生物化学进展(英文)最新文献

{"title":"Fusarium lini Potential for the Biotransformation of Norandrostenedione and Evaluation of Urease and Chymotrypsin Properties of the Transformed Products","authors":"S. P. Fodouop, Alex Doris Kengni Mboussaah, Didiane Yemele Mefokou, A. Fowa, Mahwish Siddiqui, G. T. Kamsu, D. Gatsing, M. Choudhary","doi":"10.4236/ABC.2021.112006","DOIUrl":"https://doi.org/10.4236/ABC.2021.112006","url":null,"abstract":"Several androgenic steroids have been biotransformed \u0000by fungi into metabolites with numerous biological properties. Incubation of \u0000norandrostenedione (1) with Fusarium lini NRRL 2204 was carried out for the first time, yielding two new \u0000metabolites, 3,7β-dihydroxy-19-norandrost-1,3,5-trien-17-one (3) and 6α,10β,17β-trihydroxy-19-nor-4-androsten-3-one (4), along with three known compounds, 3-hydroxy-19-norandrost-1,3,5-trien-17-one \u0000(2), 10β, 17β-dihydroxy-19-nor-4-androsten-3-one (5) and 10β-hydroxy-19-nor-4- androsten-3,17-dione (6). Their structures were elucidated by extensive \u0000spectroscopic analyses, including 1D-, 2D-NMR, and HR-MS experiments. Substrate 1 and its derivatives 2-6 were evaluated in vitro for their urease and chymotrypsin inhibitory properties. \u0000Compounds 2 and 3 were found to have strong urease activity with \u0000IC50 = 23.7 ± 0.17 and 10.2 ± 0.28 μm, respectively, as compared \u0000to the standard drug thiourea (IC50 = 21.6 ± 0.12 μm). Compounds 4, 5 and 6 showed good chymotrypsin activity with IC50 values of \u00006.4 ± 0.19, 15.6 ± 0.46 and 18.4 ± 0.65 μm, respectively, as compared to standard chymostatin \u0000with IC50 = 5.7 ± 0.14 μm. These transformed metabolites may form the basis for the future \u0000development of new drugs against ulcer, inflammation, bacterial and viral \u0000diseases.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47210624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Hemisynthetic Oleanane Saponin with Antimicrobial Activities","authors":"Cyrille Ngoufack Tagousop, J. Tamokou, Léonel Donald Tsamo Feugap, D. Harakat, L. Voutquenne-Nazabadioko, D. Ngnokam","doi":"10.4236/ABC.2021.111001","DOIUrl":"https://doi.org/10.4236/ABC.2021.111001","url":null,"abstract":"A new hemisynthetic oleanane saponin: 4',2\",3\",4\",6\"-penta-O-acetyl-6'-O- methyl-3-O-β-D-glucopyranosyl-(1→2)-β-D-glucuronopyranosyl \u0000oleanolic acid (2) was obtained after acetylation and methylation reaction on 3-O-β-D- glucopyranosyl-(1→2)-β-D-glucuronopyranosyl oleanolic acid (1). Its structure was established by extensive analysis of 1D-(1H, 13C), \u00002D-(COSY, HSQC and HMBC) NMR data in conjunction with mass spectrometry (HR- TOFESIMS). \u0000The evaluation of antimicrobial activities using microdilution method showed \u0000that, reaction product (2) presented \u0000the lowest values of MIC against E. coli (MIC = 16 μg/mL), S. aureus (MIC = 8 μg/mL), C. tropicalis (MIC = 16 μg/mL) C. albicans (MIC = 8 μg/mL) compare to the substrate (1) against the same microbial \u0000strains: E. coli (MIC = 32 μg/mL), S. aureus (MIC = 16 μg/mL), C. tropicalis (MIC = 32 μg/mL), C. albicans (MIC = 16 μg/mL). These results indicate that, acetylation \u0000and methylation reactions of compound 1 increase its antimicrobial \u0000activities against the tested microorganisms.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46326515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Rapid Diagnostic Tests Algorithms in Transfusion Medicine Setting","authors":"M. Coulibaly, B. Maiga, D. Samaké, M. Diawara, M. Traoré, Valentin Sagara, B. Traoré, O. Guindo, A. Dolo","doi":"10.4236/ABC.2021.111005","DOIUrl":"https://doi.org/10.4236/ABC.2021.111005","url":null,"abstract":"Background: The rapid diagnostic tests play a pivotal role in the screening of viral \u0000markers in blood qualification for transfusion in limited resource setting. \u0000Therefore, it is important to assess their analytical performances to ensure \u0000their proper functioning. Material and Methods: We performed \u0000a cross- sectional study by successive recruitment to assess the diagnostic value \u0000of rapid diagnostic tests algorithms using ELISA as a reference test. A total \u0000of 661 blood from donors were enrolled for this study. Rapid Diagnostic Tests \u0000(RDTs) and ELISA tests were performed for each sample by a couple of \u0000double-blinded biotechnologists. Data were collected on case report form and \u0000captured in Microsoft Excel then the file was imported and analyzed using R \u0000software version 4.0.3. Results: The diagnostic accuracy for the \u0000algorithms are summarized in Table 1. For HIV-algorithm, the \u0000internal validity parameters were as follow: sensitivity (sens) 99.0% (95% CI = \u000097.8, 99.5); specificity (spec) 98.3% (95% CI = 90.9, 99.7); positive \u0000likelihood ratio (PLR) 57.4 (95% CI = 8.2, 401.0); negative likelihood ratio \u0000(NLR) 0.01 (95% CI = 0.0005, 0.02); diagnostic odd ratio (DOR) 4710. HBV-Ag/Ab \u0000RDTs achieve the following diagnostic accuracy: sens 99.7% (95% CI = 98.3, \u000099.9); spec 98.8% (95% CI = 96.9, 95.5); PLR 81.8 (95% CI = 30.9, 217.0); NLR \u00000.003 (95% CI = 0.0004, 0.02); DOR 14,110. The \u0000analytical performances of HCV-Ab RDTs were as follow: sens 98.7% (95% CI = \u000097.5, 99.4); spec 93.1% (95% CI = 78.0, 98.1); PLR 14.3 (95% CI = 3.8, 54.5); \u0000NLR 1.5 (95% CI = 0.8, 2.8); DOR 962.6. The parameters evaluating the external \u0000validity of RDTs screening for the three viral markers when the theorical \u0000prevalence was 5% are summarized in Figure 3. At the prevalence , 99.99% and 99.94%. At the same prevalence, we found \u0000the following Positive Predictive Values (PPV) 70.82%, 77.59% and 37.35% for \u0000HIV-Ag/Ab RDTs, HBV-Ag RDTs and HCV-Ab RDTs algorithms, respectively. The \u0000overall areas under the received operating characteristic (ROC) curves were \u000098.6%, 99.2% and 99.2%; 95.9% for HIV-Ag/Ab RDTs, HBV-Ag RDTs and HCV-Ab RDTs \u0000algorithms, respectively. Conclusion: RDTs algorithms can play a pivotal \u0000role in the screening of HIV-Ab/Ag, HBs-Ag in the setting of resources \u0000limited-countries where financial and technical expertise shortages are a \u0000standard fare. However, their use for diagnostic purposes must be done with \u0000great caution and the result must necessarily be confirmed with an ELISA or \u0000molecular technique particularly for HCV-RDTs algorithm which achieved an NLR \u0000value > 0.1.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44480417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation, Identification, Isolation and Characterization of Degradation Product of Osimertinib Tablets by UPLC-QTOF-MS/MS and NMR: Evaluation of In-Silico Safety Assessment for Osimertinib (OSM) and Degradation Product (DP)","authors":"Arun D. Bhutnar, Seema R. Saple, V. Vaidya","doi":"10.4236/ABC.2021.111003","DOIUrl":"https://doi.org/10.4236/ABC.2021.111003","url":null,"abstract":"The present work encompasses identification and characterization of major \u0000degradation product (DP) of OSM observed in base hydrolytic stress study. The \u0000separation of DP was carried out on a non-polar stationary phase by using high-performance \u0000liquid chromatography system (HPLC). Using waters X-bridge (250 mm × 4.6 mm, 5 \u0000μm) C18 column with gradient elution program. For the characterization study, \u0000stress samples were subjected to HPLC and UPLC-QTOF-MS/MS and based on mass \u0000fragmentation pattern, plausible structure was deduced. Further, the DP \u0000was isolated using semi-prepara- tive \u0000liquid chromatography and concentrated the fractions using lyophilization. The isolated DP was subjected to extensive \u00001D (1H, 13C, and DEPT-135) and 2D (COSY, HSQC and HMBC) nuclear magnetic \u0000resonance (NMR) studies to authenticate the structure. The impurity was \u0000unambiguously named as N-(2-((2-(dimethylamino)ethyl)(methyl)amino)-4-metho-xy-5-((4-(1-methyl-1H-indol-3-yl)pyrimidin-2-yl)amino)phenyl)-3-methoxypropanamide. Add- itionally, the In-Silico structure activity relation (QSAR) assessed through statistical based software’s DEREK NexusTM, \u0000and MultiCASE, Case UltraTM widely accepted and respected software’s for DP and \u0000OSM.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41982238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Central Role of Innate B-Cell Immunity in Cancer Prevention","authors":"N. Tupitsyn","doi":"10.4236/ABC.2021.111002","DOIUrl":"https://doi.org/10.4236/ABC.2021.111002","url":null,"abstract":"There are some forgotten items. There is a specific way to eradicate cancer cells at the very early stage of their appearance. Natural humoral \u0000immunity with CD5+ B-cell produced pentameric IgM to cancer associated glycans \u0000normally to eliminate arising cancer cells. This branch of innate immunity is \u0000decreasing with age and that is a basis for selective immunodeficieny which may \u0000be corrected. This area of research was well studied and proved by the team of \u0000Prof. Vollmers (Germany), but then forgotten for about 15 years.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49284575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, Characterization, Antimicrobial and DNA Binding Studies of a Tetradentate N2O2 Amino Acid Schiff Base and Its Coordination Compounds","authors":"T. Aiyelabola, D. Otto, J. Jordaan, E. Akinkunmi, I. Olawuni","doi":"10.4236/ABC.2021.111004","DOIUrl":"https://doi.org/10.4236/ABC.2021.111004","url":null,"abstract":"Aminoethanoic acid undergoes condensation with 1,4-benzenedicarboxaldehyde to form an O, N, N, O donor Schiff base, N,N'-di(carboxymethylene) terephthalaldehyde, \u0000Ligand L. Coordination compounds of this Schiff base using Ni (II), Cu (II), VO (IV) and Co (II) were then \u0000obtained in-situ. The Schiff base and the complexes were evaluated \u0000for their antimicrobial and DNA binding abilities. Molecular docking studies of \u0000the ligand and synthesized compounds were also carried out. Evidence for the \u0000formation of the Schiff base coordination compounds and the coordinating atoms \u0000was obtained from 1H NMR, infrared and ultraviolet spectral data, \u0000EDX, EDTA complexometric titration and magnetic susceptibility measurement. The \u0000results obtained are consistent with octahedral geometry for Ni (II) complex, the \u0000metal ion coordinating to one molecule of Ligand L and with additional coordination with two oxygen atoms of two molecules of \u0000the solvent. A square-planar geometry was suggested for both Co (II), and Cu \u0000(II) complexes and a five-coordinate, square pyramidal geometry for the VO (IV) \u0000complex. The results further indicated that the carboxylic acid of Ligand L was \u0000not deprotonated both in the free base and also the complexes. In addition, the \u0000results showed that Compound 2 elicited the best antimicrobial activity \u0000potential. Generally, the compounds exhibited considerable good affinity to \u0000CT-DNA.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42532293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of Quantitative Changes in Bone Marrow Lymphocyte Subpopulations with Some Rhabdomyosarcoma Prognosis Factors in Children","authors":"O. P. Kolbatskaya, T. V. Gorbunova, S. Chulkova, A. Khachatryan, N. Tupitsyn, V. Polyakov","doi":"10.4236/abc.2021.115016","DOIUrl":"https://doi.org/10.4236/abc.2021.115016","url":null,"abstract":"","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70475284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fruit-Based Non-Dairy Beverage: A New Approach for Probiotics","authors":"I. Gomes, A. Venâncio, J. P. Lima, O. Freitas-Silva","doi":"10.4236/abc.2021.116021","DOIUrl":"https://doi.org/10.4236/abc.2021.116021","url":null,"abstract":"The growing interest of consumers in using foods that improve health has motivated researchers and the food industry to develop new functional products such as foods with probiotics. Probiotic cultures, for example, from lactic acid bacteria and bifidobacteria have been highlighted for their ability to promote balance in the intestinal microbiota as well as other benefits such as anticarcinogenic and antimutagenic effects, reduced plasma cholesterol levels, decreased symptoms of lactose intolerance, and stimulation of the immune response. Traditionally, probiotics are incorporated into dairy products. How-ever, because of the growing number of individuals affected by lactose intolerance and/or vegans, other food matrices have been studied as potential carriers for these microorganisms. Considering all the facts mentioned above, cereals, legumes, fruits, and vegetables could be potential substrates, where probiotic bacteria can be used for the development of non-dairy beverages. This review aimed to highlight the research carried out on 1) probiotic mi-cro-organisms, including the more recent reclassification according to their phylogenetic position, 2) probiotic beverages from non-dairy sources which emerged as an alternative for lactose-intolerant consumers and, 3) the aspects of improving the gut microbiota.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70475807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adsorption of Toluene on Film Membranes of Chitosan/Nа-CMC","authors":"Abdullaev Nodirkhon Juraxonovich, Kodirkhonov Murodkhon Rashidhonovich","doi":"10.4236/abc.2021.116020","DOIUrl":"https://doi.org/10.4236/abc.2021.116020","url":null,"abstract":"The sorption properties of films on bases of chitosan and sodium carboxyl methylcellulose (Na-CMC) with toluene have been checked. The sorption rate, sorption thermodynamics and isothermal properties of toluene molecules in chitosan/Na-CMC films are analyzed by adsorption-calorimetric method. Thus, it was found that toluene is adsorbed on the chitosan/Na-CMC film by the same patterns of sorption of aromatic compounds, due to the process of penetration of toluene molecules into the network of chitosan macromolecules and this is preceded by the absorption process by the surface of the chitosan/Na-CMC film.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70475751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Study and Comparative Antioxidant Activity of Extracts from Aerial Parts of Chenopodium ambrosioides Linn. (Chenopodiaceae)","authors":"Gneiny Whad Tchani, Kossi Sitsopé Agbeme, Kokou Agbékonyi Agbodan, G. Baba, K. Kpegba","doi":"10.4236/abc.2021.115015","DOIUrl":"https://doi.org/10.4236/abc.2021.115015","url":null,"abstract":"","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70475270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}