Biomaterials Translational最新文献

{"title":"Cold-sintered bioceramics for medical applications: State of the art and further perspectives.","authors":"Miodrag J Lukic, Denis Gebauer, Bin Li, Song Chen","doi":"10.12336/bmt.25.00157","DOIUrl":"10.12336/bmt.25.00157","url":null,"abstract":"<p><p>Cold sintering has recently emerged as a promising approach for preparing dense ceramic materials and composites at low temperatures. It relies on utilizing transient, typically externally introduced, liquid phases to accelerate material diffusion and densification under applied pressure. Cold-sintered bioceramics, especially those prepared at temperatures below 100°C, may open up numerous possibilities, not only in producing dense ceramics with refined microstructural properties and reduced time/energy costs, but also in developing multifunctional platforms containing bioactive compounds, therapeutics, growth factors, and signaling molecules for enhanced and targeted biological responses. Cold sintering in the presence of liquids inherently involves dissolution and nucleation, which become particularly intricate under applied pressures and elevated temperatures. Pseudo bio-mineralization, an auspicious approach for tailoring synthetic bone grafts toward targeted mechanics, may serve as a viable route for enhancing the densification mechanisms inherent to cold sintering. We have carefully analyzed the current state of the art in cold-sintered bioceramics and the results achieved, with a focus on the chemistry of the employed liquids and the corresponding changes upon sintering, the selection of transient phases, and mineral nucleation, while also addressing the potential for developing new biomaterials. Despite the widely accepted classical dissolution-precipitation strategy, no clear roadmap can yet be defined regarding the type and amount of liquid phase that should be applied, at least in the case of hydroxyapatite (HAp) densification-the most important representative of calcium phosphates. We strongly advocate the use of water as the transient liquid of choice in the cold sintering of HAp-based bioceramics, instead of strong acids/bases, and emphasize the importance of understanding the various processes and parameters that govern and connect solution chemistry to mineral nucleation. This understanding will enable the advancement of cold sintering protocols in a target-oriented manner, and we provide perspectives on future developments, including practical advice.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 4","pages":"389-401"},"PeriodicalIF":0.0,"publicationDate":"2025-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12852070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146108672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shellac-based encapsulation model incorporating calcium fluoride for oral care applications.","authors":"Piyada Dissara, Chompunuch Sukcheep, Sanong Ekgasit, Prompong Pienpinijtham, Apichat Phengdaam","doi":"10.12336/bmt.25.00099","DOIUrl":"10.12336/bmt.25.00099","url":null,"abstract":"<p><p>Fluoride-releasing systems are widely incorporated into dental products to prevent caries; however, fluoride ions readily interact with calcium compounds in oral formulations, resulting in deactivation and reduced effectiveness. To overcome this limitation, a simple extrusion method was designed to fabricate shellac-based biopolymer beads capable of encapsulating fluoride. The resulting beads had an average diameter of 2.54 ± 0.22 mm, with theoretical analysis supporting particle size dependence on extrusion tube diameter. Structural and stability characterization was conducted using optical and electron microscopy, elemental analysis, vibrational spectroscopy, and thermal profiling, confirming <i>in situ</i> calcium fluoride formation and effective pore sealing. Key formulation parameters-including shellac concentration, dissolution time, bead formation time, and hardening time-were optimized to improve bead morphology and fluoride retention. An <i>in situ</i> blocking strategy, involving controlled precipitation of calcium fluoride within the bead matrix during formulation, was implemented to minimize fluoride diffusion and enhance long-term stability. This approach achieved 70% active fluoride retention over 90 days. The proposed encapsulation model holds promise for applications in toothpaste and other dental care products.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"7 2","pages":"312-320"},"PeriodicalIF":0.0,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13585498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149003034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microneedles in biomedicine: Innovations, challenges, and future prospects.","authors":"Xinrui Li, Chi Zhang, Yuxin Zhang, Zhijing Liu, Jiaxin Li, Ying Meng, Peng Zhang","doi":"10.12336/bmt.25.00122","DOIUrl":"10.12336/bmt.25.00122","url":null,"abstract":"<p><p>The effective delivery of therapeutic drugs is fundamental to modern medical practice. However, conventional administration methods, primarily oral and parenteral injection, exhibit numerous limitations, including the suboptimal bioavailability of macromolecules and challenges related to patient compliance. The advent of microneedle (MN) technology is reshaping strategies in the biomedical field, effectively overcoming the constraints of traditional drug delivery and diagnostic approaches. Research indicates that MNs can penetrate the stratum corneum to form transient microchannels, facilitating the transdermal delivery of therapeutic agents while bypassing gastrointestinal and hepatic barriers. This customizable and personalized drug delivery system holds significant potential for clinical application. Beyond drug delivery, MNs also have the capacity to transform healthcare models through real-time biomarker monitoring enabled by contact with interstitial fluid. This technology demonstrates considerable promise in managing chronic conditions such as diabetes, while also opening avenues for applications in vaccination, tissue regeneration, and cancer therapy. Recent innovations include the development of stimulus-responsive MNs for precision medicine and their integration with wearable devices to achieve closed-loop therapeutic diagnostics. Despite the substantial promise of this field, challenges remain regarding clinical translation, particularly in relation to biocompatibility, mechanical strength, and drug stability. This review outlines MN classifications, design principles, and applications, emphasizing their expanding role not only in healthcare but also in precision medicine, global health, and food safety. By overcoming current barriers and integrating emerging technologies, MNs have the potential to transform diagnostic and therapeutic paradigms, delivering scalable, patient-centered solutions to a broad range of biomedical challenges.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 4","pages":"373-388"},"PeriodicalIF":0.0,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12852078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146108715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of CRISPR-artificial intelligence theranostics: Current and emerging applications.","authors":"Prerna Vats, Bhavika Baweja, Chainsee Saini, Atar Singh Kushwah, Ashok Kumar, Sandeep K Srivastava, Rajeev Nema","doi":"10.12336/bmt.25.00106","DOIUrl":"10.12336/bmt.25.00106","url":null,"abstract":"<p><p>Clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostics are revolutionizing precision medicine by enabling highly sensitive detection of nucleic acid and protein biomarkers. Building on these capabilities, CRISPR-based theranostics now aim to unify real-time disease detection with targeted therapeutic interventions. However, traditional CRISPR diagnostics face several limitations, including restricted multiplexing, off-target effects, and challenges in delivery efficiency. To overcome these issues, artificial intelligence (AI) has significantly enhanced CRISPR platforms by enabling intelligent guide RNA (gRNA) design, interpretation of complex biosensor outputs, and facilitation of rapid clinical decision-making. Machine learning tools such as DeepCRISPR, Azimuth 2.0, DeepHF, and CRISPRpred support the development of highly specific gRNAs, reduce off-target events, and personalize genome-editing strategies based on individual genomic profiles. Recently, by combining CRISPR systems with nanomaterials, fluorescence-based detection, and electrochemical sensing, researchers have developed advanced biosensors capable of detecting a broad spectrum of disease biomarkers, from cancer-associated nucleic acids to viral and genetic disorders. These advances support both diagnostics and gene therapy, enabling accurate, low-cost testing at home, in point-of-care settings, and in resource-limited environments. Together, the integration of AI and CRISPR is accelerating biomarker discovery and the development of intelligent, adaptive therapeutic platforms. New point-of-care diagnostic tests (POCTs) based on CRISPR-AI are essential for early screening of high-mortality diseases, and CRISPR-based diagnostic assays have emerged as powerful, versatile alternatives to traditional nucleic acid tests, offering rapid, programmable, and portable diagnostic solutions. This review explores the evolution of CRISPR-AI theranostic systems, current and emerging POCT applications. It highlights the technological, clinical, and ethical challenges shaping their translation into next-generation precision diagnostics.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"7 1","pages":"79-120"},"PeriodicalIF":0.0,"publicationDate":"2025-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13190120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148018456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunable viscoelastic collagen/polyethylene glycol composite hydrogels modulate neural and tumor cell behavior in 3D microenvironments.","authors":"Hexu Zhang, Ziyan Chen, Runxiang Yao, Yuyun Liang, Chaoyong He, Jing Yang, Houzhi Kang, Liyang Shi","doi":"10.12336/bmt.25.00096","DOIUrl":"10.12336/bmt.25.00096","url":null,"abstract":"<p><p>Three-dimensional (3D) cell culture systems provide a more physiological environment than traditional two-dimensional cultures by better mimicking the complex interactions within the extracellular matrix (ECM). Among the key properties of the ECM, viscoelasticity is essential for regulating cell behaviors, such as proliferation, differentiation, and migration. However, many present 3D culture systems are complex and technically demanding, which limits their broad application. In this study, we developed two hydrogel systems with identical stiffness but distinct viscoelastic properties, designed to serve as ECM-based 3D culture platforms. These hydrogels were constructed through the cross-linking reaction between type I collagen and functionalized polyethylene glycol derivatives, resulting in either reversible (dynamic) or stable (static) network structures. This platform effectively simulated ECM-like mechanical cues, enabling the investigation of viscoelastic effects on both neural and cancer cell responses. Our results demonstrated that dynamic hydrogels, characterized by rapid stress relaxation, enhanced PC12 cell elongation, promoted neural stem cell differentiation, and significantly facilitated the invasiveness and tumorigenic capacity of DU145 cells <i>in vitro</i> and <i>in vivo</i>. These findings highlight the critical importance of matrix viscoelasticity in modulating cell behavior and underscore the potential of this hydrogel-based system as a versatile and accessible tool for applications in neural tissue engineering, cancer research, and mechanobiology.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 4","pages":"437-449"},"PeriodicalIF":0.0,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12852074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146108735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cosmogenic intelligence: The silent power.","authors":"Zhidao Xia","doi":"10.12336/bmt.25.00178","DOIUrl":"10.12336/bmt.25.00178","url":null,"abstract":"","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 3","pages":"230-231"},"PeriodicalIF":0.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12582085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoformulation-assisted early diagnosis of prostate cancer: Advances and perspectives.","authors":"Zhiyuan Zhou, Mingyu Chang, Jingcheng Lyu, Jianhua Zhao, Zongwei Wang, Fengbo Zhang, Yinong Niu, Boyu Yang","doi":"10.12336/bmt.24.00077","DOIUrl":"10.12336/bmt.24.00077","url":null,"abstract":"<p><p>Prostate cancer is one of the most common cancers affecting men worldwide. Owing to late diagnosis, the mortality rate associated with prostate cancer remains relatively high. Traditional diagnostic methods are, in most cases, unfriendly to patients or have diagnostic lag defects. Further diagnosis requires prostate biopsy. The most common biomarker is prostate-specific antigen, which is quantified as the content of the prostate health index to describe the risk of prostate cancer. Traditional biochemical analysis methods are costly, time-consuming, and lack specificity. They are also limited by the detection range, preventing high sensitivity. The exploration of novel biomarkers has identified several promising alternatives. The development of integrated nanomaterial technology provides a feasible potential method for the rapid, sensitive and non-invasive determination of these biological markers and assists in the optimisation of imaging diagnosis, which is expected to solve the current challenges in the diagnosis of prostate cancer. This paper reviews the advances in the diagnostic screening and imaging of prostate cancer using nanostructure-based biofunctional sensors, probes and contrast agents such as gold nanoparticles, upconversion nanoparticles, quantum dots, and magnetic nanoparticles. It also highlights the potential of emerging paradigms in nanoarchitectonics to definitive cancer diagnosis.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 3","pages":"232-249"},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12582087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"m⁶A in bone homeostasis and related diseases.","authors":"Bo Ma, Junyu Su, Yuanchao Zhu, Fengshi Zhang, Hailin Ma, Tianze Sun, Hanwen Cheng, Kangling Xu, Baixin Gu, Rou Wen, Yien Zheng, Qi Yang, Deli Wang, Fei Yu","doi":"10.12336/bmt.24.00058","DOIUrl":"10.12336/bmt.24.00058","url":null,"abstract":"<p><p>N⁶-methyladenosine RNA methylation (m⁶)A is one of the most common and widespread RNA modifications in eukaryotic cells. m⁶A plays a crucial role in the regulation of pathophysiological processes of eukaryotes. Three types of m⁶A regulators, including methyltransferases, demethylases and m⁶A-binding proteins, are involved in the reversible epigenetic modification of m⁶A. Bone is a vital organ with irreplaceable functions of movement, haematopoiesis, and protection of other organs. Its physiological homeostasis is mainly determined by the synergy of corresponding cells such as bone marrow derived stem cells, osteoblasts, and osteoclasts. Once the physiological equilibrium is broken, the bones will transform into a pathological state, resulting with diseases such as osteoporosis, osteoarthritis, rheumatoid arthritis, and osteosarcoma. Here, we review the composition of m⁶A and its regulation mechanism in bone physiology and pathology.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 3","pages":"281-293"},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12582084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering strategies and biomedical applications of bacterial extracellular vesicles.","authors":"Xuying Liang, Qianbei Li, Lei Zheng, Bo Situ","doi":"10.12336/bmt.24.00032","DOIUrl":"10.12336/bmt.24.00032","url":null,"abstract":"<p><p>Bacterial extracellular vesicles (BEVs) are emerging as promising therapeutic agents and drug delivery vehicles due to their unique properties. These nanoscale vesicles possess stable membrane structure and naturally encapsulate a variety of bioactive molecules, making them versatile tools in biomedical applications. However, clinical translation of BEVs faces challenges such as insufficient display of disease-specific antigens, excessive toxicity, and rapid clearance. Addressing these issues is crucial for the clinical translation of BEVs. In this review, we discuss recent advances in BEV engineering strategies aimed at addressing these limitations and expanding their therapeutic applications. We highlight approaches for loading exogenous cargo into BEVs, detoxification strategies, and the latest progress in the application of engineered BEVs for treating infectious diseases, cancer, and other disorders. Despite promising preclinical results, clinical translation is hindered by safety concerns, standardisation difficulties, and scalability issues. Future research should focus on optimising detoxification processes, establishing global standardisation, and improving production methods to facilitate successful clinical translation of engineered BEVs. This review provides insights into the current status and future perspectives of BEV engineering for therapeutic applications.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 3","pages":"265-280"},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12582082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of natural polymer-based transdermal drug delivery system for musculoskeletal disorders.","authors":"Yantao Zhang, Guirong Wang, Yan Zhou","doi":"10.12336/bmt.24.00045","DOIUrl":"10.12336/bmt.24.00045","url":null,"abstract":"<p><p>The transdermal drug delivery system is a highly safe and well-tolerated therapeutic approach with significant potential for treating musculoskeletal disorders. However, its clinical application is limited by the low skin permeability of many active drugs in its formulations. To overcome this challenge, advancements in skin permeation enhancement techniques are essential. Over the past decade, natural polymers have been increasingly incorporated into various nanocarriers due to their availability, biodegradability, and biocompatibility, offering new options for the effective dispersion of suspended solids. Furthermore, surface functionalisation of the numerous functional groups found in natural polymers allows them to be transformed into targeted and stimulus-responsive materials, enabling precise drug delivery to musculoskeletal tissues. This review examines the mechanisms of action of natural polymer-based transdermal drug delivery system, covering penetration enhancers, nanoparticles, microneedles, hydrogels, and nanofibres derived from chitosan, hyaluronic acid, sodium alginate, cellulose, and proteins, and their applications in treating musculoskeletal disorders. Moreover, it outlines the current challenges and prospects of polymer-based transdermal drug delivery system for localised treatment, offering insights into current therapeutic approaches and proposing new directions for advancements in this field.</p>","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"6 3","pages":"314-333"},"PeriodicalIF":0.0,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12582064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}