生物化学进展(英文)最新文献_第10页

Synthesis, Characterizations, Biological, and Molecular Docking Studies of Some Amino Acid Schiff Bases with Their Cobalt(II) Complexes 一些氨基酸席夫碱及其钴（II）配合物的合成、表征、生物学和分子对接研究

生物化学进展(英文) Pub Date : 2017-09-22 DOI: 10.4236/ABC.2017.75013

M. Salama, Soad Ahmed, Safaa S. Hassan

引用次数: 10

Re-Thinking Biology—I. Maxwell’s Demon and the Spontaneous Origin of Life 反思上学期。麦克斯韦妖与生命的自发起源

生物化学进展(英文) Pub Date : 2017-09-22 DOI: 10.4236/ABC.2017.75012

C. Busby, C. Howard

引用次数: 1

Comparison of Serum Phospholipase A2 Activities of All Known Extant Crocodylian Species 所有已知现存鳄鱼种血清磷脂酶A2活性的比较

生物化学进展(英文) Pub Date : 2017-08-25 DOI: 10.4236/ABC.2017.74010

Mark Merchant, C. McAdon, S. Mead, Justin McFatter, C. McMahan, R. Griffith, Christopher M. Murray

引用次数: 1

Citrus jabara Extracts Suppress MUC5AC Mucin Production in Human Lung Epithelial Cells 柑桔提取物抑制人肺上皮细胞MUC5AC粘蛋白的产生

生物化学进展(英文) Pub Date : 2017-06-29 DOI: 10.4236/ABC.2017.73009

J. Iwashita, N. Iguchi, A. Takashima, Daisuke Watanabe, Kimihiko Sano, Masahiko Ishikuro, K. Hata, J. Murata

引用次数: 4

Fc-Epsilon Receptor (CD23) Expressing Follicular Dendritic Cells as a Main Prognostic Factor in Follicular Lymphoma 表达滤泡树突状细胞的Fc-Epsilon受体(CD23)是滤泡性淋巴瘤的主要预后因素

生物化学进展(英文) Pub Date : 2017-04-25 DOI: 10.4236/ABC.2017.72007

N. Falaleeva, E. Osmanov, N. Tupitsyn

{"title":"Fc-Epsilon Receptor (CD23) Expressing Follicular Dendritic Cells as a Main Prognostic Factor in Follicular Lymphoma","authors":"N. Falaleeva, E. Osmanov, N. Tupitsyn","doi":"10.4236/ABC.2017.72007","DOIUrl":"https://doi.org/10.4236/ABC.2017.72007","url":null,"abstract":"Fc-epsilon receptor (CD23)-expressing follicular dendritic cells is a main prognostic factor in follicular lymphoma. Falaleeva N. A., Osmanov E. A., Tupitsyn N. N. Federal State Budgetary Institute N. N. Blokhin Russian Cancer Research Center, Health Ministry of Russian Federation, Moscow, Russia SUMMARY Follicular dendritic cells, expressing FceRII or CD23 (FceRIIFDCs) as a component of non-tumor environment have been studied in 232 follicular lymphoma (FL) patients. FceRIIFDCs were found in 87.5% of follicular lymphoma cases and were associated with a nodular pattern of tumor growth (p = 0.000), but not the cytological grade of lymphoma. There were no associations of FceRIIFDC with clinical prognostic factors (FLIPI indices) or with bone marrow involvement in FL patients by histology. The presence of FceRIIFDCs in tumor tissue was an independent prognostic factor according to treatment results, i.e. frequency of CR, duration of OS and PFS. Bone marrow involvement significantly worsened the prognosis in FceRIIFDC-positive group of patients. We suggest a new prognostic index (FDC-IP) that allows biochemical identification of the following patient groups: FceRIIFDC-positive patients without bone marrow involvement (good prognosis), FceRIIF-DC-positive patients with bone marrow involvement (intermediate prognosis), FceRIIFDC-negative patients (poor prognosis). These 3 groups significantly differ (p = 0.000) both in OS and in PFS. This is the first evidence of the possibility to assess tumor behavior and treatment results in FL according to lymphoma biochemical and other than clinical parameters.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":"07 1","pages":"107-121"},"PeriodicalIF":0.0,"publicationDate":"2017-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41710028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Applications of a Calmodulin-Based Fusion Protein System for the Expression and Purification of WW and Zinc Finger Modules. 基于钙调素的WW和锌指蛋白表达纯化融合蛋白体系的建立与应用。

生物化学进展(英文) Pub Date : 2017-04-25 DOI: 10.4236/ABC.2017.72006

Christopher G Toomey, D. Weiss, A. Chant, Megan M. Ackerman, B. Ahlers, Y. Lam, Christopher Ricciardi, D. Bourne, Christina M. Kraemer-Chant

{"title":"Development and Applications of a Calmodulin-Based Fusion Protein System for the Expression and Purification of WW and Zinc Finger Modules.","authors":"Christopher G Toomey, D. Weiss, A. Chant, Megan M. Ackerman, B. Ahlers, Y. Lam, Christopher Ricciardi, D. Bourne, Christina M. Kraemer-Chant","doi":"10.4236/ABC.2017.72006","DOIUrl":"https://doi.org/10.4236/ABC.2017.72006","url":null,"abstract":"Calmodulin from Homo sapiens is an α-helical calcium-binding protein that expresses to high levels in E. coli. When the N-terminus of a calmodulin variant is bound to Ca2+, it undergoes a conformational change, exposing hydrophobic pockets. This property can be utilized for purification purposes, as these pockets bind to phenyl sepharose resin with high affinity. Washing with EDTA chelates the Ca2+ ions from the protein, inducing a conformational change back to the more folded state and eluting the protein from the column. We describe herein the use of a protein expression and purification technique using the calmodulin variant and a short linker for proteolytic cleavage by the mutant NIa-Pro tobacco etch virus protease. We have shown this approach to be useful in obtaining purified quantities of various small proteins that could not be expressed using other methods, including high enough concentrations of a designed WW domain protein for NMR structural analysis. We have also obtained promising results on the usefulness of this procedure to express and purify zinc finger proteins without the addition of zinc ions or other cofactors.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":"7 2 1","pages":"89-106"},"PeriodicalIF":0.0,"publicationDate":"2017-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45790509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physico-Chemical and Microbiological Analysis of Water of the “Presa De Los Patos” in the Desierto De Los Leones National Park, Mexico 墨西哥Desierto De Los Leones国家公园“Presa De Los Patos”水体的理化和微生物学分析

生物化学进展(英文) Pub Date : 2017-04-25 DOI: 10.4236/ABC.2017.72008

Luis Raúl Gutiérrez-Lucas, Luz María Chávez-Ramírez, L. Arregui

{"title":"Physico-Chemical and Microbiological Analysis of Water of the “Presa De Los Patos” in the Desierto De Los Leones National Park, Mexico","authors":"Luis Raúl Gutiérrez-Lucas, Luz María Chávez-Ramírez, L. Arregui","doi":"10.4236/ABC.2017.72008","DOIUrl":"https://doi.org/10.4236/ABC.2017.72008","url":null,"abstract":"The Desierto de los Leones National Park is a place that features recreational activities for residents and visitors to Mexico City. The Presa de los Patos (Ducks Dam) is one of the many bodies of water that are present in the park. By means of physico-chemical and microbiological analysis, it was determined that the water of the Presa de los Patos cannot be considered potable. The pH range was between 5.5 - 5.9. The turbidity exceeded the values established in the standards (10.7 - 32.3 Nephelometric Turbidity Units (NTU’s)). Chemical Oxygen Demand (COD) showed that large amounts of oxygen are needed to oxidize organic matter (106 - 450 mg/L). The amount of coliforms and fecal coliforms exceeds the values established by the standards used (total coliforms 98 - 956 Colony Forming Units (CFU’s), fecal coliforms 78 - 807 CFU’s). Five bacterial genera (Escherichia coli, Pseudomona, Klebsiella, Shigella and Salmonella) were isolated and identified. Most of the bacteria isolated were resistant to ampicillin (90%), while 25% were resistant to ciprofloxacin. The water consumption of the Presa de los Patos is a great risk for the habitants and visitors of the park.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":"07 1","pages":"122-138"},"PeriodicalIF":0.0,"publicationDate":"2017-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45754046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A Thiazole Analogue Exhibits an Anti-Proliferative Effect in Different Human Carcinoma Cell Lines and Its Mechanism Based on Molecular Modeling 一种噻唑类似物在不同人类癌细胞系中表现出抗增殖作用及其基于分子模型的机制

生物化学进展(英文) Pub Date : 2017-02-08 DOI: 10.4236/ABC.2017.71005

Hatem K. Amin, Amr M. El-Araby, Sameh Eid, T. Nasr, S. Bondock, O. Leheta, M. Dawoud

{"title":"A Thiazole Analogue Exhibits an Anti-Proliferative Effect in Different Human Carcinoma Cell Lines and Its Mechanism Based on Molecular Modeling","authors":"Hatem K. Amin, Amr M. El-Araby, Sameh Eid, T. Nasr, S. Bondock, O. Leheta, M. Dawoud","doi":"10.4236/ABC.2017.71005","DOIUrl":"https://doi.org/10.4236/ABC.2017.71005","url":null,"abstract":"Purpose: Aim of this study is to assess the anti-proliferative effect of the thiazole analogue (5-acetyl-4-methyl-2-(3-pyridyl) thiazole) with different human carcinoma cell lines and to postulate its possible mechanism of action using molecular modeling. Methods: Three different human carcinoma cell lines were used namely hepatocyte carcinoma (HEPG2), breast adenocarcinoma (MCF7) and colon cancer (HCT116). Molecular docking simulations for tested thiazole analogue and its virtual analogues against the cytochrome P-450 2A6 enzyme and mutated SOD were performed. Results: Cell lines cytotoxicity revealed that the tested thiazole analogue exerts a significant anti-proliferative activity in all the used human carcinoma cell lines with a pronounced anti-proliferative effect in liver carcinoma cell line HEPG2 (IC50 = 23.8 μg/ml) whereas the anti-proliferative effect in colon carcinoma and breast cancer cell lines was poor with IC50 = 50 μg/ml and IC50 > 50 μg/ml respectively. The postulated mechanism of action revealed the high affinity to inhibit SOD and CYP2A6 enzymes in the liver. Conclusion: The thiazole analogue (5-acetyl-4-methyl-2-(3-pyridyl)thiazole) is a potential liver specific anticancer agent capable of interfering with both apoptotic signaling pathway and the free radical processing in liver which leads to more studies on liver cancer from different perspective rather than the apoptotic signaling pathway.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":"07 1","pages":"76-87"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42343301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Mitochondrial Membranes Restitution Proceeds via Vesicular Import from ER and Cytosol. Counterparts’ Resemblances and Variances in Mitochondria and Golgi Pathways 线粒体膜的恢复通过内质网和细胞质的水泡输入进行。线粒体和高尔基体通路的异同

生物化学进展(英文) Pub Date : 2017-02-08 DOI: 10.4236/ABC.2017.71001

A. Slomiany, B. Slomiany

{"title":"Mitochondrial Membranes Restitution Proceeds via Vesicular Import from ER and Cytosol. Counterparts’ Resemblances and Variances in Mitochondria and Golgi Pathways","authors":"A. Slomiany, B. Slomiany","doi":"10.4236/ABC.2017.71001","DOIUrl":"https://doi.org/10.4236/ABC.2017.71001","url":null,"abstract":"The processes of mitochondrial restitution are controlled by nuclear genes that encode proteins synthesized in ER and cytosol and delivered as organelle- and membrane-specific transport vesicles. The analysis of the transporters recovered from inner mitochondrial space (Mitosol) revealed that the ER-synthesized mitochondria-specific transport vesicles consist of two carriers, one remaining in outer mitochondrial membrane (OMM), and the other that transfers specific membrane segments to the inner mitochondrial membrane (IMM). The ER-assembled and IMM-committed membrane segments, while first integrated into OMM, undergo intra-mitochondrial lipid modification reflected in the synthesis of cardiolipin (CL) and inversion into Mitosol with load of IMM associated cytosolic proteins. Then, the CL-bedecked vesicles are released from OMM to Mitosol and upon contact with IMM fuse with the membrane, and the release of cytosolic cargo ensues. While ER-assembled mitochondria-specific transport vesicles fuse with OMM with the aid of the cytosolic, phosphatidylglycerol (PG)-specific phospholipase A2 (PLA2), the Mitosol-contained CL-specific PLA guides vesicles fusion with IMM. The described path of translocation of the membrane segments and the cytosol synthesized proteins into the designated mitochondrial compartments sustains growth and identity of OMM, IMM, maintains protein delivery for intra-mitochondrial lipid and protein modification in Mitosol, and ensures conformity of the cytosolic proteins cargo delivered to matrix.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":"07 1","pages":"1-26"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41506519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Lipid-Protein Microinclusions in the Morphological Structures of Organelle Membranes Studied by Fluorescent Confocal Microscopy 荧光共聚焦显微镜研究细胞器膜形态结构中的脂质蛋白微包裹体

生物化学进展(英文) Pub Date : 2017-02-08 DOI: 10.4236/ABC.2017.71003

M. Chernyshov, V. Nurminsky, N. V. Ozolina

{"title":"Lipid-Protein Microinclusions in the Morphological Structures of Organelle Membranes Studied by Fluorescent Confocal Microscopy","authors":"M. Chernyshov, V. Nurminsky, N. V. Ozolina","doi":"10.4236/ABC.2017.71003","DOIUrl":"https://doi.org/10.4236/ABC.2017.71003","url":null,"abstract":"Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles. During this study, identification of lipid microinclusions having the form of such lipid-protein structural microformations as lipid-protein microdomains, vesicles and membrane tubular structures (cytoplasmic transvacuolar strands and nanotubes) located in organelle membranes or bound up with them was conducted. Such membrane probes as laurdan, DPH, ANS and bis-ANS were used. Comparison of fluorescence intensity of these membrane probes was conducted. This investigation of the morphological properties of lipid-protein structural microformations was accompanied with analysis of 1) the phase state and 2) dynamics of microviscosity variations in the membrane elements of isolated plant cell organelles. Distributions of laurdan fluorescence generalized polarization (GP) values for the membrane on the whole and for the intensively fluorescing membrane segments were obtained. It was discovered that the microviscosity of intensively fluorescing membrane segments essentially differed from the microviscosity of the rest part of the membrane. In conclusion, some results of the study of peculiar properties of lipid-protein structural microformations related to the structure of organelle membranes and the discoveries made in this investigation are discussed.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":"07 1","pages":"42-59"},"PeriodicalIF":0.0,"publicationDate":"2017-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43157363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2