Trends in Environmental Analytical Chemistry最新文献

{"title":"CRISPR-powered biosensors for metal ion monitoring: From molecular recognition to environmental applications","authors":"Prabir Kumar Kulabhusan,&nbsp;David Septian Sumanto Marpaung,&nbsp;Guozhen Liu","doi":"10.1016/j.teac.2026.e00318","DOIUrl":"10.1016/j.teac.2026.e00318","url":null,"abstract":"<div><div>Heavy metals in the environment are harmful both to human beings and the environment. In the past, heavy metal contamination was detected using traditional analytical methods that were time-consuming, costly, and required complex equipment. In recent years, several different types of biosensors based upon the programmable use of nucleic acids have been developed. The focus of this review is to cover the architecture, principles of operation, and performance characteristics of CRISPR-based biosensors for detection of heavy metals. CRISPR-Cas12 and Cas13 nucleases enable programmable biosensors through collateral trans-cleavage of single-stranded DNA and RNA, respectively. The review further explores the conversion of specific heavy metal binding events into nucleic acid signals and their subsequent integration with CRISPR based molecular system. In addition, many different types of signal transduction modalities), and the use of nanomaterials for signal enhancement for heavy metals are also analysed. Presently, the available technologies demonstrates good recovery rates and comparable performance to ICP-MS for metal detection in complex matrix. Nonetheless, substantial challenges persists, including matrix interference from organic matter and competing ions, component stability and scalability, and regulatory standardisation. Future efforts are likely to include the development of portable devices integrated with smartphones, multiplex detection, microfluidic lab-on-a-chip integration, and the implementation of artificial intelligence to assist in data analysis. This article presents a thorough roadmap for converting laboratory advances into dependable technology capable of providing comprehensive monitoring of heavy metals for use in the field while stressing the need for ongoing optimisation with real-world sample matrices.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"51 ","pages":"Article e00318"},"PeriodicalIF":12.2,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148934483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of disperser solvent-free dispersive liquid–liquid microextraction for environmental organic pollutants in environmental samples: Recent advances, green strategies, and future perspectives","authors":"Wael I. Mortada","doi":"10.1016/j.teac.2026.e00309","DOIUrl":"10.1016/j.teac.2026.e00309","url":null,"abstract":"<div><div>Dispersive liquid–liquid microextraction (DLLME) has progressed into a simple, efficient, rapid, and miniaturized sample preparation procedure for analysis of environmental organic pollutants in different matrices. This review emphasizes recent advancements that enhance the performance and sustainability of DLLME for environmental analysis by eliminating disperser solvents in the analytical procedure. The article discusses advances in disperser solvent-free DLLME (DSF-DLLME), including ultrasound-, vortex-, air-, and effervescence-assisted approaches, which significantly improve mass transfer and extraction kinetics and improve the environmental sustainability of the procedure. The broad utilization of ecofriendly extraction solvents is also addressed, with emphasis on ionic liquids, low-density organic solvents, and deep eutectic solvents as alternatives to conventional solvents. The review also explores hybrid automated DLLME systems, covering their conceptual design, instrumentation, and main advantages, alongside current limitations. Finally, future directions focus on automation, miniaturization, green chemistry principles, and integration with advanced analytical techniques. Overall, these innovations position DSF-DLLME as a promising and practical technique for modern analytical applications in environmental analysis.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"50 ","pages":"Article e00309"},"PeriodicalIF":13.4,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148183918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pesticide contamination in apicultural products: An updated and comprehensive review of analytical methods, occurrence, and safety concerns","authors":"Adrián Fuente-Ballesteros ,&nbsp;Maj Smerkol ,&nbsp;Anton Gradišek ,&nbsp;Artur Sarmento ,&nbsp;Julie Fourrier ,&nbsp;Mila Arapcheska ,&nbsp;Zehra Hajrulai-Musliu ,&nbsp;Filip Franeta ,&nbsp;Željko Milovac ,&nbsp;Sonja Gvozdenac ,&nbsp;Nesrin İçli ,&nbsp;Harun Kurtagić ,&nbsp;Ina Pasho ,&nbsp;Elena Zioga ,&nbsp;Beatriz I. Vazquez ,&nbsp;Damir Pavliček ,&nbsp;Rosa Busquets ,&nbsp;Jelena Ciric ,&nbsp;Nurinisa Esenbuga ,&nbsp;Miriam Cavaco ,&nbsp;Iveta Pugajeva","doi":"10.1016/j.teac.2026.e00300","DOIUrl":"10.1016/j.teac.2026.e00300","url":null,"abstract":"<div><div>Honeybees and their products integrate landscape-level chemical exposure, making apicultural matrices valuable bioindicators for both food safety and environmental monitoring. This review summarizes current knowledge on pesticide residues in honey, pollen, beebread, beeswax, royal jelly, and propolis from 2019 to 2024, with an overview of analytical methodologies used in their determination. Multi-residue methods remain dominated by Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) extraction combined with liquid and gas chromatography coupled to tandem mass spectrometry, while high-resolution MS enables broader screening. Highly polar pesticides, particularly glyphosate and its metabolites, require specialised single-residue approaches, such as the Quick Polar Pesticides (QuPPe) method and ion chromatography–high-resolution mass spectrometry (IC-HRMS). Co-occurrence patterns frequently involve mixtures of neonicotinoids, acaricides, and fungicides, reflecting combined agricultural and in-hive treatments. Regarding matrices, honey typically shows insecticide and acaricide residues, pollen concentrates fungicides and insecticides as the main exposure route, and beeswax acts as a long-term sink for lipophilic compounds; royal jelly generally exhibits the lowest contamination levels. Although exceedances of Maximum Residue Limits in honey remain uncommon in European monitoring programs, the presence of pesticide mixtures and limited residue data for bee-related products beyond honey raise concern. Future research should prioritize harmonized residue limits for all beekeeping matrices, standardized quality control and reporting practices, targeted mixture-toxicity assessment under realistic co-exposure scenarios, and the broader adoption of green, miniaturized, and matrix-tailored sample preparation strategies to enhance sensitivity, sustainability, and comparability across studies.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00300"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147385295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends of pretreatment and analysis methods for haloacetonitriles in environment","authors":"Ai Ren ,&nbsp;Yuan Zhang ,&nbsp;Yu Bian ,&nbsp;Hui-zhe Wu ,&nbsp;Xue-song Feng","doi":"10.1016/j.teac.2025.e00293","DOIUrl":"10.1016/j.teac.2025.e00293","url":null,"abstract":"<div><div>Haloacetonitriles (HANs) are toxic disinfection by-products frequently detected in treated water, posing risks to human health and the environment. Accurate and sensitive characterization of HANs remains challenging due to the volatility and trace-level concentrations. This review provides a comprehensive summary of recent advances in HANs pretreatment and detection over the past decade. While traditional techniques, such as liquid–liquid extraction and gas chromatography-based methods, remain widely used, novel approaches have emerged, including 3D-printed liquid phase microextraction devices, online extraction systems, and high-resolution mass spectrometry (e.g., Q-Exactive MS, Time-of-Flight MS). The application of self-fabricated materials, such as PDMS/DVB-NVP fibers in SPME, has further improved extraction performance. This review critically compares current strategies, highlights key limitations, and discusses future directions for developing efficient, automated, and environmentally friendly methods for HANs analysis.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00293"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145738031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical trends in the determination of alkylated polycyclic aromatic hydrocarbons in environmental samples: Challenges and advances","authors":"Gilda Albino Bento Jorge ,&nbsp;Beatriz dos Reis Lago Brandão ,&nbsp;Maria Elisabete Machado","doi":"10.1016/j.teac.2025.e00292","DOIUrl":"10.1016/j.teac.2025.e00292","url":null,"abstract":"<div><div>Alkylated polycyclic aromatic hydrocarbons (APAHs) are naturally present in petroleum and derivatives and are released into the environment through industrial processes and incomplete combustion of fossil fuels. Due to their hydrophobicity, stability and low polarity, these substances tend to accumulate across different environmental compartments. In the atmosphere, APAHs are detected in both particulate and gas phases, especially in urban and industrial regions. In aquatic systems, less hydrophobic APAHs, such as alkylated naphthalenes and phenanthrenes, are commonly found in surface waters, while sediments serve as long-term sinks for these compounds. In biota, APAHs exhibit bioaccumulation potential, with elevated levels reported in aquatic organisms, particularly in lipid-rich tissues. Despite their environmental relevance, APAHs remain understudied, primarily due to analytical challenges related to their structural diversity, low environmental concentrations, and co-elution of isomers. This review aims to explore the analytical approaches employed for the extraction, detection, identification, and quantification of APAHs in environmental matrices. Extraction and pre-concentration techniques, such as solid-phase extraction and microextraction, as well as the application of high-resolution chromatographic and mass spectrometric methods (e.g., GC×GC-TOFMS, GC×GC-HRMS, LC-MS/MS) for APAHs were emphasized. Individual APAHs and specific diagnostic ratios employed to indicate petrogenic and pyrogenic sources are presented. To the best of our knowledge, this is the first review focused on APAHs in environmental samples that comprehensively summarizes sample preparation procedures, instrumental analytical techniques, coelutions challenges and strategies for the separation and quantification of isomers.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00292"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide screening, design, and application for detecting small-molecule contaminants in food","authors":"Yunling Shao ,&nbsp;Miao Wang ,&nbsp;Yongxin She ,&nbsp;Zhen Cao ,&nbsp;Fen Jin ,&nbsp;Maojun Jin ,&nbsp;Jing Wang ,&nbsp;A.M. Abd El-Aty","doi":"10.1016/j.teac.2025.e00290","DOIUrl":"10.1016/j.teac.2025.e00290","url":null,"abstract":"<div><div>Small-molecule contaminants (SMCs), including pesticides, veterinary drugs, mycotoxins, and heavy metals, threaten food safety and human health. Traditional detection methods such as chromatography and immunoassays are accurate but costly, time-consuming, and less practical for rapid screening. Peptides have emerged as promising alternatives because of their small size, modifiability, high specificity, and compatibility with sensor platforms. This review integrates developments in traditional peptide screening, computational design, and analytical applications for detecting SMCs in food. It critically evaluates the strengths and limitations of current methods while exploring how artificial intelligence and nanomaterial-based strategies enhance peptide performance. By bridging peptide engineering and analytical chemistry, this review provides practical insights for developing rapid, cost-effective, and sensitive detection tools. It also identifies knowledge gaps and future opportunities, guiding both experienced researchers and newcomers to advance peptide-based assays toward real-world applications in food safety and environmental monitoring.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00290"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145618425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent sensors for trace detection of hydrocarbons, explosives, and drug residues in soil: Advances and critical insights","authors":"Hasher Irshad,&nbsp;Khadija,&nbsp;Katrine Qvortrup","doi":"10.1016/j.teac.2025.e00294","DOIUrl":"10.1016/j.teac.2025.e00294","url":null,"abstract":"<div><h3><strong>Background</strong></h3><div>Soil contamination by hydrocarbons, explosives, and pharmaceutical drugs, including antibiotics, poses serious environmental and health risks. These pollutants originate from industrial waste, military activities, and agriculture, leading to long-term toxicity. Sensitive detection methods are crucial for effective monitoring and remediation.</div><div><strong>Scope and Approach:</strong> This review covers recent advances in fluorescent sensors for detecting hydrocarbons, explosives, and pharmaceutical drug contaminants in soil. It highlights sensor materials, detection mechanisms, and array-based approaches. Additionally, the review addresses the challenges and limitations faced by these sensors when applied to real sample analysis.</div><div><strong>Key Findings and Conclusions:</strong> Fluorescent sensors offer high sensitivity, selectivity, and rapid detection of hydrocarbons, explosives, and drug residues. Advances in array based sensing and portable devices have improved detection efficiency. However, challenges remain in enhancing sensor durability and field applicability. Future research should focus on real-time monitoring and practical deployment for effective environmental assessment, as well as the development of reusable sensors, long-range pH stability, and the fabrication of devices such as Fido X4.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00294"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ubiquity of phthalates: Classification, environmental behavior, implications, and recent advances in analytical detection","authors":"Nurul Izzah Zainol ,&nbsp;Nurhamizah Rahmat ,&nbsp;Ahmad Farabi Mohamad Saman ,&nbsp;Mardiana Saaid ,&nbsp;M. Hazwan Hussin ,&nbsp;Siti Fatimah Nur Abdul Aziz","doi":"10.1016/j.teac.2026.e00296","DOIUrl":"10.1016/j.teac.2026.e00296","url":null,"abstract":"<div><div>Phthalates, extensively used as plasticizers in industrial and consumer products, have long been recognized for their toxicological impacts and environmental persistence. Their classification as endocrine-disrupting chemicals linked to reproductive dysfunction, metabolic disorders, and oxidative stress has amplified global regulatory concern. Compounding these risks is their widespread occurrence in aquatic ecosystems, where phthalates bioaccumulate and contribute to long-term ecological degradation. This review critically examines the classification, health implications, and environmental behavior of phthalates, while evaluating current detection technologies. Conventional chromatographic methods gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC) offer high sensitivity but are constrained by cost and operational complexity. Emerging spectrophotometric and electrochemical platforms offer cost-effective and portable alternatives but still face significant challenges such as matrix interference, limited selectivity, and poor reproducibility. Ultimately, bridging these analytical advancements with scalable, real-world applications is essential to effectively mitigate phthalate exposure risks to both human health and the environment.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00296"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From soils to edible tissues: Critical assessment of techniques for detecting micro- and nanoplastics in agroecosystems","authors":"Jesús Marín-Sáez ,&nbsp;Encarnación López-Rodríguez ,&nbsp;Antonia Garrido Frenich ,&nbsp;Roberto Romero-González","doi":"10.1016/j.teac.2026.e00297","DOIUrl":"10.1016/j.teac.2026.e00297","url":null,"abstract":"<div><div>The rapid accumulation of micro- and nanoplastics (MNPs) in agricultural landscapes has raised urgent questions about their fate in soils, their transfer to plants, and their eventual entry into human diets. While early reviews of MNPs in food matrices, soils, and plants provided important foundations, most treated these compartments separately and rarely aligned analytical evidence across the soil-plant-edible tissue continuum. This review synthesizes current knowledge along the agricultural soil-plant-food pathway, with emphasis on analytical workflows such as sampling, pretreatment, spectroscopic imaging, and thermoanalytical and mass-spectrometric quantification. We critically evaluate detection limits, recovery biases, and the suitability of these methods for complex agricultural matrices, while also highlighting emerging computational tools for particle recognition, automated spectral deconvolution, and improved mass-balance closure. Persistent challenges involving sub-50 µm fractions, nanoscale particles, and environmentally realistic concentrations are discussed. While earlier essential studies are included for context, the focus is on recent literature that captures the rapid evolution of analytical capabilities from 2020 to 2025. Linking field surveys, controlled uptake studies, and exposure modelling shows how harmonized protocols and improved QA/QC can strengthen dietary risk assessment. The review concludes by identifying methodological gaps and introducing recent advances in analytical determination, including advanced imaging techniques such as confocal laser scanning microscopy (CLMS) or photo-induced force microscopy (PiFM), thermoanalytical MS (e.g., pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS), thermal extraction and desorption (TED)-GC/MS and fast-MS platforms), and imaging-enhanced Raman/Fourier transform infrared spectroscopy (FTIR), together with novel data analysis workflows that now warrant an integrated appraisal focused on agroecosystems.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00297"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in TiO₂-based photocatalysts for environmental remediation: Structural engineering, mechanistic insights, and degradation pathways of organic and inorganic pollutants","authors":"Karma M. Albalawi ,&nbsp;Mohamed E. Eissa ,&nbsp;Khalil ur Rehman ,&nbsp;Mohammed Alissa ,&nbsp;Suad A. Alghamdi ,&nbsp;Mohammed A. Alshehri ,&nbsp;Ghfren S. Aloraini ,&nbsp;Alaa S. Alhegaili ,&nbsp;Mohamed Abdel-Megid ,&nbsp;Ehab A. Abdelrahman","doi":"10.1016/j.teac.2025.e00291","DOIUrl":"10.1016/j.teac.2025.e00291","url":null,"abstract":"<div><div>TiO₂-based photocatalysis has become a promising strategy for the degradation of volatile organic compounds (VOCs) and inorganic gaseous pollutants. This review provides an analytical examination of how structural design, surface engineering, and electronic modulation influence the photocatalytic performance of TiO₂ materials. Emphasis is placed on the role of crystal facets, oxygen vacancies, heterojunction interfaces, and metal/non-metal dopants in regulating charge-transfer dynamics and suppressing electron–hole recombination. Mechanistic pathways for the oxidation of aldehydes, aromatics, trichloroethene, NOx, SOx, CO, ozone, and H₂S are systematically outlined, focusing on radical formation and kinetic behavior. Finally, the review highlights key design principles and performance-governing factors that can guide the development of high-efficiency TiO₂ photocatalysts for future environmental remediation systems.</div></div>","PeriodicalId":56032,"journal":{"name":"Trends in Environmental Analytical Chemistry","volume":"49 ","pages":"Article e00291"},"PeriodicalIF":13.4,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145924892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}