Journal of Laboratory Medicine最新文献_第6页

Keep cool! Observed temperature variations at different process stages of the biobanking workflow – examples from the Leipzig medical biobank 保持冷静!观察到的温度变化在生物银行工作流程的不同过程阶段-来自莱比锡医学生物银行的例子

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-03-24 DOI: 10.1515/labmed-2022-0167

J. Weikert, Angelina Mehrländer, R. Baber

{"title":"Keep cool! Observed temperature variations at different process stages of the biobanking workflow – examples from the Leipzig medical biobank","authors":"J. Weikert, Angelina Mehrländer, R. Baber","doi":"10.1515/labmed-2022-0167","DOIUrl":"https://doi.org/10.1515/labmed-2022-0167","url":null,"abstract":"Abstract Objectives Biobanked samples are becoming increasingly important for research outcomes. Most of the biobanking processes (from preparation to storage) are affected by temperature in a time-dependent manner and have a high impact on sample quality. We aimed to validate time and temperature sensitive processes such as sample preparation, transport, sorting, and storage, which have a high impact on sample quality. Methods Temperature was measured using internal or external temperature data loggers. We analysed the temperature and present real data from our sample transport on dry ice and with the CryoPod, from our ultra-low temperature freezers (UTFs) of different manufacturers and cryostores. We also tested sample sorting on dry ice and in a cryogenic workbench. Results In the UTFs, we identified temperature zones with a temperature difference from 4.7 °C up to 20.8 °C across the whole UTF. For sample transport within approximately 30 min we observed temperatures of −80.2 °C ± 4.0 °C and −173.9 °C ± 16.9 °C for dry ice boxes and the CryoPod, respectively. Sorting on dry ice was best in a polystyrene box half-filled with dry ice pellets, although the temperature increased by 7.5 °C within the first 5 min, whereas the temperature in the cryogenic workbench remained stable below −100 °C for hours. Conclusions Time and temperature play a crucial role in the entire biobanking process, with sometimes immense temperature fluctuations in some equipment. We recommend the validation or verification of all equipment and processes used to avoid pre-analytical errors in accordance with DIN EN ISO 20387.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"69 - 80"},"PeriodicalIF":1.2,"publicationDate":"2023-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44933070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A case report of pseudoleukopenia: playing hide-and-seek 假白细胞减少症一例报告：玩捉迷藏游戏游戏

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-03-24 DOI: 10.1515/labmed-2022-0155

Sixuan Wang, Xiaoxi Wang, Ling-bin Shu

引用次数: 0

Congress report: 5th Munich POCT Symposium, September 27–29, 2022, Klinikum rechts der Isar der TU München 大会报告:第五届慕尼黑POCT研讨会，2022年9月27日至29日，Klinikum rets der Isar der TU m<s:1> nchen

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-03-22 DOI: 10.1515/labmed-2023-0025

Ralf Junker, P. Luppa

引用次数: 1

Age-and sex-specific reference intervals of total cholesterol, LDL cholesterol, HDL cholesterol and non-HDL cholesterol. Comparison of two algorithms for the indirect estimation of reference intervals 总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇和非高密度脂蛋白胆固醇的年龄和性别特异性参考区间。参考区间间接估计两种算法的比较

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-02-22 DOI: 10.1515/labmed-2022-0147

R. Haeckel, T. Ammer, W. Wosniok, Alexander Krebs, A. Torge, Mustafa Özçürümez, Alexander Bertram

{"title":"Age-and sex-specific reference intervals of total cholesterol, LDL cholesterol, HDL cholesterol and non-HDL cholesterol. Comparison of two algorithms for the indirect estimation of reference intervals","authors":"R. Haeckel, T. Ammer, W. Wosniok, Alexander Krebs, A. Torge, Mustafa Özçürümez, Alexander Bertram","doi":"10.1515/labmed-2022-0147","DOIUrl":"https://doi.org/10.1515/labmed-2022-0147","url":null,"abstract":"Abstract Objectives Reference intervals of total cholesterol concentrations in plasma and of their fractions low-density lipoprotein (LDL)-, high-density lipoprotein (HDL)-and non-HDL concentrations are seldom studied with respect to the relevance of age and sex. Therefore, the effect of age and sex on the reference intervals was reinvestigated with 2 indirect procedures. Methods As an indirect approach, the truncated minimum chi-square method was applied. All analyses were performed by computer programs available. The script published on the homepage of the German Society of Clinical Chemistry and Laboratory Medicine (DGKL) allows to derive a continuous age dependency of reference intervals together with their confidence and equivalence limits. The results of this approach were compared with those obtained by an indirect method developed more recently, the refineR algorithm. Results In the present study, the upper reference limits of total cholesterol varied from 5.1 to 7.8 mmol/L (197–302 mg/dL) depending on various biological variables (as age, sex, inpatients versus outpatients). These upper limits increased with age. Differences between sexes can be neglected except for the age above 80 years. The pattern of reference limits of LDL cholesterol and non-HDL cholesterol paralleled those of total cholesterol. The reference limits of HDL cholesterol were higher in women than in men but were independent of age. Conclusions Reference limits for the concentrations of total cholesterol and their fractions LDL-, HDL-and non-HDL concentrations should be stratified for age and sex.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"55 - 62"},"PeriodicalIF":1.2,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66984102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased hemolysis rate in plasma tubes after implementation of a fully automated sample delivery and acceptance system 在实施全自动样品输送和验收系统后，血浆管中的溶血率增加

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-02-06 DOI: 10.1515/labmed-2022-0154

Jonathan A. Saenger, J. Atamaniuk, M. Gaggl, Johannes Asenbaum, Florian A. Huber, A. Grieb, M. Födinger

{"title":"Increased hemolysis rate in plasma tubes after implementation of a fully automated sample delivery and acceptance system","authors":"Jonathan A. Saenger, J. Atamaniuk, M. Gaggl, Johannes Asenbaum, Florian A. Huber, A. Grieb, M. Födinger","doi":"10.1515/labmed-2022-0154","DOIUrl":"https://doi.org/10.1515/labmed-2022-0154","url":null,"abstract":"Abstract Objectives Automated sample delivery and laboratory acceptance systems (PTAS) may influence the hemolysis rate of blood samples due to g-forces, abrupt acceleration, and rapid deceleration. However, quantitative data regarding the rate of hemolysis in PTAS is limited. To fill this void, the effect of a pneumatic tube in combination with an acceptance system (PTAS) on the hemolysis rate was investigated in this study. Methods Lithium heparin plasma tubes were transported from different clinical departments to the hospital’s laboratory (a) by employees or (b) with an automated PTAS and analyzed for the presence of hemolysis based on a hemolysis index (HI) of >25. Hemolysis indices of 68.513 samples were retrieved from the laboratory information system before and after installation of the PTAS and were subjected to statistical analysis. Results A total of 32.614 samples were transported by employees, of which 3.815 samples (11.70%) were hemolytic, and 9.441 out of 35.899 samples delivered by PTAS (26.30%) were hemolytic. After the implementation of the PTAS, hemolysis rates increased in all departments. Conclusions Automated PTAS are associated with increased hemolysis rates. This has implications for routine patient management and should be considered for the transportation of samples used for the determination of hemolysis-sensitive laboratory parameters.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"63 - 68"},"PeriodicalIF":1.2,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46844271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new machine-learning-based prediction of survival in patients with end-stage liver disease 一种新的基于机器学习的终末期肝病患者生存预测

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-02-01 DOI: 10.1515/labmed-2022-0162

Sebastian Gibb, Thomas Berg, A. Herber, B. Isermann, T. Kaiser

{"title":"A new machine-learning-based prediction of survival in patients with end-stage liver disease","authors":"Sebastian Gibb, Thomas Berg, A. Herber, B. Isermann, T. Kaiser","doi":"10.1515/labmed-2022-0162","DOIUrl":"https://doi.org/10.1515/labmed-2022-0162","url":null,"abstract":"Abstract Objectives The shortage of grafts for liver transplantation requires risk stratification and adequate allocation rules. This study aims to improve the model of end-stage liver disease (MELD) score for 90-day mortality prediction with the help of different machine-learning algorithms. Methods We retrospectively analyzed the clinical and laboratory data of 654 patients who were recruited during the evaluation process for liver transplantation at University Hospital Leipzig. After comparing 13 different machine-learning algorithms in a nested cross-validation setting and selecting the best performing one, we built a new model to predict 90-day mortality in patients with end-stage liver disease. Results Penalized regression algorithms yielded the highest prediction performance in our machine-learning algorithm benchmark. In favor of a simpler model, we chose the least absolute shrinkage and selection operator (lasso) regression. Beside the classical MELD international normalized ratio (INR) and bilirubin, the lasso regression selected cystatin C over creatinine, as well as IL-6, total protein, and cholinesterase. The new model offers improved discrimination and calibration over MELD and MELD with sodium (MELD-Na), MELD 3.0, or the MELD-Plus7 risk score. Conclusions We provide a new machine-learning-based model of end-stage liver disease that incorporates synthesis and inflammatory markers and may improve the classical MELD score for 90-day survival prediction.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"13 - 21"},"PeriodicalIF":1.2,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41686192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The lncRNA prostate androgen-regulated transcript 1 (PART-1) promotes non-small cell lung cancer progression by regulating the miR-204-3p/IGFBP-2 pathway lncRNA前列腺雄激素调节转录物1（PART-1）通过调节miR-204-3p/IGFBP-2途径促进非小细胞肺癌癌症进展

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-02-01 DOI: 10.1515/labmed-2022-0082

Kenfen Li, Yanping Zhang, Yunfeng Wang, Xin Guo, Xianhui Dai, L. Song

{"title":"The lncRNA prostate androgen-regulated transcript 1 (PART-1) promotes non-small cell lung cancer progression by regulating the miR-204-3p/IGFBP-2 pathway","authors":"Kenfen Li, Yanping Zhang, Yunfeng Wang, Xin Guo, Xianhui Dai, L. Song","doi":"10.1515/labmed-2022-0082","DOIUrl":"https://doi.org/10.1515/labmed-2022-0082","url":null,"abstract":"Abstract Objectives Lung cancer is a common malignant tumour of the lung and the leading cause of cancer mortality worldwide. Non-small cell lung cancer (NSCLC) accounts for 80–85% of lung cancers, and 40% of NSCLCs have spread beyond the lungs by the time they are diagnosed. The long noncoding RNA (lncRNA) prostate androgen-regulated transcript 1 (PART-1) has been reported to promote the development of several cancers. Methods In the current study, we investigated the role of PART-1 in the proliferation, invasion, and migration of NSCLC. Results The expression levels of the PART-1 gene were higher in NSCLC cell lines, including A549, H1229, H1650, H1975, and PC9, than in human bronchial epithelia (HBE) cell lines. Knocking down PART-1 inhibited the proliferation, invasion, and migration of A549 cells and decreased tumour proliferation in nude mice. We confirmed that PART-1 targeted miR-204-3p directly and that miR-204-3p targeted insulin-like growth factor binding protein 2 (IGFBP-2) directly. Furthermore, we discovered that PART-1 impacts NSCLC progression by regulating the miR-204-3p-targeted IGFBP-2 pathway. Conclusions The lncRNA PART-1 might be a target for treating NSCLC and an early marker in the diagnosis of early lung cancer.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"31 - 40"},"PeriodicalIF":1.2,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46976919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating pyridoxal 5′-phosphate in serum and whole blood: implications for assessment of vitamin B6 status 血清和全血中循环的5′-磷酸吡哆醛：对评估维生素B6状态的意义

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-02-01 DOI: 10.1515/labmed-2022-0081

R. Obeid, Christoph Möller, J. Geisel

{"title":"Circulating pyridoxal 5′-phosphate in serum and whole blood: implications for assessment of vitamin B6 status","authors":"R. Obeid, Christoph Möller, J. Geisel","doi":"10.1515/labmed-2022-0081","DOIUrl":"https://doi.org/10.1515/labmed-2022-0081","url":null,"abstract":"Abstract Objectives Concentrations of pyridoxal 5′-phosphate (PLP) in serum and whole blood are routinely measured. The suitability of these markers in capturing vitamin B6 insufficiency is not well studied. Methods In 212 subjects, concentrations of PLP and thiamine pyrophosphate (TPP) were simultaneously measured in EDTA-whole blood using Chromsystems® (52052) method on HPLC devices. The whole blood PLP concentrations were compared to serum PLP concentrations measured using reagents from Immundiagnostik® (KC 2100). The whole blood TPP concentrations measured with the Chromsystems® (52052) were compared to those measured by the Chromsystems® (35000) method. Concentrations of homocysteine (tHcy) and cystathionine (Cys) were measured and used to judge the PLP methods. Results Serum PLP concentrations were on average 41% lower than whole blood PLP [mean (SD)=55.4 (83.0) vs. 131 (217) nmol/L]. Serum and whole blood PLP showed a strong correlation [Pearson correlation coefficient=0.724, p<0.001, n=204]. Eighty-five samples showed discrepant results for PLP status (serum PLP ≤30 nmol/L, but whole blood PLP >51 nmol/L) while 102 samples showed coherent results (reference group). The discrepancy group showed higher odds ratio for elevated tHcy >12.0 μmol/L compared to the reference group [OR (95% confidence intervals, CI)=2.1 (1.2–4.0)]. The OR (95% CI) of elevated Cys >300 nmol/L was 1.9 (1.0–3.5) in the discrepancy group compared to the reference group. TPP concentrations were 6% lower when using the Chromsystems®, 52052 compared to levels measured with Chromsystems®, 35000. Conclusions Serum and whole blood PLP concentrations disagree in a substantial number of samples. Serum PLP was better in reflecting elevated tHcy and Cys compared to whole blood PLP. Whole blood PLP underestimates the prevalence of vitamin B6 insufficiency. Methods of measuring TPP concentrations in whole blood were exchangeable.","PeriodicalId":55986,"journal":{"name":"Journal of Laboratory Medicine","volume":"47 1","pages":"23 - 29"},"PeriodicalIF":1.2,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45804075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frontmatter 头版头条

4区医学

Journal of Laboratory Medicine Pub Date : 2023-02-01 DOI: 10.1515/labmed-2023-frontmatter1

引用次数: 0

Oxidative stress and antioxidants in health and disease 健康和疾病中的氧化应激和抗氧化剂

IF 1.2 4区医学

Journal of Laboratory Medicine Pub Date : 2023-02-01 DOI: 10.1515/labmed-2022-0108

T. Kıran, O. Otlu, A. Karabulut

引用次数: 9