Future Journal of Pharmaceutical Sciences最新文献

{"title":"Antioxidant and acetylcholinesterase inhibitory activities, in silico analyses, and anti-Alzheimer’s disease potential of leaf extracts of three Nigerian endemic medicinal plants (Spondias mombin, Carica papaya and Kalanchoe crenata)","authors":"Aanuoluwapo Ruth Adetuyi,&nbsp;Michael E. Ayenero,&nbsp;Mary T. Olaleye,&nbsp;Afolabi A. Akindahunsi,&nbsp;Afolabi C. Akinmoladun","doi":"10.1186/s43094-023-00578-x","DOIUrl":"10.1186/s43094-023-00578-x","url":null,"abstract":"<div><h3>Background</h3><p>The evaluation of the correlations between antioxidant and anti-acetylcholinesterase activities of methanol leaf extracts of three Nigerian endemic plants, <i>Spondias mombin</i>, <i>Carica papaya</i> and <i>Kalanchoe crenata</i>, was carried out. Their constituent phytochemicals were identified by HPLC–DAD fingerprinting. The antioxidant activity as typified by 2,2-diphenyl-1-picrylhydrazyl (DPPH^·), 2,2′-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid (ABTS^·+) and nitric oxide (NO) scavenging activities were evaluated. The acetylcholinesterase (AChE) inhibitory activity of the extracts was also determined.</p><h3>Results</h3><p>The extracts contained appreciable amounts of the flavonoids, quercetin and kaempferol<i>. </i>The extracts of <i>Spondias mombin</i>, <i>Carica papaya</i> and <i>Kalanchoe crenata</i> showed concentration-dependent inhibitory activities against DPPH^· and ABTS^·+ with IC₅₀ of 43.29 ± 0.443 µg/mL, 59.27 ± 0.644 µg/mL and 80.20 ± 0.414 µg/mL; 25.43 ± 0.325 (µg/mL), 39.84 ± 0.163 µg/mL and 59.02 ± 0.376 (µg/mL), respectively. The IC₅₀ for the NO scavenging activities of the <i>Spondias mombin</i>, <i>Carica papaya</i> and <i>Kalanchoe crenata</i> extracts were 41.99 ± 0.217 µg/mL, 50.44 ± 0.281 µg/mL and 60.12 ± 0.512 µg/mL, respectively. The IC₅₀ for the inhibitory effects on AChE was 53.24 ± 0.327 µg/mL, 60.95 ± 0.290 µg/m and 70.5 ± 0.426 µg/mL, respectively. The effectiveness of the plant in all the experimental tests was in the following order: <i>S. mombin</i> &gt; <i>C. papaya</i> &gt; <i>K. crenata.</i> The total flavonoid and total phenolic contents have extremely significant positive correlations with the antioxidant activities and AChE inhibitory activity. The correlation coefficients (<i>r</i>²) of DPPH scavenging activity and NO scavenging activity with the AChE inhibitory activity were 0.8295 µg/mL and 0.7337 µg/mL, respectively (<i>P</i> &lt; 0.0001). The molecular docking and pharmacokinetic analyses on some constituent phytochemicals showed that quercetin, kaempferol, ferulic acid, leucocyanidin, gallic acid and isorhamnetin fulfilled the requirements for an anti-Alzheimer drug.</p><h3>Conclusions</h3><p>The results suggest that the plant species provide a significant source of secondary metabolites that can act as natural antioxidants and acetylcholinesterase inhibitors, which will be helpful in the treatment of Alzheimer’s disease.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00578-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139399841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the power of precision medicine: exploring the role of biomarkers in cancer management","authors":"Dipak D. Gadade,&nbsp;Hariom Jha,&nbsp;Chetan Kumar,&nbsp;Farzan Khan","doi":"10.1186/s43094-023-00573-2","DOIUrl":"10.1186/s43094-023-00573-2","url":null,"abstract":"<div><h3>Background</h3><p>Personalized or Precision medicine (PM) is a promising approach for the cancer treatment that tailors treatment to a patient's characteristics. Biomarkers are crucial for identifying the patients who are expected to derive greatest advantage from targeted therapy.</p><h3>Main body</h3><p>Here, various biomarkers, including genetic, epigenetic, protein, and metabolites, and their clinical significance, are discussed. The review provides insights into the use of biomarkers and their clinical significance in cancer treatment. There are several hurdles in use of PM in oncology, such as the complexity of tumor biology and heterogeneity, limited availability of biomarkers, high cost of targeted therapies, resistance to targeted therapies, and ethical and social issues.</p><h3>Conclusion</h3><p>The biomarkers play a crucial diagnostic role in the treatment of cancer. The review also acknowledges the challenges and limitations of personalized medicine which, if resolved, can be helpful in the management of cancer.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00573-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139109761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocrystals: an emerging paradigm for cancer therapeutics","authors":"Ayush Patel,&nbsp;Krishi Patel,&nbsp;Viral Patel,&nbsp;Mithun Singh Rajput,&nbsp;Ravish Patel,&nbsp;Amarjitsing Rajput","doi":"10.1186/s43094-024-00579-4","DOIUrl":"10.1186/s43094-024-00579-4","url":null,"abstract":"<div><h3>Background</h3><p>Medical fraternity are continuously pitching toward the development of novel mechanisms to combat the menace of cancer and to enhance the efficacy of prevailing molecules. During the drug development phase, majority of new molecular entity pose a threat due to hydrophobic nature, that compromises its bioavailability upon administration. These suboptimal accumulation and low drug loading hampers the clinical translation in cancer therapy.</p><h3>Main body of abstract</h3><p>Nanotechnology with valuable advantages create possibilities to accelerate the efficacy of treatment. Compared to matrix-based formulations, drug nanocrystals (NCs) with smaller size, high drug loading, high active targeting, extended circulation, great structural stability, tailored dissolution, and being carrier free have sparked a lot of interest in drug delivery. Many hydrophobic drugs were explored as drug NCs such as—doxorubicin, paclitaxel, campothecin and so on. However, premature leakage and clearance by mononuclear phagocytosis system lead to some great obstacles in the clinical applications of drug NCs.</p><h3>Conclusion</h3><p>In the recent years, strategies leading to surface modification are applied to improve uncontrolled drug release and targeting efficiency to tumor cells. The current review sheds light on various properties of drug nanocrystals, brief insights on its fabricating techniques, approaches for tumor targeting with NCs, and their applications in cancer imaging and therapeutics.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00579-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139103847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating in silico molecular docking, ADMET analysis of C.verticillata with diabetic markers and in vitro anti-inflammatory activity","authors":"Maheswari A.,&nbsp;Salamun DE","doi":"10.1186/s43094-023-00576-z","DOIUrl":"10.1186/s43094-023-00576-z","url":null,"abstract":"<div><h3>Background</h3><p>Over the past decade, various research studies have proved the interconnection between the inflammatory pathways and diabetes complication in clinical condition. The present study evaluated the anti-inflammatory and antioxidant activity. Further, the sample was tested for its pharmacokinetics properties and the best compounds were docked with the diabetic markers (DPP IV (PDB-ID: IJ2E) and SGLT2 (PDB-ID: 7VSI))<i>.</i></p><h3>Results</h3><p><i>C.verticillata</i> showed a good hydrogen peroxide (78.3 ± 0.34%, IC₅₀ = 287.81 µg/ml) and superoxide scavenging activity (52.7 ± 1.26%, IC₅₀ = 796.15 µg/ml). In addition, the sample was checked for its anti-inflammatory activity with protein denaturation (57.4 ± 0.19%, IC₅₀ = 471.5 µg/ml) and proteinase inhibition assay (68.3 ± 0.48%, IC₅₀ = 213.42 µg/ml). Further, the bioactive compounds detected from HPLC-ESI-MS/MS analyzed sample were checked for its drug likeliness by checking its ADME properties and toxicological parameters. It has been observed that except Loliolide, all the other compounds have followed the physicochemical parameters and proved to exhibit drug likeliness characteristics. The bioactive compounds that follow the Lipinski’s rule were taken further for in silico molecular docking analysis with the diabetic protein markers (DPP IV and SGLT2). Docking results revealed that Pyro pheophorbide a with DPP IV and Dihydromonacolin L acid with SGLT2 have recorded a maximum docking score of (− 9.4 kcal/mol) and (− 9.2 kcal/mol), respectively.</p><h3>Conclusion</h3><p>The observed results suggest that the identified and selected bioactive compounds from <i>C.verticillata</i> can be considered as a potential target molecule for the management of oxidative stress induced diabetic condition. Furthermore, the study also provides an insight on the effectiveness of the compounds on reducing the inflammation as well.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00576-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139103850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifaceted applications of micro/nanorobots in pharmaceutical drug delivery systems: a comprehensive review","authors":"Tanisha Das,&nbsp;Shirin Sultana","doi":"10.1186/s43094-023-00577-y","DOIUrl":"10.1186/s43094-023-00577-y","url":null,"abstract":"<div><h3>Background</h3><p>Drug delivery systems (DDSs) encompass a wide range of methods, including oral, injectable, and topical routes of administration, all tailored to meet specific patient needs. Micro and nanorobots, equipped with pioneering propulsion mechanisms that convert external energy sources into precise movements, have revolutionized drug delivery. This cutting-edge technology ensures highly efficient drug delivery, particularly when targeting specific targets within intricate physiological environments. In contrast to traditional drug delivery approaches that rely on bloodstream circulation, engineered micro/nanorobots have autonomous mobility, enabling drug delivery to previously unreachable areas.</p><h3>Main body of the abstract</h3><p>Integrating micro/nanorobots into drug delivery raises vital safety and biocompatibility issues. These encompass material selection, degradation in-vivo, overcoming biological barriers, controlled movement, external interference, immune response, chemical reactions, systemic effects, long-term impact, and real-time monitoring. While micro/nanorobots hold immense transformative potential, they confront significant hurdles in their journey toward practical applications. Chief among these challenges are concerns regarding biocompatibility, ensuring that these tiny devices do not trigger adverse reactions. Long-term safety remains a critical issue, as understanding the effects of prolonged exposure and potential accumulations within the body and navigating complex biological environments with precision is another obstacle.</p><h3>Short conclusion</h3><p>The paper summarizes how to explore the various ways in which micro/nanorobots can be employed to enhance drug delivery, including their precision, targeting capabilities, and adaptability to different physiological conditions. Additionally, the review seeks to highlight the transformative potential of these technologies and their impact on the pharmaceutical industry.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00577-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139083726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioanalytical method development and validation for the simultaneous estimation of Olanzapine and Samidorphan in rabbit plasma by using HPLC–MS/MS and application to pharmacokinetic study","authors":"Rambabu Kantipudi,&nbsp;Sugandha Kumar Pavuluri","doi":"10.1186/s43094-023-00570-5","DOIUrl":"10.1186/s43094-023-00570-5","url":null,"abstract":"<div><h3>Background</h3><p>Samidorphan is an opioid antagonist while Olanzapine is an effective medication for schizophrenia and bipolar disorder. A unique and accurate MS/HPLC approach due to simultaneous measurement of Olanzapine and Samidorphan is, therefore, more urgently required. Simultaneous quantification of Olanzapine and Samidorphan in rabbit plasma using HPLC-MS. Using a buffer composed of 1 mL of formic acid in 1 L of water and a mixture of two components, buffer and acetonitrile in a ratio of 50:50 and a flow rate of 1 mL/min at room temperature, we separated compounds on an Inertsil ODS column (250 × 4.6 mm, 5 m).</p><h3>Results</h3><p>Analysis was performed within 8 min over a satisfactory linear concentration range of 2–40 ng/mL for Olanzapine (<i>r</i>² = 0.99901 0.024) and 2–40 ng/mL for Samidorphan (<i>r</i>² = 0.99927 0.012). The matrix effect recoveries of Olanzapine and Samidorphan at various QC concentration levels were 104.5, 100.51% and 110.36, 99.25%, respectively. The precision and recovery study outcomes fall within the acceptable range. An electrospray ionization source was used to analysis of Olanzapine and Samidorphan at m/z 313.40 → 192.54, m/z 371.45 → 220.61 for Olanzapine and Samidorphan, m/z 316.40 → 237.58, m/z 374.41 → 223.61 for D₃ Olanzapine and D₃ Samidorphan that were ion pairs of mass analysis.</p><h3>Conclusions</h3><p>Liquid–liquid extraction was used to remove Olanzapine (0.17 mg/kg) and its reference standard (D₃-Olanzapine) from rabbit plasma. Both the active compound Samidorphan (0.17 mg/kg) and its reference, D₃-samidorphan, were isolated from rabbit plasma. We conducted stability studies to ensure that the medications would remain stable in accordance with USFDA regulations.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00570-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139079127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-calcium incorporated piscean collagen scaffolds: potential wound dressing material","authors":"Chaitra Shree TJ,&nbsp;Sindhu Abraham,&nbsp;Sharon Furtado,&nbsp;Darshan Ramesh,&nbsp;Kesha Desai,&nbsp;Bharath Srinivasan","doi":"10.1186/s43094-023-00566-1","DOIUrl":"10.1186/s43094-023-00566-1","url":null,"abstract":"<div><h3>Background</h3><p>Collagen proteins extracted from piscean sources are alternatives to bovine and porcine collagen because of their abundance, low price, and skin compatibility and are being explored as suitable wound dressing materials. Intracellular calcium ions are crucial for wound healing, and studies have shown that calcium ion supplementation via an external medium is equally beneficial for speedy recovery. This study explores the wound healing potential of dressing materials that encompass the benefits of nano-calcium and piscean collagen. Nano-calcium sulphate (NCS)-integrated scaffolds were prepared with 100 ppm of NCS and varying concentrations of piscean collagen and HPMC E15 LV. The thickness, tensile strength, folding endurance, pH, expansion profile, and moisture vapour transmission properties of the scaffolds were determined. An in vitro scratch assay and an excision rat wound model were employed to evaluate the wound healing properties of the scaffolds.</p><h3>Results</h3><p>The NCS particles had a mean particle size of 220.7 nm. The scaffolds demonstrated an acceptable thickness, mechanical strength, and flexibility. The scratch assay results revealed that at the end of 24 h of the study, there was an increased wound closure rate with collagen scaffolds in contrast to the control group. In the vivo wound healing studies, formulation CS6 showed 100.0% healing on day 12 as compared to other formulations.</p><h3>Conclusions</h3><p>Wounds treated with scaffolds contracted faster than those treated with a commercial collagen dressing and the control group. The current study thus demonstrates the wound healing ability of nano-calcium sulphate-incorporated piscean collagen scaffolds.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"9 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00566-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138952373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoemulsion drug delivery system loaded with imiquimod: a QbD-based strategy for augmenting anti-cancer effects","authors":"Shital Tanaji Jadhav,&nbsp;Vijay Rajaram Salunkhe,&nbsp;Somnath Devidas Bhinge","doi":"10.1186/s43094-023-00568-z","DOIUrl":"10.1186/s43094-023-00568-z","url":null,"abstract":"<div><h3>Background</h3><p>Skin cancer is becoming a public health concern due to increased exposure to environmental pollutants and UV rays, among other factors. In India, skin neoplasms constitute 2–3% of all human cancer cases, whereas in the USA, 2–3 million cases of non-melanoma skin cancer are reported annually. Various drugs are available in the market for treating skin cancer. Imiquimod (IMQ) is one such drug approved by the USFDA for managing basal cell malignancy, external genital warts, and actinic keratosis. The conventional dosage form of IMQ cream has several side effects that can lead to therapy interruption. Therefore, the present work aims to develop an IMQ nanoemulsion with improved solubility, in vitro drug release and stability. Nanoemulsion was formulated using oleic acid/rose oil, with polysorbate 20/propylene glycol selected as the oil phase and Smix, respectively. Optimization carried out using a 3² factorial design with the aid of a quadratic model. Characterization was conducted for parameters, namely viscosity, pH, drug content, globule size, zeta potential and entrapment efficiency. Thermodynamic stability studies were conducted to assess the stability of the formulation. Furthermore, the optimized system was subjected to TEM analysis, in vitro drug release and in vitro cytotoxicity assay (MTT assay).</p><h3>Results</h3><p>Nanoemulsions were found to be in the size range of 152.80–470.13 nm and exhibited a spherical shape. Zeta potential values ranged from − 28.93 to − 58.48 mV. DSC measurements indicated the complete solubilization of IMQ in the nanoemulsion system. The optimized formulation F1 displayed the following characteristics: a globule size &lt; 200 nm, a zeta potential &gt; − 55 mV, a polydispersity index &lt; 0.2, % drug content of 102.89 ± 1.06, % entrapment efficiency of 97.59 ± 0.24, a pH of 4.77 ± 0.06, and a viscosity of 4.06 ± 0.06 poise. In vitro IMQ release studies of nanoemulsion and commercial cream showed approximately 70% and 34% drug release, respectively, at the end of 8 h. Moreover, the in vitro cytotoxicity assay depicted that <i>F</i>1 exhibited greater cytotoxic potential compared to the commercial formulation against the A431 cell line.</p><h3>Conclusion</h3><p>The present investigation showed a significant improvement in in vitro drug release of the BCS class IV drug IMQ and enhanced cytotoxic activity against cancerous cells. IMQ-loaded nanoemulsion represents a promising vehicle for delivering treatment to the skin for treating skin cancer.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"9 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00568-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138819371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of undescribed flavonoid from Abrus precatorius L. based on HPTLC-DPPH bioautography and its cytotoxicity evaluation","authors":"Hafsa I. Ansari,&nbsp;Ranjitsinh C. Dabhi,&nbsp;Pooja G. Trivedi,&nbsp;Milan S. Thakar,&nbsp;Jayesh J. Maru,&nbsp;Gaurang M. Sindhav","doi":"10.1186/s43094-023-00571-4","DOIUrl":"10.1186/s43094-023-00571-4","url":null,"abstract":"<div><h3>Background</h3><p>Naturally derived compounds play a tremendous role as a drug as well as lead structure for the development of APIs. Therefore, isolation and characterization of compounds from nature are needed to alleviate life-threatening diseases. <i>A. precatorius</i> L. belongs to the family Leguminosae and is valued for its medicinal properties. Therefore, in this study, efforts are being made to isolate bioactive entity based on HPTLC-DPPH bioautography from APHA extract. Among all the separated compounds on TLC plate, the one (APSP-3) at <i>R</i>_f = 0.67 showed significant antioxidant activity, and hence, APSP-3 was further subjected to isolation, purification, and structural characterization using diverse analytical modus operandi such as 1D and 2D NMR, FTIR, HPLC–MS/MS, and elemental analysis. In addition, antioxidant and cytotoxicity evaluation of APHA extract and APSP-3 was pursued by standard DPPH and colorimetric MTT assays, respectively.</p><h3>Results</h3><p>Antioxidative isolated compound APSP-3 was scrutinized based on HPTLC-DPPH bioautography. The APSP-3 was found novel and spectroscopic data revealed the plausible structure; 7-hydroxy-3,5-dimethoxy-2-(4-((3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2<i>H</i>-pyran-2-yl)oxy) phenyl)-4<i>H</i>-chromen-4-one. Moreover, APSP-3 ascribed higher free radical scavenging activity with IC₅₀ = 38.70 ± 3.5 µg/mL than standard ascorbic acid (75.19 ± 1.5 µg/mL). Cytotoxicity evaluation of APHA extract exhibited IC₅₀ value 122.09 µg/mL for HepG2, 122.61 µg/mL for MCF-7, and 48.08 µg/mL for HCT116 cell lines, while APSP-3 displayed IC₅₀ values 96.75 for HepG2, 61.67 for MCF-7, and 47.61 µg/mL for HCT116 cell lines.</p><h3>Conclusions</h3><p>In a nutshell, HPTLC-directed bioautography leads to the capturing of new flavonoid entity having antioxidant potency from APHA extract. The IC₅₀ values obtained from cytotoxicity establish a dose–response relationship helping to determine the concentration at which a substance begins to exhibit toxic effects. This fundamental information is crucial for establishing safe dosage level in medical and pharmaceutical applications. Further, research engrossed in assessing other bioactivities involving in silico and in vivo studies obliged to offer a promising and secure portrayal for clinical implications.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"9 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00571-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138741409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of telmisartan and related sartans with the programmed cell death-ligand 1 (PD-L1) protein dimer: a molecular docking analysis","authors":"Gérard Vergoten,&nbsp;Christian Bailly","doi":"10.1186/s43094-023-00574-1","DOIUrl":"10.1186/s43094-023-00574-1","url":null,"abstract":"<div><h3>Background</h3><p>Telmisartan (TLT) is a prototypic angiotensin receptor blocker largely used to treat hypertension worldwide. In addition to its cardioprotective effects, TLT presents pleiotropic activities and notably displays noticeable anti-inflammatory and antitumor effects. The repression of the programmed cell death-1 (PD-1)/programmed death-ligand 1 (PD-L1) immune checkpoint may be implicated antitumor action of TLT, as it is the case with many other compounds equipped with a biphenyl moiety. We have used molecular modeling to compare the interaction of TLT and derivatives with the PD-L1 dimer protein.</p><h3>Results</h3><p>Two molecules, TLT-dimer and TLT-acylglucuronide, were found to form more stable complexes with PD-L1 than TLT itself. In parallel, the docking analysis performed with a series of 12 sartans led to the identification of Olmesartan as a potential PD-L1 binder. The stacked biphenyl unit of Olmesartan positions the molecule along the groove delimited by the two protein monomers. The flanking tetrazole and imidazole moieties, on each side of the biphenyl unit of Olmesartan, contribute favorably to the protein interaction via specific hydrogen bonding interactions.</p><h3>Conclusions</h3><p>The computational analysis suggests a possible binding of Olmesartan to PD-L1 dimer and thus offers novel perspectives for the design of small molecules capable of interrupting the PD-1/PD-L1 immune checkpoint. Experimental studies are warranted to validate the hypothesis.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"9 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-023-00574-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138713674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}