[Serum antibody response to different doses of hepatitis B vaccine made by recombinant DNA techniques in yeast and Hansenula polymorpha yeast].

Objective: To observe the serum antibody response of the population, whose hepatitis B virus surface antigen(HBsAg), antibody to hepatitis B virus surface antigen (Anti-HBs), antibody to hepatitis B virus core antigen (Anti-HBc) are all negative to the different doses of Hepatitis B vaccine made by recombinant DNA techniques in Yeast and Hansenula Polymorpha Yeast.

Methods: Collecting the blood of population vaccinated after 28 to 45 days, the serological method was used to detect Anti-HBs.

Results: The Anti-HBs positive rate and GMC of 5 microg HepB-Y in the population <16 years old whose HBsAg, Anti-HBs, Anti-HBc negative are 97.23% and 199.26 mlU/ml, the Anti-HBs positive rate and GMC of 10 microg HepB-HPY are 100.00% and 270.71 mlU/ml, the Anti-HBs positive rate and GMC both have significant difference. The Anti-HBs positive rate and GMC of 10 microg HepB-Y in the population > or =16 years old whose HBsAg, Anti-HBs, Anti-HBc negative are 88.72% and 101.19 mlU/ml. Anti-HBs positive rate and GMC of 10 microg HepB-HPY are 94.20% and 162.17 mlU/ml, the Anti-HBs positive rate and GMC both have significant difference.

Conclusions: The population whose HBsAg, Anti-HBs, Anti-HBc negative vaccinated by HepB-Y, HepB-HPY can get better Anti-HBs response. The population younger than sixteen years old vaccinated by 10 microg HepB-HPY can get Anti-HBs positive rate and GMC higher than vaccinated by 5 microg HepB-Y. The Anti-HBs response of HepB-HPY is better than HepB-Y in the population > or =16 years old.