系统微生物学与生物制造(英文)最新文献_第7页

In silico comparative structural and compositional analysis of glycoproteins of RSV to study the nature of stability and transmissibility of RSV A. RSV糖蛋白的计算机比较结构和组成分析，以研究RSV A的稳定性和传播性

系统微生物学与生物制造(英文) Pub Date : 2023-01-01 Epub Date: 2022-05-27 DOI: 10.1007/s43393-022-00110-x

Debanjan Mitra, Pradeep K Das Mohapatra

{"title":"In silico comparative structural and compositional analysis of glycoproteins of RSV to study the nature of stability and transmissibility of RSV A.","authors":"Debanjan Mitra, Pradeep K Das Mohapatra","doi":"10.1007/s43393-022-00110-x","DOIUrl":"10.1007/s43393-022-00110-x","url":null,"abstract":"The current scenario of COVID-19 makes us to think about the devastating diseases that kill so many people every year. Analysis of viral proteins contributes many things that are utterly useful in the evolution of therapeutic drugs and vaccines. In this study, sequence and structure of fusion glycoproteins and major surface glycoproteins of respiratory syncytial virus (RSV) were analysed to reveal the stability and transmission rate. RSV A has the highest abundance of aromatic residues. The Kyte-Doolittle scale indicates the hydrophilic nature of RSV A protein which leads to the higher transmission rate of this virus. Intra-protein interactions such as carbonyl interactions, cation-pi, and salt bridges were shown to be greater in RSV A compared to RSV B, which might lead to improved stability. This study discovered the presence of a network aromatic-sulphur interaction in viral proteins. Analysis of ligand binding pocket of RSV proteins indicated that drugs are performing better on RSV B than RSV A. It was also shown that increasing the number of tunnels in RSV A proteins boosts catalytic activity. This study will be helpful in drug discovery and vaccine development.","PeriodicalId":56711,"journal":{"name":"系统微生物学与生物制造(英文)","volume":"3 1","pages":"312-327"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9135598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49601007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic bio-fabrication of aptamers and their applications in engineering biology. 适体的系统制备及其在工程生物学中的应用

系统微生物学与生物制造(英文) Pub Date : 2023-01-01 Epub Date: 2022-10-10 DOI: 10.1007/s43393-022-00140-5

Rongfeng Cai, Xin Chen, Yuting Zhang, Xiaoli Wang, Nandi Zhou

{"title":"Systematic bio-fabrication of aptamers and their applications in engineering biology.","authors":"Rongfeng Cai, Xin Chen, Yuting Zhang, Xiaoli Wang, Nandi Zhou","doi":"10.1007/s43393-022-00140-5","DOIUrl":"10.1007/s43393-022-00140-5","url":null,"abstract":"Aptamers are single-stranded DNA or RNA molecules that have high affinity and selectivity to bind to specific targets. Compared to antibodies, aptamers are easy to in vitro synthesize with low cost, and exhibit excellent thermal stability and programmability. With these features, aptamers have been widely used in biology and medicine-related fields. In the meantime, a variety of systematic evolution of ligands by exponential enrichment (SELEX) technologies have been developed to screen aptamers for various targets. According to the characteristics of targets, customizing appropriate SELEX technology and post-SELEX optimization helps to obtain ideal aptamers with high affinity and specificity. In this review, we first summarize the latest research on the systematic bio-fabrication of aptamers, including various SELEX technologies, post-SELEX optimization, and aptamer modification technology. These procedures not only help to gain the aptamer sequences but also provide insights into the relationship between structure and function of the aptamers. The latter provides a new perspective for the systems bio-fabrication of aptamers. Furthermore, on this basis, we review the applications of aptamers, particularly in the fields of engineering biology, including industrial biotechnology, medical and health engineering, and environmental and food safety monitoring. And the encountered challenges and prospects are discussed, providing an outlook for the future development of aptamers.","PeriodicalId":56711,"journal":{"name":"系统微生物学与生物制造(英文)","volume":"3 1","pages":"223-245"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9550155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48991168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Human lactate dehydrogenase and malate dehydrogenase possess the catalytic properties to produce aromatic α-hydroxy acid 人乳酸脱氢酶和苹果酸脱氢酶具有催化生成芳族α-羟基酸的特性

系统微生物学与生物制造(英文) Pub Date : 2022-12-22 DOI: 10.1007/s43393-022-00154-z

Litong Wang, Tai-Ping Fan, Mei Wang, Yajun Bai, Xiaohui Zheng, Yujie Cai