Expression of Caspase-3 Gene in Gastric Adenocarcinoma Cell Line SGC-7901 via Ad-FasL

2009 3rd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2009-07-14 DOI:10.1109/ICBBE.2009.5162833

Shi-ying Zheng, J. Ge, Jun Zhao, D. Jiang, Hong Li

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引用次数: 2

Abstract

[Abstract] Objective To investigate the relationship between caspase-3 expression and apoptosis in the gastric adenocarcinoma cell line SGC-7901 transfected with the adenovirus-mediated FasL gene. Methods The human gastric adenocarcinoma cell line SGC-7901 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured by flow cytometry, TUNEL, RT-PCR, and immunocytochemical assays. Results The expression of caspase-3 was low in the gastric adenocarcinoma cell line SGC-7901, but transfection of these cells with Ad-FasL enhanced the cellular expression of the caspase-3 gene and induced susceptibility to Fas/FasL-mediated apoptosis (25.4% vs. 7.2%, p < 0.001). Apoptosis exhibited a positive relationship with caspase-3 expression (γ = 0.647, p < 0.01) Conclusions The down-regulation of caspase-3 expression and resistance to Fas/FasL-mediated apoptosis in gastric adenocarcinoma cells were found to be related to the development and progression of the cells. FasL gene transfer may increase caspase-3 expression in gastric adenocarcinoma cells and enhance apoptosis in them. [Key words] membrane glycoproteins; caspases; gastric neoplasms/pathology; oncogenes; cell death

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Caspase-3基因在胃腺癌细胞SGC-7901中的表达

【摘要】目的探讨转染腺病毒介导FasL基因的胃腺癌细胞SGC-7901中caspase-3的表达与细胞凋亡的关系。方法用腺病毒介导的FasL基因转染人胃腺癌细胞SGC-7901。采用流式细胞术、TUNEL、RT-PCR和免疫细胞化学检测caspase-3的表达和凋亡形态学变化。结果在胃腺癌细胞SGC-7901中，caspase-3的表达水平较低，但转染Ad-FasL可增强caspase-3基因的表达，诱导Fas/ fasl介导的细胞凋亡易感性(25.4% vs. 7.2%， p < 0.001)。凋亡与caspase-3表达呈正相关(γ = 0.647, p < 0.01)结论caspase-3表达下调及对Fas/ fasl介导的凋亡的抵抗与胃腺癌细胞的发育进展有关。FasL基因转移可增加胃腺癌细胞中caspase-3的表达，促进细胞凋亡。【关键词】膜糖蛋白;还存在;胃肿瘤/病理学;致癌基因;细胞死亡

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2009 3rd International Conference on Bioinformatics and Biomedical Engineering

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