Journal of Microbiology Immunology and Infection最新文献

{"title":"Unveiling the dynamics of respiratory infections revealed by multiplex PCR testing during the COVID-19 pandemic in Taiwan, 2020–2023","authors":"Hung-Chieh Su ,&nbsp;Yu-Chang Chang ,&nbsp;Chih-Hao Chen ,&nbsp;Meng-Yu Cheng ,&nbsp;Wen-Hsin Hsih ,&nbsp;Yi-Jhen Chen ,&nbsp;Chia-Huei Chou ,&nbsp;Yu-Chao Lin ,&nbsp;Chiung-Tzu Hsiao ,&nbsp;Hong-Mo Shih ,&nbsp;Mao-Wang Ho ,&nbsp;Po-Ren Hsueh","doi":"10.1016/j.jmii.2024.08.003","DOIUrl":"10.1016/j.jmii.2024.08.003","url":null,"abstract":"<div><h3>Background</h3><div>The emergence of SARS-CoV-2 in late 2019 sparked the global COVID-19 pandemic, leading to varied vaccine policies worldwide. The evolving patterns of respiratory pathogens, aside from SARS-CoV-2, during the pandemic have had a significant impact on the development of vaccine strategies.</div></div><div><h3>Methods</h3><div>This study explores the landscape of respiratory pathogens, encompassing SARS-CoV-2, respiratory syncytial virus (RSV), and influenza viruses, through a retrospective analysis of data obtained from the BioFire Respiratory Panel 2.1 (RP 2.1) at China Medical University Hospital (Taichung, Taiwan) spanning from January 2020 to November 2023.</div></div><div><h3>Results</h3><div>Among the 7950 respiratory samples studied, pediatric cases exhibited higher positivity (64.9%, 2488/3835) and mixed detection rates (43.8%, 1090/2488) than adults. Annual mixed detection rates increased (27.9–48%). Prevalence analysis revealed diverse patterns across age groups, with higher rates in pediatrics. Notably, human rhinovirus/enterovirus predominated (48.1%). Mixed detection illustrated viral co-detections, notably with parainfluenza viruses and adenovirus. Government policies and pandemic dynamics influenced infection patterns, with RSV resurgence after May 2022. Age-specific RSV detection demonstrated a shift, influencing vaccine considerations. Amid global vaccine initiatives, RSV's increasing trend in adults warrants attention.</div></div><div><h3>Conclusions</h3><div>This comprehensive analysis emphasizes the importance of multiplex PCR testing in shaping targeted vaccination strategies during evolving respiratory pathogen landscapes.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"57 6","pages":"Pages 868-878"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142047604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of a human co-infected with seasonal influenza A (H3N2) virus and avian influenza A (H10N5) virus, China, December 2023","authors":"Zimin Xie,&nbsp;Fengxiang Xu,&nbsp;Rongmao Chen,&nbsp;Ming Liao,&nbsp;Manman Dai","doi":"10.1016/j.jmii.2024.07.008","DOIUrl":"10.1016/j.jmii.2024.07.008","url":null,"abstract":"","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"57 6","pages":"Pages 966-969"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141710876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictors of liver fibrosis changes assessed by paired liver biopsies in chronic hepatitis C patients treated with direct-acting antivirals","authors":"Ming-Han Hsieh ,&nbsp;Tzu-Yu Kao ,&nbsp;Ting-Hui Hsieh ,&nbsp;Chun-Chi Kao ,&nbsp;Cheng-Yuan Peng ,&nbsp;Hsueh-Chou Lai ,&nbsp;Hsing-Hung Cheng ,&nbsp;Mao-Wang Ho ,&nbsp;Chih-Yu Chi ,&nbsp;Jung-Ta Kao","doi":"10.1016/j.jmii.2024.08.005","DOIUrl":"10.1016/j.jmii.2024.08.005","url":null,"abstract":"<div><h3>Background/Purpose</h3><div>There are limited studies performing paired liver biopsies in chronic hepatitis C (CHC) patients treated with direct-acting antivirals (DAA). We aimed to investigate the predictors of liver fibrosis changes assessed by paired liver biopsies in these patients.</div></div><div><h3>Methods</h3><div>From March 2017 to March 2020, 113 CHC patients were prospectively enrolled to receive DAA therapy at our hospital. Paired liver biopsies were performed at baseline and 12 weeks after the end of treatment.</div></div><div><h3>Results</h3><div>Among the entire cohort, the rate of sustained virological response (SVR) was 100%. Four baseline variables independently predicted fibrosis regression, including age &lt;65 years [odds ratio (OR) = 2.725, p = 0.036], fibrosis stages (METAVIR scores) &lt; 3 (OR = 4.874, p = 0.040), hemoglobin levels ≥12.5 g/dL (OR = 3.538, p = 0.029), and platelet counts ≥160 10³/μL (OR = 2.958, p = 0.023). Besides, five independent predictors of fibrosis progression included baseline age ≥66 years (OR = 16.351, p = 0.024), body mass index (BMI) ≥26.5 kg/m² (OR = 21.666, p = 0.009), sofosbuvir/ribavirin use (OR = 29.465, p = 0.031), platelet counts &lt;119 10³/μL (OR = 33.739, p = 0.026), and the absence of alanine aminotransferase (ALT) levels declining from &gt;35 U/L at baseline to ≤35 U/L at 4 weeks after baseline (OR = 284.534, p = 0.026).</div></div><div><h3>Conclusion</h3><div>For DAA-treated CHC patients, those with baseline age &lt;65 years, fibrosis stages &lt;3, hemoglobin levels ≥12.5 g/dL, or platelet counts ≥160 10³/μL are more likely to attain fibrosis regression. There is a higher risk of fibrosis progression in those with baseline age ≥66 years, BMI ≥26.5 kg/m², sofosbuvir/ribavirin use, platelet counts &lt;119 10³/μL, or the absence of early ALT normalization at 4 weeks after baseline.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"57 6","pages":"Pages 840-853"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequential use of capsular typing and whole-genome sequencing-based analysis for transmission of carbapenem-resistant Acinetobacter baumannii in a tertiary medical center","authors":"Yi-An Way ,&nbsp;Chong-Wei Huang ,&nbsp;Wei-Chao Liao ,&nbsp;Shiao-Wen Li ,&nbsp;Ruei-Lin Chiang ,&nbsp;En-Wei Hsing ,&nbsp;Yi-Jiun Pan ,&nbsp;Shian-Sen Shie ,&nbsp;Yu-Chia Hsieh","doi":"10.1016/j.jmii.2024.08.014","DOIUrl":"10.1016/j.jmii.2024.08.014","url":null,"abstract":"<div><h3>Background</h3><div>During the COVID-19 pandemic, there has been an increasing trend in healthcare-associated infections (HAIs) caused by carbapenem-resistant <em>Acinetobacter baumannii</em> (CRAB), posting a global public health concern. The heightened sensitivity of whole-genome sequencing (WGS) renders it an optimal and potent tool for monitoring outbreaks and tracing the transmission routes of nosocomial pathogens.</div></div><div><h3>Method</h3><div>We collected CRAB isolates from March 1, 2023, to April 6, 2023 in Chang Gung Memorial Hospital Lin Kou branch, a tertiary medical center in northern Taiwan. Any two or more isolates with the same identifiable capsular K-locus (KL) types were selected, and analyzed via WGS to identify putative transmission clusters, combined with epidemiologic and retrospective analysis on medical records to confirm risk factors and hidden transmission chains.</div></div><div><h3>Result</h3><div>A total of 48 non-redundant CRAB isolates were collected, belonging to ST2 of Pasteur MLST scheme and identifiable KL types of KL2, KL3, KL9, KL10, KL22, KL52. Excluding the KL types that was only found in 1 case, KL2 (n = 9, 22.5 %), KL3 (n = 24, 60 %), KL9 (n = 3, 7.5 %), and KL10 (n = 4, 10 %) were selected for further WGS analysis. Four distinct transmission clusters comprised of 2, 3, 10, and 23 cases were identified on a basis of phylogenetic status. 12 probable transmission chains were revealed, and 2 hidden transmission routes can be speculated.</div></div><div><h3>Conclusion</h3><div>This study referred to some hidden transmission chains that may be missed from traditional surveillance measures. Despite its low prevalence and high cost currently, implementing WGS could be a efficient, prompt, and unequivocal option for future MDRO infection control.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"57 6","pages":"Pages 916-925"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptococcus canis infection in Taiwan","authors":"Pei-Yun Tsai,&nbsp;Wan-Hsuan Hsu,&nbsp;Yu-Hsin Chiu,&nbsp;Chih-Cheng Lai,&nbsp;Hung-Jen Tang","doi":"10.1016/j.jmii.2024.09.004","DOIUrl":"10.1016/j.jmii.2024.09.004","url":null,"abstract":"","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"57 6","pages":"Pages 970-971"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactate dehydrogenase-1 may play a key role in the brain energy disturbance caused by cryptococcal meningitis","authors":"Qingdong Zhu ,&nbsp;Qian Long ,&nbsp;Cailing Wei ,&nbsp;Jieling Chen ,&nbsp;Lanwei Nong ,&nbsp;Jianglong Qin ,&nbsp;Zhizhong Huang ,&nbsp;Yanqing Zheng ,&nbsp;Sijun Li","doi":"10.1016/j.jmii.2024.08.009","DOIUrl":"10.1016/j.jmii.2024.08.009","url":null,"abstract":"<div><h3>Background</h3><div>Cryptococcal meningitis (CM) may affect the conversion of lactate to pyruvate in the brain, resulting in abnormal levels of adenosine triphosphate (ATP) throughout the brain. Lactate conversion to pyruvate is mainly caused by lactic dehydrogenase 1 (LDH1), which is composed of four LDHB subunits. However, the underlying mechanism of LDH1 in CM remains unclear.</div></div><div><h3>Methods</h3><div>Cerebrospinal fluid (CSF) from 17 patients was collected, including eight patients with non-infectious diseases of the central nervous system and nine patients with CM. Based on clinical data and laboratory reports, data regarding intracranial pressure, CSF white cell counts, lactate dehydrogenase (LDH), adenosine deaminase, glucose, protein, and chloridion were collected. Meanwhile, LDH1, LDH5, lactate, pyruvate, and ATP levels were detected in CSF. Whereafter, the levels of lactate, pyruvate, ATP, and the amplitude and frequency of action potentials in the neurons with low expression of LDHB were explored.</div></div><div><h3>Results</h3><div>Intracranial pressure and white cell count in CSF were significantly increased in patients with CM. In patients with CM, the LDH1, pyruvate, and ATP levels in the CSF were significantly decreased, and the levels of lactate were found to be increased. Furthermore, pyruvate and ATP levels were decreased, while lactate was increased in the neurons with low expression of LDHB. The amplitude and frequency of APs in the neurons with low expression of LDHB were significantly decreased.</div></div><div><h3>Conclusion</h3><div>Reduced levels of LDH1 in the brain of patients with CM may lead to increased lactate levels, decreased pyruvate and ATP levels, and negatively affect neuronal activity.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"57 6","pages":"Pages 887-895"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic potential of combining plasma biomarkers of tissue damage and inflammation in pediatric TB","authors":"Andrea López-Suárez ,&nbsp;Mar Santos-Sebastián ,&nbsp;Alicia Hernanz-Lobo ,&nbsp;Elena Rincón-López ,&nbsp;David Aguilera-Alonso ,&nbsp;Jesús Saavedra-Lozano ,&nbsp;María Jesús Ruiz Serrano ,&nbsp;Ángel Hernández-Bartolomé ,&nbsp;Luz María Medrano de Dios ,&nbsp;José Luis Jiménez Fuentes ,&nbsp;María Luisa Navarro ,&nbsp;Marc Tebruegge ,&nbsp;Begoña Santiago-García","doi":"10.1016/j.jmii.2024.07.011","DOIUrl":"10.1016/j.jmii.2024.07.011","url":null,"abstract":"<div><h3>Introduction</h3><div>Immune-based diagnostic tests for tuberculosis (TB) have suboptimal sensitivity in children and cannot differentiate between latent infection (LTBI) and active disease. This study evaluated the diagnostic potential of a broad range of biomarkers of tissue damage and inflammation in unstimulated plasma in children.</div></div><div><h3>Methods</h3><div>We analyzed 17 biomarkers in 15 non–<em>M. tuberculosis</em> (MTB)-infected controls and 33 children with TB infection (LTBI, n = 8; probable TB, n = 19; confirmed TB, n = 6). Biomarker concentrations were measured using a Luminex magnetic bead–based platform and multiplex sandwich immunoassays. Concentrations, correlations and diagnostic accuracy assessments were conducted among patient groups.</div></div><div><h3>Results</h3><div>Confirmed TB cases had significantly higher concentrations of IFN-γ and IL-2 and higher IFN-γ/MCP-1 and IL-2/MCP-1 ratios compared to LTBI and non–MTB-infected children. Among children with confirmed TB, there was a strong correlation between IFN-γ and IL-10 (r = 0.95; p &lt; 0.001) and a significant correlation between IL-2 and IL-1ra (r = 0.92), IL-21 (r = 0.91), MCP-3 (r = 0.84), and MMP-1 (r = 0.85). The IFN-γ/MCP-1 ratio was the most accurate biomarker combination for differentiating between MTB-infected and non–MTB-infected children (AUC, 0.82; sensitivity, 87.9%; specificity, 66.6%; p &lt; 0.001) and between active TB and non–MTB-infected children (AUC 0.82; sensitivity 88.0%; specificity 60.0%; p &lt; 0.001). None of the biomarkers investigated were able to discriminate between LTBI and active TB.</div></div><div><h3>Conclusion</h3><div>Our data suggest that combining the analyses of multiple biomarkers in plasma has the potential to enhance diagnosis of TB in children and, thus, warrants additional investigation. In particular, the diagnostic potential of IFN-γ/MCP-1 ratios should be further explored in larger pediatric cohorts.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"57 6","pages":"Pages 937-946"},"PeriodicalIF":4.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142251591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and genomic changes of recurrent Methicillin‐Resistant Staphylococcus aureus bacteremia","authors":"Tu-Hsuan Chang ,&nbsp;Hung-Jen Tang ,&nbsp;Chi-Chung Chen ,&nbsp;Chih-Jung Chen","doi":"10.1016/j.jmii.2024.11.008","DOIUrl":"10.1016/j.jmii.2024.11.008","url":null,"abstract":"<div><h3>Background</h3><div>Recurrent or persistent methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) bacteremia presents significant clinical challenges. Comprehensive genomic-scale studies on the genetic changes in MRSA that correspond to refractory bacteremia are lacking.</div></div><div><h3>Method</h3><div>From 2011 to 2019, MRSA blood isolates were collected from patients with persistent or recurrent bacteremia at a teaching hospital in southern Taiwan. Whole-genome sequencing (WGS) captured the genomic changes in strains responsible for refractory bacteremia, and the altered susceptibilities to specific antimicrobial agents were assessed through measurements of minimal inhibitory concentrations (MICs).</div></div><div><h3>Result</h3><div>A total of 35 MRSA blood isolates from 15 patients with recurrent or persistent bacteremia were analyzed. Reduced susceptibilities to at least one anti-MRSA agent developed in strains from seven (46.7 %) patients. Of them, a non-synonymous mutation on a global regulator <em>mgrA</em> was associated with reduced daptomycin susceptibility, while an increase in vancomycin MIC was linked to mutations in genes encoding LCP family protein. A 16-fold increase in MIC to fusidic acid was connected to a mutation in the elongation factor G. These recurrent strains commonly exhibited a loss or acquisition of adhesion genes that were involved in biofilm formation, including <em>fnbA</em>, <em>fnbB</em>, and <em>sdrD</em>, and <em>easG</em> series genes of type VII secretion system.</div></div><div><h3>Conclusion</h3><div>Changes in the susceptibility of successive strains to common anti-MRSA agents were frequently observed in recurrent MRSA bacteremia. These changes were linked to modifications in genes of regulatory cascade, peptidoglycan binding, adhesion, and type VII secretion system.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"58 2","pages":"Pages 251-257"},"PeriodicalIF":4.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schistosoma mansoni soluble egg antigen suppresses colorectal cancer growth in vitro and in vivo","authors":"Ho Yin Pekkle Lam ,&nbsp;Ting-Ruei Liang ,&nbsp;Shinn-Jong Jiang ,&nbsp;Shih-Yi Peng","doi":"10.1016/j.jmii.2024.11.009","DOIUrl":"10.1016/j.jmii.2024.11.009","url":null,"abstract":"<div><h3>Background</h3><div>Colorectal cancer (CRC) is the third most common malignant disease around the world. Because the hosts’ immunity plays a great part in regulating tumor cells' growth and progression, immunotherapies have therefore aroused great interest in treating cancers. Currently, scientists have investigated the use of <em>Schistosoma</em>-derived soluble egg antigens (SEA), which is known as a strong immune modulator, in treating a series of immune-related diseases.</div></div><div><h3>Methods</h3><div>In this study, we investigated the anti-tumor effect of SEA against CRC using <em>in vitro</em> cell lines, HCT-116 and DLD-1, as well as <em>in vivo</em> mouse xenograft model. Approaches such as migration assay, invasion assay, and western blotting were done to analyze the anti-tumor effect of SEA. Furthermore, qRT-PCR and ELISA were performed to identify the immune profile of SEA-treated cells as well as SEA-treated xenograft mice.</div></div><div><h3>Results</h3><div><em>In vitro</em> studies suggested that SEA can dose-dependently inhibit the growth and progression of HCT-116 and DLD-1 cells. This inhibition was accompanied by a reduction of epithelial-mesenchymal transition (EMT), inflammasome inactivation, and apoptosis. SEA also downregulated the expression of IL-4 and IL-10 in the CRC cells, which may be the reason why their growth and progression were suppressed. <em>In vivo</em> studies showed a similar beneficial effect of SEA, as local administration of 25 μg SEA significantly inhibits tumor cell growth. SEA treatment also shifts the host's immunity from a pro-tumorigenic response to an anti-tumor response.</div></div><div><h3>Conclusion</h3><div>In conclusion, SEA may provide a beneficial effect against CRC, and further investigation may give promise in CRC treatment.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"58 2","pages":"Pages 241-250"},"PeriodicalIF":4.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of a Sepsityper® kit and in-house membrane filtration methods for rapidly diagnosing positive blood cultures via MALDI‒TOF MS","authors":"Lee Tai-Fen ,&nbsp;Wan Tsai-Wen ,&nbsp;Hsu Wei-Yu ,&nbsp;Chen Xiang-Jun ,&nbsp;Chiu Hao-Chieh ,&nbsp;Huang Yu-Tsung","doi":"10.1016/j.jmii.2024.11.007","DOIUrl":"10.1016/j.jmii.2024.11.007","url":null,"abstract":"<div><h3>Background</h3><div>Rapidly identifying pathogens and determining their antimicrobial susceptibilities using samples directly from flagged blood culture bottles pose significant challenges for clinical laboratories. Thus, a cost-effective and efficient sample-processing method is urgently needed to address this issue. To fulfill this need, we developed a novel protocol to rapidly identify pathogens and determine their antimicrobial susceptibilities using samples directly from blood culture bottles.</div></div><div><h3>Methods</h3><div>Samples were either processed by the Sepsityper kit or our in-house methods. In our approach, we processed the samples using either a nonionic surfactant (Triton X-100) or a NaOH-sodium dodecyl sulfate (SDS) solution, followed by membrane filtration (MF) and centrifugation. Subsequently, the samples were analyzed using MALDI-TOF mass spectrometry (MS) for identification and the Vitek® 2 for antimicrobial susceptibility determination.</div></div><div><h3>Results</h3><div>In this study, 122 clinical blood culture samples were analyzed, and our MF protocol displayed enhanced accuracy in identifying gram-positive organisms (n = 58) and gram-negative bacilli (n = 64) compared to the Sepsityper method. In particular, the Triton-MF and SDS-MF techniques outperformed Sepsityper in identifying gram-negative bacilli, with accuracy rates of 92.2 %, 85.9 %, and 78.1 %, respectively. Notably, both the Triton-MF and SDS-MF methods exhibited high categorical agreement (CA) for antimicrobial susceptibility testing (AST) for carbapenem against Enterobacterales, with CAs of 100 % and 98.7 %, respectively. Additionally, both methods exhibited a perfect CA and essential agreement of 100 % for <em>Enterococcus faecium</em> AST for vancomycin.</div></div><div><h3>Conclusion</h3><div>These findings strongly indicate that our MF methods have the potential to streamline the identification and AST of bacteria in positive blood cultures.</div></div>","PeriodicalId":56117,"journal":{"name":"Journal of Microbiology Immunology and Infection","volume":"58 2","pages":"Pages 265-271"},"PeriodicalIF":4.5,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}