Biomaterials Translational最新文献_第10页

Application of stem cells in translational medicine. 干细胞在转化医学中的应用。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.12336/biomatertransl.2021.04.002

James T Triffitt, Qian Wang

引用次数: 2

Role of hypoxia inducible factor 1α in cobalt nanoparticle induced cytotoxicity of human THP-1 macrophages. 缺氧诱导因子1α在钴纳米颗粒诱导人THP-1巨噬细胞毒性中的作用。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.12336/biomatertransl.2021.02.004

Wendy Rachel Francis, Zhao Liu, Sian E Owens, Xiao Wang, Huaming Xue, Alex Lord, Venkateswarlu Kanamarlapudi, Zhidao Xia, Zx, Wrf, Zx, Wrf, Zl, Wrf, Xw, Wrf, Seo, Xw, Hx, Al, Vk, Zl, Wrf, Zl, Al, Wrf, Zl, Vk, Zx

{"title":"Role of hypoxia inducible factor 1α in cobalt nanoparticle induced cytotoxicity of human THP-1 macrophages.","authors":"Wendy Rachel Francis, Zhao Liu, Sian E Owens, Xiao Wang, Huaming Xue, Alex Lord, Venkateswarlu Kanamarlapudi, Zhidao Xia, Zx, Wrf, Zx, Wrf, Zl, Wrf, Xw, Wrf, Seo, Xw, Hx, Al, Vk, Zl, Wrf, Zl, Al, Wrf, Zl, Vk, Zx","doi":"10.12336/biomatertransl.2021.02.004","DOIUrl":"https://doi.org/10.12336/biomatertransl.2021.02.004","url":null,"abstract":"Cobalt is one of the main components of metal hip prostheses and cobalt nanoparticles (CoNPs) produced from wear cause inflammation, bone lyses and cytotoxicity at high concentrations. Cobalt ions mimic hypoxia in the presence of normal oxygen levels, and activate hypoxic signalling by stabilising hypoxia inducible transcription factor 1α (HIF1α). This study aimed to assess in vitro the functional role of HIF1α in CoNP induced cellular cytotoxicity. HIF1α, lysosomal pH, tumour necrosis factor α and interleukin 1β expression were analysed in THP-1 macrophages treated with CoNP (0, 10 and 100 μg/mL). HIF1α knock out assays were performed using small interfering RNA to assess the role of HIF1α in CoNP-induced cytotoxicity. Increasing CoNP concentration increased lysosomal activity and acidity in THP-1 macrophages. Higher doses of CoNP significantly reduced cell viability, stimulated caspase 3 activity and apoptosis. Reducing HIF1αactivity increased the pro-inflammatory activity of tumour necrosis factorαand interleukin 1β,but had no significant impact on cellular cytotoxicity. This suggests that whilst CoNP promotes cytotoxicity and cellular inflammation, the apoptotic mechanism is not dependent on HIF1α.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 2","pages":"143-150"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b1/7d/bt-02-02-143.PMC9255782.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10671579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts. 局灶黏附调节间充质干细胞和成骨细胞的成骨分化。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.12336/biomatertransl.2021.04.007

Yang Zhao, Qing Sun, Bo Huo, Yz, Bh, Yz, Bh, Yz, Yz, Bh, Sq, Yz, Bh

{"title":"Focal adhesion regulates osteogenic differentiation of mesenchymal stem cells and osteoblasts.","authors":"Yang Zhao, Qing Sun, Bo Huo, Yz, Bh, Yz, Bh, Yz, Yz, Bh, Sq, Yz, Bh","doi":"10.12336/biomatertransl.2021.04.007","DOIUrl":"https://doi.org/10.12336/biomatertransl.2021.04.007","url":null,"abstract":"Focal adhesions are large macromolecular assemblies through which cells are connected with the extracellular matrix so that extracellular signals can be transmitted inside cells. Some studies have focused on the effect of cell shape on the differentiation of stem cells, but little attention has been paid to focal adhesion. In the present study, mesenchymal stem cells (MSCs) and osteoblast-like MC3T3-E1 cells were seeded onto micropatterned substrates on which circular adhesive islands with different spacing and area were created for focal adhesion. Results showed that the patterns of focal adhesion changed cell morphology but did not affect cell survival. For MSCs cultured for 3 days, patterns with small circles and large spacing promoted osteogenesis. For MSCs cultured for 7 days, patterns with large circles and spacing enhanced osteogenesis. For MC3T3-E1 cells, the patterns of focal adhesion had no effect on cell differentiation after 3 days of culture, but patterns with small circles and spacing improved osteogenic differentiation after 7 days. Moreover, the assembly of F-actin, phosphorylation of myosin, and nuclear translocation of yes-associated proteins (YAP) were consistent with the expression of differentiation markers, indicating that the pattern of focal adhesion may affect the osteogenesis of MSCs and osteoblasts through changes in cytoskeletal tension and nuclear localisation of YAP.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 4","pages":"312-322"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8e/d0/bt-02-04-312.PMC9255797.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10671580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Engineering immune-responsive biomaterials for skin regeneration. 皮肤再生的工程免疫应答生物材料。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.3877/cma.j.issn.2096-112X.2021.01.008

Pingli Wu, Yangyang Liang, Guoming Sun, Pw, Gs, Gs, Pw, Yl, Yl

{"title":"Engineering immune-responsive biomaterials for skin regeneration.","authors":"Pingli Wu, Yangyang Liang, Guoming Sun, Pw, Gs, Gs, Pw, Yl, Yl","doi":"10.3877/cma.j.issn.2096-112X.2021.01.008","DOIUrl":"https://doi.org/10.3877/cma.j.issn.2096-112X.2021.01.008","url":null,"abstract":"The progress of biomaterials and tissue engineering has led to significant advances in wound healing, but the clinical therapy to regenerate perfect skin remains a great challenge. The implantation of biomaterial scaffolds to heal wounds inevitably leads to a host immune response. Many recent studies revealed that the immune system plays a significant role in both the healing process and the outcome. Immunomodulation or immuno-engineering has thus become a promising approach to develop pro-regenerative scaffolds for perfect skin regeneration. In this paper, we will review recent advancements in immunomodulating biomaterials in the field of skin repair and regeneration, and discuss strategies to modulate the immune response by tailoring the chemical, physical and biological properties of the biomaterials. Understanding the important role of immune responses and manipulating the inherent properties of biomaterials to regulate the immune reaction are approaches to overcome the current bottleneck of skin repair and regeneration.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 1","pages":"61-71"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4a/7b/bt-02-01-61.PMC9255827.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10671581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Human pluripotent stem cells: tools for regenerative medicine. 人类多能干细胞:再生医学的工具。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.12336/biomatertransl.2021.04.004

Peter W Andrews, Pwa

引用次数: 4

Surface topography and free energy regulate osteogenesis of stem cells: effects of shape-controlled gold nanoparticles. 表面形貌和自由能调节干细胞成骨:形状控制金纳米颗粒的影响。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.12336/biomatertransl.2021.02.006

Kamolrat Metavarayuth, Esteban Villarreal, Hui Wang, Qian Wang, Hw, Qw, Mk, Ev, Mk, Mk, Hw, Qw, Mk, Hw, Qw

{"title":"Surface topography and free energy regulate osteogenesis of stem cells: effects of shape-controlled gold nanoparticles.","authors":"Kamolrat Metavarayuth, Esteban Villarreal, Hui Wang, Qian Wang, Hw, Qw, Mk, Ev, Mk, Mk, Hw, Qw, Mk, Hw, Qw","doi":"10.12336/biomatertransl.2021.02.006","DOIUrl":"https://doi.org/10.12336/biomatertransl.2021.02.006","url":null,"abstract":"The surface free energy of a biomaterial plays an important role in the early stages of cell-biomaterial interactions, profoundly influencing protein adsorption, interfacial water accessibility, and cell attachment on the biomaterial surface. Although multiple approaches have been developed to engineer the surface free energy of biomaterials, systematically tuning their surface free energy without altering other physicochemical properties remains challenging. In this study, we constructed an array of chemically-equivalent surfaces with comparable apparent roughness through assembly of gold nanoparticles adopting various geometrically-distinct shapes but all capped with the same surface ligand, (1-hexadecyl)trimethylammonium chloride, on cell culture substrates. We found that bone marrow stem cells exhibited distinct osteogenic differentiation behaviours when interacting with different types of substrates comprising shape-controlled gold nanoparticles. Our results reveal that bone marrow stem cells are capable of sensing differences in the nanoscale topographical features, which underscores the role of the surface free energy of nanostructured biomaterials in regulating cell responses. The study was approved by Institutional Animal Care and Use Committee, School of Medicine, University of South Carolina.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 2","pages":"165-173"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8c/83/bt-02-02-165.PMC9255781.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10678428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

In silico modelling of the corrosion of biodegradable magnesium-based biomaterials: modelling approaches, validation and future perspectives. 可生物降解镁基生物材料腐蚀的硅模拟:模拟方法、验证和未来展望。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.12336/biomatertransl.2021.03.008

Aditya Joshi, George Dias, Mark P Staiger, Aj, Mps, Gd

{"title":"In silico modelling of the corrosion of biodegradable magnesium-based biomaterials: modelling approaches, validation and future perspectives.","authors":"Aditya Joshi, George Dias, Mark P Staiger, Aj, Mps, Gd","doi":"10.12336/biomatertransl.2021.03.008","DOIUrl":"https://doi.org/10.12336/biomatertransl.2021.03.008","url":null,"abstract":"Metallic biomedical implants based on magnesium, zinc and iron alloys have emerged as bioresorbable alternatives to permanent orthopaedic implants over the last two decades. The corrosion rate of biodegradable metals plays a critical role in controlling the compatibility and functionality of the device in vivo. The broader adoption of biodegradable metals in orthopaedic applications depends on developing in vitro methods that accurately predict the biodegradation behaviour in vivo. However, the physiological environment is a highly complex corrosion environment to replicate in the laboratory, making the in vitro-to-in vivo translation of results very challenging. Accordingly, the results from in vitro corrosion tests fail to provide a complete schema of the biodegradation behaviour of the metal in vivo. In silico approach based on computer simulations aim to bridge the observed differences between experiments performed in vitro and vivo. A critical review of the state-of-the-art of computational modelling techniques for predicting the corrosion behaviour of magnesium alloy as a biodegradable metal is presented.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 3","pages":"257-271"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a5/f2/bt-02-03-257.PMC9255808.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10680839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Magnesium-based materials in orthopaedics: material properties and animal models. 骨科用镁基材料:材料特性和动物模型。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.12336/biomatertransl.2021.03.004

Xirui Jing, Qiuyue Ding, Qinxue Wu, Weijie Su, Keda Yu, Yanlin Su, Bing Ye, Qing Gao, Tingfang Sun, Xiaodong Guo, Xj, Ts, Xg, Xj, Xj, Qd, Ws, Ky, Xj, Qd, Ws, Ky, Ys, By, Qw, Qg, Ts, Xj, Qd, Ws, Ky, Xj, Xj, Qd, Ws, Ky, Xj, Qd, Ws, Ky, Ys, Xg

{"title":"Magnesium-based materials in orthopaedics: material properties and animal models.","authors":"Xirui Jing, Qiuyue Ding, Qinxue Wu, Weijie Su, Keda Yu, Yanlin Su, Bing Ye, Qing Gao, Tingfang Sun, Xiaodong Guo, Xj, Ts, Xg, Xj, Xj, Qd, Ws, Ky, Xj, Qd, Ws, Ky, Ys, By, Qw, Qg, Ts, Xj, Qd, Ws, Ky, Xj, Xj, Qd, Ws, Ky, Xj, Qd, Ws, Ky, Ys, Xg","doi":"10.12336/biomatertransl.2021.03.004","DOIUrl":"https://doi.org/10.12336/biomatertransl.2021.03.004","url":null,"abstract":"As a new generation of medical metal materials, degradable magnesium-based materials have excellent mechanical properties and osteogenic promoting ability, making them promising materials for the treatment of refractory bone diseases. Animal models can be used to understand and evaluate the performance of materials in complex physiological environments, providing relevant data for preclinical evaluation of implants and laying the foundation for subsequent clinical studies. To date, many researchers have studied the biocompatibility, degradability and osteogenesis of magnesium-based materials, but there is a lack of review regarding the effects of magnesium-based materials in vivo. In view of the growing interest in these materials, this review briefly describes the properties of magnesium-based materials and focuses on the safety and efficacy of magnesium-based materials in vivo. Various animal models including rats, rabbits, dogs and pigs are covered to better understand and evaluate the progress and future of magnesium-based materials. This literature analysis reveals that the magnesium-based materials have good biocompatibility and osteogenic activity, thus causing no adverse reaction around the implants in vivo, and that they exhibit a beneficial effect in the process of bone repair. In addition, the degradation rate in vivo can also be improved by means of alloying and coating. These encouraging results show a promising future for the use of magnesium-based materials in musculoskeletal disorders.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 3","pages":"197-213"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d6/74/bt-02-03-197.PMC9255805.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10680840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Recombinant adeno-associated virus-based gene therapy combined with tissue engineering for musculoskeletal regenerative medicine. 重组腺相关病毒基因治疗联合组织工程用于肌肉骨骼再生医学。

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.3877/cma.j.issn.2096-112X.2021.01.004

Yiqing Wang, Xiangyu Chu, Bing Wang, Yw, Bw, Xc, Bw

{"title":"Recombinant adeno-associated virus-based gene therapy combined with tissue engineering for musculoskeletal regenerative medicine.","authors":"Yiqing Wang, Xiangyu Chu, Bing Wang, Yw, Bw, Xc, Bw","doi":"10.3877/cma.j.issn.2096-112X.2021.01.004","DOIUrl":"https://doi.org/10.3877/cma.j.issn.2096-112X.2021.01.004","url":null,"abstract":"Recombinant adeno-associated viral (rAAV) vector-mediated gene delivery is a novel molecular therapeutic approach for musculoskeletal disorders which achieves tissue regeneration by delivering a transgene to the impaired tissue. In recent years, substantial scientific progress in rAAV gene therapy has led to several clinical trials for human musculoskeletal diseases. Nevertheless, there are still limitations in developing an optimal gene therapy model due to the low transduction efficiency and fast degradation of the gene vectors. To overcome the challenges of rAAV gene therapy, tissue engineering combined with gene therapy has emerged as a more promising alternative. An rAAV viral vector incorporated into a biomaterial has a more controlled gene expression, lower immune response, and higher efficiency. A number of biomaterials and architectures have been combined with rAAV viral vectors, each having its own advantages and limitations. This review aims to give a broad introduction to combinatorial therapy and the recent progress this new technology has offered.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 1","pages":"19-29"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b2/88/bt-02-01-19.PMC9255831.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10678431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Physicochemical properties of respiratory droplets and their role in COVID-19 pandemics: a critical review. 呼吸道飞沫的物理化学性质及其在COVID-19大流行中的作用:一项重要综述

Biomaterials Translational Pub Date : 2021-01-01 DOI: 10.3877/cma.j.issn.2096-112X.2021.01.003

Ting Ge, Shengfeng Cheng, Tg

{"title":"Physicochemical properties of respiratory droplets and their role in COVID-19 pandemics: a critical review.","authors":"Ting Ge, Shengfeng Cheng, Tg","doi":"10.3877/cma.j.issn.2096-112X.2021.01.003","DOIUrl":"https://doi.org/10.3877/cma.j.issn.2096-112X.2021.01.003","url":null,"abstract":"The ongoing coronavirus disease 2019 (COVID-19) pandemic is a serious challenge faced by the global community. Physical scientists can help medical workers and biomedical scientists, engineers, and practitioners, who are working on the front line, to slow down and eventually contain the spread of the COVID-19 virus. This review is focused on the physicochemical characteristics, including composition, aerodynamics, and drying behavior of respiratory droplets as a complex and multicomponent soft matter system, which are the main carrier of the virus for interpersonal transmission. The distribution and dynamics of virus particles within a droplet are also discussed. Understanding the characteristics of virus-laden respiratory droplets can lead to better design of personal protective equipment, frequently touched surfaces such as door knobs and touchscreens, and filtering equipment for indoor air circulation. Such an understanding also provides the scientific basis of public policy, including social distancing rules and public hygiene guidelines, implemented by governments around the world.","PeriodicalId":58820,"journal":{"name":"Biomaterials Translational","volume":"2 1","pages":"10-18"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d8/14/bt-02-01-10.PMC9255823.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10678432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3