Future Journal of Pharmaceutical Sciences最新文献

{"title":"Health implications of prediabetes and the role of trace elements in insulin resistance","authors":"Hanan Mohamed Amer,&nbsp;Sherihan AboElyazed Mohamed,&nbsp;Farida Elshafeey,&nbsp;Salah Hussein Elhalawany","doi":"10.1186/s43094-025-00811-9","DOIUrl":"10.1186/s43094-025-00811-9","url":null,"abstract":"<div><h3>Background</h3><p>Prediabetes is more than just a direct cause of diabetes; it is a harmful condition linked to pathological alterations in various tissues and organs, highlighting its importance as a unique medical condition. It can regress if managed promptly to prevent progression to diabetes.</p><h3>Aims and objectives</h3><p>The aim of this review is to explore the complex relationship between variable trace elements and insulin resistance. Existing relationship may have a potential impact on the development and further progression of prediabetes. Analysis and synthesis of available research highlight how trace elements contribute to glucose derangement potentially hastening or mitigating the onset of diabetes.</p><h3>Methodology</h3><p>A systematic search of PUBMED, CENTRAL, SCOPUS, and Web of Science electronic databases was performed and all types of studies were included and no language restriction was applied. Identified titles and abstracts were screened to select original reports and any duplicates were removed.</p><h3>Conclusion</h3><p>The review summarizes pathophysiology, complications, and management of prediabetes, which makes clinicians aware and able to intervene at an early stage reducing the economic burden of overt diabetes. Additionally, it highlights the relationship between trace elements and their role in development of insulin resistance.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00811-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated bioinformatics and immunoinformatics approach to design a multi-epitope-based vaccine against Langya henipavirus","authors":"Saurav Kumar Mishra,&nbsp;Gyan Prakash Rai,&nbsp;Neeraj Kumar,&nbsp;Asheesh Shanker,&nbsp;John J. Georrge","doi":"10.1186/s43094-025-00815-5","DOIUrl":"10.1186/s43094-025-00815-5","url":null,"abstract":"<div><h3>Background</h3><p>In July 2022, a newly emerged viral infection called Langya virus, a type of <i>Henipavirus</i> identified in febrile patients in China and closely linked to two other <i>henipaviruses</i> (Hendra and Nipah) was considered a potential threat and can lead to the endemic situation. At present, no appropriate vaccine exists. Therefore, this investigation aims to design a multi-epitope vaccine against this infection via an integrated bioinformatics and immunoinformatics approach focusing on attachment glycoprotein and fusion protein.</p><h3>Results</h3><p>A total of 26 immunodominant epitopes were carefully chosen for vaccine formulation grounded on their antigenic, nonallergenic and nontoxic features and linked via precise linkers, along with HIV-TAT peptide, PADRE epitope and 6 × His-tag. The intended vaccine is forecast to be immunodominant, with broader population coverage encouraging physicochemical features and highly soluble. The 3D structure was anticipated and verified, and a docking study with toll-like receptors (TLR2, TLR3, TLR8 and TLR9) indicates significant binding with TLR3 and TLR9 based on the highest molecular interaction and high binding affinity score of − 25.2 and − 24.2 kcal mol⁻¹. NMA analysis revealed that vaccines with TLR3 and TLR9 have eigenvalues of 1.953251e−05 and 4.814201e−05, indicating proper molecular motion and flexibility. Further, the simulation (100 ns) showed constancy of complex (vaccine with TLR3 and TLR9). The generated immune activity indicates that the vaccines can trigger an intense immunological response. Furthermore, in silico cloning ensured a significant expression, followed by CAI values of 1 and GC (53.78%) content.</p><h3>Conclusion</h3><p>This study successfully designed a promising vaccine with strong immune activity. The vaccine revealed strong activity towards TLR3 and TLR9, with binding affinity of − 25.2 and − 24.2 kcal mol⁻¹, and over 100-ns simulation demonstrated minor deviation followed by the range of RMSD value. Further, the immune stimulation and cloning demonstrated potent activity and suggested the vaccine is able to evoke immune activity. However, experimental and clinical analyses are essential to authenticate these findings.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00815-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Plackett–Burman and Box–Behnken designs to develop a sensitive and robust HPLC method for quantifying hypolipidemic drugs, rosuvastatin and bempedoic acid in tablets","authors":"Susmitha Aggarapu,&nbsp;Rajitha Galla,&nbsp;Neha Jabeen Shaik,&nbsp;Sai Jyothi Dasari","doi":"10.1186/s43094-025-00814-6","DOIUrl":"10.1186/s43094-025-00814-6","url":null,"abstract":"<div><h3>Background</h3><p>Analytical quality by design approach is a systematic and scientific method for developing robust analytical procedures. Applying QbD principles in developing stability-indicating HPLC method for quantifying rosuvastatin and bempedoic acid in tablets enhances method understanding by identifying critical method parameters and analytical attributes, ensuring consistent quality.</p><h3>Method</h3><p>A two-level seven-factor Plackett–Burman design was employed to screen method parameters. Based on Pareto ranking analysis, the % aqueous (%v/v), buffer pH, and flow rate (ml/min) were identified as critical method parameters, while the retention times of rosuvastatin and bempedoic acid and resolution were selected as critical analytical attributes. Optimization was conducted using a two-level three-factor Box–Behnken design. The final optimized method utilized a Spursil C18 column (5 µm, 150 × 4.6 mm) with a mobile phase consisting of 15 mM ammonium acetate buffer (pH 6.0) and acetonitrile (40:60%v/v), a flow rate of 1 mL/min, and UV detection at 244 nm with an 8-min run time.</p><h3>Results</h3><p>Under the above conditions, rosuvastatin and bempedoic acid had retention times of 2.474 min and 3.396 min, respectively. Validation of the optimized method followed ICH Q2 (R1) guidelines and included system suitability, specificity, linearity, accuracy, precision, detection limit, quantitation limit, and robustness assessments. Linearity was observed in the range of 0.8–4.0 µg/mL for rosuvastatin and 3.6–18 µg/mL for bempedoic acid. Additionally, the greenness of the method was evaluated using AGREE software, which provided a score of 0.72, indicating compliance with Green Analytical Chemistry principles.</p><h3>Conclusion</h3><p>The developed RP-HPLC method is both sensitive and robust for the quantification of rosuvastatin and bempedoic acid in tablet formulations. The use of Plackett–Burman and Box–Behnken designs facilitated efficient optimization, and the method meets ICH guidelines and greenness criteria, confirming its suitability for routine analysis in quality control laboratories.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00814-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of TLR4 and upregulation of HO-1 & PPAR-γ by Pioglitazone ameliorates methotrexate-induced liver damage in rats","authors":"Rasha Abdelhady,&nbsp;Rana M. Abdelnaby,&nbsp;Mohamed Elsayed Mohamed Amer,&nbsp;Nancy S. Younis,&nbsp;Ebtehal Mohammad Fikry","doi":"10.1186/s43094-025-00819-1","DOIUrl":"10.1186/s43094-025-00819-1","url":null,"abstract":"<div><h3>Background</h3><p>Methotrexate is a frequently prescribed antifolate immunosuppressant and antineoplastic agent that has been associated with serious systemic adverse effects including hepatotoxicity. The current investigation explored the efficacy of the peroxisome proliferator activated receptor-gamma (PPAR-γ) agonist, Pioglitazone, in modulating Methotrexate-provoked liver damage then elucidating the underlying molecular mechanisms. Rats were allocated into four groups (<i>n</i> = 6): control group (received saline orally); Pioglitazone-exposed group (administered Pioglitazone 4 mg/kg/day p.o. from day 15 to 28); Methotrexate-treated group, (received Methotrexate 14 mg/kg/week p.o. from day 1 to 14); and Methotrexate and Pioglitazone-treated group (received Methotrexate form day 1 to 14 then received Pioglitazone from day 15 to 28 at the previously specified doses). </p><h3>Results</h3><p>The findings of the current work demonstrated that Pioglitazone alleviated Methotrexate-induced liver injury as depicted by correcting Methotrexate-induced elevation of liver enzymes, namely, alanine aminotransferase plus aspartate aminotransferase as well as ameliorating Methotrexate-induced histopathological changes. Accordingly, Pioglitazone administration in Methotrexate-intoxicated rats partially restored the redox homeostasis as manifested by suppressing malondialdehyde alongside elevating reduced glutathione contents. Notably, all previously mentioned parameters were measured using colorimetric assays. Remarkably, the reported hepatoprotective effect is putatively mediated through hindering hepatic inflammation reflected by the reported upregulation of PPAR-γ and hemoxygenase-1 with subsequent suppression of nuclear factor-kappa B and tumor necrosis factor-α. Additionally, current findings revealed modulation of Toll-like receptor 4 following Pioglitazone treatment that was further confirmed by our in silico study. </p><h3>Conclusions</h3><p>Therefore, this investigation suggests Pioglitazone as a promising therapeutic intervention in mitigating Methotrexate-induced liver injury.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00819-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking, antimicrobial, antibiofilm formation, and antiproliferative activities of furano-based sesquiterpenoids from Euryops arabicus","authors":"Fayza A. Aljohany,&nbsp;Samyah D. Jastaniah,&nbsp;Fatima B. Alamri,&nbsp;Nahed O. Bawakid,&nbsp;Hanan I. Althagbi,&nbsp;Ashraf B. Abdel-Naim,&nbsp;Mohammad Y. Alfaifi,&nbsp;Magda M. Aly,&nbsp;Fitri Budiyanto,&nbsp;Walied M. Alarif","doi":"10.1186/s43094-025-00810-w","DOIUrl":"10.1186/s43094-025-00810-w","url":null,"abstract":"<div><h3>Background</h3><p><i>Euryops arabicus</i> is well known in the Arabian Peninsula as traditional herbal medicine. The plant synthesizes various types of flavonoids, terpenes, and furoermophilanes skeletons in either steroidal or nonsteroidal conformation, and this wide range of chemical compounds might play an increasingly prominent role in medicine. This study aimed to isolate pure secondary metabolites and assess their antibacterial, antiproliferation, and antibiofilm formation properties.</p><h3>Methods</h3><p>The organic extract of the areal parts of <i>E. arabicus</i> was chromatographically fractionated to afford pure compounds characterized using NMR, UV, and IR along with mass spectrometry. The activity of the isolated compounds was assessed against three cancer cells and a set of Gram-negative and positive bacteria.</p><h3>Results</h3><p>Four specific sesquiterpenoids were identified: 6β-senecioyloxy-1,10-dehydrofuranoeremophilan-9-one (<b>1</b>), 6β-acetoxy-9-oxoeuryopsin (<b>2</b>), 6β-(2′-methyl, 2′,3′-epoxy-butyryloxy)-euryopsin (<b>3</b>), and 6β-angloyloxy-1,10-dehydrofuraoeremophilan-9-one (<b>4</b>). Molecular docking studies indicated the epidermal growth factor receptor and methionyl-tRNA synthetase as probable antibacterial and antiproliferative activities of the compounds. In the in vitro studies, compounds <b>2</b> and<b> 4</b> showed remarkable activity against all examined Gram-negative and positive pathogens with inhibition diameters (mm) ranging from 13.1 ± 0.23 to 19.0 ± 0.44 and 12.9 ± 0.20 to 16.2 ± 0.23, respectively. Compounds <b>2</b> and <b>4</b> showed moderate biofilm formation inhibition of both <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> with 58% and 79%, respectively. Moreover, the isolated sesquiterpenoids showed potent antiproliferative activities against MCF-7, HepG2, and HCT116 human cancer cells.</p><h3>Conclusion</h3><p>Four sesquiterpenoids were isolated from <i>E. arabicus</i>. Compound <b>2</b> showed a relatively high antibacterial and cytotoxic activity. Additional studies are recommended to substantiate the observed activities further.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00810-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized quercetin-loaded glycerohyalurosome hydrogel: an innovative nanoplatform for enhanced wound healing","authors":"Walaa Abualsunun,&nbsp;Amerh Aiad Alahmadi,&nbsp;Bayan A. Eshmawi,&nbsp;Osama A. A. Ahmed,&nbsp;Alaa Sirwi,&nbsp;Mahmoud A. Elfaky,&nbsp;Sarah O. Alreeshi,&nbsp;Ibtisam A. Alzahrani,&nbsp;Ibtihal A. Almutairi,&nbsp;Rumaysaa M. Gurunfula,&nbsp;Omaima N. Elgazayerly,&nbsp;Shaimaa M. Badr-Eldin","doi":"10.1186/s43094-025-00808-4","DOIUrl":"10.1186/s43094-025-00808-4","url":null,"abstract":"<div><h3>Background</h3><p>Lipidic nanovesicular systems have attracted researchers’ interest for more effective cutaneous delivery and topical pharmacological efficacy. Quercetin (QUT), a polyphenolic flavonoid known for its antioxidant and anti-inflammatory activity, suffers from poor solubility and bioavailability. The aim of this research was to develop an optimized hydrogel formulation comprising QUT-loaded hyaluronic acid (HYA)-modified glycerosomes (glycerohyalurosomes, GHEs) for effective wound management. A combination of glycerol (GLY) and HYA is being used to provide flexibility to the vesicles for better delivery through the skin; these compounds have been reported to provide benefits for wound healing. </p><h3>Results</h3><p>D-optimal design suggested fifteen formulations of QUT-GHEs which were prepared using a modified thin-film hydration method. Results showed that particle sizes ranged from 162.33 to 478.49 nm and zeta potential from −57.8 to −18.8 mV. Transmission electron microscopy confirmed successful loading of the drug into the vesicles. QUT-GHEs were integrated into hydrogel (QUT-GHE-GEL) using 1.5% hydroxypropyl methylcellulose. The pH of the QUT-GHE-GEL was recorded as 5.9 ± 0.03, which is acceptable in wound healing. In vivo studies performed on Wistar rats showed that QUT-GHE-GEL accelerated the wound-healing process compared to the untreated control and marketed product (MP)-treated groups, where a significantly higher wound contraction was observed. Histopathological examination of wound tissues revealed that QUT-GHE-GEL-treated and MP-treated groups exhibited newly sprouted capillaries and enhanced fibroblast development. </p><h3>Conclusions</h3><p>Thus, the suggested QUT-GHE-GEL formulation shows promise for effective wound-healing management. QUT-GHE-GEL enhances wound contraction and fosters tissue regeneration while modulating inflammation. The results indicate that QUT-GHE-GEL proves a prospective therapeutic option for wound care applications.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00808-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Syzygium australe extracts exhibit significant antioxidant and antidiabetic properties: a comprehensive analysis of the phytoconstituents","authors":"Samah A. Youssef,&nbsp;Ahmed H. Elosaily,&nbsp;Nermeen F. Farag,&nbsp;Nabil Mohamed Selim,&nbsp;Mohammed A. Hussein,&nbsp;Hala M. El Hefnawy","doi":"10.1186/s43094-025-00806-6","DOIUrl":"10.1186/s43094-025-00806-6","url":null,"abstract":"<div><h3>Background</h3><p>Medicinal plants are well known for their health benefits. <i>Syzygium australe</i> (Brush Cherry) is considered a health-promoting food due to its high antioxidant capacity, which may help prevent cancer, cardiovascular diseases, and neurological disorders. Additionally, <i>S. australe</i> exhibits antidiabetic and antiobesity properties. </p><h3>Aim</h3><p>This study reviews the antioxidant and antidiabetic activities of <i>S. australe</i> fractions, along with their key phytoconstituents.</p><h3>Materials and methods </h3><p>The antioxidant activity of <i>S. australe</i> crude extract and fractions was assessed using DPPH (2,2-diphenyl-1-picrylhydrazyl-hydrate), ABTS (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)), FIC (ferrozine iron metal chelation), and FRAP (ferric reducing antioxidant power) assays. Their antidiabetic activity was evaluated using <i>α</i>-glucosidase, <i>α</i>-amylase, and lipase inhibition assays. Additionally, their TFC (total flavonoid content) and TPC (total phenolic content) were determined. The secondary metabolites were analysed using Triple TOF UPLC-ESI–MS/MS (ultra-performance liquid chromatography-electrospray ionization-time of flight-mass spectrometry).</p><h3>Results</h3><p>Our findings, for the first time, reveal that various <i>S. australe</i> extracts exhibit strong antidiabetic and antioxidant properties. The butanol and ethyl acetate fractions had the highest antidiabetic activity. Their respective IC₅₀ values against <i>α</i>-glucosidase, <i>α</i>-amylase, and lipase were 0.11–0.14 µg/mL, 0.06–0.09 µg/mL, and 26.5–30.79 ng/mL, respectively. Also, they showed powerful antioxidant activity. The IC₅₀ values of the ethyl acetate (19.93 ± 1 μg/mL) and butanol (38.7 μg/mL) fractions were significantly lower than those of the reference compounds, Trolox (24.42 ± 0.87 μg/mL) in the DPPH assay and EDTA (Ethylenediaminetetraacetic acid) (39.7 μg/mL) in the ferrozine iron metal chelation assay, respectively. They had a higher amount of TPC (397.65 ± 14.78, 253.32 ± 10.78 µg GAE/mg sample) and TFC (97.89 ± 4.98, 23.14 ± 1.11 µg RE/mg sample) of ethyl acetate and butanol, respectively. The significant phenolic and flavonoid substances found in these extracts may account for their strong antioxidant activity. Triple TOF UPLC-ESI–MS/MS analysis detected 18 phenolic acids, 19 derivatives, and 39 flavonoids. As a way to prevent or treat various disorders, these two extracts additionally served as a great source of antioxidant phytochemicals.</p><h3>Conclusion</h3><p>Ultimately, our findings strongly suggest that the phenolics of <i>S. australe</i> may be valuable for the development of antioxidant and antidiabetic medications.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00806-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the possible ameliorative effects of beta-caryophyllene oxide either alone or in combination with methotrexate in complete Freund's adjuvant-induced arthritis in Wistar rats","authors":"Ammara Saleem,&nbsp;Maira Javed,&nbsp;Muhammad Furqan Akhtar,&nbsp;Aisha Mobashar,&nbsp;Muhammad Imran Khan","doi":"10.1186/s43094-025-00807-5","DOIUrl":"10.1186/s43094-025-00807-5","url":null,"abstract":"<div><h3>Background</h3><p>β-Caryophyllene oxide (BCPO) has wooden odor so widely used as cosmetic and food additives. It is naturally found in various spice and food plant such as basil, black pepper, clove, salvia, and indian bay leaf. It is traditionally used to treat arthritis. The present study anticipated to appraise the anti-inflammatory and anti-arthritic potential of BCPO alone and in combination with methotrexate.</p><h3>Results</h3><p>Data from <i>in vitro</i> assays showed that BCPO exerted the highest percentage inhibition at 1600 μg/ml. Complete Freund’s Adjuvant-induced arthritis in Wistar rats treated with BCPO and its combination with methotrexate (MTX) revealed a notable restoration of body weight and reduced pain, arthritic score, oxidative stress, and hematological alterations in contrast to disease control. The histopathological examination showed that BCPO therapy decreased joint inflammation, pannus formation, and bone erosion. Biochemical studies revealed that BCPO alone and in combination not only downregulated the mRNA expression of TNF-α, IL-1β, NF-kB, and COX-2 but also increased the expression of IL-4, and IL-10.</p><h3>Conclusion</h3><p>It can be inferred from the finding that BCPO in combination with MTX can be effectively used to manage polyarthritis due to notable anti-inflammatory, antioxidant, and anti-nociceptive activities than monotherapy.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00807-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability-indicating UPLC method for quantification of alpelisib in bulk and tablet formulation by QbD approach","authors":"Tandrima Majumder,&nbsp;Shiva Kumar Gubbiyappa,&nbsp;Padmini Iriventi","doi":"10.1186/s43094-025-00802-w","DOIUrl":"10.1186/s43094-025-00802-w","url":null,"abstract":"<div><h3>Background</h3><p>In the present study, a new and sensitive UPLC method for stability-indicating study has been established and validated as per the ICH guidelines. To date, no work has been reported on the forced degradation studies of alpelisib using the UPLC technique. Box–Behnken design was used to study the response surface for method optimisation to achieve a good separation with a minimum number of experimental trials. Three independent parameters selected were mobile phase ratio, flow rate of the mobile phase and temperature of the column. Retention time and tailing factor were taken as two responses to obtain mathematical models.</p><h3>Results</h3><p>The chromatography was executed using Waters BEH C₁₈ UPLC column (2.1 × 50 mm, 1.7micron) at wavelength detection of 246 nm. The optimised assay conditions predicted were isocratic mobile solvent system using 0.1% formic acid buffer solution and acetonitrile in the ratio of 50:50 (V/V), with a flow rate of 0.25 mL per min and injection volume of 4 µL. The method has shown a good response with a total run time of 4 min, retention time was found to be 1.49 min and tailing factor was 0.99 for alpelisib, showing good peak symmetry. The linearity of the method developed is at a range of 10–50 µg/mL with R² 0.9955, while the percentage mean recovery for accuracy was of 99.25%. The method developed was precise as % RSD of inter-day and intra-day precision was 0.3 and 0.1, respectively, meeting the specified limits.</p><h3>Conclusion</h3><p>The developed UPLC work is quick and simple with an increased level of linearity, precision, specificity and accuracy as per the ICH criteria and can find application in industries as a regular method for quantification of alpelisib.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00802-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current perspectives on malnutrition and immunomodulators bridging nutritional deficiencies and immune health","authors":"Ashish Majumdar,&nbsp;Surendra Kumar Saraf,&nbsp;Chandrashekhar Sahu,&nbsp;Khushboo Verma,&nbsp;Priyanka Vishwakarma","doi":"10.1186/s43094-025-00804-8","DOIUrl":"10.1186/s43094-025-00804-8","url":null,"abstract":"<div><h3>Background</h3><p>Malnutrition is still one of the most serious and prevalent worldwide health problems, especially found in low- and middle-income countries, which impairs immune functions and increases susceptibility to infection. This study investigates the complex association between malnutrition and immune dysfunction, and the role of immunomodulators in restoring immune function. This study analyzes the different types of malnutrition, including protein-energy malnutrition and micronutrient deficiencies, and their consequences to the immune system through inhibited cytokine and immune cell production.</p><h3>Main body</h3><p>Immunomodulators, which include natural agents such as phytochemicals and probiotics, as well as synthetic agents, may help reduce immune dysfunction related to starvation. This article categorizes these agents and discusses their mechanisms of action, including their role in regulating inflammatory pathways, increasing the generation of immune cells, and augmenting global immune response. In addition, therapeutic approaches utilizing immunomodulation in conjunction with nutritional therapies, such as micronutrient supplementation (vitamins A, C, D and zinc) or natural immunomodulators, to improve inflammatory and gastroenterological disease states are discussed. Case reports and recent studies are provided that provide evidence supporting the effectiveness of immunomodulation and nutritional therapy to improve clinical outcomes in vulnerable populations.</p><h3>Conclusion</h3><p>While there is a promise for immunomodulators, there are safety, long-term efficacy, and ethical issues to address before they could widely be employed. Each step of the research calls for applied, working example of immunomodulatory medicine that could be tailored to health programs internationally. The research highlights the vital role of immunomodulators in the treatment of malnutrition and encourages holistic approaches to improve immunological health worldwide.</p></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"11 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-025-00804-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}