Rice Science最新文献

{"title":"RISE Method Based on Rare Allele Infusion and Sanger Sequencing Estimation: A Simple, Cheap, and Efficient Method for Detecting Transgene Copy Number in Rice","authors":"","doi":"10.1016/j.rsci.2024.05.001","DOIUrl":"10.1016/j.rsci.2024.05.001","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 499-502"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141395974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice Cultivation under Film Mulching Can Improve Soil Environment and Be Beneficial for Rice Production in China","authors":"Youliang Zhang,&nbsp;Kaican Zhu,&nbsp;Yongqi Tang,&nbsp;Shaoyuan Feng","doi":"10.1016/j.rsci.2024.06.009","DOIUrl":"10.1016/j.rsci.2024.06.009","url":null,"abstract":"<div><div>Rice cultivation under film mulching is an integrated management technology that can conserve water, increase soil temperature, improve yield, and enhance water and nitrogen use efficiencies. Despite these advantages, the system does have its drawbacks, such as soil organic matter reduction and microplastic pollution, which impede the widespread adoption of film mulching cultivation in China. Nonetheless, the advent of degradable film, controlled-release fertilizer, organic fertilizer, and film mulching machinery is promoting the development of rice film mulching cultivation. This review outlines the impact of rice cultivation under film mulching on soil moisture, soil temperature, soil fertility, greenhouse gas emissions, weed control, and disease and pest management. It also elucidates the mechanism of changes in rice growth, yield and quality, water use efficiency, and nitrogen use efficiency. This paper incorporates a review of published research articles and discusses some uncertainties and shortcomings associated with rice cultivation under film mulching. Consequently, prospective research directions for the technology of rice film mulching cultivation are outlined, and recommendations for future research into rice cultivation under film mulching are proposed.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 545-555"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bulked Segregant RNA-Seq Analysis of Pollinated Pistils Reveals Genes Influencing Spikelet Fertility in Rice","authors":"Kanokwan Kaewmungkun ,&nbsp;Keasinee Tongmark ,&nbsp;Sriprapai Chakhonkaen ,&nbsp;Numphet Sangarwut ,&nbsp;Theerachai Thanananta ,&nbsp;Amorntip Muangprom","doi":"10.1016/j.rsci.2024.06.001","DOIUrl":"10.1016/j.rsci.2024.06.001","url":null,"abstract":"<div><div>Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F_5:6 generation plants derived from inter-subspecific crosses (Nipponbare × KDML105) with low (LS) and high seed-setting rates (HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4‒5 h post-pollination, compared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes (DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS- specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interconversions, and flavonoid biosynthesis. Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks. Compared with LS pistils, enzyme genes controlling pectin degradation, such as <em>OsPME35</em> and <em>OsPLL9</em>, showed similar expression patterns, with higher levels in HS pistils pre-pollination. <em>Os02g0467600</em>, similar to cinnamate 4-hydroxylase gene (<em>CYP73</em>), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that <em>OsPME35</em>, <em>OsPLL9</em>, and <em>Os02g0467600</em> contribute to prezygotic isolation by potentially modifying the stigma cell wall (<em>OsPME35</em> and <em>OsPLL9</em>) and controlling later processes such as pollen-stigma adhesion (<em>Os02g0467600</em>) genes. Furthermore, several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately contributing to the development of high-yielding rice varieties.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 556-571"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochar Decreases Soil Cadmium (Cd) Availability and Regulates Expression Levels of Cd Uptake/Transport-Related Genes to Reduce Cd Translocation in Rice","authors":"","doi":"10.1016/j.rsci.2024.04.004","DOIUrl":"10.1016/j.rsci.2024.04.004","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 494-498"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140773589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next Generation Nutrition: Genomic and Molecular Breeding Innovations for Iron and Zinc Biofortification in Rice","authors":"","doi":"10.1016/j.rsci.2024.04.008","DOIUrl":"10.1016/j.rsci.2024.04.008","url":null,"abstract":"<div><div>Global efforts to address malnutrition and hidden hunger, particularly prevalent in low- and middle-income countries, have intensified, with a focus on enhancing the nutritional content of staple crops like rice. Despite serving as a staple for over half of the world’s population, rice falls short in meeting daily nutritional requirements, especially for iron (Fe) and zinc (Zn). Genetic resources, such as wild rice species and specific rice varieties, offer promising avenues for enhancing Fe and Zn content. Additionally, molecular breeding approaches have identified key genes and loci associated with Fe and Zn accumulation in rice grains. This review explores the genetic resources and molecular mechanisms underlying Fe and Zn accumulation in rice grains. The functional genomics involved in Fe uptake, transport, and distribution in rice plants have revealed key genes such as <em>OsFRO1</em>, <em>OsIRT1</em>, and <em>OsNAS3</em>. Similarly, genes associated with Zn uptake and translocation, including <em>OsZIP11</em> and <em>OsNRAMP1</em>, have been identified. Transgenic approaches, leveraging transporter gene families and genome editing technologies, offer promising avenues for enhancing Fe and Zn content in rice grains. Moreover, strategies for reducing phytic acid (PA) content, a known inhibitor of mineral bioavailability, have been explored, including the identification of low-PA mutants and natural variants. The integration of genomic information, including whole-genome resequencing and pan-genome analyses, provides valuable insights into the genetic basis of micronutrient traits and facilitates targeted breeding efforts. Functional genomics studies have elucidated the molecular mechanisms underlying Fe uptake and translocation in rice. Furthermore, transgenic and genome editing techniques have shown promise in enhancing Fe and Zn content in rice grains through the manipulation of key transporter genes. Overall, the integration of multi-omics approaches holds significant promise for addressing global malnutrition and hidden hunger by enhancing the nutritional quality of rice, thereby contributing to improved food and nutritional security worldwide.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 526-544"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141033035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating Key Phenological Dates of Multiple Rice Accessions Using Unmanned Aerial Vehicle-Based Plant Height Dynamics for Breeding","authors":"","doi":"10.1016/j.rsci.2024.04.007","DOIUrl":"10.1016/j.rsci.2024.04.007","url":null,"abstract":"<div><div>Efficient and high-quality estimation of key phenological dates in rice is of great significance in breeding work. Plant height (PH) dynamics are valuable for estimating phenological dates. However, research on estimating the key phenological dates of multiple rice accessions based on PH dynamics has been limited. In 2022, field traits were collected using unmanned aerial vehicle (UAV)-based images across 435 plots, including 364 rice varieties. PH, dates of initial heading (IH) and full heading (FH), and panicle initiation (PI), and growth period after transplanting (GPAT) were collected during the rice growth stage. PHs were extracted using a digital surface model (DSM) and fitted using Fourier and logistic models. Machine learning algorithms, including multiple linear regression, random forest (RF), support vector regression, least absolute shrinkage and selection operator, and elastic net regression, were employed to estimate phenological dates. Results indicated that the optimal percentile of the DSM for extracting rice PH was the 95th (<em>R</em>² = 0.934, RMSE = 0.056 m). The Fourier model provided a better fit for PH dynamics compared with the logistic models. Additionally, curve features (CF) and GPAT were significantly associated with PI, IH, and FH. The combination of CF and GPAT outperformed the use of CF alone, with RF demonstrating the best performance among the algorithms. Specifically, the combination of CF extracted from the logistic models, GPAT, and RF yielded the best performance for estimating PI (<em>R</em>² = 0.834, RMSE = 4.344 d), IH (<em>R</em>² = 0.877, RMSE = 2.721 d), and FH (<em>R</em>² = 0.883, RMSE = 2.694 d). Overall, UAV-based rice PH dynamics combined with machine learning effectively estimated the key phenological dates of multiple rice accessions, providing a novel approach for investigating key phenological dates in breeding work.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 617-628"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141050138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Machine Vision-Based Algorithm for Counting and Discriminating Filled and Unfilled Paddy Rice in Overlapping Mode","authors":"","doi":"10.1016/j.rsci.2024.04.001","DOIUrl":"10.1016/j.rsci.2024.04.001","url":null,"abstract":"","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 503-506"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140756556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Understanding Cadmium Stress and Breeding of Cadmium-Tolerant Crops","authors":"Liang Liang,&nbsp;Wang Chenchang,&nbsp;Chen Tao","doi":"10.1016/j.rsci.2024.06.006","DOIUrl":"10.1016/j.rsci.2024.06.006","url":null,"abstract":"<div><div>Cadmium (Cd) pollution has emerged as a critical global environmental concern, due to its significant toxicity, environmental persistence, and the pervasiveness of contamination. Significantly, the bioaccumulation of Cd in agricultural crops constitutes a primary vector for its entry into the human diet. This issue warrants urgent attention from both the scientific community and policymakers to develop and implement effective mitigation strategies. This review delves into the physiological impacts of Cd stress on plants, including the suppression of photosynthetic activity, amplification of oxidative stress, and disruptions in mineral nutrient homeostasis. Additionally, the resistance mechanisms deployed by plants in response to Cd stress have been explored, and the prospective contributions of molecular breeding strategies in augmenting crop tolerance to Cd and minimizing its bioaccumulation have been assessed. By integrating and analyzing these findings, we seek to inform future research trajectories and proffer strategic approaches to enhance agricultural sustainability, safeguard human health, and protect environmental integrity.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 507-525"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142425062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative Effects of Paclobutrazol via Physio-Biochemical and Molecular Manifestation in Rice under Water Deficit Stress","authors":"","doi":"10.1016/j.rsci.2024.03.004","DOIUrl":"10.1016/j.rsci.2024.03.004","url":null,"abstract":"<div><div>To comprehensively explore the physio-biochemical and molecular changes of paclobutrazol (PBZ) at the ideal dose under water deficit stress (WDS) conditions, we investigated the effects of 100 mg/kg PBZ applied via drenching on various physio-biochemical and molecular parameters in three rice varieties (N22, IR64, and IR64 DTY1.1) under both mild [75%‒80% relative water content (RWC)] and severe (60%‒65% RWC) WDS conditions. The results showed that PBZ treatment positively influenced the physio-biochemical parameters, significantly increasing dry matter (16.27%‒61.91%), RWC (6.48%‒ 16.34%), membrane stability index (4.37%‒10.35%), and total chlorophyll content (8.97%‒29.09%) in the rice varieties under both mild and severe WDS. Moreover, PBZ treatment reduced drought susceptibility (0.83‒0.95) and enhanced drought tolerance efficiency (60.92%‒86.78%), indicating its potential as a stress-mitigating agent. Global methylation analysis revealed changes in DNA methylation patterns, indicating the regulatory influence of PBZ on gene expression. The expression analysis of genes involved in the diversification of geranylgeranyl pyrophosphate towards the biosynthesis of abscisic acid, gibberellin acid, and chlorophyll showed alterations in their expression levels, suggesting the involvement of PBZ in the isoprenoid pathway. Overall, this study provides valuable insights into the potential mechanisms by which PBZ modulates physiological and molecular responses in rice plants under WDS. The findings highlight the importance of PBZ as a promising agent for enhancing drought tolerance in rice and offer valuable information for future research in crop stress management.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 603-616"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141033981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Appropriate Supply of Ammonium Nitrogen and Ammonium Nitrate Reduces Cadmium Content in Rice Seedlings by Inhibiting Cadmium Uptake and Transport","authors":"","doi":"10.1016/j.rsci.2024.02.007","DOIUrl":"10.1016/j.rsci.2024.02.007","url":null,"abstract":"<div><div>Reasonable nitrogen (N) application is a promising strategy for reducing crop cadmium (Cd) toxicity. However, the specific form of N and the required amount that affect Cd tolerance and accumulation in rice remain unclear. This study explored the influence of different N-fertilizer forms (NH₄NO₃, NH₄Cl, and KNO₃) and dosages on Cd tolerance and uptake in Cd-stressed N-sensitive and N-insensitive <em>indica</em> rice accessions. The results indicated that the Cd tolerance of N-sensitive <em>indica</em> accessions is more robust than that of N-insensitive ones. Furthermore, the shoot Cd content and Cd translocation rate in both N-sensitive and N-insensitive <em>indica</em> accessions decreased with an appropriate supply of NH₄NO₃ and NH₄Cl, whereas they were comparable or slightly increased with increased KNO₃. Unfortunately, we did not find significant and regular differences in Cd accumulation or translocation between N-sensitive and N-insensitive rice accessions. Consistent with the reduction of shoot Cd content, the addition of NH₄NO₃ and NH₄Cl also inhibited the instantaneous root Cd²⁺ uptake. The expression changes of Cd transport-related genes under different N forms and dosages suggested that the decreased shoot Cd content, caused by the increased supply of NH₄NO₃ and NH₄Cl, is likely achieved by reducing the transcription of <em>OsNRAMP1</em> and <em>OsIRT1</em>. In summary, our findings reveal that an appropriate supply of NH₄NO₃ and NH₄Cl could reduce Cd uptake and transport in rice seedlings, suggesting that rational N management could reduce the Cd risk in rice production.</div></div>","PeriodicalId":56069,"journal":{"name":"Rice Science","volume":"31 5","pages":"Pages 587-602"},"PeriodicalIF":5.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139876064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}