中国疫苗和免疫最新文献_第4页

[Research progress on DNA tumor vaccine]. [DNA肿瘤疫苗研究进展]。

中国疫苗和免疫 Pub Date : 2010-04-01

Zhen-wei Xue, Xiao-lin Zhou

引用次数: 0

[Hepatitis B and hepatocellular carcinoma]. [乙肝和肝细胞癌]。

中国疫苗和免疫 Pub Date : 2010-04-01

Ai-wu Sun, Yuan-sheng Chne, Li Li

引用次数: 0

[Analysis of 4 clustered high risk acute flaccid paralysis cases in Shanxi Province in 2006]. [2006年山西省聚集性高危急性弛缓性麻痹4例分析]。

中国疫苗和免疫 Pub Date : 2010-04-01

Dong-mei Yan, Yong Zhang, Dong-yan Wang

{"title":"[Analysis of 4 clustered high risk acute flaccid paralysis cases in Shanxi Province in 2006].","authors":"Dong-mei Yan, Yong Zhang, Dong-yan Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Analysis of epidemiology of 4 clustered high risk acute flaccid paralysis(AFP) cases reported by Shanxi province in 2006 and VP1 gene characteristic for type III poliovirus isolated from the four AFP cases.Methods: Virus isolation and identification were conducted according to the 4th edition of WHO polio laboratory manual. The sequence of VP1 region were amplified and sequenced. The phylogenetic trees based on VP1 region were constructed.Result: Three of four high risk AFP cases were suspected as vaccine associated paralysis poliomyelitis (VAPP), the onset date of them were close. VP1 sequencing of the four type III isolates revealed that the identity were 99.7%, 99.9%, 99.4% and 99.9% respectively compared with vaccine reference strain-BJOPV3. According to WHO criteria, the four isolates were identified as type III vaccine-related poliovirus. Phylogenetic analysis based on VP1 coding sequence showed that the four type III poliovirus were not related significantly. The type III poliovirus isolated from 3 suspected VAPP cases shared one nucleotide mutation at 2637 (C-->U), which result in the amino acid mutation from Val into Ala.Conclusion: The improvement of laboratory surveillance for clustered high risk AFP cases should be strengthened so as to detect and prevent poliovirus circulation timely.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Measles antibody level of productive women in Hongkou District of Shanghai Municipal]. 上海市虹口区育龄妇女麻疹抗体水平分析

中国疫苗和免疫 Pub Date : 2010-04-01

Xiao-hua Qian, Ping Yang, Su-zhen Tang

引用次数: 0

[A prospective cohort study on community children vaccinated with Haemophilus influenzae type b]. [一项针对社区儿童接种b型流感嗜血杆菌的前瞻性队列研究]。

中国疫苗和免疫 Pub Date : 2010-04-01

Xiao-hua Qian, Chuan-qing Wang, Qi-rong Zhu

引用次数: 0

[Running and surveillance status of measles laboratory network in Gansu province in 2008]. [2008年甘肃省麻疹实验室网络运行与监测现状]。

中国疫苗和免疫 Pub Date : 2010-04-01

Hong Fu, Xiao-rong Zhen, Jian-feng Liu

引用次数: 0

[Phylogenetic analysis based on hemagglutinin and neuraminidase genes between swine influenza virus and human influenza A/H3N2 virus strains isolated in Zhejiang province]. [基于浙江省猪流感病毒与人流感A/H3N2病毒血凝素和神经氨酸酶基因的系统发育分析]。

中国疫苗和免疫 Pub Date : 2010-04-01

Li-pei Qiu, Yi-yu Lu, Chang-ping Xu

{"title":"[Phylogenetic analysis based on hemagglutinin and neuraminidase genes between swine influenza virus and human influenza A/H3N2 virus strains isolated in Zhejiang province].","authors":"Li-pei Qiu, Yi-yu Lu, Chang-ping Xu","doi":"","DOIUrl":"","url":null,"abstract":"Objectives: To investigate the phylogenetic relationship between swine influenza A/H3N2 virus and the representative strains of human influenza A/H3N2 virus isolated in two epidemics in recent years through comparing the sequences within HA and NA genes.Methods: HA and NA gene of the human representative strains were sequenced, and then phylogenetic tree with the swine and human strains isolated in the corresponding period of time were constructed.Results: The homologies on the HA1 domain between human representative strains (A/Zhejiang/10/98, A/Zhejiang/6/99 and A/Zhejiang/8/02)and the swine strains (A/SW/Ontario/130/97, A/SW/Hongkong/4361/99 and A/SW/Hongkong/74/02) were 99.1%, 99.4% and 99.4% respectively. Based on the NA gene, the homologies between human strains (A/Zhejiang/10/98, _A/Zhejiang/6/99 and A/Zhejiang/8/02)and the swine strains (A/SW/Ontario/130/97, A/SW/Hongkong/4361/99 and A/SW/Hongkong/74/02) were 98.2%, 99.3% and 99.3% respectively. The results showed that the two types of influenza viruses were highly homologue, and even some of their homologies were higher than that amongst the contemporary human influenza A/H3N2 strains. The same results shown in the phylogenetic tree.Conclusions: The human influenza A/H3N2 virus isolates in the two epidemic closely associated with some of the swine influenza virus strains, and their relationship should be further studied.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29076593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Analysis on genetic characteristic of type I poliovirus in China in 2009]. 2009年中国ⅰ型脊髓灰质炎病毒遗传特征分析

中国疫苗和免疫 Pub Date : 2010-04-01

Jun-jing An, Hui Zhu, Dong-mei Yan

{"title":"[Analysis on genetic characteristic of type I poliovirus in China in 2009].","authors":"Jun-jing An, Hui Zhu, Dong-mei Yan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To study the molecular characteristics of type 1 poliovirus isolated from the acute flaccid paralysis (AFP)surveillance system in China in 2009, to provide a scientific basis for maintaining polio-free status for China.Method: Polymerase chain reaction (RT-PCR) method was used to amplify the VP1 code region of all the type I poliovirus, and the VP1 coding region of the isolated stains was sequenced and analyzed, the hot-spots and nuerovirulence determinant were analyzed. The phylogenetic tree was constructed based on VP1 region to analyze the evolutionary relationship between the strains.Result: The results of VP1 sequencing showed that no wild strains or vaccine-derived poliovirus (VDPVs) were detected. However, five pre-VDPVs were found. And nucleotide sequences of two isolates were in high degree of similarity (100%). Sequence alignment showed that two nucleotides in the VP1 region. nt2747 and nt2749 were two mutation hot spots.Conclusion: According to the epidemiological and laboratory test results of two high variation strains, the short-term circulation may occur probably, and further research are needed. Meanwhile, the existence of mutation hot spots indicated that strains are easy to reverse into wild-type substitutions, and lead to a series changes of neurological and other virulence when the strains are under selective pressure.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Evaluation of real time fluorescent quantitative reverse transcription polymerase chain reaction for detection of poliovirus]. 实时荧光定量逆转录聚合酶链反应检测脊髓灰质炎病毒的评价

中国疫苗和免疫 Pub Date : 2010-04-01

Dong-mei Yan, Hui Zhu, Jun-jing An

引用次数: 0

[Serum antibody response to different doses of hepatitis B vaccine made by recombinant DNA techniques in yeast and Hansenula polymorpha yeast]. [重组DNA技术在酵母和多形汉斯拉酵母中制备不同剂量乙型肝炎疫苗的血清抗体反应]。

中国疫苗和免疫 Pub Date : 2010-04-01

Jing Sun, Yue Han, Yan Wang

{"title":"[Serum antibody response to different doses of hepatitis B vaccine made by recombinant DNA techniques in yeast and Hansenula polymorpha yeast].","authors":"Jing Sun, Yue Han, Yan Wang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To observe the serum antibody response of the population, whose hepatitis B virus surface antigen(HBsAg), antibody to hepatitis B virus surface antigen (Anti-HBs), antibody to hepatitis B virus core antigen (Anti-HBc) are all negative to the different doses of Hepatitis B vaccine made by recombinant DNA techniques in Yeast and Hansenula Polymorpha Yeast.Methods: Collecting the blood of population vaccinated after 28 to 45 days, the serological method was used to detect Anti-HBs.Results: The Anti-HBs positive rate and GMC of 5 microg HepB-Y in the population <16 years old whose HBsAg, Anti-HBs, Anti-HBc negative are 97.23% and 199.26 mlU/ml, the Anti-HBs positive rate and GMC of 10 microg HepB-HPY are 100.00% and 270.71 mlU/ml, the Anti-HBs positive rate and GMC both have significant difference. The Anti-HBs positive rate and GMC of 10 microg HepB-Y in the population > or =16 years old whose HBsAg, Anti-HBs, Anti-HBc negative are 88.72% and 101.19 mlU/ml. Anti-HBs positive rate and GMC of 10 microg HepB-HPY are 94.20% and 162.17 mlU/ml, the Anti-HBs positive rate and GMC both have significant difference.Conclusions: The population whose HBsAg, Anti-HBs, Anti-HBc negative vaccinated by HepB-Y, HepB-HPY can get better Anti-HBs response. The population younger than sixteen years old vaccinated by 10 microg HepB-HPY can get Anti-HBs positive rate and GMC higher than vaccinated by 5 microg HepB-Y. The Anti-HBs response of HepB-HPY is better than HepB-Y in the population > or =16 years old.","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29078372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0