中华地方病学杂志最新文献

{"title":"Evaluation of academic influence of medical statistics source journals in Heilongjiang province during 2005 - 2009","authors":"Xinying Ma, Rong-hua Guo, Ying Li, Dan-na Wang, Yang Liu, Jing Zhou, Yiyi Zhang","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.05.033","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.05.033","url":null,"abstract":"Objective To evaluate the academic influence of medical statistics source journals in Heilongjiang province during 2005 - 2009. Methods Journal evaluation data of \"Chinese Science and Technology Journal Citation Reports\"(2006 - 2010 periodicals) as a statistical source, the 7 indexes(literature sources, total cites, impact factor, immediacy index, he cited rate, ratio of overseas papers, ratio of funded papers) of medical statistics source journals in Heilongjiang province during 2005 - 2009 were analyzed. Dynamic evaluation model was used to evaluate the academic influence of journals and compute impact assessment score(Ⅰ), relative impact assessment score(Ir), and rate of growth. Results There were 5 kinds of medical statistics source journals in Heilongjiang province during 2005 - 2009, they were \"Journal of Harbin Medical University\", \"Chinese Journal of Endemiology\", \"Chinese Journal of Critical Care Medicine\", \"Chinese Journal of Preventive Veterinary Medicine\",\"Chinese Journal of Traditional Medical Science and Technology\", respectively. Year by year, the total cites,impact factor and ratio of funded papers of above mentioned journals showed increase trend, literature sources, he cited rate and ratio of overseas papers showed stable trend, immediacy index showed greater fluctuations. Ⅰ values of above periodicals were 32 279.79, 379 379.20, 251 240.26, 117 576.00, and 83 156.65, respectively. Ir value of above periodicals were 0.19, 2.20, 1.45, 0.68, and 0.48, respectively. The rates of growth of above periodicals were 0.41, - 0.07, 0.24, 1.04, and 0.87, respectively. Conclusions The development of medical statistics source journals in Heilongjiang province is in good condition, the academic influence is an overall upward trend. \u0000 \u0000Key words: \u0000Journal;  Academic influence;  Outcome assessment","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"46 1","pages":"580-582"},"PeriodicalIF":0.0,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80600680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of surveillance results on human brucellosis in 2009 and 2010 in Gansu province","authors":"Wei-min Pan, Li-li Chen, Jin-xiao Xi, Hong Zhang, Ding-sheng Wang","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.05.022","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.05.022","url":null,"abstract":"Objective Through analyzing the epidemic characteristics and laws of human brucellosis in Gansu province during the past two years,to provide the basis for control of the disease. Methods Using \"China Information System for Diseases Control and Prevention V2.0\" and survey data of human brucellosis outbreak,we calculated the incidence of brucellosis and the composition of new cases and chronic cases in 2009 and 2010, respectively, and analyzed the three distributions of the disease. Results Brucellosis incidence was 0.37 per million in 2009, and 1.7 per million in 2010. The new cases accounted for 54.6％(83/152) and 51.8％(43/83),and chronic cases were 53.3％ (81/152) and 56.6％ (47/83), respectively. About 1/3 of the counties (cities,districts) was found to be with the disease, and most cases were clustered in Hexi Corridor and Longdong area of Gansu province. There were three counties with outbreak. Time distribution of the disease was jagged. May, July,September and December were high, with September the highest. Sex ratio was 3 : 1, and mean age was 46 years old.67.7％ (159/235) of cases were farmers, followed by retired officers and herders, with a percentage of about 7％,respectively. Conclusions Epidemic of human brucellosis shows a rapidly rising trend in Gansu province. There are new patients throughout the year, and young farmers are the main victims. A high proportion of chronic brucellosis is more harmful. Strengthen the prevention and control of the disease to increase their brucellosis protective awareness. \u0000 \u0000Key words: \u0000Brucellosis;  Data collection;  Outcome assessment","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"8 1","pages":"549-551"},"PeriodicalIF":0.0,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90872510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of monitoring results of Kaschin-Beck disease in Shandong province from 1996 to 2010","authors":"Zhong-jie Yun, Pei-zhong Chen, Yu-tao Wang, Jie Gao, Jicuang Hao, Heng-xiang Li, Enyu Pan, Wei-guo Li, Jie Liu","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.05.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.05.016","url":null,"abstract":"Objective To investigate the current status of Kaschin-Beck disease in Shandong province, and to provide a scientific basis for decision-making in controlling the disease. Methods According to the \"National Monitoring Program of Kaschin-Beck disease\" requirements, historical serious villages of Kaschin-Beck disease in Qingzhou of Shandong province were selected annually; children aged 7 to 16 were chosen to receive clinical examination and children aged 7 to 12 were taken X-ray examination. Clinical and X-ray diagnosis was carried out according to the \"Diagnostic Criteria of Kashin Beck Disease\"(GB 16003-1995). Results From 1996 to 2010, in 53 diseased villages, three thousand three hundred and eighteen school children aged 7 to 16 were clinically diagnosed, and child Kaschin-Beck disease of degree Ⅰ and above were not detected; three thousand and ninety-one school children aged 7 to 12 were examined by X-ray, forty cases were found positive, and the total positive rate was 1.29％(40/3091 ). The year with the highest positive rate was 2002, and the rate was 3.49％(13/372) ; the positive rate was 0 in 1996 and 2008. The difference of the X-ray positive rate between each year was statistically significant(x2 =31.54, P ＜ 0.01 ). Conclusions Child Kashin-Beck disease in Qingzhou is basically under control.Since etiology of Kashin-Beck disease is still unclear, surveillance of the disease still needs to be strengthened. \u0000 \u0000Key words: \u0000Kaschin-Beck disease;  Monitoring;  Data collection;  Outcome assessment","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"16 1","pages":"527-529"},"PeriodicalIF":0.0,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85995784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method with low usage amount of arsenic trioxide for measuring high concentration iodine in urine by As3+-Ce4+ catalytic spectrophotometry using ammonium persulfate digestion","authors":"Ya-ping Zhang, Yan-hong Huang, Yueyue Yan, Na Li","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.05.027","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.05.027","url":null,"abstract":"Objective To establish a new method with low usage amount of arsenic trioxide for measuring 300 - 1200 μg/L high concentration iodine in urine by As3+-Ce4+ catalytic spectrophotometry using ammonium persulfate digestion, which would be convenient for monitoring urinary iodine in excessive iodine regions and to reduce environmental arsenic pollution. Methods Calibrators and urine samples(0.20 ml each) were digested according to the current standard detection method of urinary iodine(WS/T 107-2006). At the same time, improving the current standard method, the amount of arsenious acid solution was reduced from 0.100 moL/L H3AsO3 (containing NaCl 25 g/L) 2.5 ml to 0.025 mol/L H3AsO3(containing NaCl 40 g/L) 2.5 ml; amount of ceric ammonium sulfate solution was reduced from 0.076 mol/L 0.30 ml to 0.025 mol/L 0.50 ml; and photometric wavelength was changed from 420 nm to 380 nm. The new method was evaluated by standard curve linearity and linear range, sample detection precision, accuracy, and the results of urinary iodine were compared with those determined bycurrent standard method, and this new method was also tested of suitable combination of reaction temperature and reaction time of cerium arsenic in the temperature range of 20 - 30 ℃. Results The calibration relation of C =a + blgA (C: iodine concentration, A : measuring absorhance) in the new method existed when As3+- Ce4+ catalytic reaction was kept at a certain stable temperature range between 20 - 30 ℃ and in certain fixed reacting time. The linear range of the calibration curve was 300 - 1200 μg/L and the linear correlative coefficient was- 0.9999. The relative standard deviations(RSD) were 1.0％(3.2/330.3), 0.4％(2.0/517.3), 0.5％(3.9/712.6) and 0.9％(9.4/1042.3) when measuring urine samples with iodine concentration of 330.3, 517.3,712.6, and 1042.3 μg/L, respectively. The total average recovery was 98.3％ with a range of 93.4％ (186.8/200.0) - 101.5％ (202.9/200.0) when measuring 4 urine samples containing different concentration of high iodine, and average recovery was 99.1％ (148.6/150.0), 97.5％ (195.0/200.0), 98.8％ (395.3/400.0), and 98.2％ (392.7/400.0),respectively. The test results of four urinary iodine standard materials were all within the given value range and the relative deviations(RD) were all ＜ 2.0％ at different test temperature, respectively. No significant difference was found between the results of the 16 urine samples containing high concentration of iodine determined by the new method and the current standard method (|t| =0.727, P ＞ 0.05). The table of suitable combination of As3+-Ce4+ reaction temperature and reaction time for this method was obtained(such as 20 ℃ and 33 min, 25 ℃ and 25 min,30 ℃ and 19 min, etc). Conclusions This method greatly reduces the amount of arsenic in waste, reduces pollution, saves reagents, and this method is easier to be performed with better precision and accuracy, which is suitable for measuring high concentration of iodine in urine. \u0000 \u0000Key words:","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"29 2 1","pages":"563-568"},"PeriodicalIF":0.0,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78791090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of tert-butylhydroquinone on sodium arsenite-induced cytotoxicity and oxidative injuries","authors":"L. Bing, L. Xin, B. Zhu, Xinyu Zhang, Xiaoyue Xing, Dan Liu, Xin Wang, Gui-fan Sun","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.05.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.05.005","url":null,"abstract":"Objective To study the protective effects of tert-butylhydroquinone(tBHQ) on sodium arsenite (NaAsO2)-induced cytotoxicity and oxidative injuries. Methods Chang liver cells were pretreated with tBHQ[0(control), 5, 25 μmol/L]for 24 h, and then co-treated with tBHQ(5 μmol/L) together with NaAsO2[0(control),30, 40, 50, 60 μmol/L] for another 24 h, and Alamar blue reduction rates were used to evaluate cell viability,the results were expressed as the relative ratio of Alamar blue reduction rates between the experimental group and the control group. On the other hand, Chang liver cells were pretreated with tBHQ[0(control), 5, 25 μmol/L] for24 h,and then co-treated with tBHQ(5 μmol/L) together with NaAsO2[0(control), 40, 50 μmol/L] for another 24 h,and the levels of cellular reactive oxygen species(ROS) were detected by staining cells with 2',7'-dichlorofluorescin diacetate(DCFH-DA), the results were expressed as the relative ratio of mean fluorescence intensity between the experimental group and the control group. Results Cell viability decreased dramatically by treatment with NaAsO2(30, 40, 50, 60 μmol/L), while relieved to some extent by pretreatment with 5, 25 μmol/L tBHQ, the main effects of NaAsO2 and tBHQ, as well as their interaction were all statistically significant(F =566.57, 55.09, 14.50,all P ＜ 0.05) ; the cell viability of NaAsO2(30, 40, 50, 60 μmol/L) pretreated with tBHQ(5, 25 mol/L) were 0.75 ±0.02, 0.70 ± 0.04, 0.59 ± 0.03, 0.43 ± 0.03 and 0.75 ± 0.02, 0.73 ± 0.03, 0.65 ± 0.02, 0.50 ± 0.02, respectively,all significantly higher than corresponding NaAsO2 alone groups(0.70 ± 0.03, 0.64 ± 0.03, 0.43 ± 0.03, 0.33 ±0.01, all P ＜ 0.05), the cell viability of NaAsO2(50, 60 μmol/L) pretreated with 25 μmol/L tBHQ was higher than corresponding 5 μmol/L tBHQ pretreatment groups(all P ＜ 0.05). On the other hand, 40, 50 μmol/L of NaAsO2 significantly induced hepatocellular ROS generation, while tBHQ(5, 25 μ mol/L) pretreatment significantly decreased NaAsO2-induced intracellular ROS levels, the main effects of NaAsO2 and tBHQ, as well as their interaction were all statistically significant (F =181.78, 60.55, 4.93, all P ＜ 0.05) ; the ROS levels of NaAsO2(40, 50 μ mol/L) pretreated with tBHQ(5, 25 μmol/L) were 1.87 ± 0.09, 1.80 ± 0.07 and 1.36 ± 0.11, 1.44 ± 0.12,all significantly decreased than corresponding NaAsO2 alone groups(2.30 ± 0.18, 2.18 ± 0.17, all P ＜ 0.05),the ROS levels of NaAsO2(40, 50 μmol/L) pretreated with 25 μmol/L tBHQ decreased than corresponding 5 μmol/L tBHQ pretreatment groups (all P ＜ 0.05). Conclusion tBHQ has a certain antagonism on arsenic induced cytotoxicity and oxidative injuries. \u0000 \u0000Key words: \u0000Arsenites;  Tert-butylhydroquinone;  Reactive oxygen species","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"34 1","pages":"489-492"},"PeriodicalIF":0.0,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82514852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomarkers of early vascular endothelial injury with low-arsenic exposure in drinking water","authors":"Chun-yan Ji, Chun Fu, Q. Xiang, Song Xu, Mingsheng Zhu, Jian Liu, Dapeng Wang, Jie Zhang, Yan An","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.05.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.05.006","url":null,"abstract":"Objective To observes the change of early effective biomarkers of endothelial injury with lowarsenic exposure in drinking water. Methods Ninety rurad residents, who had lived in Yanhe village, Xuyi county and Jiangsu province for at least 10 years, were recruited by simple random sampling in this study. The level of arsenic in their household shallow well were divided into three groups, which were ＜ 10 (32 people), 10 - 50(28 people) and ＞ 50 μg/L(30 people). Blood samples from individuals were collected. Malondialdehyde(MDA) in human plasma, which is considered as the most important marker for monitoring lipid peroxidation, was determined as conjugate with tetrabutylammonium hydrogen sulphate(TBA). The level of anti-superoxide anion radical(O-·2),C-reactive protein(CRP) and NO in human plasma was measured with colorimetry, turbidimetry and nitric acid reductase, respectively. The number of circulating endothelial progenitor cells(CEPCs) in peripheral blood was analyzed by CD133+/KDR+ antibodies and flow cytometry. Results Ninety cases underwent questionnaires. Between the groups, the difference of the levels of MDA (61.1, 65.5, 67.5 μmol/kg), O-·2 (4774.6, 5143.3, 4736.0 U/kg) ,CRP[(5.92 ± 2.44), (5.11 ± 2.40), (5.55 ± 2.96)mg/L], and NO[(659.8 ± 387.5), (667.4 ± 486.6), (762.1 ±763.2)μmol/kg], was not statistically significant (F =0.00, 0.46, 0.80, 0.47, all P ＞ 0.05). The difference of the number of CEPCs in different groups of arsenic in drinking water was statistically significant(0.96 x 10-5, 0.77 x 10-5,1.59 x 10-5, F=5.08, P＜ 0.05), where ＜ 10, 10 - 50 μg/L groups were significantly lower than ＞ 50 μg/L group (q =4.58, 6.65, all P ＜ 0.05). Conclusions The number of CEPCs in peripheral blood changes significantly with lower-arsenic exposure, whereas there are no obvious changes with the markers of oxidized damage and inflammation. This is the first human demonstration showing that lower-arsenic exposure may cause endothelial injury. \u0000 \u0000Key words: \u0000Arsenic ;  Endothelium, vascular;  Circulating endothelial progenitor cells;  Biomarkers","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"14 1","pages":"493-497"},"PeriodicalIF":0.0,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75269678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a method of improving the quality of sampling in review to determine the light areas of endemic fluorosis in quality control","authors":"Qiao Wang, Cheng-zhi Chen, He Yao, Haichao Zheng, Xue-jun Jiang, Kao-cong Tian","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.05.029","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.05.029","url":null,"abstract":"Objective To study an application of a method of improving the quality of sampling in review to determine the light areas of endemic fluorosis(referred to as endemic fluorosis) in quality control. Methods Of 15 light endemic fluorosis township(town), six were randomly sampled, and the prevalence of dental fluorosis in 22 village primary school children aged 8 to 12 were reviewed to determine the improved quality of sampling in Xuyong county Sichuan province. Results Six townships(towns) were selected by simple random sampling from 15 endemic fluorosis townships(towns) for review inspection in Xuyong country. A total of 22 villages were verified, accounting for 22.7％ of the total 97 villages verified. Of the 416 children for review inspection of dental fluorosis, 383 children were positive. The consistent rate of children' s dental fluorosis was 92.07％, and the verification to be slight villages was up to 21 endemic villages, accounting for 95.45％. Conclusions The application of a method of improving the quality of sampling can improve the efficiency of quality control, and improve the accuracy. It is a novel quality control method. \u0000 \u0000Key words: \u0000Sampling studies;  Fluoride poisoning;  Quality control","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"61 1","pages":"572-575"},"PeriodicalIF":0.0,"publicationDate":"2011-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81492363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and identification of recombinant vaccine Bifidobacterium bifidum pGEX-Sj14-3-3 of Schistosoma japonicum","authors":"Ning Zhang, Li Wengui","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.04.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.04.002","url":null,"abstract":"Objective To construct and identify recombinant vaccine Bifwlobacterium bifidum(Bb)pGEX-Sj14-3-3 of Schistosoma japonicum(Sj). Methods Total RNA was extracted from adult Sj, antigen encoding gene Sj14-3-3 was amplified by RT-PCR and cloned into Escherichia coli (E. coli)-Bb shuttle expression vector pGEX-1λT to construct recombinant plasmid pGEX-Sj14-3-3. The recombinant plasmid was transformed into E. coli BL21 (DE3).The plasmid was extracted and identified by using BamH I and EcoR I. Then pGEX-Sjl4-3-3 was electroporated into Bb to construct recombinant Bb (pGEX-Sj14-3-3) vaccine. The extracted plasmid of the recombinant Bb (pGEX-Sj14-3-3) vaccine was identified by PCR, and the size of the products was compared with Sj14-3-3 gene of adult worms.Results Sj14-3-3 of 399 bp in length was amplified by RT-PCR. The products were digested by BamH I and EcoR I , and the fragments length of plasmid pGEX-Sj14-3-3 vector was 4947 bp, and of Sj 14-3-3 gene was 399 bp.The product of 399 bp Sj14-3-3 gene was also amplified by PCR from template of the extracted plasmid of the recombinant Bb(pGEX-Sj14-3-3 ) vaccine. The size of the product obtained was just the same as expected.Conclusion The recombinant Bb(pGEX-Sj14-3-3) vaccine of Sj is successfully constructed. \u0000 \u0000Key words: \u0000Schistosoma japonicum; Bifidobacterium bifidum; Plasmids; Vaccines","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"3 1","pages":"357-360"},"PeriodicalIF":0.0,"publicationDate":"2011-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88301199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of apoptosis in hepatic injury of rat and patients with Clonorchis sinensis infection","authors":"Xiao-li Zhang, Yun-xia Dong, Su Han, Rong Da, Yihong Li, J. Shu, Fengmin Zhang","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.04.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.04.005","url":null,"abstract":"Objective To investigate the liver injury and pathological changes of rat and patients with Clonorchis sinensis(C, sinensis) infection, and to clarify the role of apoptosis in the injury induced by C. sinensis.Methods Wistar rats were divided into two group: 60 in infection group and 20 in control. The rats in infection group were infected with C. sinensis via oral feeding encysted cercaria;rats in control group were fed with normal saline. The rats were sacrificed 4, 6, 8 and 12 weeks after infection, respectively. Liver tissue specimens of the patients infected with C. sinensis were collected. The pathological changes of liver tissue were observed by light microscopy and the apoptofic rate of hepatocyte was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling(TUNEL) assay. Results Parasites and eggs could he seen around the bile duct, and the duct was associated with mucosa and adenoma papillary hyperplasia, wall thickening, inflammatory cell infiltration, a small amount of fibrous tissue hyperplasia, and periportal liver cells surrounded by a number of nuclear condensation, all these changes meant morphological characteristics of apoptosis. Apoptotic rates of liver cells in infection group 4, 6,8 and 12 weeks after infection were (7.15 ± 1.50)%,(11.61 ± 3.09)%,(13.21 ± 3.47)% and (11.26 ± 4.06)%,respectively, which was significantly higher than that in control group [(2.57 ± 0.72)%, (3.17 + 0.77)%, (3.67 ±0.96)% and (2.84 ± 0.87)%, t values were 4.45, 5.49, 5.95 and 4.74, respectively, all P < 0.01]. Conclusions These findings indicate that C, sinensis can stimulate both hepatoeytic apoptosis and degeneration which may he related to clinical manifestations and liver lesions in patients with clonorchiasis. \u0000 \u0000Key words: \u0000Clonorchis sinensis; Infection; Apoptosis; Hepatoeyte","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"93 1","pages":"368-370"},"PeriodicalIF":0.0,"publicationDate":"2011-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73739633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study of cartilage lesions and COLIXA 3 protein expression in rats cartilage with chronic fluorosis","authors":"L. Tang, Sheng-bin Bai, Ya-lou Zhang, Kai-tai Liu, Yue-Xin Zhang, J. Zhong","doi":"10.3760/CMA.J.ISSN.1000-4955.2011.04.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1000-4955.2011.04.010","url":null,"abstract":"Objective To explore whether different degrees of fluorosis influence the expression of cartilage COLIXA3 protein in fluorosis model rats. Methods Forty male Wistar rats 3 to 4 weeks old were randomly divided into 5 groups according to body mass, and these rats were fed with distilled water containing sodium fluoride(NaF) of 0(control), 25, 50, 100 and 150 mg/L for 6 months, respectively, in order to establish the animal model of drinking water type fluorosis. Pathomorphologieal changes of the osseous tissues of rats were analyzed under light microscope and transmission electron microscope, and the expression of COLIXA3 protein of femur metaphysis was examined by immunohistochemistry. Results HE staining showed different degrees of femoral metaphyseal ossification of cartilage in each experimental group, bone density increased, with sclerotic lesions of skeletal fluorosis. The control group showed no abnormal cartilage. Electron microscopy showed that the experimental groups with varying degrees of cartilage cell swelling, cell matrix fades, 50 mg/L group .showed hyperplasia, and 100,150 mg/L groups were observed with organelles decreased, part of the disintegration of the cartilage cell lacunae, lmmunohistochemical staining of rat chondrocytes COLIXA3 was positive, cytoplasm with brown granules, cartilage COLIXA3 protein expression(23.3 ± 4.5, 41.2 ± 5.6, 26.4 ~ 7.5) in the 25, 50 and 100 mg/L groups enhanced. Compared to the control group (6.1 ± 3.5), the expression of 50 and 100 mg/L groups was significantly increased, and the differences were statistically significant(all P 0.05). Conclusions There has pathological changes of sclerosing skeletal fluorosis in animal model. Low-dose fluoride promotes while high-dose inhibits cartilage cell proliferation. When fluorine concentration in external environment is too high and with extended exposure to fluoride, direct toxic effects of fluoride on cartilage cells is observed. Fluorine affects and promotes the expression of COLIXA3 protein in cartilage. Low-dose fluoride can promote COLIXA3 protein expression, as the dose increases (over 100 mg/L), the effect decreases. \u0000 \u0000Key words: \u0000Fluorosis, dental; Cartilage; COLIXA3 gene ; Rat","PeriodicalId":55880,"journal":{"name":"Chinese Journal of Endemiology","volume":"112 1","pages":"389-392"},"PeriodicalIF":0.0,"publicationDate":"2011-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87747343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}