Future Journal of Pharmaceutical Sciences最新文献

{"title":"Synthesis and anti-microbial evaluation with in silico studies of novel 2-aminothiazole benzohydrazide derivatives","authors":"S. Amrutha,&nbsp;Paramita Das,&nbsp;Anjali Nayak,&nbsp;Supratip Laha,&nbsp;Sharmina Begum,&nbsp;Sakshi Bhardwaj","doi":"10.1186/s43094-024-00759-2","DOIUrl":"10.1186/s43094-024-00759-2","url":null,"abstract":"<div><h3>Background</h3><p>The development of novel anti-microbial drugs for multidrug-resistant (MDR) is a significant challenge. This study aimed to synthesize various derivatives of (Z)-4-(2-aminothiazol-5-yl)-N-benzohydrazide (DT01-DT10) that are effective against a wide variety of anti-bacterial and antifungal pathogens.</p><h3>Results</h3><p>The binding energy of the compounds ranged from − 9.0 to − 10.0 kcal/mol. Molecular simulations produced a major result in improving the representation of the real biological conditions with an average RMSD of 0.110 nm. The derivatives DT03, DT04, and DT06 showed overall good anti-microbial activity at lower concentrations of 1.8 µg/ml. Compound DT03 showed significant activity against Gram-positive, Gram-negative bacteria and fungal strains, with inhibition zone diameters of 17, 19 and 16 mm, respectively. Compound DT04 showed promising anti-bacterial effects against <i>S.mutans</i> and <i>C.albicans</i>, with inhibition zone diameters of 18 and 17 mm, and moderate activity against <i>B. cereus</i>. Compound DT06 showed enhanced activity against <i>P.aeruginosa</i>.</p><h3>Conclusion</h3><p>The derivative 4-(2-amino-1,3-thiazol-5-yl)-N′-(Z)-(2-nitrophenyl) methylidene benzohydrazide (DT06), which contained a nitro group displayed potent activity at 1.8 µg/ml with an IC₅₀ of 50.31 and a selectivity index of 61.33.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00759-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic nanotransferosomal vesicular system of Clerodendrum serratum L. for skin cancer therapy: formulation, characterization, and efficacy evaluation","authors":"Somnath Devidas Bhinge,&nbsp;Sayali Jadhav,&nbsp;Pranali Lade,&nbsp;Mangesh Anil Bhutkar,&nbsp;Shailendra Gurav,&nbsp;Namdeo Jadhav,&nbsp;Abhinandan Patil,&nbsp;Neeraj Upmanyu","doi":"10.1186/s43094-024-00755-6","DOIUrl":"10.1186/s43094-024-00755-6","url":null,"abstract":"<div><h3>Background</h3><p>The pivotal intent of this study was to investigate the medicinal potential of synthesized nanotransferosomes utilizing <i>Clerodendrum serratum</i> L. hydroalcoholic extract (CS-NTs). Three batches, CS-NTs 25, CS-NTs 50, and CS-NTs 100, were formulated using the thin-film hydration approach with varying concentrations of phospholipids (soya phosphatidylcholine) at 25, 50, and 100 mg, respectively, to evaluate their efficacy against skin cancer cell lines. Several analytical approaches, such as UV/Vis spectroscopy, XRD spectra, FTIR, TEM, SEM, particle size distribution, and zeta potential analysis, were employed to assess the synthesized CS-NTs.</p><h3>Results</h3><p>Experimental results revealed that CS-NTs exhibited a circular shape, with average vesicle sizes approximately measured at 463.6 ± 100.5 nm, 265.5 ± 61.6 nm, and 409.6 ± 106.2 nm for CS-NTs 25, CS-NTs 50, and CS-NTs 100, respectively. Notably, biologically synthesized CS-NTs 25 offer potential anticancer benefits against B10F16 (skin cancer cell line) cells, exhibiting no signs of adverse effects. The heightened biological potential of CS-NTs can be ascribed to their unique characteristics at the nanoscale, characterized by an average vesicle size and lower polydispersity value, which facilitate augmented responsiveness and interactions with biological systems.</p><h3>Conclusion</h3><p>CS-NTs 25 formulated through the biogenic process using <i>C. serratum</i> hydroalcoholic plant root extract hold significant potential for anticancer activity.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00755-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms and enhanced functions of Hibiscus sabdariffa L—nanoliposomes as an emerging therapeutic strategy in UV and galactosamine skin aging-induced model","authors":"Eptehal Nassar,&nbsp;Mai Rady,&nbsp;Heba Handousa","doi":"10.1186/s43094-024-00756-5","DOIUrl":"10.1186/s43094-024-00756-5","url":null,"abstract":"<div><h3>Background</h3><p>Skin aging is a multifactorial disorder that occurs due to extrinsic and intrinsic factors, where a decrease in natural antioxidant defenses and an imbalance between molecular biomarkers occur. The current study aims to develop nanoliposomes for the dermal delivery of <i>Hs</i> and to investigate their effects on skin biomarkers and skin aging.</p><h3>Methods</h3><p>Chemical profiling performed via high-performance liquid chromatography (HPLC)/ESI‒PDA‒MS revealed enrichment in phenolic metabolite contents. <i>Hs-</i>nanolopeosomes were characterized for their mean size, encapsulation efficiency of <i>Hs</i> and ability to penetrate the skin via confocal microscopy. An aged rat model generated via UV and galactosamine injection was evaluated for reduced glutathione (GSH) and malondialdehyde (MDA) levels, in addition to the levels of collagenase and elastase enzymes in the different study groups, which included a healthy control group, an aged group, a prophylactic group, an aged group treated with <i>Hs-</i>nanoliposomes, and a green tea extract-treated group (positive control). Moreover, the Bcl-2/Bax proteins were determined via ELISA, and MMP-1, MMP-2, MMP-9, and TIMP-1 expression was determined via RT‒qPCR in the study groups.</p><h3>Results</h3><p><i>Hs-</i>nanoliposomes (~ 400 nm) proved deep skin localization in confocal images. Compared with the aged group and the green tea extract-treated group, the <i>Hs</i>-liposome-treated group presented elevated reduced glutathione and decreased malondialdehyde levels and inhibited collagenase and elastase enzymes. This treatment also decreased the Bcl-2/Bax ratio and downregulated the expression of MMP-1, MMP-2, and MMP-9. However, upregulation of TIMP-1 expression was detected. The outcomes were confirmed by histopathological assays, which revealed reduced saging and collagen damage in the <i>Hs</i>-nanolipid-treated group.</p><h3>Conclusion</h3><p>The present study proposed a potential antiaging nanobased formulation that can deliver <i>Hs</i> extract deep in the dermis layer to prevent the oxidative stress that leads to aging.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00756-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142938677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the therapeutic potential of insect-derived natural products for drug discovery","authors":"Jasha Momo H. Anal,&nbsp;Lobeno Mozhui,&nbsp;Samuel Lalthazuala Rokhum","doi":"10.1186/s43094-024-00758-3","DOIUrl":"10.1186/s43094-024-00758-3","url":null,"abstract":"<div><h3>Background</h3><p>The recent FDA-approved Ycanth (cantharidin) for treating <i>Molluscum contagiosum</i>, a viral skin infection, was first reported from blister beetles. Medicinal insects are reservoirs for exploring bioactive molecules, which have various benefits. Their use in traditional medicinal practices explains why uncovering new chemical substances is worthwhile.</p><h3>Main body of the abstract</h3><p>Insect-derived natural products with diverse and unique structures are significant for drug discovery and development potential. Various natural products are reported from insect sources; in this context, it also emphasizes the importance of active global participation among researchers, as it offers significant potential for developing a sustainable approach to why this should not remain untouched for ever-increasing unmet challenges.</p><h3>Conclusion</h3><p>Hence, practitioners in natural product chemistry and allied disciplines have a role in understating the enormous potential of discovering bioactive metabolites for their medicinal value to human health.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00758-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging computational approaches in identifying novel HER-2 + breast cancer potential therapeutics: integrating virtual screening and molecular dynamics simulation","authors":"Olawale Quadri Bolaji,&nbsp;Temitope Isaac Adelusi,&nbsp;Taiwo Ooreoluwa Ojo,&nbsp;Ibrahim Damilare Boyenle,&nbsp;Abdul-Quddus Kehinde Oyedele,&nbsp;Taiwo Temitope Ogunjobi,&nbsp;Adegboye Oyewole Oyaronbi,&nbsp;Sukurat Oluwatoyin Ayoola,&nbsp;Abdeen Tunde Ogunlana","doi":"10.1186/s43094-024-00748-5","DOIUrl":"10.1186/s43094-024-00748-5","url":null,"abstract":"<div><h3>Background</h3><p>Breast cancer, particularly the human epidermal growth factor receptor 2 positive subtype, presents a significant global health challenge due to its high prevalence and mortality rates. This study delves into the molecular intricacies of HER-2 positive breast cancer, with an emphasis on the role of the HER-2 oncoprotein and its associated signaling pathways in cell growth, differentiation, and survival. In our pursuit of overcoming the limitations of one of the leading therapeutic options, Lapatinib, such as its inhibition of hERG, we embarked on a comprehensive research journey.</p><h3>Result</h3><p>This study involved dual-stage molecular docking, initially with a library of PubChem-curated compounds, revealing Compound 90196902 as the best of the set. This was followed by the docking of DataWarrior-generated structural analogs of Compound 90196902, using various docking protocols such as standard precision, extra precision, and induced fit docking. Through this rigorous screening protocol, three promising drug candidates (Compound_56, Compound_81, and Compound_339) were identified, showing excellent interaction with the target. Additionally, binding free energy calculations, ADME and toxicity profiling, and molecular dynamics simulations presented these compounds as lead-like.</p><h3>Conclusion</h3><p>Compound_56 showed the most promising pharmacodynamic and pharmacokinetic properties, coupled with substantial structural stability. While immensely promising, further optimization and pre-clinical investigation are imperative to validate this compound as a viable alternative to existing therapies for HER-2 positive breast cancer.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00748-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Augmenting the safety and therapeutic efficacy of doxycycline and rifampicin-loaded solid lipid nanoparticles against Brucella abortus using murine model","authors":"K. M. Himani,&nbsp;Subbaiyan Anbazhagan,&nbsp;Lakshmi Prakasan,&nbsp;Prasad Thomas,&nbsp;Akhilesh Kumar,&nbsp;Krishnaswamy Narayanan,&nbsp;Praveen Singh,&nbsp;Pallab Chaudhuri","doi":"10.1186/s43094-024-00753-8","DOIUrl":"10.1186/s43094-024-00753-8","url":null,"abstract":"<div><h3>Background</h3><p>Prolonged therapy, hepatic damage, and the development of drug resistance are the limitations of doxycycline and rifampicin in the treatment of brucellosis. This study was designed to assess the effect of solid lipid nanoparticles (SLNs) encapsulation of these antimicrobials against brucella. SLNs were prepared by a double emulsion method and characterized for entrapment efficiency, particle size distribution, drug release, infrared spectroscopy, differential scanning calorimetry, and scanning electron microscopy. The encapsulated formulations were tested for stability, safety, and in vitro antimicrobial assay on brucella and <i>brucella-infected</i> mice models.</p><h3>Results</h3><p>The particle size, polydispersity index and zeta potential of doxycycline encapsulated SLN size were 443.6 ± 3.03 nm, 0.858 ± 0.00, and + 26.5 ± 1.87 mV, respectively. The rifampicin SLN showed particle size, polydispersity index, and zeta potential of 348.7 ± 3.35 nm, 0.234 ± 0.00, and + 22.9 ± 1.59 mV. The entrapment efficiency of doxycycline and rifampicin encapsulated SLN was 98% and 96%, respectively. The drug-loaded SLN retained the biophysical properties, drug loading, and entrapment efficiency after storage at 4 °C as well as 25 °C for 6 months. The Fourier transform infrared spectroscopy depicted effective encapsulation and the Differential scanning colorimetry thermogram revealed a negative interaction of drug and polymer. Drug release parameters were fitted well with the Korsmeyer–Peppas and Hixson–Crowell model (<i>R</i>2 ≥ .09). The MIC findings showed a reduction in MIC values of SLN-encapsulated rifampicin; whereas, doxycycline did not show any changes. The MBC value for free and SLN-encapsulated doxycycline and rifampicin did not show changes. SLN encapsulation did not show any cytotoxicity on murine macrophage cell line RAW 264.76 for both drugs. Doxycycline and rifampicin significantly reduced the CFU of <i>B. abortus</i> compared to the free drug (<i>P</i> &gt; 0.05), resulting in less damage to the liver and kidneys.</p><h3>Conclusions</h3><p>The present study concludes that SLN encapsulation of doxycycline and rifampicin would be safe and effective for the prolonged treatment regimen against Brucella infection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00753-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repurposing dapagliflozin for Alzheimer's disease: a mechanistic exploration","authors":"Marwa M. Saeed","doi":"10.1186/s43094-024-00751-w","DOIUrl":"10.1186/s43094-024-00751-w","url":null,"abstract":"<div><h3>Background</h3><p>Several researches describe Alzheimer’s disease (AD) as Type-III diabetes mellitus due to shared pathophysiological mechanisms between AD and DM and the fact that one disease can increase the incidence of the other. Therefore, keeping glucose level under control protects the brain from its harmful effects and delays the incidence of AD in susceptible individuals by using anti-diabetic agents. Several anti-diabetic classes were explored for their protective effect against AD, among them that attracted more attention was sodium–glucose cotransporter inhibitor dapagliflozin (DAPA).</p><h3>Main body of the abstract</h3><p>This review aims at illustrating various protective mechanisms that DAPA proved to exert on cognition and memory. DAPA showed promising results by its influence on behavioral parameters highlighted enhancement of both spatial and non-spatial learning and memory, in addition to ameliorating associated anxiety by its effect on various neurotransmitters. DAPA succeeded in promoting neurogenesis, synaptic plasticity, and synaptic density, and managed to demonstrate anti-inflammatory, antioxidant, and antiapoptotic properties. Moreover, DAPA enhanced the activity of mitochondria and promoted autophagy, in addition to its impact on the PI3K/AKT/mTOR and Wnt/β-catenin signaling pathways.</p><h3>Short conclusion</h3><p>DAPA showed promising results in different AD models to enhance memory and improve cognitive deficits.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00751-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142844790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive phytochemicals from leaf extract of Erlangea tomentosa S. Moore (Bothriocline longipes (Oliv. & Hiern.) used to treat bacterial infections in Uganda","authors":"Jane Namukobe,&nbsp;Yeremiah Ekyibetenga,&nbsp;Adebola Omowunmi Oyedeji,&nbsp;Robert Byamukama,&nbsp;Mathias Heydenrech","doi":"10.1186/s43094-024-00749-4","DOIUrl":"10.1186/s43094-024-00749-4","url":null,"abstract":"<div><h3>Background</h3><p>The leaves of <i>Erlangea tomentosa</i> (ET) are used to treat several bacterial infections like respiratory tract infections, skin infections, diarrhea and gastrointestinal disorders. However, there is limited information about the compounds responsible for its bacterial activities. The study aimed at isolation and characterisation of phytochemicals from the leaf extracts of ET.</p><h3>Results</h3><p>Three compounds were isolated from leaf extracts of ET and characterised. Two compounds; eriodictyol-7-<i>O</i>-β-glucoside (1) and 5, 7, 3′, 4′-tetrahydroxy-7-<i>O</i>-[6\"-<i>O</i>-(acetyl)-β-D-glucopyrancsyloxy]-flavanone (coccinoside A) (2) were identified from ethyl acetate extract while one compound; di-(2-ethylhexyl) phthalate (3) was identified from dichloromethane extract.</p><h3>Conclusion</h3><p>Compounds; 1, 2 and 3 were isolated and identified from ET and genus Erlangea for the first time. The reported antibacterial properties of compound 3 in literature could be responsible for the antibacterial activities of ET and the traditional use of ET.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00749-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectrophotometric and HPLC analysis of amoxicillin trihydrate in presence of acetaminophen in different pH media","authors":"Aditi Bala,&nbsp;Sanchita Mandal","doi":"10.1186/s43094-024-00747-6","DOIUrl":"10.1186/s43094-024-00747-6","url":null,"abstract":"<div><h3>Background</h3><p>This study is aimed to develop a simple, effective and economic method for the UV spectrophotometric analysis of amoxicillin trihydrate in the presence of acetaminophen. The Beer–Lambert law was obeyed in the concentration range of 2–10 µg/ml for amoxicillin trihydrate, acetaminophen and combinations, in all the different pH media: pH 1.2, 6.8, 7.4 and neutral (double-distilled water). For the simultaneous equation method, the absorbance maxima of amoxicillin trihydrate was found at 228 nm, and for acetaminophen, it was found at 243 nm, after scanning the solutions in respective buffers.</p><h3>Result</h3><p>The standard curve of amoxicillin trihydrate and acetaminophen was plotted, and the correlation coefficient (R²) value was found to be in the range of 0.991–0.994 and 0.993–0.999, respectively. These two drugs were combined in a ratio of 5:3 (amoxicillin trihydrate: acetaminophen), and its absorbance maxima was discovered at 232 nm (isoabsorptive point), where its correlation coefficient was calculated from the standard curve which is in range of 0.993–0.996. The above-mentioned method was found to comply all the validation parameters as per the ICH guidelines such as accuracy, precision, linearity, LOD, LOQ, reproducibility and recovery. This method is successfully applied to estimate the combination of these two drugs in their pharmaceutical dosage forms without and interaction of their excipients. This method is based on to check the stability of amoxicillin trihydrate in different pH media in the presence of acetaminophen.</p><h3>Conclusion</h3><p>Hydrolysis of beta-lactam ring of amoxicillin trihydrate occurs in acidic pH (below 2) which causes the formation of amoxicilloic acid which may cause reduction in its microbial activity but neither shifting of wavelength nor appearance of extra peak occurred in UV spectroscopy. Although some changes of % area of amoxicillin trihydrate is observed in acidic media in HPLC method, there are no significant changes observed among the amoxicillin trihydrate solutions with acetaminophen prepared in different pH media, when using UV spectrophotometric method.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00747-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, partial characterization, therapeutic, and safety evaluation of carbapenem-resistant Acinetobacter baumannii lytic phage in a mouse model","authors":"Hany S. Ahmed,&nbsp;Mohammed A. Eid,&nbsp;Amal M. Abo Kamer,&nbsp;Eman A. Hatem,&nbsp;Galal Yahya,&nbsp;Yehia A.-G. Mahmoud","doi":"10.1186/s43094-024-00744-9","DOIUrl":"10.1186/s43094-024-00744-9","url":null,"abstract":"<div><h3>Background</h3><p>Antimicrobial resistance (AMR) is a major worldwide health concern, characterized by the ability of microorganisms to withstand the effects of medications that once effectively treated infections. Phage therapy has emerged as a promising alternative for management of multidrug-resistant (MDR) bacterial infections. <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) exemplifies the emergence of bacteria resistant to clinically relevant antimicrobials, leading to severe nosocomial infections and exhibiting extensive and pan drug-resistant (XDR and PDR) traits. In response, this study isolated <i>A. baumannii</i> virulent phage designated as vB_AbaP_PhE54 against carbapenem-resistant <i>A. baumannii</i> (CRAB) pathogen and examined its morphological characteristics using an electron micrograph. Phage stability at different temperatures, pH, chloroform, safety, therapeutic evaluation, and growth kinetics have been analyzed.</p><h3>Results</h3><p>The <i>A. baumannii</i> phage vB_AbaP_PhE54 belongs morphologically to the <i>Podoviridae</i> family with very short, noncontractile tails, the phage demonstrated high thermal tolerance and infectivity across a pH range of 4–11, although it displayed a narrow host range. One-step growth kinetics indicated a burst size of 85 PFU (Plaque Forming Unit) per infected cell and a latent period of 20 min. Additionally, therapeutic efficiency in a mouse model showed total elimination of CRAB pathogen from lungs homogenates of mice and recovery from lung inflammation in all infected mice. On the other hand, safety evaluation of isolated phage revealed no adverse effects on structural or morphological tissue integrity.</p><h3>Conclusions</h3><p>These findings suggest that <i>A. baumannii</i> phage vB_AbaP_PhE54 could be a viable safe therapeutic option against <i>A. baumannii</i> infections, warranting further research into its clinical applications.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00744-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}