{"title":"Positive Reinforcement Training Improves Behaviour and Welfare of Captive Indian Leopards (<i>Panthera pardus fusca</i>).","authors":"Akshaya Mohan Mane, Ajay Deshmukh, Brij Kishor Gupta","doi":"10.1080/10888705.2025.2521500","DOIUrl":"https://doi.org/10.1080/10888705.2025.2521500","url":null,"abstract":"<p><p>Rescued animals, including leopards, often experience stress from routine husbandry practices such as feeding, movement, and regrouping. Positive Reinforcement Training (PRT) has emerged as a valuable tool for reducing stress and enhancing the welfare of captive animals. This study investigated behavioral changes in rescued leopards during structured PRT. Using continuous focal animal sampling, data on individual and social behaviors were collected during five-minute sessions conducted three times weekly over a period of 12-16 weeks. Twelve leopards were observed across five phases: Baseline, Pre-Training, During Training, Post-Training, and Trained. Statistical analysis using the Kruskal-Wallis test revealed significant behavioral changes across phases. Leopards required an average of 2.1 sessions (SD ± 4.43 days, N = 29 observations) to retrieve rewards consistently. Structured training led to significant reductions in undesirable behaviors, including aggression (H(2) = 27.32, p < 0.0001) and stereotypical pacing (H(2) = 88.32, p < 0.0001), while engagement behaviors such as locomotion and exploration increased. Affiliative interactions with caregivers also rose, indicating stronger bonds. A decline in out-of-sight behavior showed improved focus, while increased alertness and maintenance behaviors suggested enhanced cognitive flexibility. Overall, PRT fosters positive behavioral changes, improves emotional well-being, and strengthens human-animal bonds.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-15"},"PeriodicalIF":1.4,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Value of a Statistical Life of a Cat: Owner Demographics and Management Practices Impacting Willingness to Pay for Welfare Measures.","authors":"Denise King, Panagiotis Tsigaris","doi":"10.1080/10888705.2025.2515856","DOIUrl":"https://doi.org/10.1080/10888705.2025.2515856","url":null,"abstract":"<p><p>This study estimates the Value of a Statistical Life of a cat (VSLC) in Kamloops, British Columbia, to inform animal welfare policies and community-level interventions. Using a contingent valuation survey, we assessed cat owners' willingness to pay (WTP) for control measures aimed at reducing the risk of premature death among outdoor cats. We compared WTP between indoor and outdoor cat owners and examined how demographic and attitudinal factors influenced WTP. The mean VSLC was approximately $8,000 among those willing to pay, and about $4,000 when non-payers were included. Notably, VSLC estimates were similar for both indoor and outdoor cat owners, suggesting that WTP reflects broader community concern for cat welfare rather than individual pet ownership circumstances. Regression results indicated that higher WTP was significantly associated with female gender, household income, concern for local wildlife, and support for cat licensing. These findings provide an economic basis for policies promoting responsible pet ownership and risk reduction for outdoor cats.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-13"},"PeriodicalIF":1.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144287377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Dairy Heifer Calves Submitted to Hot-Iron Branding: Behavior, Infrared Thermography, and Mechanical Nociceptive Threshold.","authors":"Jordana Augusta Rolim Zimmermann, Laís Baréa, Rogério Oliveira Rodrigues, Giovana Dantas","doi":"10.1080/10888705.2025.2515851","DOIUrl":"10.1080/10888705.2025.2515851","url":null,"abstract":"<p><p>In Brazil, hot-iron facial branding marks brucellosis-vaccinated heifers. Given its welfare impact, we assessed acute and chronic pain in branded dairy heifers. Twenty-four female calves, allocated into control (CG) and branded (BG) groups, were evaluated for acute pain through UCAPS scale and facial grimaces, and for chronic pain, from the 8th to the 24th week after branding, through pressure algometry. For inflammation, temperature in the lesion region (TLR) and in contralateral ocular region (ORT) was assessed. Animals were weighed during all the experiment. UCAPS indicated acute pain for at least 48h after branding (<i>p</i> < 0.05). Unit facial grimace activation occurred in BG (<i>p</i> < 0.05). TLR indicated active inflammation for at least 120h in BG (<i>p</i> < 0.05); ORT did not differ between groups (<i>p</i> > 0.05). Pressure algometry could not detect chronic pain in this study (<i>p</i> > 0.05). The weight gain was similar in both groups (<i>p</i> > 0.05). Although chronic pain has not been demonstrated, its occurrence cannot be excluded. Furthermore, hot-iron branding causes acute pain and compromises calf welfare, reinforcing the urgent need for less invasive identification methods.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-18"},"PeriodicalIF":1.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rachel Layton, David Beggs, Peter Mansell, Andrew Fisher, Daniel Layton, David T Williams, Kelly J Stanger
{"title":"Application of Minimally Invasive Physiological Monitoring Techniques in Pigs for Scientific Purposes.","authors":"Rachel Layton, David Beggs, Peter Mansell, Andrew Fisher, Daniel Layton, David T Williams, Kelly J Stanger","doi":"10.1080/10888705.2025.2516786","DOIUrl":"https://doi.org/10.1080/10888705.2025.2516786","url":null,"abstract":"<p><p>Wearable and implantable physiological monitors are designed for minimally invasive health assessment in companion animals. We aimed to establish proof-of-concept for the use of PetPace collar monitors and VetChip implantable monitoring devices in pigs. PetPace monitors were used to measure pulse rate, respiratory rate and heart rate variability in individually housed pigs. Group housed pigs were fitted with PetPace monitors to assess their feasibility for use in the presence of conspecifics, in addition to implantation with VetChip subcutaneous monitoring devices. PetPace monitors caused skin abrasion after 10 days of continual wear, but simple modifications allowed collars to be worn safely in individually housed pigs. Collars could not be worn unsupervised by group housed pigs due to damage by conspecifics. PetPace pulse and respiratory rate readouts showed a strong correlation with manual auscultations, and PetPace heart rate variability showed a very strong negative correlation with salivary corticosterone levels. VetChip temperature and pulse rate readouts were accurate in healthy pigs. However, the suitability of dorsal mid-scapula VetChip implantation is questionable due to wound breakdown in two out of three pigs.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-10"},"PeriodicalIF":1.4,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144250983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Individually Ventilated Cages Severely Attenuate Inter-Cage Ultrasonic Vocalizations in Rats.","authors":"K Jack Scott, David K Bilkey","doi":"10.1080/10888705.2025.2508270","DOIUrl":"https://doi.org/10.1080/10888705.2025.2508270","url":null,"abstract":"<p><p>Individually ventilated cages (IVCs) benefit rodent and researcher health but may limit animals' ability to communicate with cage-neighbours via ultrasonic vocalizations (USVs). Despite USVs' importance in rodent social behaviour, no previous research has investigated IVCs' impact on between-cage USV transmission. We recorded USV transmission between cages housing Sprague-Dawley rats in three conditions: in an open cage rack setting with microphone in an empty cage; from an open cage with a microphone outside an enclosed IVC; and between adjacent IVCs (with microphone in empty IVC). We compared the number and amplitude of USVs detected during 10-minute sessions across conditions. To verify results weren't due to differences in USV generation between cage types, we simultaneously recorded with two microphones, one inside an IVC and one in an adjacent enclosed IVC. Results showed USVs generated in open cages were clearly detectable in adjacent open cages but attenuated outside IVCs and virtually undetectable in adjacent IVCs. Since this loss of inter-cage USVs may affect animal behaviour, we recommend adjustments to cage design and handling procedures to mitigate these effects.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-10"},"PeriodicalIF":1.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physical Enrichment Enhances Growth in Artificially Reared Lambs.","authors":"Sarah Cowie","doi":"10.1080/10888705.2025.2500980","DOIUrl":"https://doi.org/10.1080/10888705.2025.2500980","url":null,"abstract":"<p><p>Precocious weaning is a common practise in large-scale sheep-dairy farming, despite causing distress and growth checks in lambs. We asked whether provision of physical enrichment objects would guard against growth checks during dairy lambs' first week in an artificial rearing system - arguably the most stressful period for artificially reared lambs. East Friesian Lacaune dairy ewe lambs aged approximately four to seven days were assigned to an Enrichment condition (<i>N</i> = 27) in which an object in the pen was changed daily, or a Control condition (<i>N</i> = 27) in which no object was available. Feeding and housing conditions were identical for both conditions. Lambs in the Enrichment condition experienced greater average daily weight gains (<u>m</u> = .33 kg; SD = .09) than Control-condition lambs (<u>m</u> = .21 kg; SD = .09) over the first week in the artificial rearing system (BF<sub>10</sub> = 3071.15). Thus, provision of physical enrichment may be particularly beneficial for lambs following precocious weaning.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-10"},"PeriodicalIF":1.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Towards Consensus: A Pilot Study in Enhancing Recognition of Equine Affective States with RaiSE (Recognizing Affective States in Equine).","authors":"A Wells, K Hiney, C M Brady, K A Anderson","doi":"10.1080/10888705.2025.2500979","DOIUrl":"https://doi.org/10.1080/10888705.2025.2500979","url":null,"abstract":"<p><p>The welfare of horses depends on the ability of their caretakers to accurately recognize and interpret their affective states. This study evaluates the effectiveness of RAiSE (Recognizing Affective States in Equine), an online educational course designed to enhance horse industry participants' skills in identifying equine emotions. The course content was developed using the Theory of Planned Behavior. The study involved 45 participants who completed pre- and post-surveys assessing their ability to interpret affective states from 20 video clips of horses. Results of the study showed that participants significantly improved in their interpretation of valence (<i>p</i> < 0.05). Findings also indicated a significant improvement in recognizing negative valence states post-course completion, with overall correct assessments increasing from 61% to 69% (<i>p</i> < 0.05). Participants rated the lessons \"Identifying Abnormal States\" and \"Horse-Human Interaction\" as particularly impactful. However, the course showed less effectiveness in improving recognition of positive valence states. The RAiSE course demonstrates potential for improving equine welfare through education, contributing to a more informed and humane approach to horse care. Future research should focus on improving the ability to assess positive affective states.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-14"},"PeriodicalIF":1.4,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Çağrı Melikşah Sakar, Yasin Ergiden, Nurgül Erdal, Yusuf Zengin, Adil Akın Aydın, Monica Battini
{"title":"Investigation on the Welfare of Angora Goats Using Animal and Resource-Based Indicators.","authors":"Çağrı Melikşah Sakar, Yasin Ergiden, Nurgül Erdal, Yusuf Zengin, Adil Akın Aydın, Monica Battini","doi":"10.1080/10888705.2025.2507046","DOIUrl":"https://doi.org/10.1080/10888705.2025.2507046","url":null,"abstract":"<p><p>This study aimed to assess the welfare levels of Angora goats in Ankara Province, Türkiye, using the Animal Needs Index (ANI) and Animal Welfare Indicators (AWIN) methods. The assessment was conducted on 28 farms, a total of 786 goats. By ANI score, the average was 46.8, with no significant difference based on farm size, as large (≥600), medium (300-599), and small (≤299) farms scored 45.6, 46.4, and 48.4 respectively. Using the AWIN Protocol, based on Body Condition Scores, 12.8% of goats were overweight and 11.1% underweight. Poor hair condition was observed in 14.1% of goats, while signs of thermal stress, abscesses, and fecal soiling were present in 1.5%, 1.1%, and 18.6% of goats, respectively; additionally, nasal discharge was detected in 21.2%, ocular discharge in 16.8%, overgrown claws in 18.2%, and udder asymmetry in 13.2% of the goats. The prevalence of dirty goats was observed in 43.6% and anemia (Famacha score 3-4-5) was found in 52.2% of goats. The study emphasizes the positive impact of smaller farm sizes on goat welfare, particularly in terms of locomotion and social interaction.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-14"},"PeriodicalIF":1.4,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144095936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bastian Herrera, Karen Ferhmann, Hernan Cañon Jones
{"title":"Knowledge, Attitudes, Perception and Willingness-To-Pay for Animal Welfare and Environmentally Friendly Certified Salmon Products in Chile: The Case of Bio Bio District.","authors":"Bastian Herrera, Karen Ferhmann, Hernan Cañon Jones","doi":"10.1080/10888705.2025.2507044","DOIUrl":"https://doi.org/10.1080/10888705.2025.2507044","url":null,"abstract":"<p><p>Aquaculture is expanding rapidly to meet growing protein demands, now providing over 50% of aquatic animal food. Salmon farming is especially important in Chile due to its favorable conditions and cost advantages. However, the industry faces challenges, including environmental sustainability and public perception. This study surveyed 396 people aged 18-65 in Chile's Bio Bio region about their knowledge and views on salmon farming and animal welfare. Results showed limited public understanding: 76% were unaware of information sources, and 52% were unsure of the industry's national role. Many viewed the industry as nontransparent, environmentally harmful, and poorly regulated, though its economic impact led to general acceptance. Knowledge of animal welfare was low-43% reported little or none-but support for better protections was high: 97% favored stronger welfare measures and 94% wanted more information. While 92% showed interest in welfare-certified salmon, only 21% were willing to pay up to 5% more. The study underscores the importance of public education and transparency to foster support for sustainable and welfare-conscious aquaculture.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-8"},"PeriodicalIF":1.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An Observational Study of Wood-Chewing in Mares and Their Foals Kept in Fenced Outdoor Paddocks with Variable Browsing Access.","authors":"A Bouquet, C Nicol, M Díez-León","doi":"10.1080/10888705.2025.2496496","DOIUrl":"https://doi.org/10.1080/10888705.2025.2496496","url":null,"abstract":"<p><p>The natural behaviour of wood-chewing (WC) in horses may, under certain conditions, become excessive or it may precede crib-biting . However, the potential causes of WC, including frustrated foraging motivation) or developmental exploratory or teething behavior are poorly understood. We studied WC in 31 mares and their foals, examining associations with browsing access, stocking density, foal sex and performance of other non-nutritive oral behaviors. Behaviors (counts/hour) were recorded by all-occurrences sampling during focal, continuous observations of grazing,resting and suckling for 20 × 10 min periods, for three days when foals were 3-5 months of age and kept at pasture with their mares. 17/31 mares and 28/31 foals exhibited WC, with foals performing more WC than mares (Mdn (IQR): foals: 0.95 (0.42,2.26); mares: 0.01 (0,0.19)). Colts exhibited significantly more WC than fillies (F<sub>1,13.07</sub> = 4.855, <i>p</i> = 0.046), possibly because of sex-based differences in exploratory tendencies. The amount of browsing access had a differential effect on mares and foals (F<sub>1,40.29</sub> = 3.720, <i>p</i> = 0.033). However, the amount of browsing access was partially confounded with foal age. Overall, we suggest that WC is associated more with developmental exploration than frustrated foraging access. Experimental work is needed to confirm our findings.</p>","PeriodicalId":56277,"journal":{"name":"Journal of Applied Animal Welfare Science","volume":" ","pages":"1-16"},"PeriodicalIF":1.4,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}