":"https://doi.org/10.1109/ICBBE.2009.5163642","url":null,"abstract":"On the basis of analysis of ash-water from Baoding power plant, chemical components of underground water and the condition of environmental hydrology the prediction on pollution from ash-water permeation was carried out by applying one- dimensional homogeneous mathematical model. The results show that ash-water will not take bad influence on the water quality of water source of Baoding city to ash-field 5000m in the short time. Keywords-ash-water; underground water pollution; pollution prediction At present, the domestic power plants in china use widely ash-hydraulic transport system, buck and fly ash are stored together in ash yard. Since most of ash yards belong to half closed circulating or half open system, there isn't ash yard becoming the center of underground water reverse filler. At the same time, the various contaminants in ash water seep with ash water to underground water causing pollution of underground water of ash yard around area. After contaminants enter underground water and move and spread in water, which will cause secure dangerous for the quality of water source for the resident living in the ash-field. Therefore the scientific prediction of pollution on underground water from ash-water is a very meaningful job for the power environmental protection workers. The ash yard of Baoding power plant is working object; we analyze the content of each contaminant in ash-water and underground water, and at the same time make detailed investigation of the environmental hydrogeology condition of ash yard around. On this foundation applying one-dimensional homogeneous mathematical model predict the moving condition in underground water of the contaminant Cl- and COD which is very important in ash-water in order to offering scientific basis for preventing pollution on underground water from ash-water.","PeriodicalId":6430,"journal":{"name":"2009 3rd International Conference on Bioinformatics and Biomedical Engineering","volume":"51 1","pages":"1-3"},"PeriodicalIF":0.0,"publicationDate":"2009-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75579898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}