Journal of Laboratory Medicine最新文献_第7页

Study on pathogen spectrum of 1,046 hospitalized children with respiratory tract infections during COVID-19 新冠肺炎期间1046例呼吸道感染住院患儿病原谱分析

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-01-12 DOI: 10.1515/labmed-2022-0104

Xinhong Han, Xuelie Wang, Jin Zhang, Xue-Lei Gong, Lilly Kan, Jieling Wei, Xiugong Zhang

{"title":"Study on pathogen spectrum of 1,046 hospitalized children with respiratory tract infections during COVID-19","authors":"Xinhong Han, Xuelie Wang, Jin Zhang, Xue-Lei Gong, Lilly Kan, Jieling Wei, Xiugong Zhang","doi":"10.1515/labmed-2022-0104","DOIUrl":"https://doi.org/10.1515/labmed-2022-0104","url":null,"abstract":"Abstract Objectives This study aimed to describe the pathogen spectrum of bacteria and viruses of RTIs in hospitalized children during the Coronavirus disease 2019 (COVID-19) epidemic in Shenzhen. Methods From October 2020 to October 2021, the results of pathogenic tests causing RTIs were retrospectively analyzed in hospitalized children in Shenzhen Luohu Hospital Group. Results 829 sputum samples for bacterial isolation and 1,037 nasopharyngeal swabs for virus detection in total. The positive detection rate (PDR) of bacteria was 42.1%. Staphylococcus aureus (18.8%) was the predominant bacteria detected in positive cases, with Moraxella catarrhalis (10.9%), Streptococcus pneumoniae (9.5%) following. The PDR of the virus was 65.6%. The viruses ranking first to third were Human Rhinovirus (HRV), Respiratory syncytial virus (RSV), and Human Parainfluenza (HPIV), with rates of 28.0, 18.1, and 13.5%, respectively. Children under 3 years were the most susceptible population to RTIs. The pathogens of S. aureus, M. catarrhalis, S. pneumoniae, HRV, and HPIV were more prevalent in autumn. Meanwhile, RSV had a high rate of infection in summer and autumn. S. aureus and HRV had higher co-infection rates. Conclusions Our findings demonstrate the pathogen spectrum of 1,046 hospitalized children with RTIs in Shenzhen, China, during the COVID-19 outbreak.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"47 - 54"},"PeriodicalIF":1.2,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46745390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibody titer 6 months after the third dose of COVID-19 mRNA vaccination 第三次接种COVID-19 mRNA后6个月的抗体滴度

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-12-07 DOI: 10.1515/labmed-2022-0092

Rikei Kozakai, Susumu Suzuki, Kuniko Hoshi, Yoshihiko Izumi, Shin-ichiro Takahashi

{"title":"Antibody titer 6 months after the third dose of COVID-19 mRNA vaccination","authors":"Rikei Kozakai, Susumu Suzuki, Kuniko Hoshi, Yoshihiko Izumi, Shin-ichiro Takahashi","doi":"10.1515/labmed-2022-0092","DOIUrl":"https://doi.org/10.1515/labmed-2022-0092","url":null,"abstract":"Abstract Objectives Administration of the third dose of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccine was initiated on December 1, 2021, in Japan. However, data on the long-term effects of this third vaccination remain scarce. Here, we examined the levels of SARS-CoV-2 antibodies in those who received the Pfizer BioNTech (BNT162b2) vaccine, 6 months after the third vaccination. Methods Samples from 40 healthy volunteers were used to measure SARS-CoV-2 antibodies with chemiluminescent assays against the receptor-binding domain (RBD) of the virus. Results At 445 days after the first dose of BNT162b2, which is 180 days after the third vaccination, the mean anti-RBD IgG level was 159.4 AU/mL (SD 100.1 AU/mL), which was significantly higher than 144 days after the second vaccination, while mean anti-RBD IgM was baseline level (0.4 C.O.I.). The decline in IgG, 180 days after the third vaccination, was 74.1% (SD 16.1%), which was significantly lower than the 88.6% (SD 4.4%) decline observed 144 days after the second vaccination. Furthermore, we revealed that the reduction in IgG from 14 to 180 days after the third vaccination showed a significant inverse correlation with age, and the higher antibody response in younger participants at 14 days after the third vaccination disappeared at longer time points. Conclusions The long-term durability of the IgG titer was significantly higher following the third vaccination compared with the second vaccination, and the reduction in IgG titer after the third vaccination inversely correlated with age.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"41 - 45"},"PeriodicalIF":1.2,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46201922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation laboratory prenatal screening performance based on biological variation of risk assessment in second trimester in China 基于妊娠中期风险评估生物学变异的实验室产前筛查效果评价

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-12-01 DOI: 10.1515/labmed-2021-0138

F. He, K. Zhong, S. Yuan, Yuxuan Du, Wei Wang, Zhiguo Wang

{"title":"Evaluation laboratory prenatal screening performance based on biological variation of risk assessment in second trimester in China","authors":"F. He, K. Zhong, S. Yuan, Yuxuan Du, Wei Wang, Zhiguo Wang","doi":"10.1515/labmed-2021-0138","DOIUrl":"https://doi.org/10.1515/labmed-2021-0138","url":null,"abstract":"Abstract Objectives This study intends to evaluate prenatal screening risk assessment performance based on biological variation to help clinical laboratories realize their own screening performance and set the appropriate performance specifications for prenatal screening risk assessment in China. Methods Fifteen samples with detailed clinical information were distributed to participants and the prenatal screening Down syndrome risk assessment of each sample were submitted. Three levels of performance specification (optimum, desirable, minimum) derived from biological variation were used to evaluate laboratory prenatal screening risk assessment performance. Results A total of 797 laboratories participated in the survey project. There are 216 laboratories using serological double-marker test and 581 laboratories using serological triple-marker test. For each screening protocol, more than 92.00% laboratories meet minimum performance specifications, more than 84.00% laboratories meet desirable performance specifications, and only about 62.50%laboratories meet optimum performance specifications. The Feltz and Miller test indicated that there were no significant statistical differences in the RCV for double-marker screening in 5 platforms and triple-marker screening in 6 platforms. Conclusions The risk assessment of prenatal screening in the second trimester in China can be improved further. It is appropriate to choose desirable performance specifications for external quality assurance organizations to evaluate the risk assessment performance.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"46 1","pages":"405 - 410"},"PeriodicalIF":1.2,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43616941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consolidation of quality control material improves patient safety and supports sustainability 质量控制材料的巩固提高了患者的安全性并支持可持续性

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-11-18 DOI: 10.1515/labmed-2022-0085

M. Menacho-Román, Salma Ouriach, Ignacio Arribas

引用次数: 2

Analytical performance of four rapid molecular testing for SARS-CoV-2 SARS-CoV-2四种快速分子检测的分析性能

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-11-17 DOI: 10.1515/labmed-2022-0073

Y. Katayama, Ryosei Murai, Yuki Sato, M. Moriai, Shinya Nirasawa, Masachika Saeki, Yuki Yakuwa, Y. Fujiya, K. Kuronuma, Satoshi Takahashi

{"title":"Analytical performance of four rapid molecular testing for SARS-CoV-2","authors":"Y. Katayama, Ryosei Murai, Yuki Sato, M. Moriai, Shinya Nirasawa, Masachika Saeki, Yuki Yakuwa, Y. Fujiya, K. Kuronuma, Satoshi Takahashi","doi":"10.1515/labmed-2022-0073","DOIUrl":"https://doi.org/10.1515/labmed-2022-0073","url":null,"abstract":"Abstract Objectives Various reagents and equipment for testing SARS-CoV-2 infections have been developed, particularly rapid molecular tests based on polymerase chain reaction (PCR). Methods We evaluated the analytical performance of four rapid molecular tests for SARS-CoV-2. We used 56 nasopharyngeal swabs from patients with confirmed SARS-CoV-2 infection; 36 diagnosed as positive by the Ampdirect™ 2019-nCoV Detection Kit (Shimadzu assay) were considered as true-positive samples. Results The sensitivity of Cobas® Liat SARS-CoV-2 and Flu A/B (Cobas) was the highest among the four molecular test kits. The limit of detection was 1.49 × 10−2 copies/µL (95% confidence interval [CI]: 1.46×10−2−1.51 × 10−2 copies/µL) for Cobas; 1.43 × 10−1 copies/µL (95% CI: 8.01×10−3−2.78 × 10−1 copies/µL) for Xpert® Xpress SARS-CoV-2 test (Xpert); 2.00 × 10−1 copies/µL (95% CI: 1.95×10−1-2.05 × 10−1 copies/µL) for FilmArray Respiratory Panel v2.1 (FilmArray); and 3.33 × 10 copies/µL (95% CI: 1.93 × 10–4.72×10 copies/µL) for Smart Gene® SARS-CoV-2 (Smart gene). Cobas also had a high sensitivity (100%) compared with Shimadzu assay. The sensitivities of Xpert, FilmArray, and Smart Gene were 97.2%, 97.2%, and 75.0%, respectively. The specificity of all tests was 100%. Conclusions In conclusion, the four rapid SARS-CoV-2 molecular test kits have high specificity and sensitivity for detecting SARS-CoV-2. As they are easy to use, they could be a useful method for detecting SARS-CoV-2.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"46 1","pages":"411 - 415"},"PeriodicalIF":1.2,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46379346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Results of using Luer-Lok access device for clinics, intensive care units, and emergency services with high pre-analytical errors: analysis of 491.850 samples 分析前误差较高的诊所、重症监护病房和急诊使用Luer-Lok接入装置的结果:分析491.850份样本

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-11-15 DOI: 10.1515/labmed-2021-0176

E. Menekşe, M. E. Duz, B. Avci, Alpaslan Ozturk, Mustafa Durmaz

{"title":"Results of using Luer-Lok access device for clinics, intensive care units, and emergency services with high pre-analytical errors: analysis of 491.850 samples","authors":"E. Menekşe, M. E. Duz, B. Avci, Alpaslan Ozturk, Mustafa Durmaz","doi":"10.1515/labmed-2021-0176","DOIUrl":"https://doi.org/10.1515/labmed-2021-0176","url":null,"abstract":"Abstract Objectives The effectiveness of the Luer-Lok™ Access Device (LL) with the intravenous catheter (IVC) on sample rejections, which is used to prevent primarily hemolysis in the emergency department (ED), clinics, and intensive care units (ICU), was examined. Methods A total of 491.850 samples of eight months from Amasya University Sabuncuoğlu Şerefeddin Training and Research Hospital were investigated retrospectively. Inpatient, intensive care unit and emergency department samples were included in the study. Pre- (BLL) and post-Luer Lok (ALL) rejection of the samples analyzed. In the BLL period, 3,463 rejection samples out of 253,818 (1.36%) in the September-December period of 2020; in the ALL period, 1819 rejected samples from 238,032 (0.76%) in January-April 2021 were analyzed for all reasons. Results It was determined that the use of LL significantly reduced all-cause sample rejections. In addition, a significant decrease was observed in the rates of hemolysis and clot-related rejection thanks to LL. Conclusions According to our study data, in patients with IVC with the LL device, the pressure brought by the syringe is mainly avoided, and reliable blood collection is achieved, similar to the routine vacutainer blood collection apparatus, and hemolysis- and clot-related sample rejections are largely prevented.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"46 1","pages":"399 - 403"},"PeriodicalIF":1.2,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42084913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qualification and quantification of plasma cell-free DNA after long-term storage conditions in patients with benign prostatic hyperplasia (BPH): a pilot study 良性前列腺增生症（BPH）患者长期储存后血浆无细胞DNA的鉴定和定量：一项初步研究

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-11-14 DOI: 10.1515/labmed-2022-0044

A. Bozkurt, Mehmet Gürbüzel, İ. Sayar, Soner Baydeniz, Y. Arslan

{"title":"Qualification and quantification of plasma cell-free DNA after long-term storage conditions in patients with benign prostatic hyperplasia (BPH): a pilot study","authors":"A. Bozkurt, Mehmet Gürbüzel, İ. Sayar, Soner Baydeniz, Y. Arslan","doi":"10.1515/labmed-2022-0044","DOIUrl":"https://doi.org/10.1515/labmed-2022-0044","url":null,"abstract":"Abstract Objectives Free DNA is used as a cancer biomarker due to its low cost, high applicability, and fast, reliable results compared to invasive methods. This study aimed to evaluate the quantification of plasma-free DNA after long-term storage conditions and perform qualification through single nucleotide polymorphism (SNP) screening based on this DNA. Methods Plasma-free DNA samples were quickly isolated from the peripheral blood of both the benign prostatic hyperplasia (BPH) and control group participants and then maintained at −80 °C for four years. Upon thawing, first, free DNA was purified and fluorometric measurements were taken to determine the amount of DNA. Subsequently, the rs6983267, rs12628, and rs1799939 SNPs were screened in the CCAT2, HRAS, and RET genes, respectively. Results Significant results were obtained from the fluorometric measurements in terms of single-stranded DNA (ssDNA) (p<0.001). However, there was no significant difference in SNPs rs6983267, rs12628, and rs1799939 in the BPH group compared to the healthy individuals. Conclusions The data show that fluorometric ssDNA measurements are suitable for quantifying free DNA. The fact that SNP screening can be done successfully in both healthy people and BPH patients suggests that plasma-free DNA can be stored in the laboratory under appropriate conditions.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"46 1","pages":"383 - 389"},"PeriodicalIF":1.2,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44495498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of cigarette smoking on the serum levels of calcium and 25 hydroxy vitamin D in patients with postmenopausal osteoporosis 吸烟对绝经后骨质疏松症患者血清钙和25-羟基维生素D水平的影响

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-11-03 DOI: 10.1515/labmed-2022-0061

Lifang Wu, Q. Du

{"title":"The effect of cigarette smoking on the serum levels of calcium and 25 hydroxy vitamin D in patients with postmenopausal osteoporosis","authors":"Lifang Wu, Q. Du","doi":"10.1515/labmed-2022-0061","DOIUrl":"https://doi.org/10.1515/labmed-2022-0061","url":null,"abstract":"Abstract Objectives Deficiency of calcium and vitamin D is a common finding in postmenopausal osteoporosis (PMOP). However, the effect of cigarette smoking on the serum levels of calcium and 25-hydroxyvitamin D [25(OH)D] remains inconclusive. Methods The data of 432 inpatients with PMOP between January 2016 and October 2019 were collected from the hospital information system of the Second Hospital of Shanxi Medical University. The associations between smoking habits and serum calcium and 25(OH)D levels were analyzed by multiple linear regression. The intensity and duration of smoking were also conducted in the analysis to detect the dose-dependent effect of cigarette smoking. Results Significant differences were found between smokers and never smokers regarding serum calcium and 25(OH)D levels. The multiple linear regression model showed significant negative associations of the daily number of cigarette smoking and the smoking durations with the serum calcium and 25(OH)D levels. Moreover, the effect of smoking on the decreased levels of serum calcium and 25(OH)D showed a dose-dependent manner. Conclusions Cigarette smoking was negatively associated with the serum calcium and 25(OH)D levels in patients with PMOP. Early detection of serum calcium and 25(OH)D may contribute to predicting fracture risk, and smoking cessation intervention is necessary for patients with POMP.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"71 2","pages":"391 - 397"},"PeriodicalIF":1.2,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41280137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-invasive prenatal paternity testing using mini-STR-based next-generation sequencing: a pilot study 使用基于迷你STR的下一代测序的无创产前亲子鉴定：一项试点研究

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-09-21 DOI: 10.1515/labmed-2021-0191

Wenqian Song, Nan Xiao, Shihang Zhou, Weijian Yu, Ni Wang, L. Shao, X. Liang

{"title":"Non-invasive prenatal paternity testing using mini-STR-based next-generation sequencing: a pilot study","authors":"Wenqian Song, Nan Xiao, Shihang Zhou, Weijian Yu, Ni Wang, L. Shao, X. Liang","doi":"10.1515/labmed-2021-0191","DOIUrl":"https://doi.org/10.1515/labmed-2021-0191","url":null,"abstract":"Abstract Objectives To assess the efficacy of a mini-STR-based next-generation sequencing (NGS) method for non-invasive prenatal paternity testing (NIPPT). Methods Plasma DNA from 28 pregnant women was extracted and cell-free foetal DNA (cffDNA) genotyping was performed at 23 mini-STR loci using the Illumina NextSeq 500 system. For each mini-STR locus, the cffDNA genotype was validated by determining infant DNA genotype. The mini-STR loci with high concordance rates were selected for the comparison of STR genotyping results between cffDNA and biological father DNA or random male DNA for each family. Results The biological relationship was identified between alleged fathers and infants in all 28 families using the capillary electrophoresis (CE) method. Moreover, the concordance rates of STR genotypes D5S818, D19S253, and D21S1270 were less than 50% in 23 autosomal STR loci. The STR genotype matching probability was calculated using 20 STR loci with more than 60% concordance rate. There was a significant difference in the STR genotype matching probability between cffDNA and the DNA from the biological father (75–100%) or from random males (25–70%) (p<0.0001). Conclusions Our study demonstrated that mini-STR can be used for NGS-based NIPPT. Furthermore, this method can be used for crime control purposes using the STR data available from the national forensic DNA databases.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"46 1","pages":"337 - 344"},"PeriodicalIF":1.2,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45528096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of short post-apnea time on plasma triglycerides, lipoprotein and cholesterol derived oxysterols levels 呼吸暂停后短时间对血浆甘油三酯、脂蛋白和胆固醇衍生的氧化甾醇水平的影响

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2022-09-12 DOI: 10.1515/labmed-2022-0042

R. Dolscheid-Pommerich, B. Stoffel‐Wagner, Madlen Reinicke, F. Stellaard, D. Lütjohann, L. Eichhorn

{"title":"The effect of short post-apnea time on plasma triglycerides, lipoprotein and cholesterol derived oxysterols levels","authors":"R. Dolscheid-Pommerich, B. Stoffel‐Wagner, Madlen Reinicke, F. Stellaard, D. Lütjohann, L. Eichhorn","doi":"10.1515/labmed-2022-0042","DOIUrl":"https://doi.org/10.1515/labmed-2022-0042","url":null,"abstract":"Abstract Objectives Apnea diving is characterized by extreme hypoxia and hypercapnia. Possible pathophysiological processes concerning the cardiovascular system are not yet fully understood. Hypoxia has effects on triglyceride metabolism and circulating blood lipids. To date, in voluntary apnea divers, no short-time hypoxia expositions focusing on plasma triglycerides, lipoprotein and cholesterol derived oxysterols levels have been performed. We hypothesize that short time hypoxemia leads to altered triglyceride, cholesterol, and oxysterol plasma levels in voluntary apnea divers. Methods Ten athletes performed apnea under dry conditions in a horizontal position. Plasma levels of lipids, lipoproteins and oxysterols were determined with turbidimetric immunoassays, gas chromatography (GC) - flame ionization detection (FID) and GC-MS-SIM before apnea, immediately after apnea and 0.5 h after apnea. All sterols and oxysterols were corrected for GC-FID cholesterol as measured in the same sample. Spearman’s rank correlation test was performed and pairwise comparison of absolute and cholesterol corrected plasma levels from the different sampling dates was conducted using a robust mixed linear model. Results We observed significantly reduced levels of apolipoprotein B, triglycerides, cholesterol, high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol, and oxysterols (7β-OHC, 24-OHC, 27-OHC and 7-KC) for different time points. Cholesterol corrected plasma levels of the oxysterols showed no significant changes after short post-apnea time except for a significant elevation of the cholestane-3β, 5α, 6β-triol ratio. Conclusions We could observe that a single short time hypoxemia under dry conditions in voluntary apnea divers leads to altered triglyceride, cholesterol and oxysterol plasma levels.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"46 1","pages":"345 - 351"},"PeriodicalIF":1.2,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48158324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0