Cancer Biomarkers最新文献_第8页

Rho GTPase-activating protein 4 is upregulated in Kidney Renal Clear Cell Carcinoma and associated with poor prognosis and immune infiltration. Rho GTPase-activating protein 4在肾透明细胞癌中上调，与预后不良和免疫浸润有关。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230388

Xuesong Xiao, Xiaofei Lv, Tianyu Lin, Jianqiao Li, Rui Wang, Shaoping Tian, Xinyu Liu, Shiming Liu, Huamao Jiang, Dan Yue, Yong Wang

{"title":"Rho GTPase-activating protein 4 is upregulated in Kidney Renal Clear Cell Carcinoma and associated with poor prognosis and immune infiltration.","authors":"Xuesong Xiao, Xiaofei Lv, Tianyu Lin, Jianqiao Li, Rui Wang, Shaoping Tian, Xinyu Liu, Shiming Liu, Huamao Jiang, Dan Yue, Yong Wang","doi":"10.3233/CBM-230388","DOIUrl":"10.3233/CBM-230388","url":null,"abstract":"Background: Kidney Renal Clear Cell Carcinoma (KIRC) is a malignant tumor that seriously threatens human health. Rho GTPase-activating protein 4 (ARHGAP4) plays an important role in the occurrence and development of tumors.Objective: The purpose of this study was to explore the role of ARHGAP4 in the progression of KIRC and its diagnostic and prognostic value.Methods: Multiple analytical methods and in vitro cell assays were used to explore the expression of ARHGAP4 and its value in the progression, diagnosis and prognosis of KIRC. The biological function of ARHGAP4 was studied by GO analysis and KEGG pathway analysis, and then the relationship between ARHGAP4 and immune infiltration was analyzed.Results: The expression of ARHGAP4 was significantly up-regulated in KIRC. We found that the high expression of ARHGAP4 was related to the progression of KIRC and suggested a poor prognosis. Compared with normal tissues, ARHGAP4 had a better diagnostic value in KIRC. The biological function of ARHGAP4 was related to immunity, and its expression was also closely related to tumor immune infiltration and immune checkpoints.Conclusions: Our study demonstrated that ARHGAP4 may be a biomarker, which is related to the progression, diagnosis and prognosis of KIRC. Its biological functions are related to tumor immune infiltration.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"205-223"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11307029/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypermethylation of the BRCA2 gene promoter and its co-hypermethylation with the BRCA1 gene promoter in patients with breast cancer. 乳腺癌患者 BRCA2 基因启动子的超甲基化及其与 BRCA1 基因启动子的共超甲基化。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230458

Liliia Fishchuk, Zoia Rossokha, Olga Lobanova, Valeriy Cheshuk, Roman Vereshchako, Viktoriia Vershyhora, Nataliia Medvedieva, Olha Dubitskaa, Natalia Gorovenko

{"title":"Hypermethylation of the BRCA2 gene promoter and its co-hypermethylation with the BRCA1 gene promoter in patients with breast cancer.","authors":"Liliia Fishchuk, Zoia Rossokha, Olga Lobanova, Valeriy Cheshuk, Roman Vereshchako, Viktoriia Vershyhora, Nataliia Medvedieva, Olha Dubitskaa, Natalia Gorovenko","doi":"10.3233/CBM-230458","DOIUrl":"10.3233/CBM-230458","url":null,"abstract":"Background: The BRCA2 gene is an important tumour suppressor in breast cancer, and alterations in BRCA2 may lead to cancer progression. The aim of the study was to investigate the association of hypermethylation of the BRCA2 gene promoter and its co-hypermethylation with the BRCA1 gene promoter with the development and course of breast cancer in women.Methods: This study included 74 women with breast cancer (tumour tissue samples and peripheral blood) and 62 women without oncological pathology (peripheral blood) - control group.Results: Hypermethylation of the BRCA2 gene was significantly more frequently detected in the tumour tissue of women with breast cancer compared to their peripheral blood and peripheral blood of control subjects (p= 0.0006 and p= 0.00001, respectively). Hypermethylation of BRCA2 was more frequently detected in patients with breast cancer over the age of 50 and in patients with higher Ki67 expression levels (p= 0.045 and p= 0.045, respectively). There was a high frequency of unmethylated BRCA1 and BRCA2 gene combination in women of the control group compared to women with breast cancer, both in blood samples and tumour tissue samples (p= 0.014 and p= 0.00001, respectively).Conclusion: Our study confirms the hypothesis that BRCA2 hypermethylation plays an important role in the pathogenesis of breast cancer and the importance of assessing its co-hypermethylation with BRCA1 in predicting the course of the disease.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"275-283"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142037853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The paradoxical role of transforming growth factor-β in controlling oral squamous cell carcinoma development. 转化生长因子-β在控制口腔鳞状细胞癌发展中的矛盾作用。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230354

Ruiting Peng, Yun Huang, Ping Huang, Linyi Liu, Lei Cheng, Xian Peng

引用次数: 0

Circ_0049271 targets the miR-1197/PTRF axis to attenuate the malignancy of osteosarcoma. Circ_0049271靶向miR-1197/PTRF轴，减轻骨肉瘤的恶性程度。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230191

Yixin Wen, Feng Xu, Hui Zhang

{"title":"Circ_0049271 targets the miR-1197/PTRF axis to attenuate the malignancy of osteosarcoma.","authors":"Yixin Wen, Feng Xu, Hui Zhang","doi":"10.3233/CBM-230191","DOIUrl":"10.3233/CBM-230191","url":null,"abstract":"Background: Circular RNAs (circRNAs) perform key regulatory functions in osteosarcoma (OS) tumorigenesis. In this study, we aimed to explore the detailed action mechanisms of circ_0049271 in OS progression.Methods: Cell colony formation, cell counting kit-8, and transwell assays were performed to assess the proliferation and invasion of OS cells. Quantitative reverse transcription-polymerase chain reaction and western blotting were used to determine the expression levels of polymerase 1 and transcript release factor (PTRF), microRNA (miR)-1197, and circ_0049271 in OS cells. Furthermore, RNA immunoprecipitation and dual luciferase assays were conducted to explore the targeted relationships among PTRF, miR-1197, and circ_0049271. Finally, a tumor formation assay was conducted to determine the effects of circ_0049271 on in vivo tumor growth in mice.Results: High expression levels of miR-1197 and low levels of circ_0049271 and PTRF were observed in OS cells. circ _0049271 targeted miR-1197 to mediate PTRF expression. Moreover, the proliferation and invasion of OS cells were repressed by circ_0049271 or PTRF overexpression and increased by miR-1197 upregulation. Enforced circ_0049271 also impeded tumor growth in vivo. Upregulation of miR-1197 reversed the antitumor effects of circ_0049271 on OS progression in vitro; however, PTRF overexpression attenuated the cancer-promoting effects of miR-1197 on OS in vitro.Conclusions: Our findings revealed that circ_0049271 targeted the miR-1197/PTRF axis to attenuate the malignancy of OS, suggesting a potential target for its clinical treatment.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"141-153"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11321495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140869425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GINS2 promotes the progression of human HNSCC by altering RRM2 expression. GINS2 通过改变 RRM2 的表达促进人类 HNSCC 的发展。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230337

Tianxiang Wang, Luxi Qian, Pingchuan Zhang, Mingyu Du, Jing Wu, Fanyu Peng, Chengyun Yao, Rong Yin, Li Yin, Xia He

{"title":"GINS2 promotes the progression of human HNSCC by altering RRM2 expression.","authors":"Tianxiang Wang, Luxi Qian, Pingchuan Zhang, Mingyu Du, Jing Wu, Fanyu Peng, Chengyun Yao, Rong Yin, Li Yin, Xia He","doi":"10.3233/CBM-230337","DOIUrl":"10.3233/CBM-230337","url":null,"abstract":"Introduction: GINS2 exerts a carcinogenic effect in multiple human malignancies, while it is still unclear that the potential roles and underlying mechanisms of GINS2 in HNSCC.Methods: TCGA database was used to screen out genes with significant differences in expression in HNSCC. Immunohistochemistry and qRT-PCR were used to measure the expression of GINS2 in HNSCC tissues and cells. GINS2 was detected by qRT-PCR or western blot after knockdown or overexpression. Celigo cell counting, MTT, colony formation, and flow cytometric assay were used to check the ability of proliferation and apoptosis. Bioinformatics and microarray were used to screen out the downstream genes of GINS2.Results: GINS2 in HNSCC tissues and cells was up-regulated, which was correlated with poor prognosis. GINS2 gene expression was successfully inhibited and overexpressed in HNSCC cells. Knockdown of GINS2 could inhibit proliferation and increase apoptosis of cells. Meanwhile, overexpression of GINS2 could enhance cell proliferation and colony formation. Knockdown of RRM2 may inhibit HNSCC cell proliferation, while overexpression of RRM2 rescued the effect of reducing GINS2 expression.Conclusion: Our study reported the role of GINS2 in HNSCC for the first time. The results demonstrated that in HNSCC cells, GINS2 promoted proliferation and inhibited apoptosis via altering RRM2 expression. Therefore, GINS2 might play a carcinogen in HNSCC, and become a specific promising therapeutic target.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"171-184"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11307040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140190468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum aspartate aminotransferase, a novel potential biomarker of prognosis in extranodal natural killer/T cell lymphoma, nasal type. 血清天冬氨酸氨基转移酶--鼻型结节外自然杀伤/T细胞淋巴瘤预后的潜在新型生物标志物

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230068

Ningning Yao, Qing Hou, Yu Liang, Xin Cao, Bochen Sun, Lijuan Wei, Ruifang Sun, Jianzhong Cao

{"title":"Serum aspartate aminotransferase, a novel potential biomarker of prognosis in extranodal natural killer/T cell lymphoma, nasal type.","authors":"Ningning Yao, Qing Hou, Yu Liang, Xin Cao, Bochen Sun, Lijuan Wei, Ruifang Sun, Jianzhong Cao","doi":"10.3233/CBM-230068","DOIUrl":"10.3233/CBM-230068","url":null,"abstract":"Background: Aspartate aminotransferase (AST), an indicator of liver cell damage, was related to the prognosis of certain malignant tumors.Objective: This study examined the predictive value of AST in patients with extranodal natural killer/T cell lymphoma (ENKTL).Methods: We reviewed 183 cases diagnosed with ENKTL and selected 26 U/L as the optimum cut-off value of AST. We used the univariate and multivariate Cox regression to compare the different AST groups' overall survival (OS) and progression-free survival (PFS).Results: Prior to propensity score matching (PSM), Kaplan-Meier analysis showed that patients in the low AST subgroup had better OS and PFS than the high AST subgroup. Multivariate analysis revealed that AST was an independent indicator for prognosis. After PSM, the low AST subgroup maintained a significantly better OS and PFS than the high AST subgroup.Conclusion: AST might represent a significant prognostic marker for ENKTL patients.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"265-275"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11191476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138813954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KIF18A promotes cervical squamous cell carcinoma progression by activating the PI3K/AKT pathway through upregulation of CENPE. KIF18A 通过上调 CENPE 激活 PI3K/AKT 通路，从而促进宫颈鳞状细胞癌的进展。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-240074

Fengyi Sun, Tiantian Zhao

{"title":"KIF18A promotes cervical squamous cell carcinoma progression by activating the PI3K/AKT pathway through upregulation of CENPE.","authors":"Fengyi Sun, Tiantian Zhao","doi":"10.3233/CBM-240074","DOIUrl":"10.3233/CBM-240074","url":null,"abstract":"Background: Cervical cancer is a prevalent malignancy that significantly contributes to morbidity and mortality rates among women in developing nations. Although the association of KIF18A with various cancers has been established, its role in cervical squamous cell carcinoma (CESC) remains elusive.Methods: The KIF18A impact on the progression of CESC and its underlying mechanism were investigated through comprehensive bioinformatics analysis utilizing publicly available datasets. The levels of KIF18A and CENPE were assessed in clinical CESC samples through western blotting and qRT-PCR. To discover the role and molecular pathways of KIF18A in CESC, a combination of experimental approaches, including wound-healing, flow cytometry, CCK-8, and Transwell assay, were employed.Results: Our results demonstrate a significant KIF18A expression upregulation in CESC tissues in contrast to healthy tissues. In vitro, KIF18A upregulation was found to enhance cell growth, migration, and invasion and activate the PI3K/AKT signaling pathway while concurrently suppressing apoptosis. Conversely, downregulating KIF18A exhibited contrasting effects. Mechanistically, we observed a positive significant connection between KIF18A and CENPE in CESC cells.Conclusion: KIF18A promotes tumor growth in CESC by modulating the PI3K/AKT signaling pathway through regulation of CENPE, making it a potential biomarker for diagnosis and prognosis as well as a therapeutic target.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"165-178"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glioma stem cells remodel immunotolerant microenvironment in GBM and are associated with therapeutic advancements. 胶质瘤干细胞重塑了胶质瘤的免疫耐受微环境，并与治疗进展有关。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230486

Xifeng Fei, Jie Wu, Haiyan Tian, Dongyi Jiang, Hanchun Chen, Ke Yan, Yuan Wang, Yaodong Zhao, Hua Chen, Xiangtong Xie, Zhimin Wang, Wenyu Zhu, Qiang Huang

{"title":"Glioma stem cells remodel immunotolerant microenvironment in GBM and are associated with therapeutic advancements.","authors":"Xifeng Fei, Jie Wu, Haiyan Tian, Dongyi Jiang, Hanchun Chen, Ke Yan, Yuan Wang, Yaodong Zhao, Hua Chen, Xiangtong Xie, Zhimin Wang, Wenyu Zhu, Qiang Huang","doi":"10.3233/CBM-230486","DOIUrl":"10.3233/CBM-230486","url":null,"abstract":"Glioma is the most common primary tumor of the central nervous system (CNS). Glioblastoma (GBM) is incurable with current treatment strategies. Additionally, the treatment of recurrent GBM (rGBM) is often referred to as terminal treatment, necessitating hospice-level care and management. The presence of the blood-brain barrier (BBB) gives GBM a more challenging or \"cold\" tumor microenvironment (TME) than that of other cancers and gloma stem cells (GSCs) play an important role in the TME remodeling, occurrence, development and recurrence of giloma. In this review, our primary focus will be on discussing the following topics: niche-associated GSCs and macrophages, new theories regarding GSC and TME involving pyroptosis and ferroptosis in GBM, metabolic adaptations of GSCs, the influence of the cold environment in GBM on immunotherapy, potential strategies to transform the cold GBM TME into a hot one, and the advancement of GBM immunotherapy and GBM models.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"1-24"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11492047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142141868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KLF5 inhibits the migration and invasion in cervical cancer cell lines by regulating SNAI1. KLF5 通过调节 SNAI1 抑制宫颈癌细胞株的迁移和侵袭。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230175

Xinjian Qu, Chang Xu, Wenbo Yang, Qianqian Li, Simei Tu, Chenghai Gao

{"title":"KLF5 inhibits the migration and invasion in cervical cancer cell lines by regulating SNAI1.","authors":"Xinjian Qu, Chang Xu, Wenbo Yang, Qianqian Li, Simei Tu, Chenghai Gao","doi":"10.3233/CBM-230175","DOIUrl":"10.3233/CBM-230175","url":null,"abstract":"Background: Epithelial-mesenchymal transition (EMT) is an important biological process by which malignant tumor cells to acquire migration and invasion abilities. This study explored the role of KLF5 in the EMT process of in cervical cancer cell lines.Objective: Krüpple-like factor 5 (KLF5) is a basic transcriptional factor that plays a key role in cell-cycle arrest and inhibition of apoptosis. However, the molecular mechanism by which KLF5 mediates the biological functions of cervical cancer cell lines has not been elucidated. Here, we focus on the potential function of ELF5 in regulating the EMT process in in vitro model of cervical cancer cell lines.Method: Western-blot and real-time quantitative PCR were used to detect the expression of EMT-related genes in HeLa cells. MTT assays, cell scratch and Transwell assays were used to assess HeLa cells proliferation and invasion capability. Using the bioinformatics tool JASPAR, we identified a high-scoring KLF5-like binding sequence in the SNAI1 gene promoter. Luciferase reporter assays was used to detect transcriptional activity for different SNAI1 promoter truncates.Result: After overexpressing the KLF5 gene in HeLa cells, KLF5 not only significantly inhibited the invasion and migration of HeLa cells, but also increased the expression of E-cadherin and decreased the expression of N-cadherin and MMP9. In addition, the mRNA expression of upstream regulators of E-cadherin, such as SNAI1, SLUG, ZEB1/2 and TWIST1 was also decreased. Furthermore, KLF5 inhibiting the expression of the SNAI1 gene via binding its promoter region, and the EMT of Hela cells was promoted after overexpression of the SNAI1 gene.Conclusion: These results indicate that KLF5 can downregulate the EMT process of HeLa cells by decreasing the expression of the SNAI1 gene, thereby inhibiting the migration and invasion of HeLa cervical cancer cells.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"231-243"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11191462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics comprehensive analysis reveals the predictive value of N6-methyladenosine- related genes in prognosis and immune escape of bladder cancer. 多组学综合分析揭示了N6-甲基腺苷相关基因在膀胱癌预后和免疫逃逸中的预测价值。

IF 2.2 4区医学

Cancer Biomarkers Pub Date : 2024-01-01 DOI: 10.3233/CBM-230286

Yang Liu, Zhongqi Pang, Jianshe Wang, Jinfeng Wang, Bo Ji, Yidan Xu, Jiaxin He, Lu Zhang, Yansong Han, Linkun Shen, Wanhai Xu, Minghua Ren

{"title":"Multi-omics comprehensive analysis reveals the predictive value of N6-methyladenosine- related genes in prognosis and immune escape of bladder cancer.","authors":"Yang Liu, Zhongqi Pang, Jianshe Wang, Jinfeng Wang, Bo Ji, Yidan Xu, Jiaxin He, Lu Zhang, Yansong Han, Linkun Shen, Wanhai Xu, Minghua Ren","doi":"10.3233/CBM-230286","DOIUrl":"10.3233/CBM-230286","url":null,"abstract":"Background: N6-methyladenosine (m6A) is the most frequent RNA modification in mammals, and its role in bladder cancer (BC) remains rarely revealed.Objective: To predict the value of m6A-related genes in prognosis and immunity in BC.Methods: We performed multiple omics analysis of 618 TCGA and GEO patients and used principal component analysis (PCA) to calculate the m6A score for BC patients.Results: We described the multiple omics status of 23 m6A methylation-related genes (MRGs), and four m6A clusters were identified, which showed significant differences in immune infiltration and biological pathways. Next, we intersected the differential genes among m6A clusters, and 11 survival-related genes were identified, which were used to calculate the m6A score for the patients. We found that the high-score (HS) group showed lower tumor mutation burden (TMB) and TP53 mutations and better prognosis than the low-score (LS) group. Lower immune infiltration, higher expression of PD-L1, PD-1, and CTLA4, and higher immune dysfunction and immune exclusion scores were identified in the LS group, suggesting a higher possibility of immune escape. Finally, the experimental verification shows that the m6A related genes, such as IGFBP1, plays an important role in the growth and metastasis of bladder cancer.Conclusions: These findings revealed the important roles of m6A MRGs in predicting prognosis, TMB status, TP53 mutation, immune functions and immunotherapeutic response in BC.","PeriodicalId":56320,"journal":{"name":"Cancer Biomarkers","volume":" ","pages":"79-94"},"PeriodicalIF":2.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11307005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140190399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0